Abstract:
:The mechanism for cadmium (Cd2+) uptake into the cytosol of protoplasts from 5- to 7-day-old wheat seedlings (Triticum aestivum L. cv. Kadett) was investigated by a new method, using fluorescence microscopy and the heavy metal-specific fluorescent dye, 5-nitrobenzothiazole coumarin, BTC-5N. Cadmium fluorescence gradually increased in the cytosol of shoot and root protoplasts upon repeated additions of CdCl2 to the external medium, reflecting an uptake of Cd2+. The uptake was inhibited by calcium and potassium chloride, as well as by Verapamil and tetraethylammonium (TEA), inhibitors of calcium and potassium channels, respectively. Calcium competitively inhibited the cadmium uptake. The metabolic inhibitors vanadate and dinitrophenol partly inhibited the uptake, suggesting it was dependent on membrane potential. The results indicate that cadmium is taken up by channels permeable to both calcium and potassium. The total uptake of cadmium into the protoplasts was also detected by unidirectional flux analyses using 109Cd2+, and showed approximately the same maximal concentration of Cd2+ as the fluorescence measurements. By combining the two methods it is possible to detect both uptake into the cytosol and into the vacuole.
journal_name
Plantajournal_title
Plantaauthors
Lindberg S,Landberg T,Greger Mdoi
10.1007/s00425-004-1256-zsubject
Has Abstractpub_date
2004-07-01 00:00:00pages
526-32issue
3eissn
0032-0935issn
1432-2048journal_volume
219pub_type
杂志文章相关文献
PLANTA文献大全abstract::Narbonin is a 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons. Its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. Therefore, it was expected to be present in protein bodies o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00050163
更新日期:1997-09-01 00:00:00
abstract::Malic and citric acids accumulate in cherry tomato (Lycopersicon esculentum Mill.) fruit during the period of rapid growth, from the end of cell division to the onset of ripening. The involvement of phospho enolpyruvate carboxylase (PEPCase, EC 4.1.1.31) in organic acid accumulation and tomato fruit development was in...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-001-0687-z
更新日期:2002-03-01 00:00:00
abstract:MAIN CONCLUSION:Rhizobial symbiosis improved the water status of bean plants under salinity-stress conditions, in part by increasing their osmotic root water flow. One of the main problems for agriculture worldwide is the increasing salinization of farming lands. The use of soil beneficial microorganisms stands up as a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-03076-0
更新日期:2019-04-01 00:00:00
abstract:MAIN CONCLUSION:Putative XyG xylosyltransferases from Tropaeolum majus (nasturtium) and Solanum lycopersicum (tomato) homologous to characterized Arabidopsis genes were identified and shown to functionally complement Arabidopsis mutants lacking xyloglucan demonstrating they represent xyloglucan xylosyltransferases. Xyl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2243-2
更新日期:2015-05-01 00:00:00
abstract::The major fatty acid component of castor (Ricinus communis L.) oil is ricinoleic acid (12-hydroxy-cis-9-octadecenoic acid), and unsaturated hydroxy acid accounts for >85% of the total fatty acids in triacylglycerol (TAG). TAG had a higher ricinoleate content at position 2 than at positions 1 and 3. Although lysophosph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0083-1
更新日期:2006-01-01 00:00:00
abstract::Cycloheximide (CH) was applied selectively either to the shoot apex or by infiltration to the leaves of the long-day plant Hyoscyamus niger in order to investigate whether this inhibitor has an effect on the synthesis of a floral stimulus in the leaves. Treatment of the shoot apex with CH caused inhibition of the phot...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386028
更新日期:1973-03-01 00:00:00
abstract::Described here are the first quantitative measurements of absolute amounts of mRNAs transcribed from individual members of a phytochrome gene (PHY) family. The abundances of PHY mRNAs were determined for dry seed and for selected organs of green-house-grown tomato (Solanum lycopersicum L.) seedlings and mature plants....
