Replication and G2 checkpoints: their response to caffeine.

Abstract:

:Under long hydroxyurea treatments, evidence was obtained for the sequential activation of four checkpoints located between the onset of S phase and mitosis in Allium cepa L. root meristems. Biparametric flow cytometry (Br-DNA/total DNA) showed that cells initially accumulated at early S phase but, after a delay, they resumed replication and paused again at mid S phase. Cells not only overrode this second replication block but also any G2 checkpoint they encountered. Thus, a late mitotic wave was produced in the presence of hydroxyurea. The wave was formed by cells that had apparently completed their replication (normal mitoses), while others displayed anaphases/telophases with less than the expected DNA content and with chromosomal breaks (aberrant mitoses). The presence of aberrant mitoses is direct evidence for the undue override of the two G2 checkpoints responsible for surveillance of completion of DNA synthesis and repair, respectively. Caffeine selectively abrogated the G2 block produced by the checkpoint that controls post-replication DNA repair, as it advanced the entry of cells into an aberrant mitosis. However, caffeine proved not to be the universal checkpoint-evading agent as postulated. Caffeine did not modify the spontaneous override of the replication checkpoints. Moreover, it seems to enforce the checkpoint that controls the completion of DNA synthesis, as the appearance of the late wave of normal mitoses produced in the presence of hydroxyurea was prevented by the use of caffeine.

journal_name

Planta

journal_title

Planta

authors

Pelayo HR,Lastres P,De la Torre C

doi

10.1007/s004250000415

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

444-53

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

212

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Differential accumulation of the 10-, 16- and 23-kDa peripheral components of the water-splitting complex of photosystem II in mesophyll and bundle-sheath chloroplasts of the dicotyledonous C4 plant Flaveria trinervia (Spreng.) C. Mohr.

    abstract::The differential expression of PSII genes was investigated in mesophyll and bundle-sheath cells of Flaveria trinervia, a dicotyledonous C4 plant of the NADP-malic enzyme type. A comprehensive immunoblot analysis showed that three extrinsic proteins of the water-splitting complex (10, 16 and 23 kDa) are selectively dep...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00196260

    authors: Höfer MU,Santore UJ,Westhoff P

    更新日期:1992-01-01 00:00:00

  • Phloem unloading in aerial roots of Monstera deliciosa.

    abstract::Plants of Monstera deliciosa Liebm. pruned to exemplars with one leaf and one aerial root were labeled with 7.4 MBq (14)CO2 over the leaf blade. Microautoradiographs of soluble and insoluble radioactivity were prepared from three different regions of the aerial root. In addition, histochemical localization of ATPase w...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00396886

    authors: Eschrich W

    更新日期:1983-05-01 00:00:00

  • Xyloglucan O-acetyltransferases from Arabidopsis thaliana and Populus trichocarpa catalyze acetylation of fucosylated galactose residues on xyloglucan side chains.

    abstract:MAIN CONCLUSION:AXY4/XGOAT1, AXY4L/XGOAT2 and PtrXGOATs are O-acetyltransferases acetylating fucosylated galactose residues on xyloglucan and AXY9 does not directly catalyze O-acetylation of xyloglucan but exhibits weak acetylesterase activity. Xyloglucan is a major hemicellulose that cross-links cellulose in the prima...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2972-0

    authors: Zhong R,Cui D,Ye ZH

    更新日期:2018-11-01 00:00:00

  • Methylboronic acid fertilization alleviates boron deficiency symptoms in Arabidopsis thaliana.

    abstract:MAIN CONCLUSION:Our results showed that methylboronic acid is capable of alleviating boron deficiency, enhancing plant growth, and is less toxic than boric acid at higher concentrations. Boron is an essential plant micronutrient and its deficiency occurs in several regions globally, resulting in impaired plant growth. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2903-0

    authors: Duran C,Arce-Johnson P,Aquea F

    更新日期:2018-07-01 00:00:00

  • Scanning electron-microscopy of abscission-zone surfaces of Valencia-orange fruit.

    abstract::The scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of Valencia orange. Separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. The most noticeable differences are the lessened amount of tearing in pr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390215

    authors: Rogers BJ

    更新日期:1971-12-01 00:00:00

  • Sucrose storage in cell suspension cultures of Saccharum sp. (sugarcane) is regulated by a cycle of synthesis and degradation.

