Abstract：
:Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness and mechanism of interaction of the defensins with Phytophthora spp. have not been clearly characterized in planta. In this study, we expressed the Dahlia merckii defensin, DmAMP1, in papaya (Carica papaya L.), a plant highly susceptible to a root, stem, and fruit rot disease caused by Phytophthora palmivora. Extracts of total leaf proteins from transformed plants inhibited growth of Phytophthora in vitro and discs cut from the leaves of transformed plants inhibited growth of Phytophthora in a bioassay. Results from our greenhouse inoculation experiments demonstrate that expressing the DmAMP1 gene in papaya plants increased resistance against P. palmivora and that this increased resistance was associated with reduced hyphae growth of P. palmivora at the infection sites. The inhibitory effects of DmAMP1 expression in papaya suggest this approach has good potential to impart transgenic resistance against Phytophthora in papaya.
journal_name
Plantajournal_title
Plantaauthors
Zhu YJ,Agbayani R,Moore PHdoi
10.1007/s00425-006-0471-1subject
Has Abstractpub_date
2007-06-01 00:00:00pages
87-97issue
1eissn
0032-0935issn
1432-2048journal_volume
226pub_type
杂志文章相关文献
PLANTA文献大全abstract：:In order to better understand the various pathways of sucrose and starch catabolism in the anther of lily (Lilium hybrida var. "Enchantment"), invertase (EC 3.2.1.26) and amylase (EC 3.2.1.1, EC 3.2.1.2) activities were measured separately in different fractions (anther wall, locular fluid and microspore/pollen) and c...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0443-5
更新日期：2007-05-01 00:00:00
abstract：:We used RNA gel-blot analysis, in-situ hybridization, and nuclear run-on transcription to examine the effects of exogenous abscisic acid (ABA) on the spatial distribution of mRNA for the lectin wheat-germ agglutinin (WGA) in developing wheat (Triticum aestivum L. cv. Marshall) embryos and seedlings. When analyzed by R...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF02411453
更新日期：1990-03-01 00:00:00
abstract：:Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00454448
更新日期：1979-01-01 00:00:00
abstract：MAIN CONCLUSION:This study confirms a high level of metabolic resistance to the herbicide chlorsulfuron, inherited by a single dominant gene in a sorghum genotype (GL-1). Chlorsulfuron, an acetolactate synthase (ALS)-inhibitor, effectively controls post-emergence grass and broadleaf weeds but is not registered for use ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-020-03563-3
更新日期：2021-01-23 00:00:00
abstract：:The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on alpha-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Tr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050326
更新日期：1998-07-01 00:00:00
abstract：MAIN CONCLUSION:In this genome-wide association study, we obtained novel insights into the genetic basis of the effect of herbivory or drought stress on the level of resistance against the fungus Botrytis cinerea. In nature, plants function in complex environments where they encounter different biotic and abiotic stres...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-3065-9
更新日期：2019-04-01 00:00:00
abstract：:Three non-allelic radial swelling mutants (rsw1, rsw2 and rsw3) of Arabidopsis thaliana L. Heynh. were shown to be specifically impaired in cellulose production. Fractionation methods that identify, characterise and quantify some of the major cell wall polysaccharides in small quantities of seedlings demonstrated that...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000301
更新日期：2000-08-01 00:00:00
abstract：:Furfural uptake was studied with regard to possible mechanisms of inducing germination in ascospores. Uptake was found to involve a large, weakly bound reversible component and a small tightly bound irreversible component. Localization experiments indicate that almost all of the furfural removed from the media is boun...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385758
更新日期：1970-12-01 00:00:00
abstract：MAIN CONCLUSION:A novel GA13-oxidase ofTripterygium wilfordii, TwGA13ox, is a 2-oxoglutarate-dependent dioxygenase. It specifically catalyzes the conversion of GA9to GA20, but not GA4to GA1. Gibberellins (GAs) play essential roles in plant growth and development. Previous characterization of GA20- and GA3-oxidases yiel...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-019-03240-0
更新日期：2019-11-01 00:00:00
abstract：:Root growth was analyzed in Allium cepa L. grown under steady-state conditions at 10, 15 and 25°C by measuring cell sizes along the meristem and mature zones at these three temperatures and in the elongation zone at 15°C. The absolute rate of growth, v(x), was determined as a function of the mean cell number of cells ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402962
更新日期：1986-06-01 00:00:00
abstract：:The metabolic, biochemical and molecular events occurring during tobacco (Nicotiana tabacum) leaf ageing are presented, with a particular emphasis on nitrogen metabolism. An integrated model describing the source/sink relationship existing between leaves of different developmental stages along the main plant axis is p...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000310
更新日期：2000-09-01 00:00:00
abstract：:The pattern of protein synthesis was compared in several organs of maize (Zea mays L.) under aerobic and anaerobic conditions. Protein synthesis was measured by [(35)S]methionine incorporation and analysis by two-dimensional native-SDS (sodium lauryl sulfate) polyacrylamide gel electrophoresis and fluorography. The ae...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385619
更新日期：1980-11-01 00:00:00
abstract：:We have investigated the regulation of sucrose storage in cell-suspension cultures of sugarcane. When grown in batch culture, sucrose accumulation commences after about 5 d, when the nitrogen supply is exhausted. Sucrose storage is also induced by decreasing the nitrogen supply to cells growing in a chemostat. The mea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00197564
更新日期：1991-12-01 00:00:00
abstract：:Cadmium contamination is a serious environmental problem for modern agriculture and human health. Salinity affects plant growth and development, and interactions between salt and cadmium have been reported. However, the molecular mechanisms of salinity-cadmium interactions are not fully understood. Here, we show that ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-1070-8
更新日期：2010-01-01 00:00:00
