Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae).


:Rapidly growing, green motile flagellates of Haematococcus pluvialis can transform into enlarged red resting cysts (aplanospores) under oxidative stress conditions. However, it is not known what initial molecular defense mechanisms occur in response to oxidative stress, and may ultimately lead to cellular transformation. In this study, global-expression profiling of cellular proteins in response to stress was analyzed by two-dimensional gel electrophoresis, image analysis, and peptide mass fingerprinting. Oxidative stress was induced in cultures of green flagellates by addition of acetate and Fe2+, and exposure to excess light intensity. Overall, 70 proteins were identified with altered expression patterns following stress induction. Some key proteins involved in photosynthesis and nitrogen assimilation were down-regulated, whereas some mitochondrial respiratory proteins were transiently up-regulated after the onset of stress. Most of the identified proteins, particularly those from the families of superoxide dismutase, catalase, and peroxidase, were transiently up-regulated, but reverted to down-regulation during the 6 days of stress. On the other hand, cellular accumulation of the antioxidant astaxanthin occurred well after initiation of oxidative stress and reached its maximum cellular level after six or more days of stress. It appears that the early stress response involves multiple enzymatic defense processes that play a critical role upon onset of stress and also during the early transition of green vegetative cells to red cysts. As cyst development continues, the intensive, enzyme-mediated initial responses were largely replaced in mature red cysts by accumulation of the molecular antioxidant astaxanthin. This study provides the first direct evidence for a massive, and concerted up-regulation of multiple antioxidative defense mechanisms, both spatially and temporarily, to protect H. pluvialis cells against oxidative stress.






Wang SB,Chen F,Sommerfeld M,Hu Q




Has Abstract


2004-11-01 00:00:00














  • Phytochrome in etiolated annual rye : II. Distribution of photoreversible phytochrome in the coleoptile and primary leaf.

    abstract::Phytochrome per fresh weight in the apical 0.5 cm of the 1.5-cm coleoptile was twice that in the basal 0.5 cm; phytochrome per dry weight and phytochrome per DNA did not vary significantly from the base to the apex. Ratios of phytochrome to fresh or dry weight in the 6-cm coleoptile were nearly constant in the basal 5...


    pub_type: 杂志文章


    authors: Correll DL,Edwards JL,Medina VJ

    更新日期:1968-12-01 00:00:00

  • Synergistic interactions of CO2 enrichment and nitrogen deposition promote growth and ecophysiological advantages of invading Eupatorium adenophorum in Southwest China.

    abstract::Global environmental change and ongoing biological invasions are the two prominent ecological issues threatening biodiversity worldwide, and investigations of their interaction will aid to predict plant invasions and inform better management strategies in the future. In this study, invasive Eupatorium adenophorum and ...


    pub_type: 杂志文章


    authors: Lei YB,Wang WB,Feng YL,Zheng YL,Gong HD

    更新日期:2012-10-01 00:00:00

  • [Comparative studies on the lipid composition of etioplasts and chloroplasts from Pisum and of chloroplasts from an Aurea mutant of Nicotiana].

    abstract::Etioplasts and chloroplasts isolated from Pisum sativum differ in lipid composition. The relative amounts of galactolipids, especially of MGD, are higher in the chloroplast. In contrast, the sulfo- and the phospholipids are already present in the etioplasts in higher concentrations. During the formation of the thylako...


    pub_type: 杂志文章


    authors: Heise KP,Jacobi G

    更新日期:1973-06-01 00:00:00

  • A Moso bamboo gene VQ28 confers salt tolerance to transgenic Arabidopsis plants.

    abstract:MAIN CONCLUSION:Overexpression ofPeVQ28in Arabidopsis regulated the expression of salt/ABA-responsive genes and indicated thatPeVQ28may affect the ABA synthesis induced by stress in plants by regulating salt tolerance. Plant-specific VQ proteins, which contain a conserved short FxxhVQxhTG amino acid sequence motif, pla...


    pub_type: 杂志文章


    authors: Cheng X,Wang Y,Xiong R,Gao Y,Yan H,Xiang Y

    更新日期:2020-04-21 00:00:00

  • Isolation and expression analysis of partial sequences of heavy metal transporters from Brassica juncea by coupling high throughput cloning with a molecular fingerprinting technique.

    abstract::Heavy metal transporters play a key role in regulating metal accumulation and transport in plants. These are important candidate genes to study in metal tolerant and accumulator plants for their potential use in environmental clean up. We coupled a degenerate primer-based RT-PCR approach with a molecular fingerprintin...