    abstract::We have investigated the regulation of sucrose storage in cell-suspension cultures of sugarcane. When grown in batch culture, sucrose accumulation commences after about 5 d, when the nitrogen supply is exhausted. Sucrose storage is also induced by decreasing the nitrogen supply to cells growing in a chemostat. The mea...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197564

    authors: Wendler R,Veith R,Dancer J,Stitt M,Komor E

    更新日期:1991-12-01 00:00:00

  • Cytosolic cycles regulate the turnover of sucrose in heterotrophic cell-suspension cultures of Chenopodium rubrum L.

    abstract::We have investigated whether sucrose accumulation in heterotrophic cell-suspension cultures of Chenopodium rubrum L. is regulated by a cycle in which sucrose is simultaneously synthesised and degraded. Net sucrose accumulation was measured by monitoring the sucrose content, unidirectional synthesis was monitored by su...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197115

    authors: Dancer J,Hatzfeld WD,Stitt M

    更新日期:1990-09-01 00:00:00

  • Cortical cell fluxes and transport to the stele in excised root segments of Allium cepa L. : IV. Calcium as affected by its external concentration.

    abstract::From compartmental analysis of radioisotope elutin measurements, fluxes of Ca(2+) were estimated for cortical cells in root segments of onion, Allium cepa L., relative to complete nutrient solutions containing a range of calcium concentrations ([Ca0]) from 2 μeq l(-1) to 20 meq l(-1), increasing in 10-fold steps for C...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385353

    authors: Macklon AE,Sim A

    更新日期:1981-08-01 00:00:00

  • C4-Pathway photosynthesis in Portulaca oleracea and the significance of alanine labelling.

    abstract::Portulaca oleracea L. has some features common to C4 species but it has been suggested from other studies that a substantial proportion of assimilated carbon dioxide is incorporated into pyruvate and alanine by routes not involving C4 acids or 3-phosphoglycerate. The present paper reports that enzyme activities in P. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385603

    authors: Hatch MD

    更新日期:1975-01-01 00:00:00

  • Photosynthetic activity of isolated chloroplasts from Euglena gracilis.

    abstract::Functional chloroplasts from photoheterotrophic Euglena gracilis can be isolated in isoosmotic gradients of 10-80% Percoll. The chloroplasts display rates of CO2 dependent O2 evolution and CO2 fixation of 30-50 μmol mg(-1) chlorophyll h(-1) or 25-35% of the net O2 evolution by the whole cells and appear to be striking...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385499

    authors: Schürmann P,Ortiz W

    更新日期:1982-03-01 00:00:00

  • Different properties of two types of auxin-binding sites in membranes from maize coleoptiles.

    abstract::Two types of auxin-binding sites (sites I and II) in membranes from maize (Zea mays L.) coleoptiles were characterized. Site I was a protein with a relative molecular mass of 21 000, and the distribution of site I protein on sucrose density gradient fractionation coincided with that of NADH-cytochrome-c reductase (EC ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00393079

    authors: Shimomura S,Inohara N,Fukui T,Futai M

    更新日期:1988-10-01 00:00:00

  • Is a decreased water potential after withholding oxygen to roots the cause of the decline of leaf-elongation rates in Zea mays L. and Phaseolus vulgaris L.?

    abstract::Leaf-elongation rates of Zea mays L. and Phaseolus vulgaris L. were measured in plants grown for 4 d in nutrient solution bubbled with N2 and in soil-grown waterlogged Phaseolus plants. Leaf water potential in both species was lower 3-4h after replacing aeration by N2-bubbling. In Zea, the water potential after 24 h o...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392806

    authors: Schildwacht PM

    更新日期:1989-02-01 00:00:00

  • Calmodulin and wound healing in the coenocytic green alga Ernodesmis verticillata (Kützing) Børgesen: Ultrastructure of the cortical cytoskeleton and immunogold labeling.

    abstract::Ultrastructural changes in the cortical cytoskeleton during wound-induced cytoplasmic contraction were examined in the coenocytic green alga Ernodesmis verticillata. Both calmodulin (CaM) and actin were localized in intact and contracting cells by immunogold labeling. Within 5 min after wounding, compact microfilament...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201493

    authors: Goddard RH,La Claire JW 2nd

    更新日期:1991-12-01 00:00:00

  • Chlorophyllase activity in developing leaves of Phaseolus vulgaris L.

