Nitrogenase activity, amino acid pool patterns and amination in blue-green algae.

Abstract:

:The free amino acid pools in the nitrogen-fixing blue-green algae Anabaena cylindrica, A. flos-aquae and Westiellopsis prolifica contain a variety of amino acids with aspartic acid, glutamic acid and the amide glutamine being present in much higher concentrations than the others. This pattern is characteristic of that found in organisms having glutamine synthetage/glutamate synthetase [glutamine amide-2-oxoglutarate amino transferase (oxido-reductase)] as an important pathway of ammonia incorporation. Under nitrogen-starved conditions the level of acetylene reduction (nitrogen fixation) and the glutamine pool both increase but the free ammonia pool decreases, suggesting that ammonia rather than glutamine regulates nitrogen fixation.Glutamine synthetase has been demonstrated in Anabaena cylindrica using the γ-glutamyl transferase assay and also using a biosynthetic assay in which Pi release from ATP during glutamine synthesis was measured. The enzyme (γ-glutamyl transferase assay) is present in nitrogen-fixing cultures and activity is higher in aerobic than in microaerophilic cultures. Ammonium-grown cultures have lowest levels of all and activity in the presence of nitrate-nitrogen (150 mg nitrogen 1(-1)) is lower than in aerobic cultures growing on elemental nitrogen. Ammonium-nitrogen and nitrate-nitrogen have no effect on glutamine synthetase in vitro. Glutamate synthetase also operates in nitrogen-fixing cultures of Anabaena cylindrica.

journal_name

Planta

journal_title

Planta

authors

Dharmawardene MW,Stewart WD,Stanley SO

doi

10.1007/BF00386075

subject

Has Abstract

pub_date

1972-06-01 00:00:00

pages

133-45

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

108

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Onset of desiccation tolerance during development of the barley embryo.

    abstract::We have investigated events which take place in the developing barley (Hordeum vulgare L.) embryo during its acquisition of desiccation tolerance. Excised embryos are capable of precocious germination as early as 8 d after pollination (DAP). At this age, however, they are not capable of resisting a desiccation treatme...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00393069

    authors: Bartels D,Singh M,Salamini F

    更新日期:1988-10-01 00:00:00

  • Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries.

    abstract::Water deficits consistently promote higher concentrations of anthocyanins in red winegrapes and their wines. However, controversy remains as to whether there is any direct effect on berry metabolism other than inhibition of growth. Early (ED) and late (LD) season water deficits, applied before or after the onset of ri...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-007-0598-8

    authors: Castellarin SD,Matthews MA,Di Gaspero G,Gambetta GA

    更新日期:2007-12-01 00:00:00

  • Increased expression of a plant actin gene during a biotrophic interaction between round-leaved mallow, Malva pusilla, and Colletotrichum gloeosporioides f. sp. malvae.

    abstract::Two actin genes, actA from the hemibiotrophic anthracnose fungus, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. f. sp. malvae, and act1 from its host, Malva pusilla (Sm.) were cloned from a cDNA library developed from infected host tissue. The actin gene, actA, of C. gloeosporioides f. sp. malvae, which is simi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050752

    authors: Jin S,Xu R,Wei Y,Goodwin PH

    更新日期:1999-10-01 00:00:00

  • A proximal upstream sequence controls tissue-specific expression of Lem2, a salicylate-inducible barley lectin-like gene.

    abstract::The lemma and palea (lemma/palea), which form the husk of barley (Hordeum vulgare L.) seeds, constitutively express high levels of defense-related genes, relative to leaves [Abebe et al. (2004) Crop Sci 44:942-950]. One of these genes, Lem2, is expressed mainly in the lemma/palea and coleoptile and is strongly upregul...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1429-9

    authors: Abebe T,Skadsen RW,Kaeppler HF

    更新日期:2005-05-01 00:00:00

  • The light-induced reduction of the gravitropic growth-orientation of seedlings of Arabidopsis thaliana (L.) Heynh. is a photomorphogenic response mediated synergistically by the far-red-absorbing forms of phytochromes A and B.

    abstract::Hypocotyls of dark-grown seedlings of Arabidosis thaliana exhibit a strong negative gravitropism, which is reduced by red and also by long-wavelength, far-red light treatments. Light treatments using phytochrome A (phyA)- and phytochrome B (phyB)-deficient mutants showed that this response is controlled by phyB in a r...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00195180

    authors: Poppe C,Hangarter RP,Sharrock RA,Nagy F,Schäfer E

    更新日期:1996-01-01 00:00:00

  • Comparative transcriptomic analysis between 'Summer Black' and its bud sport 'Nantaihutezao' during developmental stages.