    pub_type: 杂志文章


    authors: Das S,Sen M,Saha C,Chakraborty D,Das A,Banerjee M,Seal A

    更新日期:2011-07-01 00:00:00

  • Peptide growth factor phytosulfokine-alpha contributes to the pollen population effect.

    abstract::Density-dependent pollen germination and tube growth in vitro is a well-documented phenomenon, termed the pollen population effect, but far less is known about its molecular basis. We present evidence to support phytosulfokine-alpha [Y(SO3H)IY(SO3H)TQ; PSK-alpha] as a native bioactive factor contributing to this effec...


    pub_type: 杂志文章


    authors: Chen YF,Matsubayashi Y,Sakagami Y

    更新日期:2000-10-01 00:00:00

  • Formation of isopentenyl diphosphate via mevalonate does not occur within etioplasts and etiochloroplasts of mustard (Sinapis alba L.) seedlings.

    abstract::Seedlings from the white mustard, Sinapis alba, grown under continuous far-red light exhibit enhanced plastid enzyme activities when compared with dark-grown seedlings (for review, see Mohr 1981). These activities are even more pronounced upon illumination with white light during the etioplast/chloroplast transformati...


    pub_type: 杂志文章


    authors: Lütke-Brinkhaus F,Kleinig H

    更新日期:1987-07-01 00:00:00

  • Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion.

    abstract:MAIN CONCLUSION:A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion. Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most ...


    pub_type: 杂志文章


    authors: Pierce ET,Graham BP,Stiff MR,Osborne JA,Haigler CH

    更新日期:2019-05-01 00:00:00

  • Cellulose microfibril orientation and cell shaping in developing guard cells of Allium: The role of microtubules and ion accumulation.

    abstract::The role of microtubules and ions in cell shaping was investigated in differentiating guard cells of Allium using light and electron microscopy and cytochemistry. Microtubules appear soon after cytokinesis in a discrete zone close to the plasmalemma adjacent to the common wall between guard cells. The microtubules fan...


    pub_type: 杂志文章


    authors: Palevitz BA,Hepler PK

    更新日期:1976-01-01 00:00:00

  • Preferential polar pathways in the cuticle and their relationship to ectodesmata.

    abstract::Ectodesmata-like structures, referred to here as mercurous or mercury precipitates (MP) and considered to be identical to precipitates observed after treatment of leaf tissue with Gilson solution for demonstration of ectodesmata, were demonstrated with cuticle enzymatically isolated from Allium bulb scales and leaves ...


    pub_type: 杂志文章


    authors: Schönherr J,Bukovac MJ

    更新日期:1970-09-01 00:00:00

  • Biochemical analysis of elastic and rigid cuticles of Cirsium horridulum.

    abstract::The cuticle is a complex structure of soluble lipids, lipid polymers and polysaccharides. In addition to its functions to reduce water loss and provide a protective barrier, its mechanical properties may be significant to plant growth and development. We investigated the cuticle of Cirsium horridulum Michx. because of...


    pub_type: 杂志文章


    authors: Marga F,Pesacreta TC,Hasenstein KH

    更新日期:2001-10-01 00:00:00

  • Abscisic acid regulates pinoresinol-lariciresinol reductase gene expression and secoisolariciresinol accumulation in developing flax (Linum usitatissimum L.) seeds.

    abstract::Secoisolariciresinol diglucoside (SDG), the main phytoestrogenic lignan of Linum usitatissimum, is accumulated in the seed coat of flax during its development and pinoresinol-lariciresinol reductase (PLR) is a key enzyme in flax for its synthesis. The promoter of LuPLR1, a flax gene encoding a pinoresinol lariciresino...


    pub_type: 杂志文章


    authors: Renouard S,Corbin C,Lopez T,Montguillon J,Gutierrez L,Lamblin F,Lainé E,Hano C

    更新日期:2012-01-01 00:00:00

  • Influence of gravity and light on the developmental polarity of Ceratopteris richardii fern spores.

    abstract::The polarity of germinating single-celled spores of the fern Ceratopteris richardii Brogn. is influenced by gravity during a time period prior to the first cellular division designated a "polarity-determination window". After this window closes, control of polarity is seen in the downward (with respect to gravity) mig...


    pub_type: 杂志文章


    authors: Edwards ES,Roux SJ

    更新日期:1998-08-01 00:00:00

  • Actin-related defense mechanism to reject penetration attempt by a non-pathogen is maintained in tobacco BY-2 cells.

