Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots.

Abstract:

MAIN CONCLUSION:The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, has potential allelopathic activity and affects auxin transport. However, little is known about the cellular mechanism of this inhibitory effect of NCS on auxin transport. The present study characterizes the effects of NCS at the cellular level using transgenic Arabidopsis plants harboring the promoters of PIN, in combination with PIN-GFP proteins or AUX1-YFP fusions. NCS treatment caused significant reduction in the abundance of PIN and AUX1 proteins at the plasma membrane (PM). Analysis of the subcellular distribution of PIN and AUX1 proteins in roots revealed that NCS induced the intracellular accumulation of auxin transporters, including PIN2, PIN3, PIN4, PIN7 and AUX1. However, other PM proteins, such as PIP2, BRI1, and low temperature inducible protein 6b (LTI6b), were insensitive to NCS treatment. NCS-induced PIN2 compartments were further defined using endocytic tracer FM 4-64 labeled early endosomes and suggested that this compound affects the endocytosis trafficking of PIN proteins. Furthermore, pharmacological analysis indicated that the brefeldin A (BFA)-insensitive pathway is employed in the cellular effects of NCS on PIN2 trafficking. Although NCS did not alter actin dynamics in vitro, it resulted in the depolymerization of the actin cytoskeleton in vivo. This disruption of actin filaments by NCS subsequently influences the actin-based vesicle motility. Hence, the elucidation of the specific role of NCS is useful for further understanding the mechanisms of allelopathy at the phytohormone levels.

journal_name

Planta

journal_title

Planta

authors

Hu Y,Na X,Li J,Yang L,You J,Liang X,Wang J,Peng L,Bi Y

doi

10.1007/s00425-015-2373-6

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

1349-60

issue

6

eissn

0032-0935

issn

1432-2048

pii

10.1007/s00425-015-2373-6

journal_volume

242

pub_type

杂志文章

相关文献

PLANTA文献大全
  • [Demonstration of a threshold regulation by phytochrome in the photomodulation of longitudinal growth of the hypocotyl of mustard seedlings (Sinapis alba L.)].

    abstract::The inhibition of hypocotyl lengthening in intact mustard seedlings is controlled by two photosensitive systems which can be experimentally separated. 1. Kinetics of the growth response in the dark after red and far-red irradiation (Figs. 1, 2) demonstrate the operation of Pfr in the ground state via a threshold mecha...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385217

    authors: Schopfer P,Oelze-Karow H

    更新日期:1971-06-01 00:00:00

  • Photosynthesis in sugar beet depends on root growth.

    abstract::Sugar-beet plants, germinated in growth cabinets at 20°C and transplanted into the field after 3 weeks, developed much larger roots than plants grown from seed drilled directly into the soil. At the end of the season, the roots of transplants were 39% greater than from drilled seed-an increase of 14 m tons per hectare...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00396118

    authors: Humphries EC,French SA

    更新日期:1969-03-01 00:00:00

  • Effects of (+)-8',8',8'-trifluoroabscisic acid on alpha-amylase expression and sugar accumulation in rice cells.

    abstract::The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on alpha-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Tr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050326

    authors: Kashem MA,Hori H,Itoh K,Hayakawa T,Todoroki Y,Hirai N,Ohigashi H,Mitsui T

    更新日期:1998-07-01 00:00:00

  • The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta.

    abstract::In the genus Petunia, distinct pollination syndromes may have evolved in association with bee-visitation (P. integrifolia spp.) or hawk moth-visitation (P. axillaris spp). We investigated the extent of congruence between floral fragrance and olfactory perception of the hawk moth Manduca sexta. Hawk moth pollinated P. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-1506-8

    authors: Hoballah ME,Stuurman J,Turlings TC,Guerin PM,Connétable S,Kuhlemeier C

    更新日期:2005-09-01 00:00:00

  • Nitrogen redistribution during grain growth in wheat (Triticum aestivum L.) : III. Enzymology and transport of amino acids from senescing flag leaves.

