Abstract:
:A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8x10(-9) M, typical of known peptide hormone-receptor interactions in both plants and animals. The (125)I-azido-LeRALF, when exposed to UVB light in the presence of the cells, strongly labeled only two proteins of 25 kDa and 120 kDa, with the 25 kDa protein being more strongly labeled than the 120 kDa protein. The cell-surface localization of the interaction was indicated, as suramin, a known inhibitor of peptide-receptor interactions, and native LeRALF peptide competed with (125)I-azido-LeRALF labeling of both proteins. Two biologically inactive LeRALF analogs were not competitors. Incubation of (125)I-azido-LeRALF with suspension cultured cells in the dark, where it was fully active, could subsequently be totally dissociated from cells by acid washes, indicating that it was interacting at the cell surface and was not internalized. The (125)I-azido-LeRALF-labeled 25 kDa and 120 kDa proteins could not be solubilized from cell membranes by methods that release peripheral proteins, indicating that they are integral membrane components. The cumulative kinetic and biochemical evidence strongly indicates that the two proteins may be components of a LeRALF receptor complex.
journal_name
Plantajournal_title
Plantaauthors
Scheer JM,Pearce G,Ryan CAdoi
10.1007/s00425-004-1442-zsubject
Has Abstractpub_date
2005-07-01 00:00:00pages
667-74issue
5eissn
0032-0935issn
1432-2048journal_volume
221pub_type
杂志文章相关文献
PLANTA文献大全abstract::A 5-methyltryptophan(5-MT)-resistant cell line of wild carrot (Daucus carota L.), W001, that exhibited auxin-independent callus growth, was found to accumulate indole-3-acetic acid (IAA) and tryptophan (trp). Anthranilate-synthetase activity in W001 cell extract was less sensitive to feedback inhibition by trp than in...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388358
更新日期:1979-01-01 00:00:00
abstract::The uptake of sulphate into roots of barley seedlings is highly sensitive to phenylglyoxal (PhG), an arginine-binding reagent. Uptake was inhibited by >80% by a 1-h pre-treatment of roots with 0.45 mol · m(-3) PhG. Inhibition was maximal in pre-treatment solutions buffered between pH 4.5 and 6.5. Phosphate uptake, mea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195653
更新日期:1992-06-01 00:00:00
abstract::Plant root development is highly responsive both to changes in nitrate availability and beneficial microorganisms in the rhizosphere. We previously showed that Phyllobacterium brassicacearum STM196, a plant growth-promoting rhizobacteria strain isolated from rapeseed roots, alleviates the inhibition exerted by high ni...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-010-1264-0
更新日期:2010-11-01 00:00:00
abstract::The adaxial leaf cuticle of Malus pumila was examined by electron microscopy to determine possible avenues for transcuticular movement of foliarly applied chemicals. Cutin-embedded polysaccharide microfibrils originated at the outer epidermal cell wall and occasionally extended to the cuticle surface. Lamellae, ca. 4 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388736
更新日期:1979-12-01 00:00:00
abstract::Ten-cell-long filaments of the caulonema of Funaria hygrometrica were isolated and labeled with (3)H-thymidine. During the process of regeneration this precursor is incorporated into the nucleus and the chloroplasts. The nuclei of aged cells are preferentially labeled, even the nuclei of such cells which probably will...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385072
更新日期:1969-09-01 00:00:00
abstract::Detached phyllodia ofBryophyllum tubiflorum were fed under illumination with(14)CO2 at different times during the light/dark period (12:12 hours). After photosynthesis in presence of(14)CO2 during the intrinsic dark period the greatest part of soluble radioactivity was found in malate. When the same experiment was rep...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF01391117
更新日期:1969-06-01 00:00:00
abstract::Gibberellic-acid(GA3)-induced α-amylase has been localised in barley aleurone layers using cytochemical methods and light microscopy. Evidence obtained from the use of a starch substrate film method as well as immunofluorescence indicated that the first amylase to appear in the cell was associated with aleurone grains...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385325
更新日期:1973-09-01 00:00:00
abstract::The electrochemical potential differences [Formula: see text] for potassium, between excised barley (Hordeum vulgare L.) roots and external media containing 0.05 mM KCl+0.5 mM CaSO4, were determined over a 4-h period during which initially low-K(+) roots accumulated K(+) by pretreatment in 50 mM KCl plus 0.5 mM CaCl2....
