Abstract:
:In crude extracts from the primary leaf of wheat seedlings, Triticum aestivum L., cv. Olympic, maximum proteinase activity, as determined by measuring the rate of release of amino nitrogen from ribulose-bisphosphate carboxylase (RuBPCase), was found to be obtained only when EDTA and L-cysteine were included in the extraction buffer. Highest proteinase activity was obtained by grinding at pH 6.8, although the level of activity was similar in the pH range 5.6 to 8.0; this range also coincided with maximum extractability of protein. The lower amount of RuBPCase degrading proteinase extracted at low pH was not due to an effect of pH on enzyme stability. The optimum temperature of reaction was 50° C and reaction rates were linear for at least 120 min at this temperature. In the absence of substrate the proteinase was found to be very sensitive to temperatures above 30° C, with even short exposures causing rapid loss of activity. The relation between assay pH and RuBPCase degradation indicated that degradation was restricted to the acid proteinase group of enzymes, with a pH optimum of 4.8, and no detectable activity at a pH greater than 6.4. The levels of extractable RuBPCase proteinase exhibited a distinct diurnal variation, with activity increasing during the latter part of the light period and then declining once the lights were turned off. The effect of leaf age on the level of RuBPCase, RuBPCase proteinase and total soluble protein was investigated. Maximum RuBPCase activity occurred 9 days after sowing as did soluble protein. After the maximum level was obtained, the pattern of total soluble protein was shown to be characterised by three distinct periods of protein loss: I (day 9-13) 125 ng leaf(-1) day(-1); II (day 15-27) 11 ng leaf(-1) day(-1); III (day 29-49) 22 ng leaf(-1) day(-1). Comparison of the pattern of RuBPCase activity and total protein suggest that the loss of RuBPCase may be largely responsible for the high rate of protein loss during period I. Proteinase activity increased sharply during the period of most rapid loss of RuBPCase activity, and because the specific activity of RuBPCase also declined, we concluded that RuBPCase was being degraded more rapidly than the other proteins. Once the majority of the RuBPCase was lost, there did not appear to be a direct relation between RuBPCase proteinase activity and rate of total soluble protein loss, since the proteinase exhibited maximum activity during the slowest period of protein loss (II), and was declining in activity while the rate of protein loss remained stable during the third and final period of total protein loss.
journal_name
Plantajournal_title
Plantaauthors
Peoples MB,Dalling MJdoi
10.1007/BF00391172subject
Has Abstractpub_date
1978-01-01 00:00:00pages
153-60issue
2eissn
0032-0935issn
1432-2048journal_volume
138pub_type
杂志文章相关文献
PLANTA文献大全abstract:MAIN CONCLUSION:Phloem production and structural development were interlinked with seasonal variation in the primary and secondary metabolites of phloem. Novel microtechniques provided new perspectives on understanding phloem structure and chemistry. To gain new insights into phloem formation in Norway spruce (Picea ab...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2347-8
更新日期:2015-09-01 00:00:00
abstract::Cycloheximide (CH) inhibited the division of generative cell in pollen cultures of Impatiens balsamina in concentrations of 1-200 μg/ml without affecting pollen germination or pollen tube growth. This inhibition was reversible up to 3 h in hanging drop cultures and up to 6 h in test tube cultures by replacement of the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390799
更新日期:1974-06-01 00:00:00
abstract::Two types of auxin-binding sites (sites I and II) in membranes from maize (Zea mays L.) coleoptiles were characterized. Site I was a protein with a relative molecular mass of 21 000, and the distribution of site I protein on sucrose density gradient fractionation coincided with that of NADH-cytochrome-c reductase (EC ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393079
更新日期:1988-10-01 00:00:00
abstract::Fine-structural investigations of 24 monocotyledons from 21 families and all but one order succeeded in revealing a plastid with cuneate proteinaceous inclusion bodies as being typical of monocot sieve-tubes. Inclusion bodies originate in large numbers during plastid differentiation; they concentrate in the matrix and...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398395
更新日期:1968-06-01 00:00:00
abstract::The role of purine and pyrimidine ribosides, nucleotides and substituted xanthines in the differentiation of chloronema filaments in suspension cultures of protonema of the moss Funaria hygrometrica Hedw. has been examined. Cyclic adenosine-3',5'-monophosphate (cAMP) and mono-and dibutyryl cAMP evoked the maximum resp...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391574
更新日期:1979-01-01 00:00:00
