Abstract:
:Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica napus L. AAPT1 gene (designated BnAAPT1) was identified from cDNA libraries of seedlings and developing seeds. Functional characterization was accomplished by heterologous expression of BnAAPT1 in a yeast strain deficient in AAPT activities. BnAAPT1 exhibited a greater preference for utilizing CDP-choline as a substrate with Vmax of 35 [14C]phosphatidylcholine nmol h(-1) mg(-1) protein and apparent Km of 32 microM while CDP-ethanolamine had a Vmax of 13 [14C]phosphatidylethanolamine nmol h(-1) mg(-1)protein and an apparent Km of 127 microM. The enzyme was activated by Mg2+, Mn2+ and phospholipid mixtures, and inhibited by Ca2+. A CDP-alcohol phosphotransferase motif, Asp99-Gly100-(X2)-Ala103-Arg104-(X8)-Gly113-(X3)-Asp117-(X3)-Asp121, was completely conserved in BnAAPT1 and its catalytic role was confirmed by scanning alanine mutagenesis. Over-expression of BnAAPT1 under the control of the double 35S promoter in transgenic Arabidopsis thaliana (L.) Heynh. plants led to elevated levels of the corresponding transcript and enzyme activity. In four of the high over-expression transgenic lines, phospholipid and fatty acid composition analyses revealed that chloroplastidic and extrachloroplastidic membranes isolated from transgenic leaves had about a 25% increase in phosphatidylcholine and in the proportions of polyunsaturated fatty acids [18:2+18:3], relative to the control. There were also consistent, but small differences observed in the proportions of 18:3 in transgenic green siliques and in 20:1 in mature transgenic seeds of these lines. Induction of Arabidopsis AAPT transcription in response to (+)-abscisic acid and low-temperature treatments, and the cold tolerance in BnAAPT1 transgenic seedlings implies that AAPT may play a role in resistance to damage at low growth temperatures.
journal_name
Plantajournal_title
Plantaauthors
Qi Q,Huang YF,Cutler AJ,Abrams SR,Taylor DCdoi
10.1007/s00425-003-1031-6subject
Has Abstractpub_date
2003-08-01 00:00:00pages
547-58issue
4eissn
0032-0935issn
1432-2048journal_volume
217pub_type
杂志文章相关文献
PLANTA文献大全abstract::Preharvest aflatoxin contamination has been identified by the peanut industry as a serious issue in food safety and human health because of the carcinogenic toxicity. Drought stress is the most important environmental factor exacerbating Aspergillus infection and aflatoxin contamination in peanut. The development of d...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0112-0
更新日期:2006-02-01 00:00:00
abstract:MAIN CONCLUSION:Loss of function mutation of rice OsACOS12 impairs lipid metabolism-mediated anther cuticle and pollen wall formation, and interferes with tapetum programmed cell death, leading to male sterility. Acyl-CoA Synthetase (ACOS) is one of the enzymes activating fatty acids for various metabolic functions in ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2691-y
更新日期:2017-07-01 00:00:00
abstract::Primary roots of maize (Zea mays L.) and pea (Pisum sativum L.) exhibit strong positive gravitropism. In both species, gravistimulation induces polar movement of calcium across the root tip from the upper side to the lower side. Roots of onion (Allium cepa L.) are not responsive to gravity and gravistimulation induc...
