Molecular cloning and characterisation of cDNAs complementary to mRNAs from wounded potato (Solanum tuberosum) tuber tissue.

Abstract:

:Five cDNA clones complementary to mRNAs representing different abundances and responses to wounding have been isolated from a library of Sau 3A fragments in the bacteriophage M13 mp8. These were characterised by hybrid-release translation and hybridisation to RNA blots. The levels of RNA complementary to two of the clones show a marked increase during the 24h after wounding, one shows a small increase and two show no appreciable changes except that caused by a general increase in the total amount of polyadenylated RNA per microgram of total RNA which increases 2.5-fold during the same period. The would-induced RNAs are not induced in diluted suspension-culture cells, but RNA complementary to each clone is present in varying levels in stems, leaves and roots of intact potato plants.

journal_name

Planta

journal_title

Planta

authors

Shirras AD,Northcote DH

doi

10.1007/BF00396748

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

353-60

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

162

pub_type

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