Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver.

Abstract:

:Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.

journal_name

Planta

journal_title

Planta

authors

Suzuki N,Uefuji H,Nishikawa T,Mukai Y,Yamashita A,Hattori M,Ogasawara N,Bamba T,Fukusaki E,Kobayashi A,Ogata Y,Sakurai N,Suzuki H,Shibata D,Nakazawa Y

doi

10.1007/s00425-012-1679-x

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

1405-17

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

236

pub_type

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