Abstract:
:Cell-suspension cultures were initiated from embryogenic calli of various barley cultivars. Seven fast-growing suspension lines were obtained from four different cultivars (cvs. Dissa, Emir, Golden Promise and Igri). Two of these cell suspensions showed morphogenic capacity. From a cell suspension of cv. Dissa, albino plantlets were regenerated when aggregates were cultured on solid medium. Aggregates of cv. Igri usually stopped differentiation at the globular stage, but occasionally formed scutellum-like structures. Five suspension lines were used for protoplast isolation and culture. Dividing protoplasts were obtained from all lines, but with cv. Igri a few divisions only and no further development were observed. Protoplasts from the various lines differed in the time of first division (2-14 d), division frequency (0.09-70.9%) and efficiency of colony formation (0.09-7.3%). Protoplasts isolated from the morphogenic cell suspension of cv. Dissa developed compact calli which sporadically regenerated albino plantlets.
journal_name
Plantajournal_title
Plantaauthors
Lührs R,Lörz Hdoi
10.1007/BF00402883subject
Has Abstractpub_date
1988-07-01 00:00:00pages
71-81issue
1eissn
0032-0935issn
1432-2048journal_volume
175pub_type
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