    abstract::In crude extracts of primary leaves from dark grown seedlings of Phaseolus vulgaris L., relatively high hydrolytic activity of chlorophyllase (chlorophyll-chlorophyllido-hydrolase, EC 3.1.1.14) was observed. When plants were exposed to continuous illumination, the enzyme activity in the extracts was doubled within 3 d...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390814

    authors: Moll WA,de Wit B,Lutter R

    更新日期:1978-01-01 00:00:00

  • Topochemical and morphological characterization of wood cell wall treated with the ionic liquid, 1-ethylpyridinium bromide.

    abstract::MAIN CONCLUSION : [EtPy][Br] is more reactive toward lignin than toward the PSs in wood cell walls, and [EtPy][Br] treatment results in inhomogenous changes to the cell wall's ultrastructural and chemical components. The effects of the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]), which prefers to react with li...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2235-7

    authors: Kanbayashi T,Miyafuji H

    更新日期:2015-09-01 00:00:00

  • Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll.

    abstract::Vacuoles were isolated by osmotic rupture of mesophyll protoplasts from the primary leaves of 4-d- and 7-d-old plants of rye (Secale cereale L.). Their content of two flavones, luteolin 7-O-[β-D-glucuronosyl-(1→2)β-D-glucuronide] (R2) and luteolin 7-O-[β-D-glucuronosy 1 (1→2) β-D-glucuronide]-4'-O-β-D-glucuronide (R1)...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201627

    authors: Anhalt S,Weissenböck G

    更新日期:1992-04-01 00:00:00

  • cDNA cloning and expression of isoflavonoid-specific glucosyltransferase from Glycyrrhiza echinata cell-suspension cultures.

    abstract::A cDNA encoding UDP-glucose: formononetin 7- O-glucosyltransferase, designated UGT73F1, was cloned from yeast extract-treated Glycyrrhiza echinata L. cell-suspension cultures using probes from Scutellaria baicalensis UDP-glucose: flavonoid 7- O-glucosyltransferase. The open reading frame of the UGT73F1 cDNA encodes a ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1118-0

    authors: Nagashima S,Inagaki R,Kubo A,Hirotani M,Yoshikawa T

    更新日期:2004-01-01 00:00:00

  • Characterization of germination-specific lipid transfer proteins from Euphorbia lagascae.

    abstract::The endosperm of Euphorbia lagascae Spreng. seeds contains high levels of the epoxidated fatty acid vernolic acid ( cis-12-epoxyoctadeca-cis-9-enoic acid). To obtain transgenic oilcrops producing high levels of vernolic acid, better knowledge of its endogenous metabolism is needed. In this paper we study the gene acti...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-001-0717-x

    authors: Edqvist J,Farbos I

    更新日期:2002-05-01 00:00:00

  • Organization of cortical microtubules and microfibril deposition in response to blue-light-induced apical swelling in a tip-growing Adiantum protonema cell.

    abstract::The arrangements of cortical microtubules (MTs) in a tip-growing protonemal cell of Adiantum capillus-veneris L. and of cellulose microfibrils (MFs) in its wall were examined during blue-light (BL)-induced apical swelling. In most protonemal cells which had been growing in the longitudinal direction under red light, a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391861

    authors: Murata T,Wada M

    更新日期:1989-06-01 00:00:00

  • Transcriptome analysis of Panax zingiberensis identifies genes encoding oleanolic acid glucuronosyltransferase involved in the biosynthesis of oleanane-type ginsenosides.

    abstract:MAIN CONCLUSION:Oleanolic acid glucuronosyltransferase (OAGT) genes synthesizing the direct precursor of oleanane-type ginsenosides were discovered. The four recombinant proteins of OAGT were able to transfer glucuronic acid at C-3 of oleanolic acid that yields oleanolic acid 3-O-β-glucuronide. Ginsenosides are the pri...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2995-6

    authors: Tang QY,Chen G,Song WL,Fan W,Wei KH,He SM,Zhang GH,Tang JR,Li Y,Lin Y,Yang SC

    更新日期:2019-02-01 00:00:00

  • Identification and deletion analysis of the promoter of the pepper SAR8.2 gene activated by bacterial infection and abiotic stresses.

    abstract::The pepper SAR8.2 gene, CASAR82A, was locally and systemically induced in pepper plants which had been infected by Xanthomonas campestris pv. vesicatoria or by Pseudomonas fluorescens. The DNA 1,283 bp sequence upstream of the CASAR82A gene was assessed with regard to the activity of the CASAR82A promoter fused to the...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0210-z

    authors: Lee SC,Hwang BK

    更新日期:2006-07-01 00:00:00

  • Identification and localization of transformed cells in agrobacterium tumefaciens-induced plant tumors

    abstract::Agrobacterium tumefaciens-induced tumors of dicotyledonous plants consist of well-defined vascular bundle-like structures originating from transformed cells. The current view that 25% of the tumor cells are transformed has been re-investigated by using beta-glucuronidase (gus)-gene-containing wild-type bacteria (A281 ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050742

    authors: Rezmer C,Schlichting R,Wachter R,Ullrich CI

    更新日期:1999-10-01 00:00:00

  • PAP genes are tissue- and cell-specific markers of chloroplast development.

