Abstract:
:The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic for histidine, one clone for tryptophan and three clones for nicotinamide. Two clones that grow only in presence of a group of amino acids including glutamine and asparagine are also ClO 3 (-) resistant. Two further clones have as yet undefined amino-acid requirements. Two temperature-sensitive clones were found, one of which stops growing at the restrictive temperature of 32°C and the other undergoes chlorosis and accumulates an insoluble brown pigment. All clones expressed consistently the variant phenotypes in many retests and characterisation experiments over more than one year. Shoots have been regenerated from the nicotinamide- and histidine-requiring clones and from one temperaturesensitive clone. Two control (wild-type) morphogenic clones were used: one green and the other more variably pigmented and showing some growth stimulation in presence of medium supplements.
journal_name
Plantajournal_title
Plantaauthors
Gebhardt C,Schnebli V,King PJdoi
10.1007/BF00385321subject
Has Abstractpub_date
1981-10-01 00:00:00pages
81-9issue
1eissn
0032-0935issn
1432-2048journal_volume
153pub_type
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