Cell free synthesis of some storage protein subunits by polyribosomes and RNA isolated from developing seeds of pea (Pisum sativum L.).

Abstract:

:Polyribosomes which have template activity in the wheat germ system have been isolated from developing pea seeds. Some of the translation products have identical mobilities to the vicilin and legumin subunits by SDS-PAGE. Certain products were specifically immunoprecipitated with antisera prepared against purified vicilin and legumin fractions. Various RNA fractions including poly A-rich RNA have also been isolated from polyribosomes and shown to direct the synthesis of polyripeptides whose properties are similar to the storage protein subunits. The results are discussed in relationship to other investigations with seed storage protein biosynthesis in vitro.

journal_name

Planta

journal_title

Planta

authors

Marta Evans I,Croy RR,Hutchinson P,Boulter D,Payne PI,Gordon ME

doi

10.1007/BF00380122

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

455-62

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

144

pub_type

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