Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus × domestica) results in short-day induced dormancy and increased cold hardiness.

Abstract:

:Low, non-freezing temperatures and/or short daylength (SD) regulates cold acclimation and dormancy in fruit trees. Regarding cold acclimation, C-repeat binding factor (CBF/DREB) transcriptional activator genes have the well-documented ability to induce the expression of a suite of genes associated with increased cold tolerance. We isolated a full-length cDNA of a peach CBF gene, designated PpCBF1 (GenBank Accession HM992943), and constitutively expressed it using an enhanced 35S promoter in apple. Unexpectedly, constitutive overexpression of the PpCBF1 in apple resulted in strong sensitivity to short daylength. Growth cessation and leaf senescence were induced in transgenic lines exposed to SD and optimal growth temperatures of 25°C over a 4-week period. Following 1-4 weeks of SD and 25°C trees were returned to LD and 25°C in the greenhouse. Control (untransformed) plants continued to grow while transgenic lines receiving two or more weeks of SD remained dormant and began to drop leaves. Constitutive overexpression of the PpCBF1 in apple resulted in a 4-6°C increase in freezing tolerance in both the non-acclimated and acclimated states, respectively, compared with untransformed M.26 trees. This is the first instance that constitutive overexpression of a CBF gene has resulted in SD-induction of dormancy and to our knowledge the first time apple has been shown to strongly respond to short daylength as a result of the insertion of a transgene.

journal_name

Planta

journal_title

Planta

authors

Wisniewski M,Norelli J,Bassett C,Artlip T,Macarisin D

doi

10.1007/s00425-011-1358-3

subject

Has Abstract

pub_date

2011-05-01 00:00:00

pages

971-83

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

233

pub_type

杂志文章

相关文献

PLANTA文献大全
  • High levels of asparagine synthetase in hypocotyls of pine seedlings suggest a role of the enzyme in re-allocation of seed-stored nitrogen.

    abstract::A pine asparagine synthetase gene expressed in developing seedlings has been identified by cloning its cDNA (PsAS1) from Scots pine (Pinus sylvestris L.). Genomic DNA analysis with PsAS1 probes and a sequence-based phylogenetic tree are consistent with the possibility of more than one gene encoding asparagine syntheta...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0196-6

    authors: Cañas RA,de la Torre F,Cánovas FM,Cantón FR

    更新日期:2006-06-01 00:00:00

  • Biological specificity of the antheridogen. from Ceratopteris thalictroides (L.) Brongn.

    abstract::The relationship of the antheridogen from Ceratopteris thalictroides (L.) Brongn. (Antheridogen C) to three other known antheridogens, gibberellic acid, and two natural antheridogens, one from Pteridium aquilinum (Antheridogen A) and one from Anemia phyllitidis (Antheridogen B), is investigated. It is shown that each ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390201

    authors: Schedlbauer MD

    更新日期:1974-03-01 00:00:00

  • Evidence for a uridine-5'-diphosphate-glucose-protectedp-chloromercuribenzene sulfonic acid-binding site in sugarcane vacuoles.

    abstract::The uptake of uridine-5'-diphosphate (UDP) glucose into vacuoles isolated fromSaccharum sp. cells was fully inhibited by pretreatment with 50 μMp-chloromercuribenzenesulfonic acid (PCMBS) and was not affected by N-ethylmaleimide up to a concentration of 5 mM. The addition of 10 mM UDP-glucose during the pretreatment p...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF01369776

    authors: Delrot S,Thom M,Maretzki A

    更新日期:1986-03-01 00:00:00

  • Composition and content of glucosinolates in developing Arabidopsis thaliana.

    abstract::The glucosinolate composition and content in various tissues of Arabidopsis thaliana (L.) Heynh. ecotype Columbia during development from seeds to bolting plants were determined in detail by high-performance liquid chromatography. Comparison of the glucosinolate profiles of leaves, roots and stems from mature plants w...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100659

    authors: Petersen BL,Chen S,Hansen CH,Olsen CE,Halkier BA

    更新日期:2002-02-01 00:00:00

  • Ubiquitous presence of beta-glucuronidase (GUS) in plants and its regulation in some model plants.