journal_title:Planta
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::From compartmental analysis of radioisotope elutin measurements, fluxes of Ca(2+) were estimated for cortical cells in root segments of onion, Allium cepa L., relative to complete nutrient solutions containing a range of calcium concentrations ([Ca0]) from 2 μeq l(-1) to 20 meq l(-1), increasing in 10-fold steps for C...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385353
更新日期:1981-08-01 00:00:00
abstract::Thermogenesis during the blooming of inflorescence is found in several but not all aroids. To understand what is critical for thermogenesis, we investigated the difference between thermogenic and non-thermogenic skunk cabbages (Symplocarpus renifolius and Lysichiton camtschatcensis), which are closely related in morph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-1034-z
更新日期:2009-12-01 00:00:00
abstract::The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic fo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385321
更新日期:1981-10-01 00:00:00
abstract::Two phosphorylases could be detected on gel-electropherograms of leaf-extracts of Spinacia oleracea and of immature cotyledons of Vicia faba. These two phosphorylases could be separated by means of ammonium sulfate fractionation. Both the slower migrating phosphorylases from spinach and from beans, but not the fast on...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00396028
更新日期:1968-09-01 00:00:00
abstract::The beta-ketoacyl-acyl carrier protein synthase III (KAS III; EC 2.3.1.180) is a condensing enzyme catalyzing the initial step of fatty acid biosynthesis using acetyl-CoA as primer. To determine the mechanisms involved in the biosynthesis of fatty acids in sunflower (Helianthus annuus L.) developing seeds, a cDNA codi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-010-1131-z
更新日期:2010-05-01 00:00:00
abstract::Seed plants with compound leaves constitute a polyphyletic group, but studies of diverse taxa show that genes of the class 1 KNOTTED-LIKE HOMEOBOX (KNOX1) family are often involved in compound leaf development. This suggests that knox1 genes have been recruited on multiple occasions during angiosperm evolution (Bharat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0288-y
更新日期:2006-10-01 00:00:00
abstract::Acid phosphatase has been localized by cytochemical techniques in aleurone layers of dry barley (Hordeum vulgare L.) grains, in imbibed half-grains and in isolated layers treated with and without gibberellic acid (GA3). A major fraction of the enzyme activity is located in the cell walls. During imbibition and incubat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388273
更新日期:1974-01-01 00:00:00
abstract::We used a heterologous system (transgenic Nicotiana tabacum L.) to investigate the processing, assembly and targeting of phytohemagglutinin (PHA), the lectin of the common bean, Phaseolus vulgaris L. In the bean, this glycoprotein accumulates in the protein bodies of the storage parenchyma cells in the cotyledons, and...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392425
更新日期:1988-08-01 00:00:00
abstract::Rapidly growing, green motile flagellates of Haematococcus pluvialis can transform into enlarged red resting cysts (aplanospores) under oxidative stress conditions. However, it is not known what initial molecular defense mechanisms occur in response to oxidative stress, and may ultimately lead to cellular transformati...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1323-5
更新日期:2004-11-01 00:00:00
abstract::Freshly isolated protoplasts or separated cells prepared from leaves of Nicotiana tabacum L. cvs. Xanthincor Turkish Samsun exhibit substantially greater incorporation of [(3)H] leucine and [(3)H] uridine into protoplast protein and RNA, respectively, when dilute rather than concentrated suspensions are incubated. Thi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387350
更新日期:1976-01-01 00:00:00
abstract::In a previous paper (HOCK, KüHNERT and MOHR, 1965) we have shown that fat degradation in the cotyledons of the mustard seedling (Sinapis alba L). is under the control of active phytochrome (=P730), at least to a certain extent. One could imagine that P730 might exert this control over fat consumption through isocitrit...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387481
更新日期:1966-06-01 00:00:00
abstract::Canavanine is an arginine analogue found in the seeds of many common legumes and is known to inhibit protein synthesis and growth in a number of organisms. Yet canavanine may comprise as much as 4% of the seed dry weight of the jackbean (Canavalia ensiformis).Canavanine is accumulated during earlier development in the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387456
更新日期:1967-06-01 00:00:00
abstract::Growth inhibitors were assayed from extracts of intact (attached) and of excised (cultured) root caps of Zea mays L., cv. Merit, the roots of which show a positive geotropic response only after exposure to light. If caps are intact at the time of illumination, at least two inhibitory substances are produced, an acid i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394989
更新日期:1981-12-01 00:00:00
