Formation of wheat protein bodies: Involvement of the Golgi apparatus in gliadin transport.

Abstract:

:Developing wheat (Triticum aestivum L.) endosperm was examined using ultrathin sections prepared from tissues harvested at 5, 9, 16 and 25 d after flowering. Protein bodies were evident by 9 d and displayed a variety of membranous structures and inclusions. The Golgi apparatus was a prominent organelle at all stages, and by 9 d was associated with small electron-dense inclusions. By immunocytochemical techniques, gliadin (wheat prolamine) was localized within these vesicles and in homogeneous regions of protein bodies, but not in the lumen of the rough endoplasmic reticulum. The protein bodies appear to enlarge by fusion of smaller protein bodies resulting in larger, irregular-shaped organelles. The affinity of the Golgi-derived vesicles for gliadin-specific probes during the period of maximal storage-protein synthesis and deposition indicates that this organelle includes the bulk, if not all, of the gliadin produced. The involvement of the Golgi apparatus in the packaging of gliadins into protein bodies indicates a pathway which differs from the mode of prolamine deposition in other cereals such as maize, rice and sorghum, and resembles the mechanism employed for the storage of rice glutelin and legume globulins.

journal_name

Planta

journal_title

Planta

authors

Kim WT,Franceschi VR,Krishnan HB,Okita TW

doi

10.1007/BF00392442

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

173-82

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

176

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Movement of Lucifer Yellow CH in potato tuber storage tissues: A comparison of symplastic and apoplastic transport.

    abstract::The fluorescent dye Lucifer Yellow CH (LYCH) was introduced directly into the symplast of potato (Solanum tuberosum L.) tuber storage parenchyma by microinjection and also into the apoplast through cuts made in the stolon cortex. Microinjected LYCH moved away rapidly from a single storage cell and spread radially via ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397661

    authors: Oparka KJ,Prior DA

    更新日期:1988-12-01 00:00:00

  • Emerson enhancement of carbon fixation but not of acetylene reduction (nitrogenase activity) in Anabaena cylindrica.

    abstract::Enhancement of carbon fixation was demonstrated in the bluegreen alga, Anabaena cylindrica, grown in either aerobic or microaerobic conditions. Under identical conditions no enhancement of acetylene reduction was observed. Light absorbed by photosystem I supported relatively more acetylene reduction than carbon fixati...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385450

    authors: Lyne RL,Stewart WD

    更新日期:1973-03-01 00:00:00

  • Composition, ultrastructure and function of the cutin- and suberin-containing layers in the leaf, fruit peel, juice-sac and inner seed coat of grapefruit (Citrus paradisi Macfed.).

    abstract::Cutin and suberin polymers from various anatomical regions of grapefruit were analyzed chemically and ultrastructurally. The leaf, fruit peel and juice-sac showed an amorphous cuticular layer. The cutin in the leaf was composed of 10,16-dihydroxy C16 acid and its positional isomers as the major monomers whereas 16-hyd...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385755

    authors: Espelie KE,Davis RW,Kolattukudy PE

    更新日期:1980-10-01 00:00:00

  • Molecular characterization of miRNA genes and their expression in Dimocarpus longan Lour.

    abstract:MAIN CONCLUSION:A genome-wide analysis of longan miRNA genes was conducted, and full-length pri-miRNA transcripts were cloned. Bioinformatics and expression analyses contributed to the functional characterization of longan miRNA genes. MicroRNAs are important for the post-transcriptional regulation of target genes. How...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-021-03564-w

    authors: Lin Y,Chen Y,Zeng Y,Zhang S,Zhang Z,Chen Y,Gong J,Lai Z

    更新日期:2021-01-21 00:00:00

  • Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions.

    abstract::Oilseed rape (Brassica napus L.) needs very high nitrogen fertilizer inputs. Significant amounts of this nitrogen are lost during early leaf shedding and are a source of environmental and economic concern. The objective of this study was to investigate whether the remobilization of leaf amino acids could be limiting f...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1446-8

    authors: Tilsner J,Kassner N,Struck C,Lohaus G

    更新日期:2005-06-01 00:00:00

  • Ribosomal RNA of Hyacinthus orientalis L. female gametophyte cells before and after fertilization.

