Spatial and temporal changes in endogenous cytokinins in developing pea roots.

Abstract:

:Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Radicles were excised from sterile Pisum sativum L. seeds after 30 min and 5 h imbibition. Seedlings germinated on agar were harvested after 1, 3, 6 and 9 days. The roots were divided into the root tip, root free zone, secondary root zone and from day 6, the secondary roots. Samples were purified by various chromatographic methods and endogenous cytokinins detected by LC(+)ES-MS. Benzyladenine levels doubled after 5 h imbibition and then gradually decreased over time. Low concentrations of cis-Zeatin (cZ) type cytokinins were detected in the radicle after 30 min imbibition. After 5 h imbibition, cis-zeatin riboside-5'-monophosphate had greatly increased. The total cytokinin content of the roots increased over time with the ribotides being the predominant conjugates. From day 3 onwards, there was a gradual increase in the free bases, O-glucosides and their ribosylated forms. Mainly N ( 6 )-(2-isopentenyl)adenine (iP)-type cytokinins were detected in the root tip, whereas trans-zeatin- (tZ), dihyrozeatin- (DHZ) and iP-type cytokinins were found in the secondary roots and root zone. Cytokinin biosynthesis was only detected after day 6. Biosynthesis of iP and tZ derivatives was quite rapid, whereas biosynthesis of cZ derivatives remained at a low basal level. These fluctuations in cytokinin types and concentrations suggest the cytokinins may be synthesized from various pathways in pea roots.

journal_name

Planta

journal_title

Planta

authors

Stirk WA,Novák O,Václavíková K,Tarkowski P,Strnad M,van Staden J

doi

10.1007/s00425-008-0699-z

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

1279-89

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

227

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Rapid induction of ethylene biosynthesis in cultured parsley cells by fungal elicitor and its relationship to the induction of phenylalanine ammonia-lyase.

    abstract::The biosynthesis of ethylene was examined in suspension-cultured cells of parsley (Petroselinum hortense) treated with an elicitor from cell walls of Phytophthora megasperma. Untreated cells contained 50 nmol g(-1) of the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), and produced ethylene at a rate ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394581

    authors: Chappell J,Hahlbrock K,Boller T

    更新日期:1984-07-01 00:00:00

  • Proton excretion and cell expansion in bean leaves.

    abstract::Light-induced expansion of Phaseolus vulgaris L. leaf cells is accompanied by increased cell-wall plasticity. The possibility that leaf-cell walls are loosened by excreted protons has been investigated. First, light causes acidification, detected at the leaf surface, within 5-15 min. Growth starts 10-20 min after expo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00380038

    authors: Van Volkenburgh E,Cleland RE

    更新日期:1980-04-01 00:00:00

  • DBB1a, involved in gibberellin homeostasis, functions as a negative regulator of blue light-mediated hypocotyl elongation in Arabidopsis.

    abstract::Double B-box 1a (DBB1a) belongs to the zinc-finger family proteins in Arabidopsis thaliana. Transcriptional analysis uncovered that the DBB1a gene expression was blue light-dependently regulated, and the transcript level of DBB1a in cry1cry2 was decreased but not in phyAphyB compared to wild type under blue light cond...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1274-y

    authors: Wang Q,Zeng J,Deng K,Tu X,Zhao X,Tang D,Liu X

    更新日期:2011-01-01 00:00:00

  • An auxin-induced β-type endo-1,4-β-glucanase in poplar is involved in cell expansion and lateral root formation.

    abstract:MAIN CONCLUSION:PtrGH9A7, a poplar β-type endo-1,4-β-glucanase gene induced by auxin, promotes both plant growth and lateral root development by enhancing cell expansion. Endo-1,4-β-glucanase (EGase) family genes function in multiple aspects of plant growth and development. Our previous study found that PtrCel9A6, a po...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2851-8

    authors: Yu L,Li Q,Zhu Y,Afzal MS,Li L

    更新日期:2018-05-01 00:00:00

  • Formation of bound gibberellins inPharbitis nil.

    abstract::Developing seeds ofPharbitis nil accumulate free as well as bound gibberellins until a maximum level is reached at approximately 25 days after anthesis. Seeds from CCC-treated parent plants have a strongly reduced level of free as well as bound gibberellins. When different spray reagents were used it was found that tr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385821

    authors: Barendse GW

    更新日期:1971-12-01 00:00:00

  • An unusual effect of the far-red absorbing form of phytochrome: Photoinhibition of seed germination inBromus sterilis L.

