Abstract:
:The enzyme beta-glucuronidase (GUS) is well characterized in animals and microbes. However, this enzyme is not well studied in plants and is widely assumed to be absent in them. In this study we document the ubiquitous presence of GUS in the model plants Arabidopsis thaliana, Oryza sativa, Nicotiana tabacum and Zea mays and record its expression pattern. The pH of the assay buffer was found to be critical with pH 4.0 being optimum for detection in all the species. GUS in plants appears to be associated with growth. In general, younger regions of the organs showed more GUS activity than the older and more mature tissues. In Brassica juncea roots stained for GUS, intense blue color could be seen in the trichoblast cells and the growing root hair cells as compared to the non-root hair forming epidermal cells or the fully elongated root hairs. Cotton fibers showed high GUS activity during the initial phase of elongation while the seed coat, from which the fibers formed, did not stain for GUS activity. The activity in the fibers disappeared after they were fully elongated. The level of GUS activity increased 2.58 folds in leaf tissues of N. tabacum when cultured in MS medium supplemented with 6-benzylaminopurine, while gibberellic acid enhanced GUS activity 2.9 folds in the inter-nodal regions of rice in 12-h treatment. In addition, elongation of stem, root and root hairs in tobacco seedlings was strongly inhibited by the specific inhibitor of GUS, saccharo-1-4-lactone in a reversible manner. Taken together, these evidences suggest a probable association of plant GUS in cell growth.
journal_name
Plantajournal_title
Plantaauthors
Sudan C,Prakash S,Bhomkar P,Jain S,Bhalla-Sarin Ndoi
10.1007/s00425-006-0276-2subject
Has Abstractpub_date
2006-09-01 00:00:00pages
853-64issue
4eissn
0032-0935issn
1432-2048journal_volume
224pub_type
杂志文章相关文献
PLANTA文献大全abstract::Glutathione-dependent formaldehyde dehydrogenase (FDH; EC 1.2.1.1) has been purified 3900-fold from maize cell-suspension cultures to a specific activity of 4.68 mumol (mg protein)-1 min-1. The homogeneous enzyme consisted of two identical subunits with a molecular mass of 42 kDa, and an isoelectric point of 5.8. Eigh...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050529
更新日期:1999-03-01 00:00:00
abstract:MAIN CONCLUSION:Formation of specific ultrastructural chromoplastidal elements during ripening of fruits of three different colored Physalis spp. is closely related to their distinct carotenoid profiles. The accumulation of color-determining carotenoids within the chromoplasts of ripening yellow, orange, and red fruit ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03383-5
更新日期:2020-04-09 00:00:00
abstract:MAIN CONCLUSION:The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated fro...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2373-6
更新日期:2015-12-01 00:00:00
abstract::Callus cells of Daucus carota L. have different phenylpropanoid pathways depending on the medium composition. Cells propagated on a medium with gibberellic acid do not accumulate cyanidin but incorporate [(14)C]phenylalanine into chlorogenic acid at a high rate. Cells grown on a medium free of gibberellic acid accumul...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384906
更新日期:1977-01-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii has been identified as a promising organism for the production of recombinant proteins. While during the last years important improvements have been developed for the production of proteins within the chloroplast, the expression levels of transgenes from the nuclear...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0879-x
更新日期:2009-03-01 00:00:00
abstract::2-(Chloroethyl)phosphonic acid (CEPA) inhibited the rise in tannin production and phenylalanine ammonia-lyase EC 4.1.1.5 (PAL) activity shown by Acer cells in media containing 9.0×10(-7) M 2,4-D for a period of 2-3 days after its addtion. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385416
更新日期:1976-01-01 00:00:00
abstract::A crude organelle preparation from germinating castor bean endosperm catalysed the incorporation of galactose from UDP[(14)C]galactose into chloroform/methanol (2:1)-soluble glactolipids. At least two galactolipids were formed. Most of the [(14)C]galactose was present in a galactolipid synthesized by the microsomal me...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384596
更新日期:1979-10-01 00:00:00
abstract::Vacuoles were isolated by osmotic rupture of mesophyll protoplasts from the primary leaves of 4-d- and 7-d-old plants of rye (Secale cereale L.). Their content of two flavones, luteolin 7-O-[β-D-glucuronosyl-(1→2)β-D-glucuronide] (R2) and luteolin 7-O-[β-D-glucuronosy 1 (1→2) β-D-glucuronide]-4'-O-β-D-glucuronide (R1)...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201627
更新日期:1992-04-01 00:00:00
abstract::We have screened a cDNA library prepared from tomato (Lycopersicon esculentum Mill. cv. VFNT Cherry) shoot tips for homeobox-containing clones. The isolated cDNA clone THOM1 (tomato homeobox gene 1) contains a homeobox, a leucine zipper motif and and an acidic region. These features support the hypothesis that the THO...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195713
更新日期:1995-01-01 00:00:00
abstract::The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [(14)C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained on the column. The...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390085
更新日期:1977-01-01 00:00:00
abstract::14C-IAA was applied to decapitated Coleus 5th internodes. Sections were radioautographed at various intervals after the application. The location of the label within cells and tissues is discussed. No accumulation of label occurred in the region where xylem cells regenerated. Label was particularly associated with xyl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386501
更新日期:1972-03-01 00:00:00
abstract::When plant storage tissue is cut into thin disks it rapidly develops a high capacity to absorb phosphate from dilute solutions. The recent trend has been to attribute the development of this ability to the increased metabolic activity of bacteria known to be containing the tissue. Data presented in this paper shows th...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387651
更新日期:1970-03-01 00:00:00
abstract::Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0471-1
更新日期:2007-06-01 00:00:00
