Initiation of morphogenic cell-suspension and protoplast cultures of barley (Hordeum vulgare L.).

Abstract:

:Cell-suspension cultures were initiated from embryogenic calli of various barley cultivars. Seven fast-growing suspension lines were obtained from four different cultivars (cvs. Dissa, Emir, Golden Promise and Igri). Two of these cell suspensions showed morphogenic capacity. From a cell suspension of cv. Dissa, albino plantlets were regenerated when aggregates were cultured on solid medium. Aggregates of cv. Igri usually stopped differentiation at the globular stage, but occasionally formed scutellum-like structures. Five suspension lines were used for protoplast isolation and culture. Dividing protoplasts were obtained from all lines, but with cv. Igri a few divisions only and no further development were observed. Protoplasts from the various lines differed in the time of first division (2-14 d), division frequency (0.09-70.9%) and efficiency of colony formation (0.09-7.3%). Protoplasts isolated from the morphogenic cell suspension of cv. Dissa developed compact calli which sporadically regenerated albino plantlets.

journal_name

Planta

journal_title

Planta

authors

Lührs R,Lörz H

doi

10.1007/BF00402883

subject

Has Abstract

pub_date

1988-07-01 00:00:00

pages

71-81

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

175

pub_type

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