    abstract::The enzyme beta-glucuronidase (GUS) is well characterized in animals and microbes. However, this enzyme is not well studied in plants and is widely assumed to be absent in them. In this study we document the ubiquitous presence of GUS in the model plants Arabidopsis thaliana, Oryza sativa, Nicotiana tabacum and Zea ma...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0276-2

    authors: Sudan C,Prakash S,Bhomkar P,Jain S,Bhalla-Sarin N

    更新日期:2006-09-01 00:00:00

  • Actin-related defense mechanism to reject penetration attempt by a non-pathogen is maintained in tobacco BY-2 cells.

    abstract::The actin cytoskeleton is a key player in defense responses during early stages of infection by fungal pathogens. To investigate molecular mechanisms of actin-related defense responses, a cultured tobacco ( Nicotiana tabacum L.) BY-2 cell system was devised. When conidia were directly deposited on BY-2 cells, neither ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1042-3

    authors: Kobayashi I,Hakuno H

    更新日期:2003-06-01 00:00:00

  • Synthesis and oxidative insolubilization of cell-wall proteins during osmotic stress.

    abstract::The cell walls in the new white roots of jack pine (Pinus banksiana Lamb.) were observed to constrict around the shrinking protoplast of osmotically stressed roots, and pressure was maintained via an apparent adjustment of cell-wall size and elasticity. These elastic alterations of the cell wall permitted the root cel...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050575

    authors: Marshall JG,Dumbroff EB,Thatcher BJ,Martin B,Rutledge RG,Blumwald E

    更新日期:1999-05-01 00:00:00

  • Mode of glucan degradation by purified phosphorylase forms from spinach leaves.

    abstract::The glucan specifity of the purified chloroplast and non-chloroplast forms of α-1,4-glucan phosphorylase (EC 2.4.1.1) from spinach leaves (Steup and E. Latzko (1979), Planta 145, 69-75) was investigated. Phosphorolysis by the two enzymes was studied using a series of linear maltodextrins (degree of polymerization ≦11)...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384254

    authors: Steup M,Schächtele C

    更新日期:1981-12-01 00:00:00

  • Mapping QTLs for improving grain yield using the USDA rice mini-core collection.

    abstract::Yield is the most important and complex trait for genetic improvement in crops, and marker-assisted selection enhances the improvement efficiency. The USDA rice mini-core collection derived from over 18,000 accessions of global origins is an ideal panel for association mapping. We phenotyped 203 O. sativa accessions f...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1405-0

    authors: Li X,Yan W,Agrama H,Jia L,Shen X,Jackson A,Moldenhauer K,Yeater K,McClung A,Wu D

    更新日期:2011-08-01 00:00:00

  • Tropisms in Phycomyces: sine law for gravitropism, exponential law for photogravitropic equilibrium.

    abstract::Sporangiophores of Phycomyces blakesleeanus that are gravitropically stimulated by inclining them relative to the earth's gravitational vector obey the sine law for inclination angles between 0 degrees and 150 degrees. The quantitative relation between gravitropism and phototropism was analyzed for sporangiophores tha...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-001-0705-1

    authors: Galland P,Wallacher Y,Finger H,Hannappel M,Tröster S,Bold E,Grolig F

    更新日期:2002-04-01 00:00:00

  • Stimulation of precursor rRNA synthesis in the cotyledons of mustard seedlings by phytochrome.

    abstract::Cotyledons of mustard (Sinapis alba L.) seedlings irradiated with far-red light were shown to synthesize high molecular weight precursor rRNA (2.4×10(6)-2.9×10(6) dalton) at a considerably enhanced rate compared to dark-grown cotyledons. This result was obtained by pulse-labelling cotyledons isolated from 48 h old see...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384765

    authors: Thien W,Schopfer P

    更新日期:1975-01-01 00:00:00

  • [Growth of green suspension cultures of nicotiana tabacum var. "Samsun" with CO2 as carbon source].

    abstract::The photosynthetic CO2-fixation of cell cultures of Nicotiana tabacum var. "Samsun" is strongly inhibited by 2,4-dichlorphenoxyacetic acid and lies below the compensation point in cells grown with 2,4-D as an auxin. Cultivating the tobacco cells with α-naphthylacetic acid instead of 2,4-D results in an increase in the...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384845

    authors: Bergmann L

    更新日期:1967-09-01 00:00:00

  • Comparative photosynthesis, growth and transpiration of two species of Atriplex.

    abstract::Throughout a period of 23 days, during which the photosynthesis, growth and transpiration of two species of Atriplex were compared, A. spongiosa, a "C4" species (first products of photosynthesis = 4-C dicarboxylic acids), maintained net rates of leaf photosynthesis as high as, or higher than, those of A. hastata, a "C...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387639

    authors: Slatyer RO

    更新日期:1970-09-01 00:00:00

  • Rapid increase of vacuolar volume in response to salt stress.

