Abstract：
MAIN CONCLUSION:This study provides new insights that alternative splicing participates with transcriptional control in defense responses to Colletotrichum sublineola in sorghum In eukaryotic organisms, alternative splicing (AS) is an important post-transcriptional mechanism to generate multiple transcript isoforms from a single gene. Protein variants translated from splicing isoforms may have altered molecular characteristics in signal transduction and metabolic activities. However, which transcript isoforms will be translated into proteins and the biological functions of the resulting proteoforms are yet to be identified. Sorghum is one of the five major cereal crops, but its production is severely affected by fungal diseases. For example, sorghum anthracnose caused by Colletotrichum sublineola greatly reduces grain yield and biomass production. In this study, next-generation sequencing technology was used to analyze C. sublineola-inoculated sorghum seedlings compared with mock-inoculated control. It was identified that AS regulation may be as important as traditional transcriptional control during defense responses to fungal infection. Moreover, several genes involved in flavonoid and phenylpropanoid biosynthetic pathways were found to undergo multiple AS modifications. Further analysis demonstrated that non-conventional targets of both 5'- and 3'-splice sites were alternatively used in response to C. sublineola infection. Splicing factors were also affected at both transcriptional and post-transcriptional levels. As the first transcriptome report on C. sublineola infected sorghum, our work also suggested that AS plays crucial functions in defense responses to fungal invasion.
journal_name
Plantajournal_title
Plantaauthors
Wang L,Chen M,Zhu F,Fan T,Zhang J,Lo Cdoi
10.1007/s00425-019-03309-wsubject
Has Abstractpub_date
2019-11-27 00:00:00pages
14issue
1eissn
0032-0935issn
1432-2048pii
10.1007/s00425-019-03309-wjournal_volume
251pub_type
杂志文章相关文献
PLANTA文献大全abstract：:The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to alpha-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. P...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-1129-x
更新日期：2004-03-01 00:00:00
abstract：:The sequence of events leading up to the establishment of symbiotic nitrogen-fixation were studied in two tropical legumes, Centrosema pubescens Benth, and Vigna unguiculata L. Walp. Parameters measured included fresh and dry weights, chlorophyll and leghaemoglobin contents, as well as the activities of NADH-nitrate r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387146
更新日期：1978-01-01 00:00:00
abstract：:The adaxial leaf cuticle of Malus pumila was examined by electron microscopy to determine possible avenues for transcuticular movement of foliarly applied chemicals. Cutin-embedded polysaccharide microfibrils originated at the outer epidermal cell wall and occasionally extended to the cuticle surface. Lamellae, ca. 4 ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388736
更新日期：1979-12-01 00:00:00
abstract：:This study investigates mechanisms of multiple resistance to glyphosate, acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS)-inhibiting herbicides in two Lolium rigidum populations from Australia. When treated with glyphosate, susceptible (S) plants accumulated 4- to 6-fold more shikimic acid than r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-0981-8
更新日期：2009-09-01 00:00:00
abstract：:In crude extracts of primary leaves from dark grown seedlings of Phaseolus vulgaris L., relatively high hydrolytic activity of chlorophyllase (chlorophyll-chlorophyllido-hydrolase, EC 3.1.1.14) was observed. When plants were exposed to continuous illumination, the enzyme activity in the extracts was doubled within 3 d...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390814
更新日期：1978-01-01 00:00:00
abstract：:Agrobacterium tumefaciens-induced tumors of dicotyledonous plants consist of well-defined vascular bundle-like structures originating from transformed cells. The current view that 25% of the tumor cells are transformed has been re-investigated by using beta-glucuronidase (gus)-gene-containing wild-type bacteria (A281 ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050742
更新日期：1999-10-01 00:00:00
abstract：:Enhancement of carbon fixation was demonstrated in the bluegreen alga, Anabaena cylindrica, grown in either aerobic or microaerobic conditions. Under identical conditions no enhancement of acetylene reduction was observed. Light absorbed by photosystem I supported relatively more acetylene reduction than carbon fixati...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385450
更新日期：1973-03-01 00:00:00
abstract：:Intact chloroplasts capable of high rates of CO2 assimilation completely oxidized 3-phosphoglycerate and dihydroxyacetone phosphate to glycolate when CO2 concentrations were low. Bicarbonate was converted first into products of the Calvin cycle and then into glycolate. Under high oxygen and at high pH values CO2 fixat...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388894
更新日期：1976-01-01 00:00:00
abstract：:Illumination of a liquid culture of Synechococcus 6301 at high photon flux density (PFD) elicits a time-dependent first-order exponential decline in relative quantum yield of photosynthetic O2 evolution to some steady-state value. Full photosynthetic activity is restored, also as a time-dependent first-order process, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00195324
更新日期：1992-02-01 00:00:00
abstract：:Quinolizidine alkaloids formed in the leaves of Lupinus albus L. are translocated via the phloem to the other plant organs, especially the maturing fruits. Compared with amino-acid transport in the phloem, the alkaloids contribute about 8% to the overall nitrogen being exported from the leaf. Since it is likely that t...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00407083
更新日期：1984-11-01 00:00:00
abstract：:A study was made of the day-night changes under controlled environmental conditions in the bulk-leaf water relations of Kalanchoë daigremontiana, a plant showing Crassulacean acid metabolism. In addition to nocturnal stomatal opening and net CO2 uptake, the leaves of well-watered plants showed high rates of gas exchan...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00393518
更新日期：1985-02-01 00:00:00
abstract：:Polyribosomes which have template activity in the wheat germ system have been isolated from developing pea seeds. Some of the translation products have identical mobilities to the vicilin and legumin subunits by SDS-PAGE. Certain products were specifically immunoprecipitated with antisera prepared against purified vic...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00380122