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388368
更新日期:1979-01-01 00:00:00
abstract::Water and solute relations of excised seminal roots of young maize (Zea mays L) plants, have been measured using the root pressure probe. Upon addition of osmotic solutes to the root medium, biphasic root pressure relaxations were obtained as theoretically expected. The relaxations yielded the hydraulic conductivity L...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00403585
更新日期:1989-03-01 00:00:00
abstract:MAIN CONCLUSION:Endogenous auxin determines the pattern of adventitious shoot formation. Auxin produced in the dominant shoot is transported to the internodal segment and suppresses growth of other shoots. Adventitious shoot formation is required for the propagation of economically important crops and for the regenerat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03367-5
更新日期:2020-03-05 00:00:00
abstract:MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2431-0
更新日期:2016-03-01 00:00:00
abstract:MAIN CONCLUSION:Expression of bHLH104 - GFP driven by the MYB72 promoter improves plants' tolerance to Fe deficiency and increases seed Fe concentrations. Iron (Fe) deficiency causes reduced crop yield and quality. In humans, Fe deficiency is directly associated with Fe-deficiency anemia. Therefore, breeding Fe-deficie...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2703-y
更新日期:2017-09-01 00:00:00
abstract::Endogenous extractable factors associated with auxin action in plant tissues were investigated, especially their effects on elongation of 1-mm coleoptile segments of maize (Zea mays L.), in the presence of saturating 10 microM indole-3-acetic acid (IAA). The relative growth response, to auxin alone, was much smaller i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000470
更新日期:2001-05-01 00:00:00
abstract::Plants are exposed to a wide range of toxic and bioactive low-molecular-weight molecules from both exogenous and endogenous sources. Glycosylation is one of the primary sedative mechanisms that plants utilise in order to maintain metabolic homeostasis. Recently, a range of glycosyltransferases has been characterized i...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s004250000492
更新日期:2001-06-01 00:00:00
abstract::Rapid changes in gene expression were studied during incubation of cucumber (Cucumis sativus L.) cotyledons with cytokinins in darkness. Complementary-DNA clones for mRNAs whose levels decreased within 4 h of treatment with N6-benzyladenine (BA) were isolated by differential hybridization. One of them (CR9) was sequen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF02411564
更新日期:1994-01-01 00:00:00
abstract::MicroRNAs (miRNAs) play important regulatory roles in plant development and stress responses. Tomato is an economically important vegetable crop in the world with publicly available genomic information database, but only a limited number of tomato miRNAs have been identified. In this study, two independent small RNA l...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2158-3
更新日期:2014-12-01 00:00:00
abstract::Throughout a period of 23 days, during which the photosynthesis, growth and transpiration of two species of Atriplex were compared, A. spongiosa, a "C4" species (first products of photosynthesis = 4-C dicarboxylic acids), maintained net rates of leaf photosynthesis as high as, or higher than, those of A. hastata, a "C...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387639
更新日期:1970-09-01 00:00:00
abstract::Sugar-synthesis and -transport patterns were analyzed in Coleus blumei Benth. leaves to determine where galactinol, raffinose, and stachyose are made and whether phloem loading includes an apoplastic (extracellular) step or occurs entirely within the symplast (plasmodesmata-connected cytoplasm). To clarify the sequenc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195663
更新日期:1992-06-01 00:00:00
abstract::1. The course of senescence in leaves of corn seedlings, as measured by the loss of chlorophyll, is shown to be controlled by two correlative influences: a) In the isolated leaf, the base tends to enhance yellowing of the tip region. b) In the leaf attached to the intact plant, correlative aging is delayed by interact...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385624
更新日期:1966-06-01 00:00:00
abstract::In order to better understand the various pathways of sucrose and starch catabolism in the anther of lily (Lilium hybrida var. "Enchantment"), invertase (EC 3.2.1.26) and amylase (EC 3.2.1.1, EC 3.2.1.2) activities were measured separately in different fractions (anther wall, locular fluid and microspore/pollen) and c...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0443-5
更新日期:2007-05-01 00:00:00
abstract::Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ab...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00401030
更新日期:1988-03-01 00:00:00
abstract::The major storage proteins isolated from wild-type seeds of Arabidopsis thaliana (L.) Heynh., strain "Columbia", were studied by sucrose gradient centrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Both the hypocotyl and cotyledons of mature embryos contained abundant 12 S (crucife...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392124
更新日期:1986-11-01 00:00:00
abstract::Monoclonal antibodies have been raised against haustorial complexes isolated from pea (Pisum sativum L.) leaves infected by the biotrophic powdery mildew fungus Erysiphe pisi D.C. Immuno-localisation studies, using isolated haustorial complexes and infected pea leaf material, have shown that one of the antibodies, des...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197739
更新日期:1991-02-01 00:00:00
abstract::A group of frequent cDNA clones from a young-leaf cDNA library was found to code for a homologue of S-ribonucleases (S-RNases) involved in gametophytic incompatibility and the so-called S-like RNases active in flowers and in vegetative tissues. The derived amino acid sequence starts with a signal peptide and has a 27-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050046
更新日期:2000-03-01 00:00:00
abstract:MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03435-w
更新日期:2020-08-01 00:00:00
abstract::Ricinus communis cv. Carmencita seedlings with their cotyledons incubated in sucrose solution and their hypocotyls cut to induce exudation of phloem sap, constitute a system of sucrose fluxes into and out of the cotyledons. This system was characterized with respect to quasi-steady-state conditions of sucrose uptake a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00199964
更新日期:1992-07-01 00:00:00
abstract::Limbs of flower buds from Petunia hybrida were investigated for β-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferyl-β-D-glucoside as substrates. Dihydroflavonol-glucoside β-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active toward 4-methyl-um...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392547
更新日期:1982-07-01 00:00:00
abstract::The maximum extractable activities of twenty-one photosynthetic and glycolytic enzymes were measured in mature leaves of Mesembryanthemum crystallinum plants, grown under a 12 h light 12 h dark photoperiod, exhibiting photosynthetic characteristics of either a C3 or a Crassulacean acid metabolism (CAM) plant. Followin...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00402925
更新日期:1982-06-01 00:00:00
abstract::A rice htd-1 mutant, related to tillering and dwarfing, was characterized. We show that the htd-1 mutant increases its tiller number by releasing axillary buds from dormant stage rather than by initiating more axillary buds. The dwarf is caused by averagely reducing each internode and panicle. Based on this dwarfing p...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0007-0
更新日期:2005-11-01 00:00:00
abstract::In several plant species, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase consists of two isoforms that are produced by alternative splicing of a pre-mRNA. Two forms of activase corresponding to the longer, redox-regulated alpha and the shorter, beta forms were detected immunologically in cotton (Go...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0923-1
更新日期:2003-03-01 00:00:00