Abstract:
:A cDNA encoding UDP-glucose: formononetin 7- O-glucosyltransferase, designated UGT73F1, was cloned from yeast extract-treated Glycyrrhiza echinata L. cell-suspension cultures using probes from Scutellaria baicalensis UDP-glucose: flavonoid 7- O-glucosyltransferase. The open reading frame of the UGT73F1 cDNA encodes a 441-amino-acid protein with a predicted molecular mass of 48.7 kDa. The deduced amino acid sequence showed that the protein is related to the stress-inducible glucosyltransferases. UGT73F1 mRNA was not detected in untreated G. echinata cultures but was transiently induced by treatment with yeast extract. Recombinant UGT73F1 was expressed as a histidine-tag fusion protein in Escherichia coli and purified to near homogeneity by nickel chelate chromatography. The purified recombinant enzyme was selective for isoflavonoid, formononetin and daidzein as substrates, while flavonoids and various tested non-flavonoid compounds were poor substrates.
journal_name
Plantajournal_title
Plantaauthors
Nagashima S,Inagaki R,Kubo A,Hirotani M,Yoshikawa Tdoi
10.1007/s00425-003-1118-0subject
Has Abstractpub_date
2004-01-01 00:00:00pages
456-9issue
3eissn
0032-0935issn
1432-2048journal_volume
218pub_type
杂志文章相关文献
PLANTA文献大全abstract::The bud-inducing effect of the cytokinin N(6)-(Δ(2)-isopentenyl)-adenine (i(6)-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i(6)-Ade, bud-formation was accelerated in both types ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392232
更新日期:1985-08-01 00:00:00
abstract::Sporangiophores of Phycomyces blakesleeanus that are gravitropically stimulated by inclining them relative to the earth's gravitational vector obey the sine law for inclination angles between 0 degrees and 150 degrees. The quantitative relation between gravitropism and phototropism was analyzed for sporangiophores tha...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-001-0705-1
更新日期:2002-04-01 00:00:00
abstract::Efficiency of novel fiber formation was much improved in protoplast culture of embryogenic cells (ECs) of a conifer, Larix leptolepis (Sieb. et Zucc.) Gord., by pre-culturing ECs in a medium containing a high concentration of glutamine (13.7 mM). The fibrillar substructures of large and elongated fibers of protoplasts...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0065-3
更新日期:2005-12-01 00:00:00
abstract:MAIN CONCLUSION:Circular RNAs (circRNAs) identification, expression profiles, and construction of circRNA-parental gene relationships and circRNA-miRNA-mRNA ceRNA networks indicate that circRNAs are involved in flag leaf senescence of rice. Circular RNAs (circRNAs) are a class of 3'-5' head-to-tail covalently closed no...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03544-6
更新日期:2021-01-07 00:00:00
abstract:MAIN CONCLUSION:Endogenous auxin determines the pattern of adventitious shoot formation. Auxin produced in the dominant shoot is transported to the internodal segment and suppresses growth of other shoots. Adventitious shoot formation is required for the propagation of economically important crops and for the regenerat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03367-5
更新日期:2020-03-05 00:00:00
abstract::Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l myoinositol, 0.1 mg/l thiami...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392306
更新日期:1987-08-01 00:00:00
abstract:MAIN CONCLUSION:Exogenously applied 2% fructose is the most appropriate carbon source that enhances photosynthesis and growth of grape plantlets compared with the same concentrations of sucrose and glucose. The role of the sugars was regulated by the expression of key candidate genes related to hormones, key metabolic ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2712-x
更新日期:2017-09-01 00:00:00
abstract::Maize (Zea mays L.) protoplasts obtained from Type I and Type II calli from several genotypes were shown to be capable of synthesizing cell walls and forming small clusters of cells. The medium used also supported cluster formation from protoplasts obtained from root tips. The effects of various additions to the mediu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395067
更新日期:1987-05-01 00:00:00
abstract::The influx of L-[(14)C]valine was measured over a wide concentration range (1 μM to 100 mM) in immature, isolated pea (Pisum sativum L. cv. Marzia) cotyledons at two developmental stages, both in the absence and in the presence of 0.4 M mannitol. At an early developmental stage (water content of the cotyledons ≈80%) t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00193013
更新日期:1990-07-01 00:00:00
abstract::The changes in packed cell volume and in nitrate content and conductivity of the medium during the growth cycle of cell suspension cultures from Petroselinum hortense Hoffm., Glycine max Merr., and Haplopappus gracilis A. Grey in a chemically defined medium were compared. In all three cases sigmoidal curves obtained f...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388694
更新日期:1975-01-01 00:00:00
abstract::To determine the sites of synthesis of chloroplast-envelope proteins, we have analysed several enzyme and translocator functions ascribed to the envelope membranes, and investigated the envelope polypeptide composition of plastids isolated from 70S ribosome-deficient leaves of rye (Secale cereale L.) generated by grow...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391269
更新日期:1985-12-01 00:00:00
abstract::The tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase (EGase) Cel1 protein was characterized in fruit using specific antibodies. Two polypeptides ranging between 51 and 52 kDa were detected in the pericarp, and polypeptides ranging between 49 and 51 kDa were detected in locules. The polypeptides recognize...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1321-7
更新日期:2004-11-01 00:00:00
abstract::Expression of genes in plant chloroplasts provides an opportunity for enhanced production of target proteins. We report the introduction and expression of a fusion DPT protein containing immunoprotective exotoxin epitopes of Corynebacterium diphtheriae, Bordetella pertussis, and Clostridium tetani in tobacco chloropla...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0918-2
