Abstract:
:Oilseed rape (Brassica napus L.) needs very high nitrogen fertilizer inputs. Significant amounts of this nitrogen are lost during early leaf shedding and are a source of environmental and economic concern. The objective of this study was to investigate whether the remobilization of leaf amino acids could be limiting for nitrogen use efficiency. Therefore, amino acid concentrations were analyzed in subcellular compartments of leaf mesophyll cells of plants grown under low (0.5 mM NO3-) and high (4 mM NO3-) nitrogen supply. With high nitrogen supply, young leaves showed an elevated amino acid content, mainly in vacuoles. In old leaves, however, subcellular concentrations were similar under high and low nitrogen conditions, showing that the excess nitrogen had been exported during leaf development. The phloem sap contained up to 650 mM amino acids, more than four times as much than the cytosol of mesophyll cells, indicating a very efficient phloem-loading process. Three amino acid permeases, BnAAP1, BnAAP2, and BnAAP6, were identified and characterized. BnAAP1 and BnAAP6 mediated uptake of neutral and acidic amino acids into Xenopus laevis oocytes at the actual apoplastic substrate concentrations. All three transporters were expressed in leaves and the expression was still detectable during leaf senescence, with BnAAP1 and BnAAP2 mRNA levels increasing from mature to old leaves. We conclude that phloem loading of amino acids is not limiting for nitrogen remobilization from senescing leaves in oilseed rape.
journal_name
Plantajournal_title
Plantaauthors
Tilsner J,Kassner N,Struck C,Lohaus Gdoi
10.1007/s00425-004-1446-8subject
Has Abstractpub_date
2005-06-01 00:00:00pages
328-38issue
3eissn
0032-0935issn
1432-2048journal_volume
221pub_type
杂志文章相关文献
PLANTA文献大全abstract::Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l myoinositol, 0.1 mg/l thiami...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392306
更新日期:1987-08-01 00:00:00
abstract::A monoclonal antibody produced to abscisic acid (ABA) has been characterised and the development of a radioimmunoassay (RIA) for ABA using the antibody is described. The antibody had a high selectivity for the free acid of (S)-cis, trans-ABA. Using the antibody, ABA could be assayed reliably in the RIA over a range fr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00401020
更新日期:1988-03-01 00:00:00
abstract::The plant hormone, jasmonic acid (JA), is known to have a critical role in both resistance and susceptibility against bacterial and fungal pathogen attack. However, little is known about the involvement of JA in the interactions between plants and toxigenic necrotrophic fungal pathogens. Using the tomato pathotype of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0890-x
更新日期:2009-03-01 00:00:00
abstract::Diseases of agricultural crops are caused by pathogens from several higher-order phylogenetic lineages including fungi, straminipila, eubacteria, and metazoa. These pathogens are commonly managed with pesticides due to the lack of broad-spectrum host resistance. Gastrodia anti-fungal protein (GAFP; gastrodianin) may p...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0322-0
更新日期:2006-11-01 00:00:00
abstract::Root growth was analyzed in Allium cepa L. grown under steady-state conditions at 10, 15 and 25°C by measuring cell sizes along the meristem and mature zones at these three temperatures and in the elongation zone at 15°C. The absolute rate of growth, v(x), was determined as a function of the mean cell number of cells ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00402962
更新日期:1986-06-01 00:00:00
abstract::The rate of total phytochrome decay in the dicotyledons Amaranthus caudatus, Mirabilis jalapa and Pisum sativum under continuous illumination with red, incandescent, and blue light depends on the PFR/Ptotal maintained by each source. Amaranthus is an exception to this in that there is a deviation from firstorder decay...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386614
更新日期:1972-12-01 00:00:00
abstract::The effects of methyl jasmonate and jasmonic acid on uptake of abscisic acid (ABA) by suspension-cultured runner-bean cells and subapical runner-bean root segments have been investigated. Increasing concentrations of methyl jasmonate inhibit ABA uptake by the cultured cells with a K i of 22±3 μM. This is not due to cy...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397356
更新日期:1985-10-01 00:00:00
abstract:MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2431-0
更新日期:2016-03-01 00:00:00
abstract::The induction of monospore-development in the Conchocelis stage of Porphyra tenera KJELLM. was investigated. 1. Experiments with light-breaks during the dark-periods indicate the decisive role of a photoperiodic control. 2. The critical daylength is approximately 10 hr. Daily light periods longer than 10 hrs inhibit t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387423
更新日期:1967-03-01 00:00:00
abstract::Vomifoliol, a compound related to abscisic acid (ABA) but lacking the 2,4-pentadiene sidechain and so far found in four different plant families, caused as much stomatal closure in epidermal strips of Eichhornia crassipes (Mart.) Solms as ABA at identical concentrations. It appears worthwhile to investigate whether vo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385281
更新日期:1975-01-01 00:00:00
abstract::In shoot tip-derived tissue cultures of Scots pine (Pinus sylvestris L.), browning and subsequent degeneration of the culture is accompanied by lipid peroxidation and lignification of cells, which are characteristic features of a plant defense reaction. Since chitinases are enzymes acting primarily in plant defense, t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-001-0709-x
更新日期:2002-04-01 00:00:00
abstract:MAIN CONCLUSION:We demonstrate that rbcL underwent strong positive selection during the C 3 -C 4 photosynthetic transitions in PACMAD grasses, in particular the 3' end of the gene. In contrast, selective pressures on other plastid genes vary widely and environmental drivers remain to be identified. Plastid genomes have...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2781-x
更新日期:2018-01-01 00:00:00
abstract:MAIN CONCLUSION:Global warming will increase the incidence of metabolism-based reduced herbicide efficacy on weeds and, therefore, the risk for evolution of non-target site herbicide resistance. Climate changes affect food security both directly and indirectly. Weeds are the major biotic factor limiting crop production...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2577-4