abstract：:Protoplasts of Vicia hajastana Grossh. obtained from suspension-culture cells and Pisum sativum L. obtained from leaves adhered tightly to each other in concentrated solutions of high-molecular-weight polyethylene glycol (PEG). The adhesion occurred non-specifically between the free protoplasts from the same species a...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388618
更新日期：1974-12-01 00:00:00
abstract：MAIN CONCLUSION:Phloem production and structural development were interlinked with seasonal variation in the primary and secondary metabolites of phloem. Novel microtechniques provided new perspectives on understanding phloem structure and chemistry. To gain new insights into phloem formation in Norway spruce (Picea ab...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-015-2347-8
更新日期：2015-09-01 00:00:00
abstract：:Cotton fibers are single elongated cells that develop from epidermal cells of the ovule. The chronology of fiber differentiation was investigated using cultured ovules. Epidermal cells differentiate into fiber cells approx. 3 d before anthesis. When ovules were cultured on a defined medium, fiber growth could be initi...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392435
更新日期：1988-08-01 00:00:00
abstract：:Previous studies have led to the identification and characterization of specific, high-affinity binding sites for a hepta-beta-glucoside elicitor in soybean. A survey of plant species for elicitor-binding activity reveals that among the plants tested, the hepta-beta-glucoside elicitor is only recognized by plants belo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000325
更新日期：2000-09-01 00:00:00
abstract：:The plastid terminal oxidase (PTOX) is a plastoquinol oxidase involved in carotenoid biosynthesis in higher plants, and may also represent the elusive oxidase in chlororespiration. Haematococcus pluvialis is a green alga that has the ability to synthesize and accumulate large amounts of the red carotenoid astaxanthin ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-0932-4
更新日期：2009-06-01 00:00:00
abstract：:The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and bind to the DRE/CRT cis-acting element (core motif: G/ACCGAC) commonly present in cold-regulated (COR) genes and subsequently upregulate the expression of such genes in Arabidopsis. We...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0273-5
更新日期：2006-09-01 00:00:00
abstract：MAIN CONCLUSION:Trehalose increased drought tolerance of tomato plants, accompanied by reduced water loss and closed stomata, which was associated with the upregulated ABA signaling-related genes expression, but not in ABA accumulation. Drought is one of the principal abiotic stresses that negatively influence the grow...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-019-03195-2
更新日期：2019-08-01 00:00:00
abstract：MAIN CONCLUSION:Different autophagy pathways are a driver of vacuolar biogenesis and are development stage specific during the extrafloral nectary development in Citharexylum myrianthum. Plant autophagy plays an important role in various developmental processes such as seed germination, pollen maturation and leaf senes...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-019-03190-7
更新日期：2019-08-01 00:00:00
abstract：MAIN CONCLUSION:ST1 and ST6 are possibly involved in primary and lateral root and symbiotic nodule development, but only ST6 participates in the interaction with hemibiotrophic fungi. Specific tissue (ST) proteins have been shown to be involved in several processes related to plant nutritional status, development, and ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-020-03538-4
更新日期：2021-01-02 00:00:00
abstract：:During sunflower (Helianthus annuus L.) seed formation there was an active period of lipid biosynthesis between 12 and 28 days after flowering (DAF). The maximum in-vitro acyl-acyl carrier protein (ACP) thioesterase activities (EC 3.1.2.14) were found at 15 DAF, preceding the largest accumulation of lipid in the seed....
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000332
更新日期：2000-10-01 00:00:00
abstract：:The polarity of germinating single-celled spores of the fern Ceratopteris richardii Brogn. is influenced by gravity during a time period prior to the first cellular division designated a "polarity-determination window". After this window closes, control of polarity is seen in the downward (with respect to gravity) mig...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050355
更新日期：1998-08-01 00:00:00
abstract：:A method is presented for mass isolation of metaphase chromosomes and nuclei from plant protoplasts. The isolation procedure was developed for both monocotyledonous and dicotyledonous species using wheat (Triticum monococcum) and poppy (Papaver somniferum) cell cultures. Metaphase chromosomes were isolated from partia...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00405195
更新日期：1983-04-01 00:00:00
abstract：:To test the morphogenetic role of equilateral stress and physiological confinement in dedifferentiation of epidermal cells, impermeable barriers of goldmetal foil were placed between the postgenitally fusing epidermal surfaces in the gynoecium of Catharanthus roseus (L.) G. Don. Cells contacting the foil barrier were ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388210
更新日期：1978-01-01 00:00:00
abstract：:The permeability of biological membranes to boric acid was investigated using the giant internodal cells of the charophyte alga Chara corallina (Klein ex Will. Esk. R.D. Wood). The advantage of this system is that it is possible to distinguish between membrane transport of boron (B) and complexing of B by plant cell w...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000484
更新日期：2001-05-01 00:00:00
abstract：:No phytochrome can be detected spectrophotometrically in soybean (Glycine max (L.) Merr. cv. Mandarin) cell suspensions grown in darkness. White light from a tungsten filament lamp and a band of far-red light have been found to induce the appearance of phytochrome in them. Red light failed to bring about phytochrome i...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390095
更新日期：1977-01-01 00:00:00
abstract：:The free amino acid pools in the nitrogen-fixing blue-green algae Anabaena cylindrica, A. flos-aquae and Westiellopsis prolifica contain a variety of amino acids with aspartic acid, glutamic acid and the amide glutamine being present in much higher concentrations than the others. This pattern is characteristic of that...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386075
更新日期：1972-06-01 00:00:00