    abstract:MAIN CONCLUSION:4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played essential roles in contributing to the total soluble solid and total anthocyanin variations induced by bud sport in grape berries. Taste and color, which are important organoleptic qualities of grape berry, un...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03543-7

    authors: Leng F,Ye Y,Zhu X,Zhang Y,Zhang Z,Shi J,Shen N,Jia H,Wang L

    更新日期:2021-01-05 00:00:00

  • Overexpression of wheat transcription factor (TaHsfA6b) provides thermotolerance in barley.

    abstract:MAIN CONCLUSION:Overexpressing a heat shock factor gene (TaHsfA6bT) from wheat provides thermotolerance in barley by constitutive expression of heat and other abiotic stress-response genes. Temperature is one of the most crucial abiotic factors defining the yield potential of temperate cereal crops, such as barley. The...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03457-4

    authors: Poonia AK,Mishra SK,Sirohi P,Chaudhary R,Kanwar M,Germain H,Chauhan H

    更新日期:2020-09-18 00:00:00

  • Gas chromatographic-mass spectroscopic identification and quantification of arachidonic acid in wheat-germ oil.

    abstract::Gas chromatographic-mass spectroscopic evidence is presented for the presence of arachidonic acid ((all-Z)-5,8,11,14-eicosatetraenoic acid) in virgin wheat-germ oil. The amount of arachidonic acid in wheat-germ oil, determined as methyl ester using gas chromatographic detection, was quantified with reference to a stan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00429462

    authors: Janistyn B

    更新日期:1982-08-01 00:00:00

  • Analysis of the phytochrome gene family in Ceratodon purpureus by gene targeting reveals the primary phytochrome responsible for photo- and polarotropism.

    abstract::Using gene targeting by homologous recombination in Ceratodon purpureus, we were able to knock out four phytochrome photoreceptor genes independently and to analyze their function with respect to red light dependent phototropism, polarotropism, and chlorophyll content. The strongest phenotype was found in knock-out li...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0922-6

    authors: Mittmann F,Dienstbach S,Weisert A,Forreiter C

    更新日期:2009-06-01 00:00:00

  • Systematic identification and characterization of circular RNAs involved in flag leaf senescence of rice.

    abstract:MAIN CONCLUSION:Circular RNAs (circRNAs) identification, expression profiles, and construction of circRNA-parental gene relationships and circRNA-miRNA-mRNA ceRNA networks indicate that circRNAs are involved in flag leaf senescence of rice. Circular RNAs (circRNAs) are a class of 3'-5' head-to-tail covalently closed no...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03544-6

    authors: Huang X,Zhang H,Guo R,Wang Q,Liu X,Kuang W,Song H,Liao J,Huang Y,Wang Z

    更新日期:2021-01-07 00:00:00

  • Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species.

    abstract::The expression of a gene, encoding a dehydrin protein designated as DHN24 was analyzed at the protein level in two groups of Solanum species differing in cold acclimation ability. The DHN24 protein displays consensus amino acid sequences of dehydrins, termed K- and S-segments. The S-segment precedes three K-segments, ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0200-1

    authors: Rorat T,Szabala BM,Grygorowicz WJ,Wojtowicz B,Yin Z,Rey P

    更新日期:2006-06-01 00:00:00

  • Bambara groundnut: an exemplar underutilised legume for resilience under climate change.

    abstract:MAIN CONCLUSION:Bambara groundnut has the potential to be used to contribute more the climate change ready agriculture. The requirement for nitrogen fixing, stress tolerant legumes is clear, particularly in low input agriculture. However, ensuring that existing negative traits are tackled and demand is stimulated throu...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-019-03191-6

    authors: Mayes S,Ho WK,Chai HH,Gao X,Kundy AC,Mateva KI,Zahrulakmal M,Hahiree MKIM,Kendabie P,Licea LCS,Massawe F,Mabhaudhi T,Modi AT,Berchie JN,Amoah S,Faloye B,Abberton M,Olaniyi O,Azam-Ali SN

    更新日期:2019-09-01 00:00:00

  • The localization of enzymes in the cotyledons of Pisum arvense L. during germination.

