Effects of (+)-8',8',8'-trifluoroabscisic acid on alpha-amylase expression and sugar accumulation in rice cells.


:The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on alpha-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Treatment with (+)-trifluoro-ABA strongly inhibited the gibberellic acid-inducible expression of alpha-amylase I-1 encoded by RAmy1A in the aleurone layers of embryoless half-seeds at the levels of transcription, protein synthesis, and enzyme activity. It was also found that (+)-trifluoro-ABA stimulated (i) the uptake of glucose from the incubation medium and (ii) the synthesis of sucrose in scutellar tissues and suspension-cultured cells of rice. The biological activity of (+)-trifluoro-ABA was found to be more potent and persistent than that of natural ABA. We further examined the effects of trifluoro-ABA on the expression of alpha-amylase I-1 in scutellar tissues and suspension-cultured cells. It was found that (+)-trifluoro-ABA did not inhibit the formation of alpha-amylase I-1 in the absence of external glucose. However, glucose and (+)-trifluoro-ABA cooperatively suppressed the formation of alpha-amylase I-1. Judging from these results, we conclude that the regulatory mechanism for the expression of alpha-amylase I-1 in the scutellar epithelium is distinguishable from that operating in the aleurone layer.






Kashem MA,Hori H,Itoh K,Hayakawa T,Todoroki Y,Hirai N,Ohigashi H,Mitsui T




Has Abstract


1998-07-01 00:00:00














  • Functional testing of a PF02458 homologue of putative rice arabinoxylan feruloyl transferase genes in Brachypodium distachyon.

    abstract:MAIN CONCLUSION:We show that changing the expression of a putative feruloyl transferase gene belonging to the BAHD acyl-transferase family alters the levels of cell wall esterified ferulates and diferulates in Brachypodium distachyon cell walls. While the potential of grass cell walls for biofuel production has been re...


    pub_type: 杂志文章


    authors: Buanafina MM,Fescemyer HW,Sharma M,Shearer EA

    更新日期:2016-03-01 00:00:00

  • The immuno-histochemical localization of lectin in pea seeds (Pisum sativum L.).

    abstract::The lectin from the garden pea (Pisum sativum L.) has been localized at the ultrastructural level by the unlabeled peroxidase-antiperoxidase procedure of L.A. Sternberger et al. (1970, J. Histochem. Cytochem 18, 315-333) in 24 h imbibed seeds. Upon examination by light microscopy and transmission electron microscopy, ...


    pub_type: 杂志文章


    authors: Van Driessche E,Smets G,Dejaegere R,Kanarek L

    更新日期:1981-12-01 00:00:00

  • The role of the apex in the phototropic curvature of Avena coleoptiles: positive curvature under conditions of continuous illumination.

    abstract::The differential growth causing second positive phototropic curvature in intact, black-capped and decapitated Avena coleoptiles has been measured. In all cases the curvature is brought about by a cessation in growth of the illuminated side. The fact that shading the apex does not significantly alter the initial steps ...


    pub_type: 杂志文章


    authors: Franssen JM,Firn RD,Digby J

    更新日期:1982-08-01 00:00:00

  • A specific bioassay for the inhibition of flowering.

    abstract::A bioassay for the inhibition of flowering involving the in vitro culture of excised, partially-induced, apices of Viscaria candida is described. This bioassay has been used to detect flowering inhibition in extracts from Kalanchoe blossfeldiana. ...


    pub_type: 杂志文章


    authors: Blake J

    更新日期:1972-06-01 00:00:00

  • Xyloglucan endo-transglycosylase/hydrolase (XET/H) gene is expressed during the seed germination in Podophyllum hexandrum: a high altitude Himalayan plant.

    abstract:MAIN CONCLUSION:Xyloglucan endo-transglycosylase/hydrolase ( Ph XET/H) regulates Podophyllum seed germination via GA mediated up-accumulation of Ph XET protein and subsequent endosperm weakening. Xyloglucan endo-transglycosylase/hydrolase (XET/H) belong to glycosyl hydrolase family 16, which play an important role in e...