abstract::Roots of marigold (Tagetes patula L.) accumulate thiophenes, heterocyclic sulfurous compounds with strong biocidal activity. In detached roots cultured in vitro, the thiophene content was 5 μmol·(g fresh weight)(-1) which is 25-times higher than in roots attached to the plant. In roots derived from tissues transformed...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395769
更新日期:1989-08-01 00:00:00
abstract::Leaf tissue of the panicoid grass Pennisetum purpureum (Schum) and of the chloridoid grass Muhlenbergia montana (Hitchcock) were fractionated to produce preparations enriched in the contents of mesophyll and bundle sheath cells. Sucrose phosphate synthetase and sucrose synthetase were found predominantly in the mesoph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385403
更新日期:1975-01-01 00:00:00
abstract::Indoleacetic acid buffered at pH 7.0 induces a high growth rate in Avena coleoptile segments after a latent period, the duration of which is dependent upon both IAA concentration and temperature. A minimum latent period of 7.3 min is observed at 25° C with 10(-3) M IAA in phosphate buffer at pH 7.0.In contrast, 5×10(-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387945
更新日期:1973-12-01 00:00:00
abstract::Colchicine has been demonstrated by ion-exchange and by gelfiltration assay to bind to a protein fraction derived from the higher plant Heracleum mantegazzianum. Colchicine-binding protein from a plant source was much more unstable than tubulin from animal preparations. The tissues of Heracleum vary in their content o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386122
更新日期:1973-06-01 00:00:00
abstract::O2-uptake and CO2-release by a chlorophyll-free, carotenoid-containing mutant of Chlorella vulgaris increase on addition of Na-glycolate by factors of 4-5 and 5-6, respectively (Fig. 1). In an enzyme preparation of that alga (sonification, centrifugation, precipitation with 0-30% (NH4)2SO4, dialysis) activity of glyco...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387441
更新日期:1971-09-01 00:00:00
abstract:MAIN CONCLUSION:UV - B radiation exposure for upto 3 h did not cause direct damage to physiology, but adjusted secondary metabolism and metabolites accumulation as an effective acclimation mechanism to mitigate the adverse effects of radiation. Artemisia annua L. plants were irradiated with UV-B radiation (280-315 nm; ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2114-2
更新日期:2014-09-01 00:00:00
abstract:MAIN CONCLUSION:Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family en...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2297-1
更新日期:2015-07-01 00:00:00
abstract::We have investigated events which take place in the developing barley (Hordeum vulgare L.) embryo during its acquisition of desiccation tolerance. Excised embryos are capable of precocious germination as early as 8 d after pollination (DAP). At this age, however, they are not capable of resisting a desiccation treatme...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393069
更新日期:1988-10-01 00:00:00
abstract::A method has been devised to ensure capture of large numbers of live paramecia within a short period of time under controlled conditions by the bladders of Utricularia gibba. The method permits a direct evaluation of the role of entrapped animals in the nutrition of this carnivorous plant. Paramecia captured by the bl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385021
更新日期:1968-06-01 00:00:00
abstract::1. The technique of streak photography was modified to use seven parallel cameras, each focused on an individual root in a guide holding flowing nutrient. Streak photographs representing displacement of points on the longitudinal axis of the root were projected on the table of an image plane digitizer. The displacemen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386959
更新日期:1969-03-01 00:00:00
abstract:MAIN CONCLUSION:Bambara groundnut has the potential to be used to contribute more the climate change ready agriculture. The requirement for nitrogen fixing, stress tolerant legumes is clear, particularly in low input agriculture. However, ensuring that existing negative traits are tackled and demand is stimulated throu...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-019-03191-6
更新日期:2019-09-01 00:00:00
abstract:MAIN CONCLUSION:Recent publications have increased our knowledge of how pectin composition and the degree of homogalacturonan methylesterification impact the biochemical and biomechanical properties of plant cell walls, plant development, and plants' interactions with their abiotic and biotic environments. Experimental...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-015-2358-5
更新日期:2015-10-01 00:00:00
abstract::The effects of the two botryticides, fludioxonil (fdx) and fenhexamid (fhd), were investigated on grapevine leaves (Vitis vinifera L. cv. Pinot noir) following photosynthesis and defense mechanisms. Treatments were carried out in vineyard at the end of flowering. Phytotoxicity of both fungicides was evaluated by measu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0849-3
更新日期:2009-02-01 00:00:00
abstract::This study tests the hypothesis that red-leaved gametophytes of the liverwort Jamesoniella colorata (Lehm.) Schiffn., which are found in relatively dry habitats, are more desiccation tolerant than their green counterparts, which are found in moister environments, through superior photoprotective systems. The potential...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0702-8
更新日期:2008-05-01 00:00:00