Glutamine synthetase in the chloroplasts of Vicia faba.

Abstract:

:A glutamine synthetase has been localised in the chloroplasts of Vicia faba. The enzyme has requirements for Mg(2+) and ATP in the biosynthetic reaction and in addition will catalyse a γ-glutamyl transferase reaction in the presence of Mn(2+) and arsenate. The enzyme is inhibited by AMP, CTP, glycine and alanine. These results are discussed in relation to the possible chloroplastic synthesis of nucleotide bases. Estimations of glutamine amide-2-oxoglutarate amino transferase (oxido-reductase) have demonstrated only low levels of activity in the chloroplast extracts. This enzyme is generally active in organisms where GS has an assimilary role. It is coneluded that glutamine synthetase has a biosynthetic and not an assimilatory role in the chloroplast.

journal_name

Planta

journal_title

Planta

authors

Haystead A

doi

10.1007/BF00385111

subject

Has Abstract

pub_date

1973-09-01 00:00:00

pages

271-4

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

111

pub_type

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