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050080
更新日期:1997-01-01 00:00:00
abstract::When radioactive gibberellin A5 ((3)H-GA5) was applied to the apices and surrounding young leaves of the long-day plant Silene armeria, it was partially converted to at least two other acidic substances. One of them was similar to GA3 in chromatographic, but not in biological properties. The other metabolite was more ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385349
更新日期:1971-06-01 00:00:00
abstract::Ascorbic acid (AsA) plays an essential role in plants by protecting cells against oxidative damage. GDP-L-galactose phosphorylase (GGP) is the first committed gene for AsA synthesis. Our research examined AsA levels, regulation of GGP gene expression, and how these are related to abiotic stresses in two species of Act...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1915-z
更新日期:2013-09-01 00:00:00
abstract::Active Cl(-) uptake by Chlorella fusca was examined by using (36)Cl as a label. Under light/air conditions chloride influx from a 2.4·10(-5) M solution was 4.0±0.04 nmol m(-2)s(-1). After 70±10 min a stationary 380±40 fold accumulation was reached. In dark/air and dark/argon influx and accumulation were reduced to 25±...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393437
更新日期:1982-11-01 00:00:00
abstract:MAIN CONCLUSION:We show that changing the expression of a putative feruloyl transferase gene belonging to the BAHD acyl-transferase family alters the levels of cell wall esterified ferulates and diferulates in Brachypodium distachyon cell walls. While the potential of grass cell walls for biofuel production has been re...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2430-1
更新日期:2016-03-01 00:00:00
abstract::The rate of total phytochrome decay in the dicotyledons Amaranthus caudatus, Mirabilis jalapa and Pisum sativum under continuous illumination with red, incandescent, and blue light depends on the PFR/Ptotal maintained by each source. Amaranthus is an exception to this in that there is a deviation from firstorder decay...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386614
更新日期:1972-12-01 00:00:00
abstract::Preparations of rapidly labelled RNA are usually heavily contaminated by rapidly labelled low molecular weight components. This contaminating fraction mainly contains nucleoside triphosphates, as previously reported, but also nucleoside tetra, penta and hexaphosphates. These highly phosphorylated nucleosides are, acco...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385426
更新日期:1976-01-01 00:00:00
abstract::The carbon-dioxide response of photosynthesis of leaves of Quercus suber, a sclerophyllous species of the European Mediterranean region, was studied as a function of time of day at the end of the summer dry season in the natural habitat. To examine the response experimentally, a "standard" time course for temperature ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397440
更新日期:1984-09-01 00:00:00
abstract::The binding of concanavalin A (con A) to leek (Allium porrum L.) stem protoplasts has been investigated using sequential treatment with con A and haemocyanin and using con A covalently linked to ferritin. Prefixed protoplasts were evenly labelled. Unfixed protoplasts showed a clustered distribution of label. Low tempe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388761
更新日期:1979-01-01 00:00:00
abstract:MAIN CONCLUSION:Variable cotton fiber diameter is set early in anisotropic elongation by cell-type-specific processes involving the temporal and spatial regulation of microtubules in the apical region. Cotton fibers are single cells that originate from the seed epidermis of Gossypium species. Then, they undergo extreme...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03557-1
更新日期:2021-01-23 00:00:00
abstract::The circadian movement of the lamina of primary leaves of Phaseolus coccineus L. is mediated by antagonistic changes in the length of the extensor and flexor cells of the laminar pulvinus. The cortex of the pulvinus is a concentric structure composed of hexagonal disc-like cells, arranged in longitudinal rows around t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395147
更新日期:1985-03-01 00:00:00
abstract::A study has been made of plastids in the root meristem of Colchicum autumnale. These organelles contain simultaneously deposits of starch, lipid, and a crystalline material, probably protein. Two types of internal membrane are present within the plastid. One appears to be concerned with the transport of material acros...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00389294
