Potassium permanganate fixative and the electron microscopy of sieve tube contents.

Abstract:

:Sieve tube "slime" is probably fibrillar as suggested by other workers. The granular material seen in electron micrographs of sieve tubes "fixed" with potassium permanganate is mostly a precipitate produced by the reaction of the permanganate with the sieve tube contents, particularly with sucrose, and with reagents used in the fixing, dehydrating and embedding process. Potassium permanganate is not therefore a good fixative for the electron microscopy of sieve tube contents. Neither is it suitable for the study of fine structure in vacuoles or vesicles containing precipitable solutes in other kinds of cells nor for examining the fine structure of their ground cytoplasm.

journal_name

Planta

journal_title

Planta

authors

Johnson RP

doi

10.1007/BF00385369

subject

Has Abstract

pub_date

1966-03-01 00:00:00

pages

36-43

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

68

pub_type

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