更新日期:2016-12-01 00:00:00
abstract::α-Galactosidase II(2) (MW ∼ 43 390) from resting Vicia faba L. seeds had been shown to possess D-glucose/D-mannose-specific lectin activity. Inhibition studies with monosaccharides and an examination of the effects of heat and pH on the catalytic and lectin activities of the enzyme indicate that the enzyme substrate a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00446377
更新日期:1986-01-01 00:00:00
abstract::Taking into account the literature on the so-called sieve-tube slime ("mictoplasm", "slime strands") and regarding its fine structure more in detail the term plasmatic filament ("Plasmafilament") is proposed and will be used in this paper to characterize the individual exceedingly fine subunit of the plasmatic network...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385169
更新日期:1967-03-01 00:00:00
abstract:MAIN CONCLUSION:This study provides new insights that alternative splicing participates with transcriptional control in defense responses to Colletotrichum sublineola in sorghum In eukaryotic organisms, alternative splicing (AS) is an important post-transcriptional mechanism to generate multiple transcript isoforms fro...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-019-03309-w
更新日期:2019-11-27 00:00:00
abstract::Phosphoenolpyruvate (PEP) carboxylase has been isolated from etiolated and light-grown sugar cane, and a comparison made of the physical and kinetic characteristics of the enzymes. Differences were found in the DEAE-cellulose elution profiles, in saturation kinetics with PEP as variable substrate and with Mg(2+) as va...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388679
更新日期:1974-03-01 00:00:00
abstract:MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03435-w
更新日期:2020-08-01 00:00:00
abstract::Eukaryotic genomes harbor mobile genetic elements known as long terminal repeat (LTR) retrotransposons. LTR retrotransposons are closely related to the infectious and endogenous retroviruses. The viral envelope (env) gene of the retroviruses, which is responsible for their infective properties, distinguishes them from...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0903-5
更新日期:2002-12-01 00:00:00
abstract:MAIN CONCLUSION:The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated fro...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2373-6
更新日期:2015-12-01 00:00:00
abstract::The glucosinolate composition and content in various tissues of Arabidopsis thaliana (L.) Heynh. ecotype Columbia during development from seeds to bolting plants were determined in detail by high-performance liquid chromatography. Comparison of the glucosinolate profiles of leaves, roots and stems from mature plants w...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100659
更新日期:2002-02-01 00:00:00
abstract::A full-length cDNA coding for a symbiosis-regulated transcript, EgHypar, was isolated by differential screening from a Eucalyptus globulus bicostata--Pisolithus tinctorius ectomycorrhiza. The sequence of this clone revealed a protein with an estimated molecular mass of 25.5 kDa that exhibited a high degree of homology...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050486
更新日期:1998-12-01 00:00:00
abstract::A 78-amino acid insertion (L78) is found in the low-affinity type (L-form) of starch phosphorylase (L-SP, EC 2.4.1.1). This insertion blocks the starch-binding site on the L-SP molecule, and it decreases the binding affinity of L-SP toward starch. The computational analysis of the amino acid sequence on L78 predicts s...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0103-1
更新日期:2006-02-01 00:00:00
abstract::1-Aminocyclopropane-1-carboxylic acid synthase (ACS) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. ACS isozymes are regulated both transcriptionally and post-translationally. However, in banana, an important climacteric fruit, little is known about post-translational regulation of ACS. Her...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1627-9
更新日期:2012-08-01 00:00:00
abstract::Discs punched out of different zones of the found of Laminaria hyperborea (Gunn.) Fossl. were exposed to H(14)CO3 (-) in the light and in the dark for various lengths of time. Photosynthetic rates in young and old parts were in the same range, 12-39 μmol CO2 dm(-2) h(-1) at 4°C, whereas dark fixation was remarkably hi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388702
更新日期:1975-01-01 00:00:00
abstract::Treatment of cress (Lepidium sativum L.) roots with phytohormones (4.3 x 10(-5) M gibberellic acid plus 4.3 x 10(-5) M kinetin, 30 h; T.H. Iversen, 1969, Physiol. Plant. 22, 1251-1262) caused not only complete destarching of amyloplasts but also destruction of the polar arrangement of cell organelles in statocytes. Th...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195888
更新日期:1990-06-01 00:00:00
abstract::Changes in membrane topography in the flagellate Poterioochromonas malhamensis, as a result of colchicine and osmotic-stress treatments, have been studied using freeze-fracturing and thin sectioning. Ridges, but not rows of intramembrane particles, in the PF-face which denote the position of underlying cortical microt...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385446
更新日期:1980-02-01 00:00:00
abstract::The synthesis of a major phloem protein, PP2, was investigated by measurement of the mRNA at various stages of phloem development in Cucurbita. Quantitative assays with immuno-electrophoresis showed that the amounts of PP2 in hypocotyls of Cucurbita seedlings increased with the age of seedlings. An increase in mRNA fo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395032
更新日期:1987-03-01 00:00:00
abstract:MAIN CONCLUSION:Three novel QTLs for grain shape were genetically fine mapped, with two of which to a 250-kb target interval on rice chromosome 2 that contains fourteen candidate genes. Grain shape (grain length, width, and thickness) determines crop yield and grain quality. However, the trait is regulated by numerous ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03420-3
更新日期:2020-07-15 00:00:00
abstract::Polyamine levels and the activities of two polyamine biosynthetic enzymes, arginine decarboxylase (EC 4.1.1.19) and S-adenosylmethionine decarboxylase (EC 4.1.1.50), were determined during somatic embryogenesis of carrot (Daucus carota L.) cell cultures. Embryogenic cultures showed severalfold increases in polyamine l...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00399919
更新日期:1984-12-01 00:00:00