    abstract::Seeds ofBromus sterilis L. germinated between 80-100% in darkness at 15° C but were inhibited by exposure to white or red light for 8 h per day. Exposure to far-red light resulted in germination similar to, or less than, that of seeds maintained in darkness. Germination is not permanently inhibited by light as seeds a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF01607578

    authors: Hilton JR

    更新日期:1982-11-01 00:00:00

  • Characterization of two phases of chlorophyll formation during greening of etiolated barley leaves.

    abstract::The esterification kinetics of chlorophyllide, obtained by a single flash of light, were investigated in etiolated barley ( Hordeum vulgare L.) and oat ( Avena sativa L.) leaves. A rapid phase, leading to esterification of 15% of total chlorophyllide within 15-30 s, was followed by a lag-phase of nearly 2 min and a su...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0870-x

    authors: Domanskii V,Rassadina V,Gus-Mayer S,Wanner G,Schoch S,Rüdiger W

    更新日期:2003-01-01 00:00:00

  • The induction of flowering in vitro in stem segments of Plumbago indica L. : I. The production of vegetative buds.

    abstract::Internode segments excised from vegetative stems of Plumbago indica, an absolute short-day plant, can be induced to form in vitro a callus, or roots, or vegetative shoots, or inflorescences and flowers. Callus formation was polarized when indolyl-3-acetic acid, L-tryptophane, naphthylacetamide, or 2,3-dichlorophenylac...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390146

    authors: Nitsch C,Nitsch JP

    更新日期:1967-12-01 00:00:00

  • Immuno-cytochemical localization of indole-3-acetic acid during induction of somatic embryogenesis in cultured sunflower embryos.

    abstract::Immature zygotic embryos of sunflower (Helianthus annuus L.) produce somatic embryos when cultured on medium supplemented with a cytokinin as the sole source of exogenous growth regulators. The timing of the induction phase and subsequent morphogenic events have been well characterized in previous work. We address her...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0791-8

    authors: Thomas C,Bronner R,Molinier J,Prinsen E,van Onckelen H,Hahne G

    更新日期:2002-08-01 00:00:00

  • Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat.

    abstract::Homeotic transformation of stamens into pistil-like structures (called pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum) having the cytoplasm of a wild relative species, Aegilops crassa. Our previous studies indicated that pistillody is caused by alteratio...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-007-0608-x

    authors: Saraike T,Shitsukawa N,Yamamoto Y,Hagita H,Iwasaki Y,Takumi S,Murai K

    更新日期:2007-12-01 00:00:00

  • Influence of gravity and light on the developmental polarity of Ceratopteris richardii fern spores.

    abstract::The polarity of germinating single-celled spores of the fern Ceratopteris richardii Brogn. is influenced by gravity during a time period prior to the first cellular division designated a "polarity-determination window". After this window closes, control of polarity is seen in the downward (with respect to gravity) mig...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050355

    authors: Edwards ES,Roux SJ

    更新日期:1998-08-01 00:00:00

  • Identification of microRNAs and their targets in tomato infected with Cucumber mosaic virus based on deep sequencing.

    abstract::MicroRNAs (miRNAs) play important regulatory roles in plant development and stress responses. Tomato is an economically important vegetable crop in the world with publicly available genomic information database, but only a limited number of tomato miRNAs have been identified. In this study, two independent small RNA l...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2158-3

    authors: Feng J,Liu S,Wang M,Lang Q,Jin C

    更新日期:2014-12-01 00:00:00

  • Organization of actin filaments in regenerating and outgrowing subprotoplasts from pollen tubes ofNicotiana tabacum L.

    abstract::The dynamics of actin-filament organization in pollen-tube subprotoplasts ofNicotiana tabacum L. cv. Samsun during regeneration and outgrowth was examined using phalloidin probes and a non-fixation method. A succession of actin arrays was examined during subprotoplast regeneration that strongly resembled the actin dyn...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02411443

    authors: Rutten TL,Derksen J

    更新日期:1990-03-01 00:00:00

  • Control by light of hypocotyl growth in de-etiolated mustard seedlings : II. Sensitivity for newly-formed phytochrome after a light to dark transtition.

