Abstract：
:A polyclonal antibody has been raised against ferulic acid ester linked to arabinoxylans (AX). 5-O-feruloyl-alpha-L-arabinofuranosyl(1-->4)-beta-D-xylopyranosyl was obtained by chemical synthesis, and was coupled to bovine serum albumin for the immunization of rabbit. The polyclonal antibody designated 5-O-Fer-Ara was highly specific for 5-O-(trans-feruloyl)-L-arabinose (5-O-Fer-Ara) structure that is a structural feature of cell wall AX of plants belonging to the family of Gramineae. The antibody has been used to study the location and deposition of feruloylated AX in walls of aleurone and starchy endosperm of wheat grain. 5-O-Fer-Ara began to accumulate early in aleurone cell wall development (beginning of grain filling, 13 days after anthesis, DAA) and continued to accumulate until the aleurone cells were firmly fixed between the starchy endosperm and the nucelus epidermis (19 DAA). From 26 DAA to maturity, the aleurone cell walls changed little in appearance. The concentration of 5-O-Fer-Ara is high in both peri- and anticlinal aleurone cell walls with the highest accumulation of 5-O-Fer-Ara at the cell junctions at the seed coat interface. The situation is quite different in the starchy endosperm: whatever the stage of development, a low amount of 5-O-Fer-Ara epitope was detected. Contrary to what was observed for aleurone cell walls, no peak of accumulation of feruloylated AX was noticed between 13 and 19 DAA. Visualization of labelled Golgi vesicles suggested that the feruloylation of AX is intracellular. The distribution of (5-O-Fer-Ara) epitope is further discussed in relation to the role of ferulic acid and its dehydrodimers in cell wall structure and tissue organization of wheat grain.
journal_name
Plantajournal_title
Plantaauthors
Philippe S,Tranquet O,Utille JP,Saulnier L,Guillon Fdoi
10.1007/s00425-006-0422-xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
1287-99issue
5eissn
0032-0935issn
1432-2048journal_volume
225pub_type
杂志文章相关文献
PLANTA文献大全abstract：:The profile of (14)C, that is the plot of log (radioactivity) against distance, down the stem of Helianthus seedlings was studied over the course of the first hour after feeding (14)C to a leaf. These seedlings are shown to accumulate a large proportion of the total (14)C present in the stem in an ethanol insoluble fo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385091
更新日期：1970-09-01 00:00:00
abstract：:Light-induced absorbance changes (LIACs), which are associated with early photochemical events of blue-light transduction, were detected in growing zones of Phycomyces sporangiophores. The novel LIACs meet all the essential requirements for a spectrophotometric photoreceptor assay which was previously unavailable for ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050559
更新日期：1999-04-01 00:00:00
abstract：:An endo-beta-1,4-glucanase (EG) was purified from ripe strawberry (Fragaria x ananassa Duch.) fruit using cellulose affinity chromatography. The purified enzyme gave a single protein band of 54 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of this protein showed ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250100577
更新日期：2001-11-01 00:00:00
abstract：:Analogies and differences between the growth-promoting action of fusicoccin (FC), the toxic agent produced by Fusicoccum amygdali, and of indole-3-acetic acid (IAA) in etiolated pea internode segments were further investigated. It was confirmed that the effect of FC, at optimal concentration, on growth by cell enlarge...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387059
更新日期：1973-12-01 00:00:00
abstract：:Ascorbic acid accumulation in the mustard seedling is controlled by P fr(active phytochrome). Kinetic studies demonstrate that P frexerts a rapid and fully reversible control over the steady state rate of ascorbic acid accumulation. Following the terminology of Weisz (1967) for this type of metabolic control the term ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387122
更新日期：1970-06-01 00:00:00
abstract：:Pressure-probe measurements and single-cell sampling and analysis techniques were used to determine the effect of photosynthetic production and accumulation of sugars on osmotic and turgor pressures of individual cells of barley ( Hordeum vulgare L.) source leaves. In control plants, the changes in osmotic pressure in...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0744-2
更新日期：2002-06-01 00:00:00