更新日期:2009-05-01 00:00:00
abstract::Growth inhibitors were assayed from extracts of intact (attached) and of excised (cultured) root caps of Zea mays L., cv. Merit, the roots of which show a positive geotropic response only after exposure to light. If caps are intact at the time of illumination, at least two inhibitory substances are produced, an acid i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394989
更新日期:1981-12-01 00:00:00
abstract::Individual leaves of eastern cottonwood (Populus deltoides), representing an ontogenetic series from leaf plastochron index 0.0 to 8.0, were fed (14)CO2 photosynthetically and then harvested at times ranging from 15 to 1440 min. The lamina of each fed leaf was sectioned from tip to base into 5 parts, and each part was...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386392
更新日期:1972-12-01 00:00:00
abstract::Aspartate kinase (EC 2.7.2.4.) has been purified from 7 day etiolated wheat (Triticum aestivum L. var. Maris Freeman) seedlings and from embryos imbibed for 8 h. The enzyme was 50% inhibited by 0.25 mM lysine. In this study wheat aspartate kinase was not inhibited by threonine alone or cooperatively with lysine; these...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387136
更新日期:1978-01-01 00:00:00
abstract::Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca(2+) in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397537
更新日期:1983-11-01 00:00:00
abstract::Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0471-1
更新日期:2007-06-01 00:00:00
abstract::Leaf and minor vein structure were studied in Arabidopsis thaliana (L.) Heynh. to gain insight into the mechanism(s) of phloem loading. Vein density (length of veins per unit leaf area) is extremely low. Almost all veins are intimately associated with the mesophyll and are probably involved in loading. In transverse s...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000268
更新日期:2000-06-01 00:00:00
abstract::The role of acidic SK(n) dehydrins in stress tolerance of important crop and model species of the Solanaceae remains unknown. We have previously shown that the acidic SK₃ dehydrin DHN24 from Solanum sogarandinum is constitutively expressed and its expression is associated with cold acclimation. Here we found that DHN2...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-2018-6
更新日期:2014-04-01 00:00:00
abstract::Mature chromoplasts from daffodil (Narcissus pseudonarcissus) flowers, although devoid of thylakoid structures, contain immunologically detectable alpha-subunits of ATP-synthase (H(+)-transporting ATP phosphohydrolase; EC 3.6.3.14). To show the presence of the entire functional protein complex, chromoplast membrane pr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-001-0724-y
更新日期:2002-05-01 00:00:00
abstract::Plants of Pinguicula lusitanica were grown in individual Erlenmeyer flasks on an inorganic agar medium containing no nitrogen or phosphorus. After 8 weeks of culture, twenty of the plants were fed Pinus pollen 4 times over a period of 5 weeks.As a result of the feeding, the number of leaves as well as the diameters of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384859
更新日期:1967-03-01 00:00:00
abstract::The lipid composition and degree of unsaturation of fatty acids has been measured for membranes isolated from leaves of Pisum sativum grown under either summer or winter conditions. Although the lipid-class composition was not significantly changed for the two growth conditions the degree of unsaturation of the fatty ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00405185
更新日期:1983-04-01 00:00:00
abstract::The adaxial leaf cuticle of Malus pumila was examined by electron microscopy to determine possible avenues for transcuticular movement of foliarly applied chemicals. Cutin-embedded polysaccharide microfibrils originated at the outer epidermal cell wall and occasionally extended to the cuticle surface. Lamellae, ca. 4 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388736
更新日期:1979-12-01 00:00:00
abstract::The properties of the water-soluble metabolites of [(3)H]gibberellin A1 ([(3)H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385994
更新日期:1977-01-01 00:00:00
abstract::Cells of Chlamydomonas reinhardtii Dang. wild type and the cell-wall mutants cw2 and cw15 were grown synchronously. The two mutants secreted copious amounts of cell-wall-like glycoproteins into the culture medium in contrast to the wild type which released only minor quantities. Both the secreted proteins as well as t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194001
更新日期:1990-01-01 00:00:00
abstract::Seed germination of Nicotiana tabacum L. cv. Havana 425 is determined by the balance of forces between the growth potential of the embryo and the mechanical restraint of the micropylar endosperm. In contrast to the gibberellin GA4, the brassinosteroid (BR) brassinolide (BL) did not release photodormancy of dark-imbibe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100542
更新日期:2001-09-01 00:00:00
abstract::In leaves of S. nigra, fragmentation of the rachis follows the autumnal abscission of leaflets and the high levels of ethylene produced by the senescing blades. Fragmentation is accompanied by cell growth and ultrastructural changes in a zone of cells precisely differentiated at the separation zone. Studies with expla...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384421
更新日期:1976-01-01 00:00:00
abstract::Two-year old saplings grown from cuttings of Cryptomeria japonica D. Don initiate strobilus development following treatment with gibberellic acid under long-day photoperiods. At 25 degrees C with a 14-h photoperiod in a phytotron, male strobili initiated normally; however, they remained green and fell from the sapling...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-1080-x
更新日期:2003-11-01 00:00:00
abstract:MAIN CONCLUSION:Simultaneous nitrogen depletion and 3,4-dichlorophenol addition induce a bioenergetic microalgal reprogramming, through strong Cyt b 6 f synthesis, that quench excess electrons from dichlorophenol's biodegradation to an overactivated photosynthetic electron flow and H 2 -productivity. Cellular energy ma...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2816-3
更新日期:2018-03-01 00:00:00