abstract::Plants are exposed to a wide range of toxic and bioactive low-molecular-weight molecules from both exogenous and endogenous sources. Glycosylation is one of the primary sedative mechanisms that plants utilise in order to maintain metabolic homeostasis. Recently, a range of glycosyltransferases has been characterized i...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s004250000492
更新日期:2001-06-01 00:00:00
abstract:MAIN CONCLUSION:Recent investigations have provided important new insights into the structures and functions of the extrinsic proteins of Photosystem II. This review is an update of the last major review on the extrinsic proteins of Photosystem II (Bricker et al., Biochemistry 31:4623-4628 2012). In this report, we wil...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-015-2462-6
更新日期:2016-04-01 00:00:00
abstract::The time-response of the CO2-exchange of both leaf surfaces was measured separately. Leaves of Primula palinuri and Zea mays were used for the study. After short dark-periods (3 min) the stomata are not closed. Consequently CO2-uptake starts quickly after re-illumination and reaches the steady-state value very rapidly...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386495
更新日期:1969-03-01 00:00:00
abstract::Five major soluble nuclear proteins associated with cell proliferation were identified in Allium cepa L. root cells. One of them, of 64 kDa, was revealed by Western blotting with anti-mammalian nucleolin antibodies. A polyclonal antibody raised against this protein, which we have named NopA64, localised it in the nucl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050093
更新日期:1997-01-01 00:00:00
abstract::Glycosyltransferase complexes are known to be involved in plant cell wall biosynthesis, as for example in cellulose. It is not known to what extent such complexes are involved in biosynthesis of pectin as well. To address this question, work was initiated on ARAD1 (ARABINAN DEFICIENT 1) and its close homolog ARAD2 of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1592-3
更新日期:2012-07-01 00:00:00
abstract::The permeability of biological membranes to boric acid was investigated using the giant internodal cells of the charophyte alga Chara corallina (Klein ex Will. Esk. R.D. Wood). The advantage of this system is that it is possible to distinguish between membrane transport of boron (B) and complexing of B by plant cell w...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000484
更新日期:2001-05-01 00:00:00
abstract::P34(cdc2) is a key cell-cycle protein in fission yeast that is necessary for progress in the cell cycle from the G1 to the S phase and from G2 through mitosis. Homologues of p34(cdc2) have been found in all eukaryotes that have been investigated. Levels of p34(cdc2)-like protein were studied by quantitative Western bl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201048
更新日期:1991-10-01 00:00:00
abstract:MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03435-w
更新日期:2020-08-01 00:00:00
abstract::In order to understand the mode of action of auxins and cytokinins in the induction of cell division, the effects of the two plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzyladenine (BA) were investigated using mesophyll protoplasts of Petunia hybrida, cultivated in either complete medium or...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050393
更新日期:1998-10-01 00:00:00
abstract:MAIN CONCLUSION:Xyloglucan endo-transglycosylase/hydrolase ( Ph XET/H) regulates Podophyllum seed germination via GA mediated up-accumulation of Ph XET protein and subsequent endosperm weakening. Xyloglucan endo-transglycosylase/hydrolase (XET/H) belong to glycosyl hydrolase family 16, which play an important role in e...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2520-8
更新日期:2016-08-01 00:00:00
abstract::Electrophysiological experiments have indicated that a fraction of the major auxin-binding protein (ABP1) of maize (Zea mays L.) might be a receptor on the outer surface of the plasma membrane. The predominant location of ABP1 is in the lumen of the endoplasmic reticulum (ER), in accord with its C-terminal KDEL retent...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00196668
更新日期:1995-01-01 00:00:00
abstract::Cells of adenine(Ad1)- and pantothenate(Pn1)-requiring cells lines ofDatura innoxia Mill. were killed by 2 mM arsenate in the medium when growing but were not killed when in a non-growing state which was induced by starvation for required metabolites. This observation confirmed that arsenate might be useful as a count...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00998808
更新日期:1983-01-01 00:00:00
abstract::Water potential, osmotic potential and turgor measurements obtained by using a cell pressure probe together with a nanoliter osmometer were compared with measurements obtained with an isopiestic psychrometer. Both types of measurements were conducted in the mature region of Tradescantia virginiana L. leaves under non-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392152