    abstract::The nucleolar activity of Hyacinthus orientalis L. embryo sac cells was investigated. The distributions of nascent pre-rRNA (ITS1), 26S rRNA and of the 5S rRNA and U3 snoRNA were determined using fluorescence in situ hybridization (FISH). Our results indicated the different rRNA metabolism of the H. orientalis female ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1618-x

    authors: Niedojadło K,Pięciński S,Smoliński DJ,Bednarska-Kozakiewicz E

    更新日期:2012-07-01 00:00:00

  • Characterization of tomato endo-beta-1,4-glucanase Cel1 protein in fruit during ripening and after fungal infection.

    abstract::The tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase (EGase) Cel1 protein was characterized in fruit using specific antibodies. Two polypeptides ranging between 51 and 52 kDa were detected in the pericarp, and polypeptides ranging between 49 and 51 kDa were detected in locules. The polypeptides recognize...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1321-7

    authors: Real MD,Company P,García-Agustín P,Bennett AB,González-Bosch C

    更新日期:2004-11-01 00:00:00

  • Nitric oxide mediates humic acids-induced root development and plasma membrane H+-ATPase activation.

    abstract::It is widely reported that some humic substances behave as exogenous auxins influencing root growth by mechanisms that are not yet completely understood. This study explores the hypothesis that the humic acids' effects on root development involve a nitric oxide signaling. Maize seedlings were treated with HA 20 mg C L...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1106-0

    authors: Zandonadi DB,Santos MP,Dobbss LB,Olivares FL,Canellas LP,Binzel ML,Okorokova-Façanha AL,Façanha AR

    更新日期:2010-04-01 00:00:00

  • Nitrogenase activity, amino acid pool patterns and amination in blue-green algae.

    abstract::The free amino acid pools in the nitrogen-fixing blue-green algae Anabaena cylindrica, A. flos-aquae and Westiellopsis prolifica contain a variety of amino acids with aspartic acid, glutamic acid and the amide glutamine being present in much higher concentrations than the others. This pattern is characteristic of that...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386075

    authors: Dharmawardene MW,Stewart WD,Stanley SO

    更新日期:1972-06-01 00:00:00

  • Identification of genes differentially expressed in ectomycorrhizal roots during the Pinus pinaster-Laccaria bicolor interaction.

    abstract::Ectomycorrhizal associations are of major ecological importance in temperate and boreal forests. The development of a functional ectomycorrhiza requires many genetic and biochemical changes. In this study, suppressive subtraction hybridization was used to identify differentially expressed genes in the roots of maritim...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1874-4

    authors: Flores-Monterroso A,Canales J,de la Torre F,Ávila C,Cánovas FM

    更新日期:2013-06-01 00:00:00

  • Induction of transverse polarity by blue light: an all-or-none response.

    abstract::Phototropic stimulation induces a spatial memory. This was inferred from experiments with maize (Zea mays L.) coleoptiles involving opposing blue-light pulses, separated by variable time intervals, and rotation on a horizontal clinostat (Nick and Schafer, 1988b, Planta 175, 380-388). In those experiments, individual s...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201066

    authors: Nick P,Schafer E

    更新日期:1991-10-01 00:00:00

  • [Investigation on the regulation of the glycolate-2,6-dichlorophenolindophenol oxidoreductase activity during greening of a Chlorella mutant].

    abstract::We have examined the activity and regulation of the glycolate oxidizing enzyme in ten different greening stages of the Chlorella mutant Chlorella fusca C-1.1.10.10 The mutant has lost the ability to form chlorophyll in the dark. The dark grown cells exposed to light need after a short lag phase about 12 hs for normal ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388755

    authors: Wild A,Müller BV

    更新日期:1974-01-01 00:00:00

  • Replication and G2 checkpoints: their response to caffeine.

    abstract::Under long hydroxyurea treatments, evidence was obtained for the sequential activation of four checkpoints located between the onset of S phase and mitosis in Allium cepa L. root meristems. Biparametric flow cytometry (Br-DNA/total DNA) showed that cells initially accumulated at early S phase but, after a delay, they ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000415

    authors: Pelayo HR,Lastres P,De la Torre C

    更新日期:2001-02-01 00:00:00

  • Dynamics of plasmodesmal connectivity in successive interfaces of the cambial zone.