    abstract::The actin cytoskeleton is a key player in defense responses during early stages of infection by fungal pathogens. To investigate molecular mechanisms of actin-related defense responses, a cultured tobacco ( Nicotiana tabacum L.) BY-2 cell system was devised. When conidia were directly deposited on BY-2 cells, neither ...


    pub_type: 杂志文章


    authors: Kobayashi I,Hakuno H

    更新日期:2003-06-01 00:00:00

  • Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots.

    abstract::Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthet...


    pub_type: 杂志文章


    authors: Innocenti G,Pucciariello C,Le Gleuher M,Hopkins J,de Stefano M,Delledonne M,Puppo A,Baudouin E,Frendo P

    更新日期:2007-05-01 00:00:00

  • The plasma membrane of the Funaria caulonema tip cell: morphology and distribution of particle rosettes, and the kinetics of cellulose synthesis.

    abstract::Freeze-fracturing of Funaria hygrometrica caulonema cells leads to a cleavage within the plasma membrane. The extraplasmatic and the plasmatic fracture faces differ in their particle density. The plasmatic fracture face in caulonema tip cells or in tip cells of side branches, but never in other caulonema cells, is fur...


    pub_type: 杂志文章


    authors: Reiss HD,Schnepf E,Herth W

    更新日期:1984-04-01 00:00:00

  • Action potentials resulting from mechanical stimulation of pea epicotyls.

    abstract::Non-propagating, asynchronous action potentials may be detected with an extracellular electrode placed on the hook of the pea epicotyl following mechanical stimulation. These may well mediate the release of ethylene which has previously been shown to control the diameter of mechanically stressed shoots. ...


    pub_type: 杂志文章


    authors: Pickard BG

    更新日期:1971-06-01 00:00:00

  • Effect of four classes of herbicides on growth and acetolactate-synthase activity in several variants of Arabidopsis thaliana.

    abstract::We have isolated a triazolopyrimidine-resistant mutant csrl-2, of Arabidopsis thaliana (L.) Heynh. Here, we compare csrl-2 with the previously isolated mutants csrl and csr1-1, and with wild-type Arabidopsis for responses to members of four classes of herbicides, namely, sulfonylureas, triazolopyrimidines, imidazolino...


    pub_type: 杂志文章


    authors: Mourad G,King J

    更新日期:1992-11-01 00:00:00

  • Fatty-acid metabolism in senescing and regreening soybean cotyledons.

    abstract::Changes in fatty-acid metabolism were studied in soybean (Glycine max Merr.) cotyledons during senescence as well as in cotyledons which had been caused to regreen by removal of the epicotyl from the seedling. The activities of the enzymes acetyl-CoA synthetase (EC and fatty-acid synthetase in plastids isolat...


    pub_type: 杂志文章


    authors: Place MA,Morgan MS,Rutkoski A,Newman DW,Jaworski JG

    更新日期:1979-12-01 00:00:00

  • Increased root oxygen uptake in pea plants responding to non-self neighbors.

    abstract::Recent studies have demonstrated that plants alter root growth and decrease competition with roots of the same individual (self); however, the physiological traits accompanying this response are still widely unknown. In this study, we investigated the effect of root identity on gas exchange in the model species pea (P...


    pub_type: 杂志文章


    authors: Meier IC,Angert A,Falik O,Shelef O,Rachmilevitch S

    更新日期:2013-09-01 00:00:00

  • Iso-enzymes of acid ribonuclease in cotyledons of Pisum sativum L.

    abstract::Acid ribonuclease from cotyledons of Pisum sativum is very stable, with a temperature optimum of 65° C. It has a molecular weight of 17,500 and there is evidence that a fragment, with a molecular weight of 3,100, retains enzyme activity. Acid ribonuclease from the cotyledons of five-day old seedlings may be fractionat...


    pub_type: 杂志文章


    authors: Bryant JA,Greenway SC,West GA

    更新日期:1976-01-01 00:00:00

  • The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco expiants at a large range of concentrations.

    abstract::Short-term applications of very high concentrations of 1-naphthaleneacetic acid (NAA) to expiants from flower stalks of tobacco (Nicotiana tabacum L. cv. Samsun) induced flower-bud regeneration to the same extent as longer or continuous incubation on lower concentrations. The maximum number of flower buds per explant ...


    pub_type: 杂志文章


    authors: Smulders MJ,Visser EJ,Croes AF,Wullems GJ

    更新日期:1990-02-01 00:00:00

  • Flavonoid-related basic helix-loop-helix regulators, NtAn1a and NtAn1b, of tobacco have originated from two ancestors and are functionally active.