    abstract::The technique of EDTA-enhanced phloem exudation (King and Zeevaart, 1974: Plant Physiol. 53, 96-103) was evaluated with respect to the collection and identification of amino acids exported from senescing wheat leaves. Whilst the characteristics of the exudate collected conform with many of the accepted properties of p...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386538

    authors: Simpson RJ,Dalling MJ

    更新日期:1981-05-01 00:00:00

  • Site-specific phosphorylation of L-form starch phosphorylase by the protein kinase activity from sweet potato roots.

    abstract::A 78-amino acid insertion (L78) is found in the low-affinity type (L-form) of starch phosphorylase (L-SP, EC 2.4.1.1). This insertion blocks the starch-binding site on the L-SP molecule, and it decreases the binding affinity of L-SP toward starch. The computational analysis of the amino acid sequence on L78 predicts s...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-0103-1

    authors: Young GH,Chen HM,Lin CT,Tseng KC,Wu JS,Juang RH

    更新日期:2006-02-01 00:00:00

  • Phytochrome quantitation in crude extracts of Avena by enzyme-linked immunosorbent assay with monoclonal antibodies.

    abstract::An enzyme-linked immunosorbent assay (ELISA), which uses both rabbit polyclonal and mouse monoclonal antibodies to phytochrome, has been adapted for quantitation of phytochrome in crude plant extracts. The assay has a detection limit of about 100 pg phytochrome (<1 fmol of monomer) and can be completed within 10 h. No...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00409143

    authors: Shimazaki Y,Cordonnier MM,Pratt LH

    更新日期:1983-12-01 00:00:00

  • Influence of winter and summer growth conditions on leaf membrane lipids of Pisum sativum L.

    abstract::The lipid composition and degree of unsaturation of fatty acids has been measured for membranes isolated from leaves of Pisum sativum grown under either summer or winter conditions. Although the lipid-class composition was not significantly changed for the two growth conditions the degree of unsaturation of the fatty ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00405185

    authors: Chapman DJ,De-Felice J,Barber J

    更新日期:1983-04-01 00:00:00

  • Involvement of auxin distribution in root nodule development of Lotus japonicus.

    abstract::The symbiosis between legume plants and rhizobia causes the development of new organs, nodules which function as an apparatus for nitrogen fixation. In this study, the roles of auxin in nodule development in Lotus japonicus have been demonstrated using molecular genetic tools and auxin inhibitors. The expression of an...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1385-0

    authors: Takanashi K,Sugiyama A,Yazaki K

    更新日期:2011-07-01 00:00:00

  • Fixation patterns of (14)C within developing leaves of eastern cottonwood.

    abstract::Individual leaves of eastern cottonwood (Populus deltoides), representing an ontogenetic series from leaf plastochron index 0.0 to 8.0, were fed (14)CO2 photosynthetically and then harvested at times ranging from 15 to 1440 min. The lamina of each fed leaf was sectioned from tip to base into 5 parts, and each part was...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386392

    authors: Larson PR,Isebrands JG,Dickson RE

    更新日期:1972-12-01 00:00:00

  • Aspects of phytochrome decay in etiolated seedlings under continuous Illumination.

    abstract::The rate of total phytochrome decay in the dicotyledons Amaranthus caudatus, Mirabilis jalapa and Pisum sativum under continuous illumination with red, incandescent, and blue light depends on the PFR/Ptotal maintained by each source. Amaranthus is an exception to this in that there is a deviation from firstorder decay...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386614

    authors: Kendrick RE

    更新日期:1972-12-01 00:00:00

  • MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana.

    abstract::Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacumL.) and banana [( Musaspp. cv. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0918-y

    authors: Chakrabarti A,Ganapathi TR,Mukherjee PK,Bapat VA

    更新日期:2003-02-01 00:00:00

  • Scanning electron-microscopy of abscission-zone surfaces of Valencia-orange fruit.

    abstract::The scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of Valencia orange. Separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. The most noticeable differences are the lessened amount of tearing in pr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390215

    authors: Rogers BJ

    更新日期:1971-12-01 00:00:00

  • Stimulation of membrane-associated polysaccharide synthetases by a membrane potential in developing cotton fibers.

    abstract::Conditions which induce a transmembrane electrical potential, positive with respect to the inside of membrane vesicles, result in a substantial (4-12-fold) stimulation of the activity of membrane-associated β-glucan synthetases in a membrane preparation derived from the developing cotton (Gossypium hirsutum L.) fiber....