    abstract::Frequency, density and branching of plasmodesmata were counted in successive tangential and transverse walls in the cambial zone of tomato stems in order to examine development of the plasmodesmal network in a chronological order. Coincident with progress of cell development, plasmodesmal connectivity increased, both ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-1046-8

    authors: Ehlers K,van Bel AJ

    更新日期:2010-01-01 00:00:00

  • Cytophotometric investigation of DNA and RNA content in nuclei of active Strasburger cells in Pinus nigra var. austriaca (Hoess) Badoux.

    abstract::The nuclei of active, sieve cell-associated Strasburger cells in the secondary phloem of Pinus nigra var. austriaca (Hoess) Badoux have been studied for their structure and DNA and RNA content. No difference in size compared to those of ordinary ray cells was found. The nuclear surface is often increased by an ameboid...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394427

    authors: Sauter JJ,Ulrich H

    更新日期:1977-01-01 00:00:00

  • Characteristic symptoms of photosynthesis inhibition by herbicides are expressed in photomixotrophic tissue cultures of Nicotiana.

    abstract::A photomixotrophic tissue culture system for Nicotiana plumbaginifolia and N. tabacum has been developed in which a primary symptom (bleching) of the inhibition of photosynthetic electron transport by herbicides can be observed. Photomixotrophic cultures were initiated and maintained in the light on medium containing ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00407004

    authors: Cséplő A,Medgyesy P

    更新日期:1986-05-01 00:00:00

  • Evidence for an intra- and extraplastidic pre-chorismate pathway.

    abstract::Pea plants grown under different conditions of cultivation, and eight different plant species with variegated leaves were used to study the intracellular localization of shikimate oxidoreductase (EC 1.1.1.25), the marker enzyme of the pre-chorismate pathway. The two series of experiments indicated an intra-and an extr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397408

    authors: Rothe GM,Hengst G,Mildenberger I,Scharer H,Utesch D

    更新日期:1983-07-01 00:00:00

  • Occurence of nucleoside polyphosphates in rapidly labelled RNA preparations from radish seedlings (Raphanus sativus.).

    abstract::Preparations of rapidly labelled RNA are usually heavily contaminated by rapidly labelled low molecular weight components. This contaminating fraction mainly contains nucleoside triphosphates, as previously reported, but also nucleoside tetra, penta and hexaphosphates. These highly phosphorylated nucleosides are, acco...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385426

    authors: Aspart-Pascot L,Delseny M,Guitton Y

    更新日期:1976-01-01 00:00:00

  • [Fine structure of sieve-tube plastids of Aristolochia and Asarum (Aristolochiaceae)].

    abstract::Sieve-tube plastids of Aristolochia (5 species investigated) contain several starch grains and always one large crystalloid. In Asarum (3 species investigated) starch has not been found in the sieve-tubes. Their plastids contain several cuneate crystalloids that are sometimes arranged around an invisible centre. Asaru...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388406

    authors: Behnke HD

    更新日期:1971-03-01 00:00:00

  • [Ferredoxin from Bumilleriopsis filiformis Vischer].

    abstract::Ferredoxin from the alga Bumilleriopsis filiformis Vischer (Xanthophyceae) is characterized by comparing some of its properties with spinach ferredoxin.It is similar to the known plant ferredoxins in the following points: a) Absorption spectrum. b) Molecular weight. c) Amino acid composition (approx. 100 amino acid re...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385204

    authors: Böger P

    更新日期:1970-06-01 00:00:00

  • Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.).

    abstract::Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growt...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00982919

    authors: Ernst D,Oesterhelt D,Schäfer W

    更新日期:1984-05-01 00:00:00

  • Composition and properties of the sexual agglutinins of the flagellated green alga Chlamydomonas eugametos.

    abstract::Sexual interaction between gametes of opposite mating type (mt) of the unicellular green alga Chlamydomonas eugametos starts with agglutination of the cells via particular glycoproteins on the flagellar surface. Purification of these socalled agglutinins was achieved by a three-step procedure consisting of, successive...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00395022

    authors: Samson MR,Klis FM,Homan WL,van Egmond P,Musgrave A,van den Ende H

    更新日期:1987-03-01 00:00:00

  • Effect of 2,4-dichlorophenoxyacetic acid on the structure and function of developing chloroplasts.

