Increase of protein synthesis after isolation in dormant and afterripened Agrostemma githago L. embryos.

Abstract:

:Isolated embryos are more suitable for in vivo study of protein synthesis than non-isolated embryos because, after isolation, the uptake of labeled amino acids is about 1000 times higher. However, isolation also stimulates protein synthesis: Up to 4 h after isolation, the capacity to incorporate labeled amino acids increased 7 times. Therefore, data on incorporation obtained with isolated embryos cannot be extended to non-isolated embryos. The increase of protein synthesis was not due to synthesis of specific proteins, but was a general increase. Furthermore, ripening, dormant, and afterripened embryos showed the same degree of increase. Isolation therefore stimulates protein synthesis nonspecifically. When embryos were kept under anaerobic conditions after isolation, protein synthesis did not increase. Therefore, higher oxygen consumption after removal of the seedcoat is probably the cause of the higher incorporation capacity. Furthermore, the activation of protein synthesis lagged several hours behind the increase of oxygen consumption.

journal_name

Planta

journal_title

Planta

authors

de Klerk GJ

doi

10.1007/BF00385536

subject

Has Abstract

pub_date

1981-12-01 00:00:00

pages

524-9

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

153

pub_type

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