    abstract::After sowing, mustard (Sinapis alba L.) seedlings were grown for 48 h in white light (25°C). These fully de-etiolated, green seedlings were used as experimental material between 48 and 72 h after sowing to study the control by phytochrome (Pfr) of hypocotyl elongation after a white light→dark transition. It was found ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387892

    authors: Wildermann A,Drumm H,Schäfer E,Mohr H

    更新日期:1978-01-01 00:00:00

  • Kinetics of intracellular redistribution of phytochrome in Avena coleoptiles after its photoconversion to the active, far-red-absorbing form.

    abstract::The kinetics of the intracellular redistribution of phytochrome (sequestering) in Avena sativa L. coleoptiles following a brief, saturating actinic pulse of red (R) light have been determined. Immunocytochemical labelling of phytochrome with monoclonal antibodies showed that at 22°C sequestering can occur within 1-2 s...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391335

    authors: McCurdy DW,Pratt LH

    更新日期:1986-03-01 00:00:00

  • Hydrogen evolution by several algae.

    abstract::Out of 33 strains of unicellular algae examined, H2 evolution was observed only in species of Chlamydomonas, Chlorella and Scenedesmus. While the photoevolution of H2 by these algae was generally stimulated both by an organic substrate and by the uncoupler CCCP(1), response to DCMU varied. On the basis of the response...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385481

    authors: Healey FP

    更新日期:1970-09-01 00:00:00

  • Localization of dibenzodioxocin substructures in lignifying Norway spruce xylem by transmission electron microscopy-immunogold labeling.

    abstract::The lignification process in mature Norway spruce [ Picea abies (L.) H. Karsten] xylem cell walls was studied using transmission electron microscopy (TEM)-immunogold detection with a polyclonal antibody raised against a specific lignin substructure, dibenzodioxocin. The study reveals for the first time the exact locat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-0983-x

    authors: Kukkola EM,Koutaniemi S,Gustafsson M,Karhunen P,Ruel K,Lundell TK,Saranpää P,Brunow G,Teeri TH,Fagerstedt KV

    更新日期:2003-06-01 00:00:00

  • Cytosolic cycles regulate the turnover of sucrose in heterotrophic cell-suspension cultures of Chenopodium rubrum L.

    abstract::We have investigated whether sucrose accumulation in heterotrophic cell-suspension cultures of Chenopodium rubrum L. is regulated by a cycle in which sucrose is simultaneously synthesised and degraded. Net sucrose accumulation was measured by monitoring the sucrose content, unidirectional synthesis was monitored by su...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197115

    authors: Dancer J,Hatzfeld WD,Stitt M

    更新日期:1990-09-01 00:00:00

  • Three-way interaction among plants, bacteria, and coleopteran insects.

    abstract:MAIN CONCLUSION:Coleoptera, the largest and the most diverse Insecta order, is characterized by multiple adaptations to plant feeding. Insect-associated microorganisms can be important mediators and modulators of interactions between insects and plants. Interactions between plants and insects are highly complex and inv...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-016-2543-1

    authors: Wielkopolan B,Obrępalska-Stęplowska A

    更新日期:2016-08-01 00:00:00

  • The influence of light, gibberellic acid and CCC on sprout growth and mobilization of tuber reserves in the potato (Solanum tuberosum L.).

    abstract::'Warm white' fluorescent light inhibited the elongation of potato sprouts and reduced the rate at which reserve materials in the mother tuber were mobilized. The evidence indicated that the influence of light on mobilization was independent of light effects on the magnitude of the 'sink' for translocates in the sprout...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385292

    authors: Morris DA

    更新日期:1967-09-01 00:00:00

  • Chronology of the differentiation of cotton (Gossypium hirsutum L.) fiber cells.

    abstract::Cotton fibers are single elongated cells that develop from epidermal cells of the ovule. The chronology of fiber differentiation was investigated using cultured ovules. Epidermal cells differentiate into fiber cells approx. 3 d before anthesis. When ovules were cultured on a defined medium, fiber growth could be initi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392435

    authors: Graves DA,Stewart JM

    更新日期:1988-08-01 00:00:00

  • Physiological elevations in cytoplasmic free calcium by cold or ion injection result in transient closure of higher plant plasmodesmata.