    abstract::The basic helix-loop-helix (bHLH) transcription factors (TFs) comprise one of the largest families of TFs involved in developmental and physiological processes in plants. Here, we describe the functional characterization of two bHLH TFs (NtAn1a and NtAn1b) isolated from tobacco (Nicotiana tabacum) flowers. NtAn1a and ...


    pub_type: 杂志文章


    authors: Bai Y,Pattanaik S,Patra B,Werkman JR,Xie CH,Yuan L

    更新日期:2011-08-01 00:00:00

  • The phenotype of the CRINKLY4 deletion mutant of Physcomitrella patens suggests a broad role in developmental regulation in early land plants.

    abstract:MAIN CONCLUSIONS:Deletion of the ancestral gene of the land plant multigene family of receptor like kinase CR4 in Physcomitrella patens demonstrates involvement in developmental control of gametophytic and sporophytic organs. The CRINKLY4 (CR4) family of receptor kinases in angiosperms consists of three clades, one inc...


    pub_type: 杂志文章


    authors: Demko V,Ako E,Perroud PF,Quatrano R,Olsen OA

    更新日期:2016-07-01 00:00:00

  • Expression patterns of cell wall-modifying enzymes during grape berry development.

    abstract::During ripening of grape (Vitis vinifera L.) berries, softening occurs concomitantly with the second growth phase of the fruit and involves significant changes in the properties of cell wall polysaccharides. Here, the activities of enzymes that might participate in cell wall modification have been monitored throughout...


    pub_type: 杂志文章


    authors: Nunan KJ,Davies C,Robinson SP,Fincher GB

    更新日期:2001-12-01 00:00:00

  • Sucrose transport in tonoplast vesicles of red beet roots is linked to ATP hydrolysis.

    abstract::Sucrose uptake into tonoplast vesicles, which were prepared from red beet (Beta vulgaris L.) vacuoles isolated by two different methods, was stimulated by MgATP. Using the same medium as for osmotic disruption of vacuoles, membrane vesicles were prepared from tissue homogenates of dormant red beet roots and separated ...


    pub_type: 杂志文章


    authors: Getz HP

    更新日期:1991-09-01 00:00:00

  • [Attraction of gibberellin by indoleacetic acid as a factor in apical dominance].

    abstract::Experiments with 20 cm long segments of etiolated pea epicotyls treated in the middle with 0,5% gibberellin paste (GA3) in the form of a ring and also with a 0,5% indoleacetic acid (IAA) paste either near the apical or near the basal pole showed that in all cases after 24 hours there was a stronger transport of GA3 to...


    pub_type: 杂志文章


    authors: Sebánek J,Hink J

    更新日期:1967-06-01 00:00:00

  • The role of amylomaltase in maltose metabolism in the cytosol of photosynthetic cells.

    abstract::Transitory starch is stored during the day inside chloroplasts and then broken down at night for export. Recent data indicate that maltose is the major form of carbon exported from the chloroplast at night but its fate in the cytosol is unknown. An amylomaltase gene ( malQ) cloned from Escherichia coli is necessary fo...


    pub_type: 杂志文章


    authors: Lu Y,Sharkey TD

    更新日期:2004-01-01 00:00:00

  • Changes in ultrastructure and transcription induced by elevated temperature in Zea mays embryonic root cells.

    abstract::A study has been made of the changes occurring in Zea mays kernels exposed to thermal shock at the 48th h of soaking at 16°C, i.e., just before the protrusion of the root. Heat shock of 5 h at 46°C temporarily inhibits the resumption of root growth and consequently retards the protrusion of the root. On the ultrastruc...


    pub_type: 杂志文章


    authors: Fransolet S,Deltour R,Bronchart R,Van de Walle C

    更新日期:1979-01-01 00:00:00

  • Isolation of novel types of Arabidopsis mutants with altered reactions to cadmium: cadmium-gradient agar plates are an effective screen for the heavy metal-related mutants.

    abstract::We are interested in elucidating the molecular mechanisms underlying plant reactions to the toxic heavy metal cadmium (Cd). To this end, we devised a new screening strategy using agar plates with a gradient of Cd concentrations, termed Cd-gradient agar plates (CGAPs), to isolate Arabidopsis mutants that displayed alte...


    pub_type: 杂志文章


    authors: Watanabe A,Ito H,Chiba M,Ito A,Shimizu H,Fuji S,Nakamura S,Hattori H,Chino M,Satoh-Nagasawa N,Takahashi H,Sakurai K,Akagi H

    更新日期:2010-09-01 00:00:00