Abstract:
:The binding of concanavalin A (con A) to leek (Allium porrum L.) stem protoplasts has been investigated using sequential treatment with con A and haemocyanin and using con A covalently linked to ferritin. Prefixed protoplasts were evenly labelled. Unfixed protoplasts showed a clustered distribution of label. Low temperature and lanthanum reduced the clustering. Bound con A was lost from unfixed protoplasts incubated for 5 h after treatment, but con A/haemocyanin was not bound to nascent wall materials. Prefixed protoplasts treated with wall-removing enzymes before labelling showed only a small reduction of con A binding. These results indicate that con A is bound to plasma membrane components, but that binding is reduced by competition of nascent wall precursors.
journal_name
Plantajournal_title
Plantaauthors
Williamson FAdoi
10.1007/BF00388761subject
Has Abstractpub_date
1979-01-01 00:00:00pages
209-15issue
3eissn
0032-0935issn
1432-2048journal_volume
144pub_type
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