Abstract:
:P34(cdc2) is a key cell-cycle protein in fission yeast that is necessary for progress in the cell cycle from the G1 to the S phase and from G2 through mitosis. Homologues of p34(cdc2) have been found in all eukaryotes that have been investigated. Levels of p34(cdc2)-like protein were studied by quantitative Western blotting in developing cotyledons of Daucus carota L. (carrot) seedlings, in expiants from the same seedlings transferred to tissueculture media with and without 2,4-dichlorophenoxyacetic acid (2,4-D), and in nutrient-starved suspension cultures derived from carrot callus. During the cessation of cell division, which accompanies development of the cotyledon to maturity, there was a 16-fold decline in the level of the p34(cdc2)-like protein. Auxin-stimulated dedifferentiation in excised tissue from mature cotyledons was accompanied by restoration of the level of p34(cdc2)-like protein, and the responding cells formed a callus. These data support our earlier proposition, based upon evidence from wheat leaf, that changes in the level of p34(cdc2)-like protein act in the switch between cycling and differentiation. Persisting high levels of p34(cdc2)-like protein in suspension cultures, when division was stopped by nutrient limitation, indicated that decline of the protein was not an inevitable consequence of the cessation of division. Decline of p34(cdc2) in differentiation may therefore be a regulated process that determines exit from the cell cycle and the converse increase in p34(cdc2) may be a regulated process controlling dedifferentiation and resumption of cell division.
journal_name
Plantajournal_title
Plantaauthors
Gorst JR,John PC,Sek FJdoi
10.1007/BF00201048subject
Has Abstractpub_date
1991-10-01 00:00:00pages
304-10issue
3eissn
0032-0935issn
1432-2048journal_volume
185pub_type
杂志文章相关文献
PLANTA文献大全abstract::The effects of the local anesthetic dibucaine on coupling between electron transport and ATP synthesis-hydrolysis by the coupling-factor complex (CF0CF1 ATPase) were investigated in thylakoid membranes from Spinacia oleracea L. cv. Monatol. Evidence is presented that inhibition of ATP synthesis was produced by a speci...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194278
更新日期:1991-03-01 00:00:00
abstract::The (12)CO2- and (14)CO2-exchange of illuminated corn leaf discs were measured at normal (21%) and low (1%) oxygen. After periods of exposure to (14)CO2 or to (14)CO2 followed by (12)CO2, the discs were killed and the specific activities of some metabolites were determined. At both O2 concentrations the specific activ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384921
更新日期:1974-01-01 00:00:00
abstract::We studied changes induced by cold on electron transfer pathways (linked to NADH or NADPH oxidation) in endoplasmic reticulum of rapeseed hypocotyls (Brassica napus L.) from a freezing-sensitive variety (ISL) and freezing-tolerant variety (Tradition). Plantlets were grown at 22 degrees C then submitted to a cold shock...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0793-2
更新日期:2008-10-01 00:00:00
abstract::Heterocysts of Anabaena cylindrica, isolated rapidly in the cold, were found-in contrast to earlier reports-to contain all of the same lipids and lipophilic pigments, and in about the same proportions, as vegetative cells. In broken filaments and in heterocysts damaged during isolation, the membrane lipids and certain...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398015
更新日期:1972-03-01 00:00:00
abstract::Three-week-old protonemata of Funaria hygrometrica Hedw. cultivated in Petri dishes tolerate slow drying (24 h to complete dryness) but not rapid drying (1h to complete dryness). Slowly dried mosses show, on a dry-weight basis, a sixfold increase in abscisic-acid (ABA) contents during the drying process. Rehydrated, s...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201503
更新日期:1991-12-01 00:00:00
abstract::Seven endochitinases (EC 3.2.1.14) (relative molecular masses 23,000-28,000 and isoelectric points 10.3-10.4) were purified from nonembryogenic Citrus sinensis L. Osbeck cv. Valencia callus tissue. The basic chitinase/lysozyme from this tissue (BCLVC) exhibited lysozyme, chitinase and chitosanase activities and was de...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00200295
更新日期:1996-01-01 00:00:00
abstract::Physical clustering of genes has been shown in plants; however, little is known about gene clusters that have different functions, particularly those expressed in the tomato fruit. A class I 17.6 small heat shock protein (Sl17.6 shsp) gene was cloned and used as a probe to screen a tomato (Solanum lycopersicum) genomi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1518-5