    abstract::The concentration of cytoplasmic free calcium ([Ca(2+)](cyt)) required to close higher plant plasmodesmata was investigated using corn (Zea mays L. cv. Black Mexican Sweet) suspension-culture cells. Physiological elevations of [Ca(2+)](cyt) were applied by cold treatment, and ion injection was also used to increase [C...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/PL00008141

    authors: Holdaway-Clarke TL,Walker NA,Hepler PK,Overall RL

    更新日期:2000-01-01 00:00:00

  • Influence of daylength on gibberellin metabolism and stem growth in Silene armeria.

    abstract::When radioactive gibberellin A5 ((3)H-GA5) was applied to the apices and surrounding young leaves of the long-day plant Silene armeria, it was partially converted to at least two other acidic substances. One of them was similar to GA3 in chromatographic, but not in biological properties. The other metabolite was more ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385349

    authors: van den Ende H,Zeevaart JA

    更新日期:1971-06-01 00:00:00

  • The functional significance of phloem anastomoses in stems ofDahlia pinnata Cav.

    abstract::The role of phloem anastomoses in translocation was studied experimentally in intact and wounded internodes ofDahlia pinnata Cav. Translocation was visualized with fluorescein, a fluorescent dye capable of movement in the phloem. Translocation was analyzed in large areas of living phloem tissue which were peeled from ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02341035

    authors: Aloni R,Peterson CA

    更新日期:1990-11-01 00:00:00

  • Occurence of nucleoside polyphosphates in rapidly labelled RNA preparations from radish seedlings (Raphanus sativus.).

    abstract::Preparations of rapidly labelled RNA are usually heavily contaminated by rapidly labelled low molecular weight components. This contaminating fraction mainly contains nucleoside triphosphates, as previously reported, but also nucleoside tetra, penta and hexaphosphates. These highly phosphorylated nucleosides are, acco...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385426

    authors: Aspart-Pascot L,Delseny M,Guitton Y

    更新日期:1976-01-01 00:00:00

  • Light-induced absorbance changes in Phycomyces: evidence for cryptochrome-associated flavosemiquinones.

    abstract::Light-induced absorbance changes (LIACs), which are associated with early photochemical events of blue-light transduction, were detected in growing zones of Phycomyces sporangiophores. The novel LIACs meet all the essential requirements for a spectrophotometric photoreceptor assay which was previously unavailable for ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050559

    authors: Schmidt W,Galland P

    更新日期:1999-04-01 00:00:00

  • 30-year progress of membrane transport in plants.

    abstract::In the past 30 years enormous progress was made in plant membrane biology and transport physiology, a fact reflected in the appearance of textbooks. The first book dedicated to 'Membrane Transport in Plants' was published on the occasion of the 'International Workshop on Membrane Transport in Plants' held at the Nucle...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-006-0341-x

    authors: Hedrich R,Marten I

    更新日期:2006-09-01 00:00:00

  • Effect of 2,4-dichlorophenoxyacetic acid on the structure and function of developing chloroplasts.

    abstract::Dark-grown radish seedlings (Raphanus sativus L.) were sprayed with 10(-3) mol·l(-1) 2,4-dichlorophenoxyacetic acid and then were exposed to a 14:10 light: dark cycle. Cotyledon samples from these seedlings and unsprayed controls were taken for electron microscopy, chlorophyll determinations, and photosynthetic rate m...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394437

    authors: Nadakavukaren MJ,McCracken DA

    更新日期:1977-01-01 00:00:00

  • Potassium permanganate fixative and the electron microscopy of sieve tube contents.

    abstract::Sieve tube "slime" is probably fibrillar as suggested by other workers. The granular material seen in electron micrographs of sieve tubes "fixed" with potassium permanganate is mostly a precipitate produced by the reaction of the permanganate with the sieve tube contents, particularly with sucrose, and with reagents u...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385369

    authors: Johnson RP

    更新日期:1966-03-01 00:00:00

  • A comparative study of the responsivity of Sinapis alba L. seedlings to pulsed and continuous irradiation.

    abstract::Anthocyanin formation in 36h dark grown Sinapis alba L. seedlings and inhibition of hypocotyl elongation in 36h and 54h dark grown and 54h and 7 day light grown seedlings in response to continuous red light could be substituted for by hourly 5 min light pulses where the total fluence over the irradiation period is the...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00383896

    authors: Schäfer E,Beggs CJ,Fukshansky L,Holmes MG,Jabben M

    更新日期:1981-11-01 00:00:00