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388260

    authors: Bacic A,Delmer DP

    更新日期:1981-07-01 00:00:00

  • Genotypic and heat stress effects on leaf cuticles of field pea using ATR-FTIR spectroscopy.

    abstract:MAIN CONCLUSION:ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress. Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy co...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-3025-4

    authors: Liu N,Karunakaran C,Lahlali R,Warkentin T,Bueckert RA

    更新日期:2019-02-01 00:00:00

  • Effects of pH and deuterium oxide on the heat-inactivation temperature of chloroplasts.

    abstract::The inactivation temperature for Hill activity and for the long-lived delayed fluorescence of isolated Pisum sativum L. chloroplasts was found to depend on pH, the maximal value being in the pH region 5-7. Salts increase the inactivation temperature by 4-7°C. Effects of D2O and some other substances that modify the th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00402854

    authors: Venediktov PS,Krivoshejeva AA

    更新日期:1984-03-01 00:00:00

  • Endogenous hydrogen peroxide is a key factor in the yeast extract-induced activation of biphenyl biosynthesis in cell cultures of Sorbus aucuparia.

    abstract::Biphenyls are unique phytoalexins produced by plants belonging to Pyrinae, a subtribe of the economically important Rosaceae family. The formation of aucuparin, a well-known biphenyl, is induced by yeast extract (YE) in cell cultures of Sorbus aucuparia. However, the molecular mechanism underlying YE-induced activatio...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1545-2

    authors: Qiu X,Lei C,Huang L,Li X,Hao H,Du Z,Wang H,Ye H,Beerhues L,Liu B

    更新日期:2012-01-01 00:00:00

  • Biosynthesis of (1,3)(1,4)-beta-glucan and (1,3)-beta-glucan in barley (Hordeum vulgare L.). Properties of the membrane-bound glucan synthases.

    abstract::Mixed membrane preparations from the coleoptiles and first leaves of young barley (Hordeum vulgare L. cv. Triumph) plants catalysed the synthesis of 55% methanol-insoluble labelled material from UDP-[U-14C]glucose, the main components of which were identified as (1,3)(1,4)-beta- and (1,3)-beta-D-glucans. The membrane ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00202589

    authors: Becker M,Vincent C,Reid JS

    更新日期:1995-01-01 00:00:00

  • Glutamine synthetase in Scots pine seedlings and its control by blue light and light absorbed by phytochrome.

    abstract::The appearance of glutamine synthetase (GS. EC 6.3.1.2) in response to light and nitrogen (NO 3 (-) , NH 4 (+) ) was studied in the organs (roots, hypocotyl, cotyledonary whorl) of the Scots pine (Pinus sylvestris L.) seedling. Although GS activity was found to be mainly (> 80%) located in the whorl where it increased...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00192807

    authors: Elmlinger MW,Mohr H

    更新日期:1992-10-01 00:00:00

  • Mode of glucan degradation by purified phosphorylase forms from spinach leaves.

    abstract::The glucan specifity of the purified chloroplast and non-chloroplast forms of α-1,4-glucan phosphorylase (EC 2.4.1.1) from spinach leaves (Steup and E. Latzko (1979), Planta 145, 69-75) was investigated. Phosphorolysis by the two enzymes was studied using a series of linear maltodextrins (degree of polymerization ≦11)...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384254

    authors: Steup M,Schächtele C

    更新日期:1981-12-01 00:00:00

  • Role of cytokinin in the regulation of root gravitropism.

    abstract::The models explaining root gravitropism propose that the growth response of plants to gravity is regulated by asymmetric distribution of auxin (indole-3-acetic acid, IAA). Since cytokinin has a negative regulatory role in root growth, we suspected that it might function as an inhibitor of tropic root elongation during...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1381-8

    authors: Aloni R,Langhans M,Aloni E,Ullrich CI

    更新日期:2004-11-01 00:00:00

  • The effects of photoinhibition on the photosynthetic light-response curve of green plant cells (Chlamydomonas reinhardtii).

    abstract::The photosynthetic response to light can be accurately defined in terms of (1) the initial slope (quantum yield); (2) the asymptote (light-saturated rate); (3) the convexity (rate of bending); and (4) the intercept (dark respiration). The effects of photoinhibition [which damages the reaction centre of photosystem II ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197105

    authors: Leverenz JW,Falk S,Pilström CM,Samuelsson G

    更新日期:1990-09-01 00:00:00

  • A method for high-frequency intergeneric fusion of plant protoplasts.