    abstract:MAIN CONCLUSION:Expression of PAP genes is strongly coordinated and represents a highly selective cell-specific marker associated with the development of chloroplasts in photosynthetically active organs of Arabidopsis seedlings and adult plants. Transcription in plastids of plants depends on the activity of phage-type ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2924-8

    authors: Liebers M,Chevalier F,Blanvillain R,Pfannschmidt T

    更新日期:2018-09-01 00:00:00

  • The influence of microbial contamination of fresh and washed beetroot disks on their capacity to absorb phosphate.

    abstract::When plant storage tissue is cut into thin disks it rapidly develops a high capacity to absorb phosphate from dilute solutions. The recent trend has been to attribute the development of this ability to the increased metabolic activity of bacteria known to be containing the tissue. Data presented in this paper shows th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387651

    authors: Palmer JM

    更新日期:1970-03-01 00:00:00

  • Model systems for the immunolocalisation of cis, trans abscisic acid in plant tissues.

    abstract::To explore the feasibility of immunolocalisation of endogenous abscisic acid (ABA), model systems were developed for testing quantitatively the sensitivity of the second antibody peroxidase/antiperoxidse (PAP) method for immunolocalisation of ABA on plant tissues. Exogenous (±)ABA was fixed to carrot sections on glass...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394587

    authors: Skene DS,Browning G,Jones HG

    更新日期:1987-10-01 00:00:00

  • Genome-wide analysis of pentatricopeptide-repeat proteins of an aquatic plant.

    abstract:MAIN CONCLUSION:A large proportion of genes in plant genomes are organized as gene families. Whereas most gene families in the aquative plant Spirodela are reduced in their copy number, the PPR gene family is expanded, which match the RNA editing sites in organelles, providing us with new insights in the evolution of f...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-016-2555-x

    authors: Wang W,Wu Y,Messing J

    更新日期:2016-10-01 00:00:00

  • The role of peroxidase isoenzyme groups of Nicotiana tabacum in hydrogen peroxide formation.

    abstract::Three peroxidase isoenzyme-groups found in cell walls of tobacco were tested for their capacity to form H2O2. Isoenzyme-group GI, located only in cell walls (GII and GIII are also found in protoplasts) showed the highest Kapp-value for H2O2-formation. The lowest Kapp-value, i.e., maximal H2O2-formation was received fo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00380189

    authors: Mäder M,Ungemach J,Schloß P

    更新日期:1980-02-01 00:00:00

  • A model of carbon dioxide assimilation in Chlamydomonas reinhardii.

    abstract::A simple model of photosynthetic CO2 assimilation in Chlamydomonas has been developed in order to evaluate whether a CO2-concentrating system could explain the photosynthetic characteristics of this alga (high apparent affinity for CO2, low photorespiration, little O2 inhibition of photosynthesis, and low CO2 compensa...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00402942

    authors: Spalding MH,Portis AR Jr

    更新日期:1985-06-01 00:00:00

  • Heat modification of ribulose-1,5-bisphosphate carboxylase/oxygenase by temperature pretreatment of wheat (Triticum aestivum L.) seedlings.

    abstract::The effect of low-, ambient- and high-temperature pretreatments (48 h at 4° C, 20° C or 36° C) of wheat seedlings (spring wheat Triticum aestivum L., cv. Kolibri) on the solubility properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase; EC 4.1.1.39) was studied. The extractable protein moiety of heat-...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397394

    authors: Weidner M,Fehling E

    更新日期:1985-09-01 00:00:00

  • Xylan synthetase activity in differentiated xylem cells of sycamore trees (Acer pseudoplatanus).

    abstract::Particulate enzymic preparations obtained from homogenates of differentiated xylem cells isolated from sycamore trees, catalyzed the formation of a radioactive xylan in the presence of UDP-D-[U-(14)C]xylose as substrate. The synthesized xylan was not dialyzable through Visking cellophane tubing. Successive extraction ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384237

    authors: Dalessandro G,Northcote DH

    更新日期:1981-01-01 00:00:00