更新日期：1979-01-01 00:00:00
abstract：:The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by fa...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00397734
更新日期：1983-08-01 00:00:00
abstract：:The control of rhythmic emission of floral volatiles emitted from Rosa damascena semperflorens cv. 'Quatre Saisons' throughout floral development under various light regimes was studied. 2-Phenylethanol was the major volatile emitted in addition to monoterpenols, oxidised monoterpenols, monoterpenes and aromatic compo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1250-5
更新日期：2004-07-01 00:00:00
abstract：MAIN CONCLUSION:Phytosterol homeostasis may be maintained in leaves through diversion of intermediates into glycoalkaloid biosynthesis, whereas in tuber flesh, excess intermediates are catalyzed by tuber-specific StLAS - like , resulting in low tuber glycoalkaloids. Lanosterol synthase (LAS) and cycloartenol synthase (...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-017-2763-z
更新日期：2017-12-01 00:00:00
abstract：:The nuclei of active, sieve cell-associated Strasburger cells in the secondary phloem of Pinus nigra var. austriaca (Hoess) Badoux have been studied for their structure and DNA and RNA content. No difference in size compared to those of ordinary ray cells was found. The nuclear surface is often increased by an ameboid...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00394427
更新日期：1977-01-01 00:00:00
abstract：:A particulate preparation from developing cotyledons of Phaseolus vulgaris L. was incubated with uridine-5'-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc; [6-(3)H]glucosamine), and by polyacrylamide gel electrophoretic analysis it was shown that the labeled (N-acetyl)glucosamine (GlcNAc) was incorporated into the princ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00380867
更新日期：1979-09-01 00:00:00
abstract：:Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino acids through the expression of heterologous proteins have met with limite...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-011-1487-8
更新日期：2012-01-01 00:00:00
abstract：:The role of phloem anastomoses in translocation was studied experimentally in intact and wounded internodes ofDahlia pinnata Cav. Translocation was visualized with fluorescein, a fluorescent dye capable of movement in the phloem. Translocation was analyzed in large areas of living phloem tissue which were peeled from ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF02341035
更新日期：1990-11-01 00:00:00
abstract：MAIN CONCLUSION:Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell d...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-016-2619-y
更新日期：2017-03-01 00:00:00
abstract：:Sphagnum magellanicum shows a remarkable variation in colour after the repeated application of cold shocks; mainly red wall pigments are synthesized. An attempt was made to find out in which way the gas exchange of Sphagnum magellanicum under defined conditions is altered after the application of cold shocks.The light...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388819
更新日期：1968-03-01 00:00:00
abstract：:The vacuolar pH of individual cells of the epidermis of intact leaves of Commelina communis L. has been determined using pH sensitive microelectrodes. The results show that the pH of the guard cells increases by approximately 0.5 units during stomatal opening. The changes in vacuolar pH mirror the changes in vacuolar ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388660
更新日期：1975-01-01 00:00:00
abstract：:The aim of this work was to examine the metabolism of exogenous gluconate by a 4-day-old cell suspension culture of Arabidopsis thaliana (L.) Heynh. Release of (14)CO(2) from [1-(14)C]gluconate was dependent on the concentration in the medium and could be resolved into a substrate-saturable component (apparent K(m) of...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0842-1
更新日期：2002-12-01 00:00:00
abstract：MAIN CONCLUSION:Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-2879-9
更新日期：2018-06-01 00:00:00
abstract：:Endogenous gibberellins were obtained in agar from the lower cut surface of upright sunflower shoot-tips. Exposure to unilateral light of the tips standing on agar, with the lower cut ends bisected by a vertical glass barrier at right angles to incident light, resulted in approximately 8 times the quantity of gibberel...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384773
更新日期：1972-12-01 00:00:00
abstract：:The development of maximal rates of lipid and protein hydrolysis in cucumber cotyledons depends upon the removal of the testa and the presence of the embryonic axis. The testa appears to exert at least part of its inhibitory influence by suppressing the development of enzyme activity associated with lipolysis and prot...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388835
更新日期：1979-10-01 00:00:00
abstract：:Galls induced on Gypsophila paniculata by Pantoea agglomerans pv. gypsophilae (Pag) and Agrobacterium tumefaciens (At), bacteria with different mechanisms of pathogenicity, were compared morphologically and anatomically. The pathogenicity of Pag is dependent on the presence of an indigenous plasmid that harbors hrp ge...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0229-9
更新日期：2006-07-01 00:00:00
abstract：:Isolated embryos are more suitable for in vivo study of protein synthesis than non-isolated embryos because, after isolation, the uptake of labeled amino acids is about 1000 times higher. However, isolation also stimulates protein synthesis: Up to 4 h after isolation, the capacity to incorporate labeled amino acids in...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385536
更新日期：1981-12-01 00:00:00
abstract：:Internode segments excised from vegetative stems of Plumbago indica, an absolute short-day plant, can be induced to form in vitro a callus, or roots, or vegetative shoots, or inflorescences and flowers. Callus formation was polarized when indolyl-3-acetic acid, L-tryptophane, naphthylacetamide, or 2,3-dichlorophenylac...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390146
更新日期：1967-12-01 00:00:00
abstract：:The bud-inducing effect of the cytokinin N(6)-(Δ(2)-isopentenyl)-adenine (i(6)-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i(6)-Ade, bud-formation was accelerated in both types ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392232
更新日期：1985-08-01 00:00:00
