Changes in photoreceptor currents and their sensitivity to the chemoeffector tryptone during gamete mating in Chlamydomonas reinhardtii.

Abstract:

:Chlamydomonas reinhardtii Dangeard generates photoreceptor currents (PCs) upon light excitation. These currents play a key role in the signal transduction chain for photomotility responses. We have previously found that inhibition of PCs by tryptone occurs only in gametes that display chemotaxis toward this agent, and is not observed in chemotactically insensitive vegetative cells. Here we show that the sensitivity to tryptone is characteristic of gametes of both mating types, and examine the influence of gamete mating on PCs and their sensitivity to tryptone. The amplitude of PCs increases after cell fusion, but the sensitivity of these currents to tryptone decreases upon flagellar adhesion and/or an increase in the intracellular cAMP concentration. Net chemotaxis toward tryptone is reduced in young zygotes compared to gametes. We conclude that gamete mating leads to rapid inactivation of a gamete-specific chemosensory system.

journal_name

Planta

journal_title

Planta

authors

Govorunova EG,Voytsekh OO,Sineshchekov OA

doi

10.1007/s00425-006-0357-2

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

441-9

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

225

pub_type

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