abstract：:The development of maximal rates of lipid and protein hydrolysis in cucumber cotyledons depends upon the removal of the testa and the presence of the embryonic axis. The testa appears to exert at least part of its inhibitory influence by suppressing the development of enzyme activity associated with lipolysis and prot...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388835
更新日期：1979-10-01 00:00:00
abstract：:Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00454448
更新日期：1979-01-01 00:00:00
abstract：MAIN CONCLUSION:The physiological phenotype of potato tubers afflicted by zebra chip disease is characterized by increased oxidative stress metabolism and upregulation of systems for its mitigation. Starch catabolism and extensive buildup of reducing sugars render potatoes infected with zebra chip (ZC) pathogen (Candid...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-017-2714-8
更新日期：2017-10-01 00:00:00
abstract：:D-threo chloramphenicol (CAP) at 5×10(-5) M, given continuously during a 24-hr aging period and subsequent post-age treatment with 2,4-dichlorophenoxyacetic acid (2,4-D)±kinetin markedly depressed cell expansion in Jerusalem artichoke (Helianthus tuberosus) tuber slices. Both the rate and total amount of expansion wer...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00431120
更新日期：1970-03-01 00:00:00
abstract：:We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Delta166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Delta166 predominantly phosphorylates AtMEK1, an A...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-005-0126-7
更新日期：2006-03-01 00:00:00
abstract：:Responses of plant tissue to environmental challenges can vary among different plant parts and among plants of different ages. Investment into defense has been proposed to be influenced by fitness value and/or allocation of available resources. Here we show at first time at transcriptome level that plant defense is no...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-008-0777-2
更新日期：2008-10-01 00:00:00
abstract：:Primary roots of Lepidium sativum (L.s.) grew upwards in glass capillaries in order to prevent mechanically the geotropic curvature. The central cap cells (statocytes) were investigated with regard to the polar arrangement of their organelles. After 20 minutes of inversion, which correspond to the latent period of L.s...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388858
更新日期：1975-01-01 00:00:00
abstract：:It is concluded from (14)C labelling kinetics as well as from enzyme analysis that formation of malate and aspartate from glycerate-3P via carboxylation of P-enolpyruvate occurs in isolated photosynthesizing spinach (Spinacia oleracea L.) chloroplasts. Carbon incorporation into both dicarboxylic acids was about 1% of ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388873
更新日期：1975-01-01 00:00:00
abstract：:A crude organelle preparation from germinating castor bean endosperm catalysed the incorporation of galactose from UDP[(14)C]galactose into chloroform/methanol (2:1)-soluble glactolipids. At least two galactolipids were formed. Most of the [(14)C]galactose was present in a galactolipid synthesized by the microsomal me...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384596
更新日期：1979-10-01 00:00:00
abstract：:A 21-kDa calmodulin (CaM)-binding protein and a 19-kDa calmodulin-binding protein were detected in 0.1 M CaCl2 extracts of Angelica dahurica L. suspension-cultured cells and carrot (Daucus carota L.) suspension-cultured cells, respectively, using a biotinylated cauliflower CaM gel-overlay technique in the presence of ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00262636
更新日期：1996-04-01 00:00:00
abstract：:The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic fo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385321
更新日期：1981-10-01 00:00:00
abstract：:1. The plastids of the saprophytic orchid Neottia nidus-avis contain, besides chlorophyll a, all plastidquinones and carotenoids of normal chloroplasts from higher plants. The relative concentrations of plastidquinones and carotenoids are, however, quite different from those in photosynthetically active plant tissue. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388961
更新日期：1969-12-01 00:00:00
abstract：:Synthesis of anthocyanin pigments in etiolated cabbage seedlings is influenced by ethylene at concentrations higher than 10 ppb, and etiolated seedlings produce sufficient ethylene to influence their anthocyanin synthesis. When escape of endogenous ethylene from this tissue is enhanced by means of hypobaric treatment,...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387635
更新日期：1973-09-01 00:00:00
