Abstract:
:Monoclonal antibodies have been raised against haustorial complexes isolated from pea (Pisum sativum L.) leaves infected by the biotrophic powdery mildew fungus Erysiphe pisi D.C. Immuno-localisation studies, using isolated haustorial complexes and infected pea leaf material, have shown that one of the antibodies, designated UB7, binds to fungal wall and plasma membranes present in both haustoria and mycelia. However, a second antibody, UB8, binds specifically to the haustorial plasma membrane, and does not label fungal plasma membranes in mycelia. Western blotting and antigen-modification techniques have shown that UB8 recognises a protein epitope of a 62-kDa antigen. A reduction in molecular weight of this component after endo-F treatment indicates that the antigen is an N-linked glycoprotein. UB7 also recognises a 62-kDa glycoprotein, which is susceptible to endo-F treatment, and the antibody binds to a carbohydrate epitope. Differences in molecular weights of the products after endo-F treatment of antigens show that the 62-kDa glycoproteins recognised by the antibodies are distinct molecules, in accordance with the localisation results. Overall, the results provide evidence for molecular differentiation associated with the development of haustoria in a biotrophic infection.
journal_name
Plantajournal_title
Plantaauthors
Mackie AJ,Roberts AM,Callow JA,Green JRdoi
10.1007/BF00197739subject
Has Abstractpub_date
1991-02-01 00:00:00pages
399-408issue
3eissn
0032-0935issn
1432-2048journal_volume
183pub_type
杂志文章相关文献
PLANTA文献大全abstract::Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Ra...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0699-z
更新日期:2008-05-01 00:00:00
abstract::During sexual differentiation, Chlamydomonas reinhardtii changes its chemotactic behavior in response to ammonium. Just like gamete formation, the change in chemotaxis mode is controlled by the sequential action of two environmental cues, removal of ammonium or nitrate from the medium and light. Thus, vegetative cells...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1241-6
更新日期:2004-07-01 00:00:00
abstract::Plants of Pinguicula lusitanica were grown in individual Erlenmeyer flasks on an inorganic agar medium containing no nitrogen or phosphorus. After 8 weeks of culture, twenty of the plants were fed Pinus pollen 4 times over a period of 5 weeks.As a result of the feeding, the number of leaves as well as the diameters of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384859
更新日期:1967-03-01 00:00:00
abstract::Endogenous gibberellins were obtained in agar from the lower cut surface of upright sunflower shoot-tips. Exposure to unilateral light of the tips standing on agar, with the lower cut ends bisected by a vertical glass barrier at right angles to incident light, resulted in approximately 8 times the quantity of gibberel...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384773
更新日期:1972-12-01 00:00:00
abstract::The rate of glycolate excretion by Coccochloris peniocystis Kütz. cells incubated under conditions of low bicarbonate concentration and high light intensity was linear for only the initial 15 min of incubation and no additional glycolate accumulated in the medium after 20 min. Excretion was maximal in cells grown on 5...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393232
更新日期:1976-01-01 00:00:00
abstract::1. In Scenedesmus acutus Tomaselli, endogenous variations in cell progeny production and chlorophyll formation have been found which are very similar to those previously described in Chlorella by Hesse (Z. Pflanzenphysiol. 67, 58-77, 1972). When the dark phase of the light-dark-cycle is prolonged to a certain extent, ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388684
更新日期:1975-01-01 00:00:00
abstract::The concentration of cytoplasmic free calcium ([Ca(2+)](cyt)) required to close higher plant plasmodesmata was investigated using corn (Zea mays L. cv. Black Mexican Sweet) suspension-culture cells. Physiological elevations of [Ca(2+)](cyt) were applied by cold treatment, and ion injection was also used to increase [C...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/PL00008141
更新日期:2000-01-01 00:00:00
abstract::Callus cells of Daucus carota L. have different phenylpropanoid pathways depending on the medium composition. Cells propagated on a medium with gibberellic acid do not accumulate cyanidin but incorporate [(14)C]phenylalanine into chlorogenic acid at a high rate. Cells grown on a medium free of gibberellic acid accumul...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384906
更新日期:1977-01-01 00:00:00
abstract::The salt glands of Aegiceras corniculatum have been examined by light- and electron-microscope techniques. A gland consists of a large number of abutting secretory cells and a single, large basal cell. The secretory cells and basal cell are joined by well defined plasmodesmata. The glandular cuticle shows differences ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386836
更新日期:1971-09-01 00:00:00
abstract::Phosphate absorption by segments excised from hypocotyls of Phaseolus aureus Roxb. was inhibited by either continuous or short-term illumination with red light. Maximal inhibition was observed at pH 6.5. Illumination with 800 erg cm(-2) s(-1) of red light for 5 min was sufficient to obtain maximal inhibition. The effe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385271
更新日期:1975-01-01 00:00:00
abstract::Cutin and suberin polymers from various anatomical regions of grapefruit were analyzed chemically and ultrastructurally. The leaf, fruit peel and juice-sac showed an amorphous cuticular layer. The cutin in the leaf was composed of 10,16-dihydroxy C16 acid and its positional isomers as the major monomers whereas 16-hyd...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385755
更新日期:1980-10-01 00:00:00
abstract::Gibberellins A1, A8, A20 and A29 were identified by capillary gas chromatography-mass spectrometry in the pods and seeds from 5-d-old pollinated ovaries of pea (Pisum sativum cv. Alaska). These gibberellins were also identified in 4-d-old non-developing, parthenocarpic and pollinated ovaries. The level of gibberellin ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392389
更新日期:1987-01-01 00:00:00