    abstract::Protoplasts of Vicia hajastana Grossh. obtained from suspension-culture cells and Pisum sativum L. obtained from leaves adhered tightly to each other in concentrated solutions of high-molecular-weight polyethylene glycol (PEG). The adhesion occurred non-specifically between the free protoplasts from the same species a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388618

    authors: Kao KN,Michayluk MR

    更新日期:1974-12-01 00:00:00

  • The NADPH-protochlorophyllide oxidoreductase is the major protein constituent of prolamellar bodies in wheat (Triticum aestivum L.).

    abstract::A fraction of highly purified prolamellar bodies was isolated from etioplasts of wheat (Triticum aestivum L. cv. Starke II, Weibull), as previously described by Ryberg and Sundqvist (1982, Physiol. Plant., 56, 125-132). Studies on the protein composition revealed that only one major polypeptide of an apparent molecula...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00402952

    authors: Dehesh K,Ryberg M

    更新日期:1985-06-01 00:00:00

  • Glutamine synthetase in the chloroplasts of Vicia faba.

    abstract::A glutamine synthetase has been localised in the chloroplasts of Vicia faba. The enzyme has requirements for Mg(2+) and ATP in the biosynthetic reaction and in addition will catalyse a γ-glutamyl transferase reaction in the presence of Mn(2+) and arsenate. The enzyme is inhibited by AMP, CTP, glycine and alanine. Thes...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385111

    authors: Haystead A

    更新日期:1973-09-01 00:00:00

  • Small tandem target mimic-mediated blockage of microRNA858 induces anthocyanin accumulation in tomato.

    abstract:MAIN CONCLUSION:Our work strongly suggests that microRNA858 regulates anthocyanin biosynthesis in tomato by modulating the expression of two R2R3 MYB transcription factors, underscoring the importance of microRNAs in the gene regulatory network controlling specialized metabolism in plants. The biological functions of m...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-015-2305-5

    authors: Jia X,Shen J,Liu H,Li F,Ding N,Gao C,Pattanaik S,Patra B,Li R,Yuan L

    更新日期:2015-07-01 00:00:00

  • Localization and activity of various peptidases in germinating barley.

    abstract::Germinating barley grains contain at least eight different peptidases: three carboxypeptidase (pH optima 4.8, 5.2, and 5.7), three aminopeptidases which act on aminoacyl-β-naphthylamides (pH opitima in the hydrolysis of di- and tripeptides at pH 5.8-6.5), and two peptidases which hydrolyse Ala-Gly and Leu-Tyr optimall...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386993

    authors: Mikola J,Kolehmainen L

    更新日期:1972-06-01 00:00:00

  • Apple latent spherical virus vector-induced flowering for shortening the juvenile phase in Japanese gentian and lisianthus plants.

    abstract:MAIN CONCLUSION:Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-016-2498-2

    authors: Fekih R,Yamagishi N,Yoshikawa N

    更新日期:2016-07-01 00:00:00

  • Concanavalin A binding sites on the plasma membrane of leek stem protoplasts.

    abstract::The binding of concanavalin A (con A) to leek (Allium porrum L.) stem protoplasts has been investigated using sequential treatment with con A and haemocyanin and using con A covalently linked to ferritin. Prefixed protoplasts were evenly labelled. Unfixed protoplasts showed a clustered distribution of label. Low tempe...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388761

    authors: Williamson FA

    更新日期:1979-01-01 00:00:00

  • Cell death and salicylate- and jasmonate-dependent stress responses in Arabidopsis are controlled by single cet genes.

    abstract::The jasmonic acid (JA)-dependent regulation of the Thi2.1 gene had previously been exploited for setting up a genetic screen for the isolation of signal transduction mutants of Arabidopsis thaliana (L.) Heynh. that constitutively express the thionin gene. Several cet mutants had been isolated which showed a Constituti...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0907-1

    authors: Nibbe M,Hilpert B,Wasternack C,Miersch O,Apel K

    更新日期:2002-11-01 00:00:00