Abstract:
:Expression of genes in plant chloroplasts provides an opportunity for enhanced production of target proteins. We report the introduction and expression of a fusion DPT protein containing immunoprotective exotoxin epitopes of Corynebacterium diphtheriae, Bordetella pertussis, and Clostridium tetani in tobacco chloroplasts. Using biolistic-mediated transformation, a plant-optimized synthetic DPT gene was successfully transferred to tobacco plastomes. Putative transplastomic T0 plants were identified by PCR, and Southern blot analysis confirmed homoplasmy in T1 progeny. ELISA assays demonstrated that the DPT protein retained antigenicity of the three components of the fusion protein. The highest level of expression in these transplastomic plants reached 0.8% of total soluble protein. To assess whether the functional recombinant protein expressed in tobacco plants would induce specific antibodies in test animals, a mice feeding experiment was conducted. For mice orally immunized with freeze-dried transplastomic leaves, production of IgG and IgA antibodies specific to each toxin were detected in serum and mucosal tissues.
journal_name
Plantajournal_title
Plantaauthors
Soria-Guerra RE,Alpuche-Solís AG,Rosales-Mendoza S,Moreno-Fierros L,Bendik EM,Martínez-González L,Korban SSdoi
10.1007/s00425-009-0918-2subject
Has Abstractpub_date
2009-05-01 00:00:00pages
1293-302issue
6eissn
0032-0935issn
1432-2048journal_volume
229pub_type
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