Inhibition of the transthylakoid gradient of electrochemical proton potential by the local anesthetic dibucaine.

Abstract:

:The effects of the local anesthetic dibucaine on coupling between electron transport and ATP synthesis-hydrolysis by the coupling-factor complex (CF0CF1 ATPase) were investigated in thylakoid membranes from Spinacia oleracea L. cv. Monatol. Evidence is presented that inhibition of ATP synthesis was produced by a specific uncoupling mechanism which was based on dibucaine-membrane surface interactions rather than on the interaction of dibucaine with the ATPase complex. Dibucaine reduced the osmotic space of thylakoid vesicles. At low pH of the medium it stimulated ATP hydrolysis beyond the rates obtained with optimum concentrations of 'classical' uncouplers. After addition of dibucaine, there was displacement of membrane-bound Mg(2+) and strong thylakoid stacking in the presence of only low Mg(2+) concentrations. Inhibition of ATP synthesis and transmembrane pH gradient increased with medium pH. Hydrolysis of ATP by isolated CF1 and the CF0CF1 complex was only slightly affected by dibucaine. The data are discussed assuming the involvement of localized proton channels on the membrane surface in protonic coupling of electron transport and ATP synthesis. A hypothesis for the mechanisms of action of local anesthetics at the thylakoid membrane is presented.

journal_name

Planta

journal_title

Planta

authors

Laasch H,Schumann J,Günther G

doi

10.1007/BF00194278

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

567-74

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

183

pub_type

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