    abstract::Dark-grown radish seedlings (Raphanus sativus L.) were sprayed with 10(-3) mol·l(-1) 2,4-dichlorophenoxyacetic acid and then were exposed to a 14:10 light: dark cycle. Cotyledon samples from these seedlings and unsprayed controls were taken for electron microscopy, chlorophyll determinations, and photosynthetic rate m...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394437

    authors: Nadakavukaren MJ,McCracken DA

    更新日期:1977-01-01 00:00:00

  • The light-induced reduction of the gravitropic growth-orientation of seedlings of Arabidopsis thaliana (L.) Heynh. is a photomorphogenic response mediated synergistically by the far-red-absorbing forms of phytochromes A and B.

    abstract::Hypocotyls of dark-grown seedlings of Arabidosis thaliana exhibit a strong negative gravitropism, which is reduced by red and also by long-wavelength, far-red light treatments. Light treatments using phytochrome A (phyA)- and phytochrome B (phyB)-deficient mutants showed that this response is controlled by phyB in a r...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00195180

    authors: Poppe C,Hangarter RP,Sharrock RA,Nagy F,Schäfer E

    更新日期:1996-01-01 00:00:00

  • The effect of light on chlorophyll loss in senescing leaves of sycamore (Acer pseudoplatanus L.).

    abstract::Breakdown of chlorophylls in attached senescing sycamore leaves held in darkness was significantly less over a 14-d period than that occurring in leaves exposed to natural light. Chlorophyll a declined more rapidly than chlorophyll b in both situations, the stability of the latter being particularly increased in darkn...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397332

    authors: Maunders MJ,Brown SB

    更新日期:1983-08-01 00:00:00

  • Day-night changes in leaf water relations associated with the rhythm of crassulacean acid metabolism in Kalanchoë daigremontiana.

    abstract::A study was made of the day-night changes under controlled environmental conditions in the bulk-leaf water relations of Kalanchoë daigremontiana, a plant showing Crassulacean acid metabolism. In addition to nocturnal stomatal opening and net CO2 uptake, the leaves of well-watered plants showed high rates of gas exchan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00393518

    authors: Smith JA,Lüttge U

    更新日期:1985-02-01 00:00:00

  • Inhibition of plant cell membrane transport phenomena induced by zearalenone (F-2).

    abstract::Zearalenone (F-2), an estrogenic factor produced by a number of Fusarium spp., stimulates a leakage of electrolytes, β-cyanin and aminoacids from three plant tissues. F-2 inhibits rubidium uptake in roots of Zea mays L. and Beta vulgaris L. var. rubra. However the effect in the latter tissue is evident after long-term...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00389051

    authors: Vianello A,Macri F

    更新日期:1978-01-01 00:00:00

  • Peptide growth factor phytosulfokine-alpha contributes to the pollen population effect.

    abstract::Density-dependent pollen germination and tube growth in vitro is a well-documented phenomenon, termed the pollen population effect, but far less is known about its molecular basis. We present evidence to support phytosulfokine-alpha [Y(SO3H)IY(SO3H)TQ; PSK-alpha] as a native bioactive factor contributing to this effec...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000370

    authors: Chen YF,Matsubayashi Y,Sakagami Y

    更新日期:2000-10-01 00:00:00

  • Photoregulation of transfer and 5S ribosomal RNA synthesis in Chlorella.

    abstract::The effect of blue and red light on the synthesis of transfer and 5S ribosomal RNA in autotrophic cultures of Chlorella pyrenoidosa was studied by pulse labeling experiments with tritiated guanosine. Compared with darkness or red light (679 nm), blue light (457 nm) of low intensities (quantum flux: 0.5-5×10(-10) mol p...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387550

    authors: Steup M,Ssymank V,Winkler U,Glock H

    更新日期:1977-01-01 00:00:00

  • The tonoplast functioning as the master switch for circadian regulation of crassulacean acid metabolism.

    abstract::From the initial discovery of free-running endogenous circadian oscillations of Crassulacean acid metabolism (CAM) under constant conditions in the light and in air, it has been disputed whether the underlying oscillator is enzymic or biophysical. The hypothesis of a biophysical hysteresis switch or beat oscillator st...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s004250000408

    authors: Lüttge U

    更新日期:2000-11-01 00:00:00