abstract::Increasing blue light intensity inhibits the growth of Chlorella pyrenoidosa in glucose culture in which photosynthesis is blocked by DCMU, whereas red light supports growth which is the same as or better than that in dark controls.The action spectrum of light induced protein synthesis from exogenous glucose (photosyn...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384880
更新日期:1966-09-01 00:00:00
abstract::In etiolated rye seedlings (Secale cereale L.) the cytokinin supply controls leaf growth. Dry weight and total amino-nitrogen, protein, total nucleic acid, DNA, and rRNA levels were similarly lowered in leaves depleted of their endogenous cytokinin supply by early excision of the seedling roots and increased by 70-100...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385122
更新日期:1978-01-01 00:00:00
abstract::Freeze-fracturing of Funaria hygrometrica caulonema cells leads to a cleavage within the plasma membrane. The extraplasmatic and the plasmatic fracture faces differ in their particle density. The plasmatic fracture face in caulonema tip cells or in tip cells of side branches, but never in other caulonema cells, is fur...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00429759
更新日期:1984-04-01 00:00:00
abstract::Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394488
更新日期:1988-01-01 00:00:00
abstract::Metabolite levels and carbohydrates were investigated in the leaves of tobacco (Nicotiana tabacum L.) and leaves and tubers of potato (Solanum tuberosum L.) plants which had been transformed with pyrophosphatase from Escherichia coli. In tobacco the leaves contained two- to threefold less pyrophosphate than controls a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00216819
更新日期:1992-09-01 00:00:00
abstract::Sequestered particles of phytochrome (SAPs) were partially purified from red-light-irradiated oat coleoptiles. Phytochrome pelletability was enhanced by using buffers containing 10 mM Mg(2+) or high concentrations (0.6-0.8 M) of orthophosphate (Pi). Combining the pelletability of phytochrome in the presence of Mg(2+) ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197798
更新日期:1991-01-01 00:00:00
abstract::The surface of plants is covered by cuticular wax, which contains a mixture of very long-chain fatty acid (VLCFA) derivatives. This wax surface provides a hydrophobic barrier which reduces non-stomatal water loss. One component of the cuticular wax is the alkyl esters, which typically contain a VLCFA esterified to an ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0489-z
更新日期:2007-07-01 00:00:00
abstract::Flax (Linum usitatissimum L.) phloem fibers elongate considerably during their development and intrude between existing cells. We questioned whether fiber elongation is caused by cell tip growth or intercalary growth. Cells with tip growth are characterized by having two specific zones of cytoplasm in the cell tip, on...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-1536-2
更新日期:2005-11-01 00:00:00
abstract::Monoclonal antibodies have been raised against haustorial complexes isolated from pea (Pisum sativum L.) leaves infected by the biotrophic powdery mildew fungus Erysiphe pisi D.C. Immuno-localisation studies, using isolated haustorial complexes and infected pea leaf material, have shown that one of the antibodies, des...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197739
更新日期:1991-02-01 00:00:00
abstract::Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00380870
更新日期:1980-07-01 00:00:00
abstract::The water potentials of frozen leaves of Afroalpine plants were measured psychrometrically in the field. Comparison of these potentials with the osmotic potentials of an expressed cellular sap and the water potentials of ice indicated almost ideal freezing behaviour and suggested equilibrium freezing. On the basis of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397450
更新日期:1984-09-01 00:00:00
abstract::Protoplasts from cultured cells of soybean (Glycine max L.) and from sweet clover (Melilotus officinalis L.) mesophyll cells were fused with polyethylene glycol and subsequently cultured for six days. The resulting fusion products as well as unfused protoplasts of each parental species regenerated cell walls and divid...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390842
更新日期:1976-01-01 00:00:00
abstract::1. The following acid hydrolases are partially sedimentable from cell-free extracts of corn seedlings: protease (pH-optimum 4,2), phosphatase (pH-optimum 5,0 and 6,5), unspecific esterase (pH-optimum 5,2), RNase (pH-optimum 6,5), arylsulphatase-C (pH-optimum 5,0) and α- and β-amylase (pH-optimum 7,0 and 5,0 respective...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388148
更新日期:1966-03-01 00:00:00
abstract::Yield is the most important and complex trait for genetic improvement in crops, and marker-assisted selection enhances the improvement efficiency. The USDA rice mini-core collection derived from over 18,000 accessions of global origins is an ideal panel for association mapping. We phenotyped 203 O. sativa accessions f...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1405-0
更新日期:2011-08-01 00:00:00
abstract::Ultraviolet (UV)-absorbing phenolic compounds that have been shown to be protective against the damaging: effects of UV-B radiation (Tevini et al., 1991, Photochem. Photobiol. 53, 329-333) were found in the leaf epidermis of tropical mangrove tree species. These UV-absorbing phenolic compounds and leaf succulence func...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00216808
更新日期:1992-09-01 00:00:00
abstract::Exudate was collected fromRicinus communis L. cotyledons after cutting the hypocotyl. It contained high levels of sucrose and potassium, a low level of calcium, and a pH of approx. 7.5. After application of [(14)C] sucrose to the cotyledons, radioactivity could be recovered from the exudate, indicating that the exudat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00959524
更新日期:1988-06-01 00:00:00
abstract::The changes in packed cell volume and in nitrate content and conductivity of the medium during the growth cycle of cell suspension cultures from Petroselinum hortense Hoffm., Glycine max Merr., and Haplopappus gracilis A. Grey in a chemically defined medium were compared. In all three cases sigmoidal curves obtained f...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388694
更新日期:1975-01-01 00:00:00