Enzyme activities in mitochondria isolated from ripening tomato fruit.

Abstract:

:Mitochondria were isolated from tomato (Lycopersicon esculentum L.) fruit at the mature green, orange-green and red stages and from fruit artificially suspended in their ripening stage. The specific activities of citrate synthase (EC 4.1.3.7), malate dehydrogenase (EC 1.1.1.37), NAD-linked isocitrate dehydrogenase (EC 1.1.1.41) and NAD-linked malic enzyme (EC 1.1.1.38) were determined. The specific activities of all these enzymes fell during ipening, although the mitochondria were fully functional as demonstrated by the uptake of oxygen. The fall in activity of mitochondrial malate dehydrogenase was accompanied by a similar fall in the activity of the cytosolic isoenzyme. Percoll-purified mitochondria isolated from mature green fruit remained intact for more than one week and at least one enzyme, citrate synthase, did not exhibit the fall in specific activity found in normal ripening fruit.

journal_name

Planta

journal_title

Planta

authors

Jeffery D,Goodenough PW,Weitzman PD

doi

10.1007/BF00392366

subject

Has Abstract

pub_date

1986-09-01 00:00:00

pages

390-4

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

168

pub_type

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