更新日期:1989-01-01 00:00:00
abstract::Quinate: NAD(+) oxidoreductase (EC 1.1.1.24) from carrot cells was deactivated by incubating partially purified extract with MgCl2 at 30°C. The deactivation process was prevented by adding fluoride, a phosphatase inhibitor. Once inactivated, the enzyme could recover its initial activity on incubation with ATP-Mg eithe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387863
更新日期:1982-05-01 00:00:00
abstract::The influence of nucleoside triphosphates in relation to divalent cations on RNA synthesis of cells from a suspension culture from parsley was investigated. The data obtained from experiments with isolated nuclei and with an in vitro system with highly purified RNA polymerase I were compared with a chromatin-bound nuc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385363
更新日期:1981-08-01 00:00:00
abstract::The symbiosis between legume plants and rhizobia causes the development of new organs, nodules which function as an apparatus for nitrogen fixation. In this study, the roles of auxin in nodule development in Lotus japonicus have been demonstrated using molecular genetic tools and auxin inhibitors. The expression of an...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1385-0
更新日期:2011-07-01 00:00:00
abstract::Phytochrome was found by direct spectrophotometry to be present in whole dry seeds of cucumber. This pigment is spectroscopically different from the pigment found in etiolated plants. It shows the phenomenon of inverse reversion; in darkness, the red-absorbing form (Pr) reverts slowly to the far-red-absorbing form (Pf...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387458
更新日期:1969-12-01 00:00:00
abstract::Secoisolariciresinol diglucoside (SDG), the main phytoestrogenic lignan of Linum usitatissimum, is accumulated in the seed coat of flax during its development and pinoresinol-lariciresinol reductase (PLR) is a key enzyme in flax for its synthesis. The promoter of LuPLR1, a flax gene encoding a pinoresinol lariciresino...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1492-y
更新日期:2012-01-01 00:00:00
abstract::The role of oxygen in the photoinactivation of the photosynthetic apparatus of Spinacia oleracea L. was investigated. Moderate irradiation (1200 μmol photons m(-2)s(-1)) of spinach leaves in an atmosphere of pure nitrogen caused strong inhibition of subsequently measured net CO2 assimilation, whereas considerably less...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392242
更新日期:1985-08-01 00:00:00
abstract::The qualitative and quantitative composition of the carotenoids of young cells of Euglena gracilis strain 1224-5/9 corresponded to that of the "Z"-strain.The frequently observed yellow-reddish discolouration of ageing cells was found to be caused by a heavy breakdown of the chlorophylls and not by an increased synthes...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387621
更新日期:1971-06-01 00:00:00
abstract::We have investigated events which take place in the developing barley (Hordeum vulgare L.) embryo during its acquisition of desiccation tolerance. Excised embryos are capable of precocious germination as early as 8 d after pollination (DAP). At this age, however, they are not capable of resisting a desiccation treatme...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393069
更新日期:1988-10-01 00:00:00
abstract::Elongating upper and non-elongating lower segments of mung bean hypocotyls (Phaseolus aureus Roxb.) were compared in their ability to incorporate labelled polymers into their cell walls when fed with [(14)C]proline. The non-growing segments incorporated more amino acids into the walls and showed a higher degree of hyd...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00429047
更新日期:1974-09-01 00:00:00
abstract::From the initial discovery of free-running endogenous circadian oscillations of Crassulacean acid metabolism (CAM) under constant conditions in the light and in air, it has been disputed whether the underlying oscillator is enzymic or biophysical. The hypothesis of a biophysical hysteresis switch or beat oscillator st...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s004250000408
更新日期:2000-11-01 00:00:00
abstract::Cadmium contamination is a serious environmental problem for modern agriculture and human health. Salinity affects plant growth and development, and interactions between salt and cadmium have been reported. However, the molecular mechanisms of salinity-cadmium interactions are not fully understood. Here, we show that ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-1070-8
更新日期:2010-01-01 00:00:00
abstract::The nitric oxide (NO) donor sodium nitroprusside (SNP) significantly promoted germination of switchgrass (Panicum virgatum L. cv Kanlow) in the light and in the dark at 25 degrees C, across a broad range of concentrations. SNP also promoted seed germination in two other warm-season grasses. A chemical scavenger of NO ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0162-3
更新日期:2006-05-01 00:00:00