Abstract:
:Wild-type (Ph1) and adenine-requiring (Ad1) cell lines of Datura innoxia Mill. were used in experiments to evaluate arsenate as a growth-lethal compound and its use as a counterselection agent. These experiments led to the devising of methods for the recovery of Ph1 and Ad1 cells after arsenate treatment when plated at low density on feeder plates. The modified counterselection technique was then used to isolate three new auxotrophs from mutagenized suspensions of Ph1 cells, two of which were partially characterized. One, C18, requires casein hydrolysate for growth and lacks an active nitrate reductase; the other, JM3, will grow only when the medium contains threonine.
journal_name
Plantajournal_title
Plantaauthors
Horsch RB,King Jdoi
10.1007/BF00392866subject
Has Abstractpub_date
1984-02-01 00:00:00pages
168-73issue
2eissn
0032-0935issn
1432-2048journal_volume
160pub_type
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