    abstract::Enzyme activity is not uniformly distributed through the cotyledon of Pisum arvense. Initially the peripheral region, certain scattered cells of the storage tissue and the procambium show a high level of activity of succinic dehydrogenase, cytochrome oxidase, acid phosphatase and esterase. Activity of acid phosphatase...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00380834

    authors: Flinn AM,Smith DL

    更新日期:1967-03-01 00:00:00

  • Effect of 2,4-dichlorophenoxyacetic acid on the structure and function of developing chloroplasts.

    abstract::Dark-grown radish seedlings (Raphanus sativus L.) were sprayed with 10(-3) mol·l(-1) 2,4-dichlorophenoxyacetic acid and then were exposed to a 14:10 light: dark cycle. Cotyledon samples from these seedlings and unsprayed controls were taken for electron microscopy, chlorophyll determinations, and photosynthetic rate m...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394437

    authors: Nadakavukaren MJ,McCracken DA

    更新日期:1977-01-01 00:00:00

  • Occurrence and environmental stress responses of two plastid terminal oxidases in Haematococcus pluvialis (Chlorophyceae).

    abstract::The plastid terminal oxidase (PTOX) is a plastoquinol oxidase involved in carotenoid biosynthesis in higher plants, and may also represent the elusive oxidase in chlororespiration. Haematococcus pluvialis is a green alga that has the ability to synthesize and accumulate large amounts of the red carotenoid astaxanthin ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0932-4

    authors: Wang J,Sommerfeld M,Hu Q

    更新日期:2009-06-01 00:00:00

  • A novel embryo-specific barley cDNA clone encodes a protein with homologies to bacterial glucose and ribitol dehydrogenase.

    abstract::In order to analyze the genetic programme expressed during the early stages of embryogenesis a cDNA clone bank was constructed from desiccation-tolerant excised barley embryos 18 d after pollination (D. Bartels et al., 1988, Planta 175, 485-492). One of the selected cDNA clones pG31 encodes a transcript of 1300 nucleo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00203590

    authors: Alexander R,Alamillo JM,Salamini F,Bartels D

    更新日期:1994-01-01 00:00:00

  • Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis.

    abstract::Raffinose (O-alpha- D-galactopyranosyl-(1-->6)- O-alpha- D-glucopyranosyl-(1<-->2)- O-beta- D-fructofuranoside) is a widespread oligosaccharide in plant seeds and other tissues. Raffinose synthase (EC 2.4.1.82) is the key enzyme that channels sucrose into the raffinose oligosaccharide pathway. We here report on the is...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0804-7

    authors: Peterbauer T,Mach L,Mucha J,Richter A

    更新日期:2002-09-01 00:00:00

  • The effect of the endosperm on the formation of gibberellin by barley embryos.

    abstract::It has been shown that the scuttellum of germinating barley embryos synthesises gibberellin in intact grains or when detached from the endosperm, but not when attached to the endosperm if the axis is removed. Evidence is given to support the hypothesis that the inhibition of gibberellin synthesis is caused by a distur...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386454

    authors: Radley M

    更新日期:1969-09-01 00:00:00

  • Equilibrium freezing of leaf water and extracellular ice formation in Afroalpine 'giant rosette' plants.

    abstract::The water potentials of frozen leaves of Afroalpine plants were measured psychrometrically in the field. Comparison of these potentials with the osmotic potentials of an expressed cellular sap and the water potentials of ice indicated almost ideal freezing behaviour and suggested equilibrium freezing. On the basis of ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397450

    authors: Beck E,Schulze ED,Senser M,Scheibe R

    更新日期:1984-09-01 00:00:00

  • Transcription and translation for carotenoid synthesis in Chlamydomonas reinhardtii.

    abstract::The sites of transcription and translation of carotenoid pigments were studied in synchronously grown cells of Chlamydomonas reinhardtii Dang. Rifampicin, cycloheximide and spectinomycin were used to distinguish between the nuclear-cytoplasmic genetic system and the genetic system of the chloroplast. Since rifampicin ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386737

    authors: Sirevåg R,Levine RP

    更新日期:1973-03-01 00:00:00

  • Origin and developmental changes of envelope proteins and translocator activities from plastids of Secale cereale L.