    abstract::Suspension-cultured cells of mangrove [Bruguiera sexangula (Lour.) Poir.] showed a rapid increase in vacuolar volume under salt stress, although there was no change in the cell volume. The rapid increase in the vacuolar volume was an active process, which followed the activation of the tonoplast H(+)-ATPase and the va...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0878-2

    authors: Mimura T,Kura-Hotta M,Tsujimura T,Ohnishi M,Miura M,Okazaki Y,Mimura M,Maeshima M,Washitani-Nemoto S

    更新日期:2003-01-01 00:00:00

  • Topochemical and morphological characterization of wood cell wall treated with the ionic liquid, 1-ethylpyridinium bromide.

    abstract::MAIN CONCLUSION : [EtPy][Br] is more reactive toward lignin than toward the PSs in wood cell walls, and [EtPy][Br] treatment results in inhomogenous changes to the cell wall's ultrastructural and chemical components. The effects of the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]), which prefers to react with li...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2235-7

    authors: Kanbayashi T,Miyafuji H

    更新日期:2015-09-01 00:00:00

  • Changes in the phenolic metabolism of suspension cultures of Acer pseudoplatanus L. Caused by the addition of 2-(chloroethyl)phosphonic acid (CEPA).

    abstract::2-(Chloroethyl)phosphonic acid (CEPA) inhibited the rise in tannin production and phenylalanine ammonia-lyase EC 4.1.1.5 (PAL) activity shown by Acer cells in media containing 9.0×10(-7) M 2,4-D for a period of 2-3 days after its addtion. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385416

    authors: Westcott RJ

    更新日期:1976-01-01 00:00:00

  • C4-Pathway photosynthesis in Portulaca oleracea and the significance of alanine labelling.

    abstract::Portulaca oleracea L. has some features common to C4 species but it has been suggested from other studies that a substantial proportion of assimilated carbon dioxide is incorporated into pyruvate and alanine by routes not involving C4 acids or 3-phosphoglycerate. The present paper reports that enzyme activities in P. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385603

    authors: Hatch MD

    更新日期:1975-01-01 00:00:00

  • Gene expression in the soybean seed axis during germination and early seedling growth.

    abstract::Copy-DNA clones have been obtained that distinguish eight messenger mRNAs, moderately abundant in the axes of the germinating soybean (Glycine max (L.) Merr.) seedling. These clones have been used to characterize the size of the mRNAs and to anlyze the accumulation of the mRNAs at different time points and in differen...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397890

    authors: Datta K,Parker H,Averyhart-Fullard V,Schmidt A,Marcus A

    更新日期:1987-02-01 00:00:00

  • Spectrophotometric phytochrome measurements in light-grown Avena sativa L.

    abstract::Phytochrome was studied spectrophotometrically in Avena sativa L. seedlings that had been grown for 6 d in continous white fluorescent light from lamps. Greening was prevented through the use of the herbicide San 9789. When placed in the light, phytochrome (Ptot) decreased with first order kinetics (τ1/2 ≈ 2 h) but re...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392003

    authors: Jabben M,Deitzer GF

    更新日期:1978-01-01 00:00:00

  • Dithiothreitol, an inhibitor of violaxanthin de-epoxidation, increases the susceptibility of leaves ofNerium oleander L. to photoinhibition of photosynthesis.

    abstract::Leaves ofNerium oleander L. plants, which had been previously kept in a shaded glasshouse for at least two months, were fed 1 mM dithiothreitol (DTT) through their petioles, either for 12h in darkness (overnight) or for 2h in low light (28 μmol photons·m(-2)·s(-1)), in each case followed by a 3-h exposure to high ligh...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02411406

    authors: Winter K,Königer M

    更新日期:1989-12-01 00:00:00

  • Tuning of pectin methylesterification: consequences for cell wall biomechanics and development.

    abstract:MAIN CONCLUSION:Recent publications have increased our knowledge of how pectin composition and the degree of homogalacturonan methylesterification impact the biochemical and biomechanical properties of plant cell walls, plant development, and plants' interactions with their abiotic and biotic environments. Experimental...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-015-2358-5

    authors: Levesque-Tremblay G,Pelloux J,Braybrook SA,Müller K

    更新日期:2015-10-01 00:00:00

  • Engineering flavonoid glycosyltransferases for enhanced catalytic efficiency and extended sugar-donor selectivity.