更新日期:1969-03-01 00:00:00
abstract::Lipid extracts from Sorghum sudanese Stapf (cv Piper) that had been subjected to different degrees of water stress contained increasing amounts of a sesquiterpenoid, other than abscisic acid, as the amount of stress increased and subsequent bioassay showed that this substance was capable of inducing stomatal closure. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390293
更新日期:1974-01-01 00:00:00
abstract::Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00380870
更新日期:1980-07-01 00:00:00
abstract::Thermogenesis during the blooming of inflorescence is found in several but not all aroids. To understand what is critical for thermogenesis, we investigated the difference between thermogenic and non-thermogenic skunk cabbages (Symplocarpus renifolius and Lysichiton camtschatcensis), which are closely related in morph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-1034-z
更新日期:2009-12-01 00:00:00
abstract::Isoelectric focusing of the membrane polypeptides derived from castor bean (Ricinus communis L.) endosperm glyoxysomes and endoplasmic reticulum reveals a close similarity in the composition of these membranes, consistent with a developmental relationship between the organelles. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390852
更新日期:1976-01-01 00:00:00
abstract::The qualitative and quantitative composition of the carotenoids of young cells of Euglena gracilis strain 1224-5/9 corresponded to that of the "Z"-strain.The frequently observed yellow-reddish discolouration of ageing cells was found to be caused by a heavy breakdown of the chlorophylls and not by an increased synthes...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387621
更新日期:1971-06-01 00:00:00
abstract::Cutin and suberin polymers from various anatomical regions of grapefruit were analyzed chemically and ultrastructurally. The leaf, fruit peel and juice-sac showed an amorphous cuticular layer. The cutin in the leaf was composed of 10,16-dihydroxy C16 acid and its positional isomers as the major monomers whereas 16-hyd...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385755
更新日期:1980-10-01 00:00:00
abstract::When barley aleurone layers are treated with gibberellic acid (GA3) in the presence of increasing concentrations (0.2-0.8 M) of mannitol, the rate of (32)Pi incorporation into phospholipids becomes progressively inhibited. Mannitol does not affect this process in aleurone layers not treated with GA3, nor does it appre...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387082
更新日期:1972-09-01 00:00:00
abstract::cis-Zeatin riboside was identified in transfer-RNA hydrolysates from both normal and crown-gall tissue of Vinca rosea L. The trans-isomer was associated exclusively with the crowngall transfer-RNA. The importance of these observations is discussed in relation to biosynthesis of free cytokinins. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392990
更新日期:1983-10-01 00:00:00
abstract::Plants of Monstera deliciosa Liebm. pruned to exemplars with one leaf and one aerial root were labeled with 7.4 MBq (14)CO2 over the leaf blade. Microautoradiographs of soluble and insoluble radioactivity were prepared from three different regions of the aerial root. In addition, histochemical localization of ATPase w...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00396886
更新日期:1983-05-01 00:00:00
abstract::Whether cell division is a driving force in plant morphogenesis has long been debated. In this review, the evidence for the existence of cell division-dependent and cell division-independent mechanisms of plant morphogenesis is discussed. The potential mechanisms themselves are then analysed, as is our understanding o...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-002-0864-8
更新日期:2002-11-01 00:00:00
abstract::The ontogenetic change taking place in the facultative photoperiodic response of A. retroflexus to inductive short-day (SD) conditions was studied by exposing plants to continuous induction after different initial exposures to long-days (LD), and comparing the kinetics of their developmental responses (cumulative numb...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387918
更新日期:1977-01-01 00:00:00
abstract::A soluble extract from pea (Pisum sativum L.) roots, when incubated with ATP and inositol 1,4,5-trisphosphate, produced an inositol tetrakisphosphate. The chromatographic properties of this inositol tetrakisphosphate, and of the products formed by its chemical degradation, identify it as inositol 1,4,5,6-tetrakisphosp...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00199975
更新日期:1992-07-01 00:00:00