    abstract::To determine the sites of synthesis of chloroplast-envelope proteins, we have analysed several enzyme and translocator functions ascribed to the envelope membranes, and investigated the envelope polypeptide composition of plastids isolated from 70S ribosome-deficient leaves of rye (Secale cereale L.) generated by grow...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391269

    authors: Höinghaus R,Feierabend J

    更新日期:1985-12-01 00:00:00

  • Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

    abstract:MAIN CONCLUSION:Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spre...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-018-03084-0

    authors: Lambein F,Travella S,Kuo YH,Van Montagu M,Heijde M

    更新日期:2019-09-01 00:00:00

  • Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs.

    abstract::Plant defence strategies usually involve the action of several gene products. Transgenic resistance strategies are likely to have enhanced efficacy when they involve more than one transgene. Here we explore possible mechanisms for the co-delivery of multiple effectors via a single transgene. As an example we report th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050281

    authors: Urwin PE,McPherson MJ,Atkinson HJ

    更新日期:1998-04-01 00:00:00

  • Polyamines are essential for the synthesis of 2-ricinoleoyl phosphatidic acid in developing seeds of castor.

    abstract::The major fatty acid component of castor (Ricinus communis L.) oil is ricinoleic acid (12-hydroxy-cis-9-octadecenoic acid), and unsaturated hydroxy acid accounts for >85% of the total fatty acids in triacylglycerol (TAG). TAG had a higher ricinoleate content at position 2 than at positions 1 and 3. Although lysophosph...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0083-1

    authors: Tomosugi M,Ichihara K,Saito K

    更新日期:2006-01-01 00:00:00

  • Developmental, cytological and transcriptional analysis of autotetraploid Arabidopsis.

    abstract::An autopolyploid that contains more than two sets of the same chromosomes causes apparent alterations in morphology, development, physiology and gene expression compared to diploid. However, the mechanisms for these changes remain largely unknown. In the present study, cytological observations of mature embryos and gr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1629-7

    authors: Li X,Yu E,Fan C,Zhang C,Fu T,Zhou Y

    更新日期:2012-08-01 00:00:00

  • Physiological aspects of conjugation in fission yeast.

    abstract::Conjugation was studied in Schizosaccharomyces pombe using liquid media. Nitrogen, which was growth-limiting in a synthetic medium, had to be consumed completely before conjugation could start. Conjugation was preceded by sexual agglutination. Agglutinability was not constitutive in heterothallic strains. It only deve...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387025

    authors: Egel R

    更新日期:1971-03-01 00:00:00

  • Monoclonal antibodies to plant plasma-membrane antigens.

    abstract::Murine monoclonal antibodies to membrane antigens were generated by immunization with a crude cellular membrane preparation from suspension-cultured cells of Nicotiana glutinosa L. From a panel of thirteen monoclonal antibodies, seven were found to be directed against antigens present on the plasma-membrane by immunof...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391220

    authors: Norman PM,Wingate VP,Fitter MS,Lamb CJ

    更新日期:1986-04-01 00:00:00

  • Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins.

    abstract::Nucellar-derived cell cultures of sour orange (Citrus aurantium L.) proliferate as proembryogenic masses. By a change in the carbon source of the medium from sucrose to glycerol they are induced to undergo synchronous embryogenesis forming embryo initials that develop into globular embryos. The proembryogenic masses r...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00198030

    authors: Gavish H,Vardi A,Fluhr R

    更新日期:1992-03-01 00:00:00

  • Are polyamines involved in the induction and regulation of the Crassulacean acid metabolism?

    abstract::Leaves of plants with Crassulacean acid metabolism (CAM) were analyzed for variation in the content of polyamines in connection with the metabolism of malic acid in the dark and in the light, and with the induction of full-CAM activity. Under conditions (long days) resulting in extremely low CAM activity, young leaves...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385745

    authors: Morel C,Villanueva VR,Queiroz O

    更新日期:1980-10-01 00:00:00

  • Experimentally induced binding of phytochrome to mitochondrial and microsomal fractions in etiolated pea shoots.

    abstract::A brief irradiation with red light of pea (Pisum sativum L.) shoot segments kept at 0° resulted in very rapid binding of both Pr and Pfr to mitochondrial and microsomal fractions. The effect was not far-red reversible. The amount of phytochrome bound to the mitochondrial fraction was proportional to the percentage of ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390699

    authors: Manabe K,Furuya M

    更新日期:1975-01-01 00:00:00