abstract：:Sphagnum magellanicum shows a remarkable variation in colour after the repeated application of cold shocks; mainly red wall pigments are synthesized. An attempt was made to find out in which way the gas exchange of Sphagnum magellanicum under defined conditions is altered after the application of cold shocks.The light...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388819
更新日期：1968-03-01 00:00:00
abstract：:Seedlings of Amaranthus caudatus L. var. Pendula were used to study the influence of several treatment: white light, 3,4-dihydroxyphenylalanine (DOPA), kinetin, gibberellic acid (GA3) on betalains biosynthesis. The pigments, betacyanins and betaxanthins, were separated using a Sephadex G-15 column chromatography. Qual...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00380880
更新日期：1980-07-01 00:00:00
abstract：:Several phosphate transporters (PTs) that belong to the Pht2 family have been released in bioinformatics databases, but only a few members of this family have been functionally characterized. In this study, we found that wheat TaPHT2;1 shared high identity with a subset of Pht2 in diverse plants. Expression analysis r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-012-1836-2
更新日期：2013-04-01 00:00:00
abstract：:Ozone (O3), a major photochemical oxidant, causes leaf injury in plants. Plants synthesize salicylic acid (SA), which is reported to greatly affect O3 sensitivity. However, the mechanism of SA biosynthesis under O3 exposure remains unclear. Plants synthesize SA either by a pathway involving phenylalanine as a substrat...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0556-5
更新日期：2007-10-01 00:00:00
abstract：:Plants of Pinguicula lusitanica were grown in individual Erlenmeyer flasks on an inorganic agar medium containing no nitrogen or phosphorus. After 8 weeks of culture, twenty of the plants were fed Pinus pollen 4 times over a period of 5 weeks.As a result of the feeding, the number of leaves as well as the diameters of...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384859
更新日期：1967-03-01 00:00:00
abstract：MAIN CONCLUSION:Site-specific changes of photosynthesis, a relatively new concept, can be used to improve the productivity of critical food crops to mitigate the foreseen food crisis. Global food security is threatened by an increasing population and the effects of climate change. Large yield improvements were achieved...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-019-03223-1
更新日期：2019-10-01 00:00:00
abstract：:In this work, we analyze protein phosphatase (PP) involvement in the sucrose-mediated induction of fructan metabolism in wheat (Triticum aestivum). The addition of okadaic acid (OA), a PP-inhibitor, to sucrose-fed leaves reduced fructosylsucrose-synthesizing activity (FSS) induction in a dose-dependent manner. The exp...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-1002-7
更新日期：2009-10-01 00:00:00
abstract：:Eukaryotic genomes harbor mobile genetic elements known as long terminal repeat (LTR) retrotransposons. LTR retrotransposons are closely related to the infectious and endogenous retroviruses. The viral envelope (env) gene of the retroviruses, which is responsible for their infective properties, distinguishes them from...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0903-5
更新日期：2002-12-01 00:00:00
abstract：MAIN CONCLUSION:Overexpressing a heat shock factor gene (TaHsfA6bT) from wheat provides thermotolerance in barley by constitutive expression of heat and other abiotic stress-response genes. Temperature is one of the most crucial abiotic factors defining the yield potential of temperate cereal crops, such as barley. The...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-020-03457-4
更新日期：2020-09-18 00:00:00
abstract：:Individual leaves of eastern cottonwood (Populus deltoides), representing an ontogenetic series from leaf plastochron index 0.0 to 8.0, were fed (14)CO2 photosynthetically and then harvested at times ranging from 15 to 1440 min. The lamina of each fed leaf was sectioned from tip to base into 5 parts, and each part was...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386392
更新日期：1972-12-01 00:00:00
abstract：:The ability to non-invasively measure metabolic oxygen flux is a very important tool for physiologists interested in a variety of questions ranging from basic metabolism, growth/development, and stress adaptation. Technologies for measuring oxygen concentration near the surface of cells/tissues include electrochemical...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-010-1234-6
更新日期：2010-10-01 00:00:00