    pub_type: 杂志文章


    authors: Dogra V,Sharma R,Yelam S

    更新日期:2016-08-01 00:00:00

  • A Ser/Thr protein kinase phosphorylates MA-ACS1 (Musa acuminata 1-aminocyclopropane-1-carboxylic acid synthase 1) during banana fruit ripening.

    abstract::1-Aminocyclopropane-1-carboxylic acid synthase (ACS) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. ACS isozymes are regulated both transcriptionally and post-translationally. However, in banana, an important climacteric fruit, little is known about post-translational regulation of ACS. Her...


    pub_type: 杂志文章


    authors: Choudhury SR,Roy S,Sengupta DN

    更新日期:2012-08-01 00:00:00

  • Expression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection.

    abstract::Callose synthesis occurs at specific stages of plant cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We determined the expression pattern of "upstream regulatory sequence" of 12 Arabidopsis callose synthase genes (CalS1-12) genes and demonstrated that d...


    pub_type: 杂志文章


    authors: Dong X,Hong Z,Chatterjee J,Kim S,Verma DP

    更新日期:2008-12-01 00:00:00

  • Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.

    abstract::The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots...


    pub_type: 杂志文章


    authors: Otto B,Kaldenhoff R

    更新日期:2000-07-01 00:00:00

  • Glutamine synthetase in the chloroplasts of Vicia faba.

    abstract::A glutamine synthetase has been localised in the chloroplasts of Vicia faba. The enzyme has requirements for Mg(2+) and ATP in the biosynthetic reaction and in addition will catalyse a γ-glutamyl transferase reaction in the presence of Mn(2+) and arsenate. The enzyme is inhibited by AMP, CTP, glycine and alanine. Thes...


    pub_type: 杂志文章


    authors: Haystead A

    更新日期:1973-09-01 00:00:00

  • The novel gene CpEdi-9 from the resurrection plant C. plantagineum encodes a hydrophilic protein and is expressed in mature seeds as well as in response to dehydration in leaf phloem tissues.

    abstract::The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem...


    pub_type: 杂志文章


    authors: Rodrigo MJ,Bockel C,Blervacq AS,Bartels D

    更新日期:2004-08-01 00:00:00

  • The nutritional status of the apical meristem of Lactuca sativa as affected by NaCl salinization: An electron-probe microanalytic study.

    abstract::A volume of tissue of lettuce (Lactuca sativa L.) plants extending 2 mm basipetally from the apical meristem and including leaf primordia and young expanding leaves was surveyed using electron-probe microanalysis (EPMA) on both frozen-hydrated and freeze-dried samples. This analysis was carried out either 2 or 5 d fol...


    pub_type: 杂志文章


    authors: Lazof D,Läuchli A

    更新日期:1991-06-01 00:00:00

  • Diffusible gibberellins and phototropism in Helianthus annuus.

    abstract::Endogenous gibberellins were obtained in agar from the lower cut surface of upright sunflower shoot-tips. Exposure to unilateral light of the tips standing on agar, with the lower cut ends bisected by a vertical glass barrier at right angles to incident light, resulted in approximately 8 times the quantity of gibberel...


    pub_type: 杂志文章


    authors: Phillips ID

    更新日期:1972-12-01 00:00:00

  • [Metabolism of γ-aminobutyrate in Agaricus bisporus Lge. : II: γ-aminobutyrate: γ-ketoglutarate aminotransferase].

    abstract::Transamination between γ-aminobutyrate and α-ketoglutarate provides a pathway for the utilization of γ-aminobutyrate in fruit-bodies of Agaricus bisporus Lge. This reaction leads to the formation of succinic semialdehyde, a metabolic intermediate in the metabolism of γ-aminobutyrate to succinate in the cell. γ-aminobu...


    pub_type: 杂志文章


    authors: Baldy P

    更新日期:1976-01-01 00:00:00

  • Chemical and immunological similarities between the phloem proteins of three genera of the Cucurbitaceae.

    abstract::Phloem exudates from Cucurbita, Cucumis, and Citrullus were gelled by oxidative formation of disulphide bridges between the phloem filaments. Gellation could be inhibited by dithiothreitol or iodoacetamide and did not require the presence of the phloem lectin. Each exudate contained a dimeric lectin of similar relativ...


