Cell wall localization of dihydroflavonol-glucoside β-glucosidase in flowers of Petunia hybrida.

Abstract:

:Limbs of flower buds from Petunia hybrida were investigated for β-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferyl-β-D-glucoside as substrates. Dihydroflavonol-glucoside β-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active toward 4-methyl-umbelliferyl-β-glucoside. Besides this activity a neutral β-glucosidase is present. This activity is soluble and is not active toward dihydroflavonol-glucosides. Using starch gel electrophoresis it was shown that no difference in β-glucosidase activity is present between mutants able to convert dihydroflavonols into anthocyanins and mutants accumulating dihydroflavonol-glucosides. It is concluded that β-glucosidase activity is not involved in anthocyanin synthesis.

journal_name

Planta

journal_title

Planta

authors

Schram AW,Al EJ,Douma N,Jonsson LM,de Vlaming P,Kooi A,Bennink GJ

doi

10.1007/BF00392547

subject

Has Abstract

pub_date

1982-07-01 00:00:00

pages

162-5

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

155

pub_type

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