更新日期:2012-03-01 00:00:00
abstract::'Warm white' fluorescent light inhibited the elongation of potato sprouts and reduced the rate at which reserve materials in the mother tuber were mobilized. The evidence indicated that the influence of light on mobilization was independent of light effects on the magnitude of the 'sink' for translocates in the sprout...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385292
更新日期:1967-09-01 00:00:00
abstract::Crude chromatin preparations from pea seedlings contain calcium-dependent deoxyribonuclease activity, at least part of which is endonucleolytic. During germination, there is a dramatic increase in chromatin-bound deoxyribonuclease activity in the embryonic axis immediately prior to the onset of DNA replication. Eviden...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392923
更新日期:1978-01-01 00:00:00
abstract::Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00411141
更新日期:1984-05-01 00:00:00
abstract::The activities of nitrate reductase (EC1.6.6.1), nitrite reductase (EC 1.6.6.4), glutamine synthetase (EC6.3.1.2), glutamate synthase (EC1.4.7.1) and NAD(P)H-dependent glutamate dehydrogenase (EC 1.4.1.3) were investigated in mesophyll and bundle sheath cells of maize leaves (Zea mays L.). Whereas nitrate and nitrite ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384971
更新日期:1977-01-01 00:00:00
abstract::Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000....
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384657
更新日期:1980-12-01 00:00:00
abstract::Two lectins were purified by affinity chromatography from mature peanut (Arachis hypogaea L.) nodules, and compared with the previously characterised seed lectin of this plant. One of the nodule lectins was similar to the seed lectin in its molecular weight and amino-acid composition and ability to bind derivatives of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392475
更新日期:1988-11-01 00:00:00
abstract::Chlamydomonas reinhardtii Dangeard generates photoreceptor currents (PCs) upon light excitation. These currents play a key role in the signal transduction chain for photomotility responses. We have previously found that inhibition of PCs by tryptone occurs only in gametes that display chemotaxis toward this agent, and...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0357-2
更新日期:2007-01-01 00:00:00
abstract::A study has been made of the wall fibrils produced by tobacco protoplasts, using scanning electron microscopy in conjunction with negative staining. It has been shown that the fibres seen in scanning electron microscopy correspond to aggregates of microfibrils. These aggregates are only visible where they are lifted c...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388233
更新日期:1979-01-01 00:00:00
abstract::Seedlings from the white mustard, Sinapis alba, grown under continuous far-red light exhibit enhanced plastid enzyme activities when compared with dark-grown seedlings (for review, see Mohr 1981). These activities are even more pronounced upon illumination with white light during the etioplast/chloroplast transformati...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398686
更新日期:1987-07-01 00:00:00
abstract::Photoheterotrophic and photoautotrophic cell suspension cultures were raised from a callus tissue derived from a Morinda lucida Benth. plant (Rubiaceae). The cultures were characterized with regard to fresh weight, dry weight, cell number, pH, chlorophyll and quinoid natural products. The amount of lipoquinones (phyll...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391279
更新日期:1985-12-01 00:00:00
abstract::In maize and other grasses there is a developmental gradient from the meristematic cells at the base of the stalk to the differentiated cells at the leaf tip. This gradient presents an opportunity to investigate changes in mitochondrial DNA (mtDNA) that accompany growth under light and dark conditions, as done previou...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1802-z
更新日期:2013-02-01 00:00:00
abstract::In the initial phase of the geotropical reaction of the Chara rhizoid the growth difference postulated by Sievers (1967c) between the physically upper, slightly subapical flank and the lower one is demonstrated. In horizontal exposure the growth of the extreme cell apex is continued, while the growth of the lower flan...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386948
