Abstract：
:Supernatant and resuspended pellet samples from a centrifugation of homogenised, etiolated oat seedlings were prepared and assayed spectrophotometrically for phytochrome in the presence and absence of added calcium carbonate (CaCO3) particles under a variety of conditions. At a constant sample thickness, in the absence of CaCO3, increasing sample concentration had no significant effect on the expected phytochrome reading. In the presence of CaCO3, however, as sample concentration increased, the phytochrome reading was less than, expected more so in resuspended pellet samples than in supernatant samples. At a constant sample concentration in the absence of CaCO3, increasing sample thickness gave no significant difference from the excepted phytochrome reading in supernatant samples, but led to a slight increase over the expected phytochrome reading in resuspended pellet samples. In the presence of CaCO3, increasing sample thickness led to a drop from the expected phytochrome reading in both sample types, but more so in resuspended pellet samples. These findings show that the use of CaCO3 as an aid to spectrophotometric phytochrome assay can lead to large artifacts in the instrument reading and that its use should be approached with caution.
journal_name
Plantajournal_title
Plantaauthors
Watson PJ,Smith Hdoi
10.1007/BF00387903subject
Has Abstractpub_date
1982-03-01 00:00:00pages
115-20issue
2eissn
0032-0935issn
1432-2048journal_volume
154pub_type
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