    abstract::Flavonoids are predominantly found as glycosides in plants. The glycosylation of flavonoids is mediated by uridine diphosphate-dependent glycosyltransferases (UGT). UGTs attach various sugars, including arabinose, glucose, galactose, xylose, and glucuronic acid, to flavonoid aglycones. Two UGTs isolated from Arabidops...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1922-0

    authors: Kim HS,Kim BG,Sung S,Kim M,Mok H,Chong Y,Ahn JH

    更新日期:2013-10-01 00:00:00

  • Lipid metabolism in green leaves of developing monocotyledons.

    abstract::Lipid synthesis was studied in successive leaf sections from the base to the tip of developing barley (Hordeum vulgare L.), maize (Zea mays L.), rye grass (Lolium perenne L.) and wheat (Triticum aestivum L.) leaves. The endogenous levels of acyl lipids and their constituent fatty acids from the same leaf sections were...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388640

    authors: Bolton P,Harwood JL

    更新日期:1978-01-01 00:00:00

  • Increased root oxygen uptake in pea plants responding to non-self neighbors.

    abstract::Recent studies have demonstrated that plants alter root growth and decrease competition with roots of the same individual (self); however, the physiological traits accompanying this response are still widely unknown. In this study, we investigated the effect of root identity on gas exchange in the model species pea (P...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1910-4

    authors: Meier IC,Angert A,Falik O,Shelef O,Rachmilevitch S

    更新日期:2013-09-01 00:00:00

  • A 1,4-β-D-glucan-synthase system from Dictyostelium discoideum.

    abstract::Particulate membrane preparations have been isolated from culminating Dictyostelium discoideum cells. The preparations incorporated glucose from uridine 5'-diphosphate-glucose into a glucose polymer or polymers. These have been shown to be homopolymers of β-linked glucose. A high percentage (78% by methylation analysi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00198783

    authors: Blanton RL,Northcote DH

    更新日期:1990-02-01 00:00:00

  • Soluble and microsomal glutatione S-transferase activities in pea seedlings (Pisum sativum L.).

    abstract::Epicotyl and primary leaves of pea seedlings (Pisum sativum L., var. Alaska) were found to contain soluble and microsomal enzymes catalyzing the addition of glutathione to the olefinic double bond of cinnamic acid. Glutathione S-cinnamoyl transfer was also obtained with enzyme preparations from potato slices and cell ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388845

    authors: Diesperger H,Sandermann H Jr

    更新日期:1979-10-01 00:00:00

  • Fungal and plant gene expression during the colonization of cacao seedlings by endophytic isolates of four Trichoderma species.

    abstract::Endophytic isolates of Trichoderma species are being considered as biocontrol agents for diseases of Theobroma cacao (cacao). Gene expression was studied during the interaction between cacao seedlings and four endophytic Trichoderma isolates, T. ovalisporum-DIS 70a, T. hamatum-DIS 219b, T. harzianum-DIS 219f, and Tric...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0314-0

    authors: Bailey BA,Bae H,Strem MD,Roberts DP,Thomas SE,Crozier J,Samuels GJ,Choi IY,Holmes KA

    更新日期:2006-11-01 00:00:00

  • Production of extracellular fibers by tobacco leaf epidermal protoplasts.

    abstract::Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca(2+) in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397537

    authors: Fannin FF,Shaw JG

    更新日期:1983-11-01 00:00:00

  • The extrinsic proteins of photosystem II: update.

    abstract:MAIN CONCLUSION:Recent investigations have provided important new insights into the structures and functions of the extrinsic proteins of Photosystem II. This review is an update of the last major review on the extrinsic proteins of Photosystem II (Bricker et al., Biochemistry 31:4623-4628 2012). In this report, we wil...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-015-2462-6

    authors: Roose JL,Frankel LK,Mummadisetti MP,Bricker TM

    更新日期:2016-04-01 00:00:00

  • Endogenous gibberellin transport and biosynthesis in relation to geotropic induction of excised sunflower shoot-tips.

    abstract::Surgical experiments on Helianthus annuus and Phaseolus multiflorus seedlings involving the application of auxin and gibberellin to decapitated plants, suggested that internode extension growth occurs under the controlling influence of apically synthesised gibberellin rather than auxin. Studies were made of diffusible...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385395

    authors: Phillips ID

    更新日期:1972-09-01 00:00:00