更新日期:1971-12-01 00:00:00
abstract::Transgenic rice plants overexpressing a rice tyrosine decarboxylase (TyDC) exhibited a dwarf phenotype with a high level of tyramine accumulation. The height of transgenic rice was reduced on average to 35% of the wild-type height, whereas the number of tillers increased to 190% that of wild type. When judged by cellu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-010-1303-x
更新日期:2011-02-01 00:00:00
abstract::Anthocyanins are the major pigments responsible for the pomegranate (Punica granatum L.) fruit skin color. The high variability in fruit external color in pomegranate cultivars reflects variations in anthocyanin composition. To identify genes involved in the regulation of anthocyanin biosynthesis pathway in the pomegr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1438-4
更新日期:2011-11-01 00:00:00
abstract::MicroRNAs (miRNAs) are emerging as essential regulators of biological processes. Somatic embryogenesis is one of the most important techniques for gymnosperm-breeding programs, but there is little understanding of its underlying mechanism. To investigate the roles of miRNAs during somatic embryogenesis in larch, we co...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1643-9
更新日期:2012-08-01 00:00:00
abstract:MAIN CONCLUSION:Three novel QTLs for grain shape were genetically fine mapped, with two of which to a 250-kb target interval on rice chromosome 2 that contains fourteen candidate genes. Grain shape (grain length, width, and thickness) determines crop yield and grain quality. However, the trait is regulated by numerous ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03420-3
更新日期:2020-07-15 00:00:00
abstract::Nuclear DNA (ncDNA) synthesis in Chlamydomonas reinhardtii was measured by both (32)P[or-thophosphoric acid] ((32)P) and [(14)C]adenine incorporation and found to be highly synchronous. Ca. 85% of incorporation was confined to the first 6 h of the dark period of a synchronized regime consisting of an alternating light...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388341
更新日期:1978-01-01 00:00:00
abstract::Hydrogen peroxide-dependent glyoxylate decarboxylation occurring during glycollate oxidation by pea leaf extracts (Pisum sativum L.) has been studied in relation to the effects of glyoxylate and extract concentration. With a saturating concentration of glycollate, decarboxylation was greatly stimulated by raising the ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392719
更新日期:1982-08-01 00:00:00
abstract::Rehydration of desiccated Cuphea seeds results in a rapid morphological change in the seed. Within 20 min thread like epidermal hairs are present on the seed surface. The hairs, which are highly ordered helical structures, are present in the epidermal cells of the desiccated seed. Following emergence the hairs increas...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394467
更新日期:1982-09-01 00:00:00
abstract::The arrangements of cortical microtubules (MTs) in a tip-growing protonemal cell of Adiantum capillus-veneris L. and of cellulose microfibrils (MFs) in its wall were examined during blue-light (BL)-induced apical swelling. In most protonemal cells which had been growing in the longitudinal direction under red light, a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391861
更新日期:1989-06-01 00:00:00
abstract::Wild-type (Ph1) and adenine-requiring (Ad1) cell lines of Datura innoxia Mill. were used in experiments to evaluate arsenate as a growth-lethal compound and its use as a counterselection agent. These experiments led to the devising of methods for the recovery of Ph1 and Ad1 cells after arsenate treatment when plated a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392866
更新日期:1984-02-01 00:00:00
abstract::It has been shown that the scuttellum of germinating barley embryos synthesises gibberellin in intact grains or when detached from the endosperm, but not when attached to the endosperm if the axis is removed. Evidence is given to support the hypothesis that the inhibition of gibberellin synthesis is caused by a distur...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386454
更新日期:1969-09-01 00:00:00
abstract::Etioplasts and chloroplasts isolated from Pisum sativum differ in lipid composition. The relative amounts of galactolipids, especially of MGD, are higher in the chloroplast. In contrast, the sulfo- and the phospholipids are already present in the etioplasts in higher concentrations. During the formation of the thylako...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386274
更新日期:1973-06-01 00:00:00