abstract:MAIN CONCLUSION:A large-scale bioinformatics analysis revealed the origin and evolution of GT47 gene family, and identified two clades of intron-poor genes with putative functions in drought stress responses and seed development in maize. Glycosyltransferase family 47 (GT47) genes encode β-galactosyltransferases and β-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2821-6
更新日期:2018-03-01 00:00:00
abstract::The major fatty acid component of castor (Ricinus communis L.) oil is ricinoleic acid (12-hydroxy-cis-9-octadecenoic acid), and unsaturated hydroxy acid accounts for >85% of the total fatty acids in triacylglycerol (TAG). TAG had a higher ricinoleate content at position 2 than at positions 1 and 3. Although lysophosph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0083-1
更新日期:2006-01-01 00:00:00
abstract::Increasing soil salinity reduces crop yields worldwide, with rice being particularly affected. We have examined the correlation between apoplastic barrier formation in roots, Na+ uptake into shoots and plant survival for three rice (Oryza sativa L.) cultivars of varying salt sensitivity: the salt-tolerant Pokkali, mod...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0930-6
更新日期:2009-06-01 00:00:00
abstract::Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica napus L. AAPT1 gene (designated BnAAPT1) was identified from cDNA libraries of seedlings and developing se...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-1031-6
更新日期:2003-08-01 00:00:00
abstract::Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino acids through the expression of heterologous proteins have met with limite...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1487-8
更新日期:2012-01-01 00:00:00
abstract::In maize and other grasses there is a developmental gradient from the meristematic cells at the base of the stalk to the differentiated cells at the leaf tip. This gradient presents an opportunity to investigate changes in mitochondrial DNA (mtDNA) that accompany growth under light and dark conditions, as done previou...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1802-z
更新日期:2013-02-01 00:00:00
abstract::The α-xylosidase which is involved in the postgerminative mobilisation of xyloglucan in nasturtium seed cotyledons has now been purified to apparent homogeneity by a facile procedure involving lectin affinity chromatography. The purified enzyme, a glycoprotein, moved as a single band (apparent molecular weight 85000) ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00208247
更新日期:1991-04-01 00:00:00
abstract::The relationship between adenine-nucleotide levels and metabolism-dependent membrane potential was studied in cells of Nitellopsis obtusa. Effects of ADP and AMP in the presence of ATP on electrogenic pump activity were measured in the dark, using the continuous perfusion method. Both ADP and AMP acte as competitive i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397424
更新日期:1984-09-01 00:00:00
abstract::Light and temperature markedly influence sporangiophore development inPhycomyces blakesleeanus. Under normal conditions in the dark, low temperature drastically stimulates the production of dwarf sporangiophores (microphorogenesis) and inhibits that of giant sporangiophores (macrophorogenesis). These effects of low te...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00959514
更新日期:1988-06-01 00:00:00
abstract::Free porphyrins and their magnesium complexes, including chlorophylls, are potent photo-sensitizers. Plants usually accumulate these compounds bound to proteins together with protective compounds like carotenoids. Besides their protective role, carotenoids can play a structural role in these complexes. To analyze the ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0409-7
更新日期:2007-03-01 00:00:00
abstract::Three cDNAs (PgEMB22, 27 and 29) predicted to encode low-molecular-weight (LMW) heat-shock proteins (HSPs) were cloned and characterized from white spruce [Picea glauca (Moench) Voss] somatic embryo tissues by differentially screening a cotyledonary embryo cDNA library. Clone PgEMB22 is predicted to encode a putative ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00196653
更新日期:1996-01-01 00:00:00
abstract::A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1442-z
更新日期:2005-07-01 00:00:00
abstract::Six authentic gibberellins marginally promoted hypocotyl lengthening of etiolated lupin seedlings whereas extracted lupin "gibberellin-like" compounds were ineffective. However, dwarfing by AMO-1618 [2'-isopropyl-4'-(trimethyl-ammonium chloride)-5'-methylphenyl piperidine carboxylate] was totally counteracted by co-ap...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388394
更新日期:1974-09-01 00:00:00
abstract::Callose synthesis occurs at specific stages of plant cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We determined the expression pattern of "upstream regulatory sequence" of 12 Arabidopsis callose synthase genes (CalS1-12) genes and demonstrated that d...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0812-3
更新日期:2008-12-01 00:00:00
abstract::The nuclei of active, sieve cell-associated Strasburger cells in the secondary phloem of Pinus nigra var. austriaca (Hoess) Badoux have been studied for their structure and DNA and RNA content. No difference in size compared to those of ordinary ray cells was found. The nuclear surface is often increased by an ameboid...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394427
更新日期:1977-01-01 00:00:00
abstract:MAIN CONCLUSION:The research demonstrated that Arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. Arabidopsis has been established as an important model sy...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2237-5
更新日期:2015-04-01 00:00:00
abstract::Quinolizidine alkaloids formed in the leaves of Lupinus albus L. are translocated via the phloem to the other plant organs, especially the maturing fruits. Compared with amino-acid transport in the phloem, the alkaloids contribute about 8% to the overall nitrogen being exported from the leaf. Since it is likely that t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00407083
更新日期:1984-11-01 00:00:00
abstract::Cells of Nicotiana tabacum L. suspension cultures were treated with the respiratory inhibitor rotenone, which specifically inhibits complex I activity of mitochondria. Rotenone retarded cell growth, as shown by decreases in fresh weight, dry weight and cell numbers on a suspension-volume basis. However, rates of the c...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000457
更新日期:2001-04-01 00:00:00