Isolation of nuclei from rice tissue grown in suspension culture.

Abstract:

:A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.

journal_name

Planta

journal_title

Planta

authors

App A,Granados RR

doi

10.1007/BF00384931

subject

Has Abstract

pub_date

1974-01-01 00:00:00

pages

201-5

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

120

pub_type

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