    pub_type: 杂志文章


    authors: Read SM,Northcote DH

    更新日期:1983-06-01 00:00:00

  • Photoprotection of green plants: a mechanism of ultra-fast thermal energy dissipation in desiccated lichens.

    abstract::In order to survive sunlight in the absence of water, desiccation-tolerant green plants need to be protected against photooxidation. During drying of the chlorolichen Cladonia rangiformis and the cyanolichen Peltigera neckeri, chlorophyll fluorescence decreased and stable light-dependent charge separation in reaction ...


    pub_type: 杂志文章


    authors: Heber U

    更新日期:2008-09-01 00:00:00

  • Lateral movement out of the sieve tubes and its effect on the (14)C translocation profile in Helianthus seedlings.

    abstract::The profile of (14)C, that is the plot of log (radioactivity) against distance, down the stem of Helianthus seedlings was studied over the course of the first hour after feeding (14)C to a leaf. These seedlings are shown to accumulate a large proportion of the total (14)C present in the stem in an ethanol insoluble fo...


    pub_type: 杂志文章


    authors: Whittle CM

    更新日期:1970-09-01 00:00:00

  • Soluble and microsomal glutatione S-transferase activities in pea seedlings (Pisum sativum L.).

    abstract::Epicotyl and primary leaves of pea seedlings (Pisum sativum L., var. Alaska) were found to contain soluble and microsomal enzymes catalyzing the addition of glutathione to the olefinic double bond of cinnamic acid. Glutathione S-cinnamoyl transfer was also obtained with enzyme preparations from potato slices and cell ...


    pub_type: 杂志文章


    authors: Diesperger H,Sandermann H Jr

    更新日期:1979-10-01 00:00:00

  • Decarboxylation and transport of auxin in segments of sunflower and cabbage roots.

    abstract::The movement of (14)C from indole-3-acetic acid (IAA) (14)C has been examined in 5 mm root segments of dark-grown seedlings of Helianthus annuus and Brassica oleracea. Contaminants from distilled water, phosphate buffer and the razor-blade cutter increase the decarboxylation of IAA-(14)C, and cutting of root segments ...


    pub_type: 杂志文章


    authors: Iversen TH,Aasheim T

    更新日期:1970-12-01 00:00:00

  • The ethylene receptor regulates Typha angustifolia leaf aerenchyma morphogenesis and cell fate.

    abstract:MAIN CONCLUSION:Ethylene receptor is crucial for PCD and aerenchyma formation in Typha angustifolia leaves. Not only does it receive and deliver the ethylene signal, but it probably can determine the cell fate during aerenchyma morphogenesis, which is due to the receptor expression quantity. Aquatic plant oxygen delive...


    pub_type: 杂志文章


    authors: Liu H,Hao N,Jia Y,Liu X,Ni X,Wang M,Liu W

    更新日期:2019-07-01 00:00:00

  • A single gene inherited trait confers metabolic resistance to chlorsulfuron in grain sorghum (Sorghum bicolor).

    abstract:MAIN CONCLUSION:This study confirms a high level of metabolic resistance to the herbicide chlorsulfuron, inherited by a single dominant gene in a sorghum genotype (GL-1). Chlorsulfuron, an acetolactate synthase (ALS)-inhibitor, effectively controls post-emergence grass and broadleaf weeds but is not registered for use ...


    pub_type: 杂志文章


    authors: Pandian BA,Sathishraj R,Prasad PVV,Jugulam M

    更新日期:2021-01-23 00:00:00

  • Control of ion absorption by phytochrome.

    abstract::Phosphate absorption by segments excised from hypocotyls of Phaseolus aureus Roxb. was inhibited by either continuous or short-term illumination with red light. Maximal inhibition was observed at pH 6.5. Illumination with 800 erg cm(-2) s(-1) of red light for 5 min was sufficient to obtain maximal inhibition. The effe...


    pub_type: 杂志文章


    authors: Tezuka T,Yamamoto Y

    更新日期:1975-01-01 00:00:00

  • Porphyra nereocystis: A dual-daylength seaweed.

    abstract::Conchospores from the perennial conchocelis phase of the annual, epiphytic, marine red alga Porphyra nereocystis Anderson, which in nature lives on the large annual kelp Nereocystis luetkeana (Mertens) Postels et Ruprecht, are released in culture only in response to a short-day photoperiod treatment followed by a long...


    pub_type: 杂志文章


    authors: Dickson LG,Waaland JR

    更新日期:1985-09-01 00:00:00

  • Regulation of stipule development by COCHLEATA and STIPULE-REDUCED genes in pea Pisum sativum.

    abstract::Pisum sativum L., the garden pea crop plant, is serving as the unique model for genetic analyses of morphogenetic development of stipule, the lateral organ formed on either side of the junction of leafblade petiole and stem at nodes. The stipule reduced (st) and cochleata (coch) stipule mutations and afila (af), tendr...


    pub_type: 杂志文章


    authors: Kumar S,Mishra RK,Kumar A,Srivastava S,Chaudhary S

    更新日期:2009-08-01 00:00:00

  • Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion.

    abstract:MAIN CONCLUSION:A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion. Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most ...


    pub_type: 杂志文章


    authors: Pierce ET,Graham BP,Stiff MR,Osborne JA,Haigler CH

    更新日期:2019-05-01 00:00:00

  • The life of phi: the development of phi thickenings in roots of the orchids of the genus Miltoniopsis.

    abstract::Phi thickenings, bands of secondary wall thickenings that reinforce the primary wall of root cortical cells in a wide range of species, are described for the first time in the epiphytic orchid Miltoniopsis. As with phi thickenings found in other plants, the phi thickenings in Miltoniopsis contain highly aligned cellul...


    pub_type: 杂志文章


    authors: Idris NA,Collings DA

    更新日期:2015-02-01 00:00:00

  • Two forms of NADP-dependent malic enzyme in expanding maize leaves.

    abstract::Etiolated maize leaves (Zea mays L.) contain a major isozyme of NADP-dependent malic enzyme (L-malate dehydrogenase, decarboxylating, EC having an isoelectric point of 5.28±0.03, a Km (L-malate) 0.3-0.6 mM at pH 7.45; a broad pH optimum around pH 6.9 under the conditions of assay; a molecular weight of 280,0...


    pub_type: 杂志文章


    authors: Pupillo P,Bossi P

    更新日期:1979-01-01 00:00:00

  • The effects of temperature and IAA concentration on the latent period for IAA-induced rapid growth of Avena coleoptile segments.

    abstract::Indoleacetic acid buffered at pH 7.0 induces a high growth rate in Avena coleoptile segments after a latent period, the duration of which is dependent upon both IAA concentration and temperature. A minimum latent period of 7.3 min is observed at 25° C with 10(-3) M IAA in phosphate buffer at pH 7.0.In contrast, 5×10(-...


    pub_type: 杂志文章


    authors: Philipson JJ,Hillman JR,Wilkins MB

    更新日期:1973-12-01 00:00:00

  • Floral visitation and reproductive traits of Stamenoid petals, a naturally occurring floral homeotic variant of Capsella bursa-pastoris (Brassicaceae).

    abstract::Homeotic changes played a considerable role during the evolution of flowers, but how floral homeotic mutants initially survive in nature has remained enigmatic. To better understand the evolutionary potential of floral homeotic mutants, we established as a model system Stamenoid petals (Spe), a natural variant of Caps...


    pub_type: 杂志文章


    authors: Ziermann J,Ritz MS,Hameister S,Abel C,Hoffmann MH,Neuffer B,Theissen G

    更新日期:2009-11-01 00:00:00

  • Nitrogenase activity, amino acid pool patterns and amination in blue-green algae.

    abstract::The free amino acid pools in the nitrogen-fixing blue-green algae Anabaena cylindrica, A. flos-aquae and Westiellopsis prolifica contain a variety of amino acids with aspartic acid, glutamic acid and the amide glutamine being present in much higher concentrations than the others. This pattern is characteristic of that...


    pub_type: 杂志文章


    authors: Dharmawardene MW,Stewart WD,Stanley SO

    更新日期:1972-06-01 00:00:00

  • Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor.

    abstract::Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced anne...


    pub_type: 杂志文章


    authors: Chu P,Chen H,Zhou Y,Li Y,Ding Y,Jiang L,Tsang EW,Wu K,Huang S

    更新日期:2012-06-01 00:00:00