Cardenolide 16'-O-glucohydrolase from Digitalis lanata. Purification and characterization.


:A three-step chromatographic procedure was developed for purification of cardenolide 16'-O-glucohydrolase (CGH) from Digitalis lanata Ehrh. leaves, including Phenyl-Sepharose hydrophobic interaction chromatography followed by SP-Sepharose cation exchange and Q-Sepharose anion-exchange chromatography. Starting with acetone dry powder the purification resulted in an 760-fold enrichment of CGH. Molecular weight, substrate specificity, pH optimum and temperature stability of CGH were determined. Antibodies against CGH were prepared in rabbits. The SDS gel electrophoresis of protein extracts from leaves of D. lanata and other D. species showed bands at 70 kDa and 36 kDa reacting with the antibodies. The 70-kDa protein is the main protein stained with CGH antibodies in freshly prepared extracts of D. lanata. It may represent undegraded CGH. The 36-kDa protein is enriched in aged CGH preparations. It is probably a degradation product. Proteins related to 70-kDa and 36-kDa bands also occur in crude protein preparations from leaves of D. heywoodii P. et M. Silva, D. mariana Boiss., D. purpurea L., and D. thapsi L. indicating that CGH is also present in these species. Purified CGH was digested with proteases V8 and Lys-C and the resulting fragments obtained were sequenced. One fragment had the typical amino-acid sequence of the catalytic center of family-1 glycosyl hydrolases (EC 3.2.1.x). Cardenolide 16'-O-glucohydrolase, like the other members of this enzyme family, appeared to have a glutamic acid residue directly involved in glycosidic bond cleavage as a nucleophile.






Schöniger R,Lindemann P,Grimm R,Eckerskorn C,Luckner M




Has Abstract


1998-07-01 00:00:00
















  • Germination-specific lipid transfer protein cDNAs in Brassica napus L.

    abstract::Three cDNA clones encoding lipid transfer proteins (LTPs) were isolated by applying the rapid amplification of cDNA ends (RACE) protocol to imbibed seeds and germinating seedlings Brassica napus. The deduced amino-acid sequences show a great degree of homology and they exhibit the common features shared by all LTPs. T...


    pub_type: 杂志文章


    authors: Soufleri IA,Vergnolle C,Miginiac E,Kader JC

    更新日期:1996-01-01 00:00:00

  • [Effect of terpenoid derivatives (helminthosporol, helminthosporic acid, dihydrohelminthosporic acid, and steviol) on antheridinum induction in Anemia phyllitidis].

    abstract::Helminthosporol, helminthosporic acid, dihydrohelminthosporic acid, and steviol have been tested for their ability to induce antheridium formation in Anemia phyllitidis. All helminthosporol derivatives posses a limited but real activity. Steviol proved to be inactive.All the substance tested have a significant effect ...


    pub_type: 杂志文章


    authors: Schraudolf H

    更新日期:1967-06-01 00:00:00

  • Isolation and characterization of a jacalin-related mannose-binding lectin from salt-stressed rice (Oryza sativa) plants.

    abstract::A novel plant lectin was isolated from salt-stressed rice (Oryza sativa L.) plants and partially characterized. The lectin occurs as a natural mixture of two closely related isoforms consisting of two identical non-covalently linked subunits of 15 kDa. Both isoforms are best inhibited by mannose and exhibit potent mit...


    pub_type: 杂志文章


    authors: Zhang W,Peumans WJ,Barre A,Astoul CH,Rovira P,Rougé P,Proost P,Truffa-Bachi P,Jalali AA,Van Damme EJ

    更新日期:2000-05-01 00:00:00

  • Plasmatubules: an alternative to transfer cells?

    abstract::Tubular evaginations of the plasmalemma of the scutellar epithelial cells of barley are described. The evaginations are similar to those present at other sites where solute flux occurs for a limited period only and wall development of the transfer-cell form has not occured. Differential uptake of the fluorescent dyes ...


    pub_type: 杂志文章


    authors: Harris N,Oparka KJ,Walker-Smith DJ

    更新日期:1982-12-01 00:00:00

  • Reorganization of wheat and rye genomes in octoploid triticale (× Triticosecale).

    abstract:MAIN CONCLUSION:The analysis of early generations of triticale showed numerous rearrangements of the genome. Complexed transformation included loss of chromosomes, t-heterochromatin content changes and the emergence of retrotransposons in new locations. This study investigated certain aspects of genomic transformations...


    pub_type: 杂志文章


    authors: Kalinka A,Achrem M

    更新日期:2018-04-01 00:00:00

  • "Arabidobrassica": A novel plant obtained by protoplast fusion.

    abstract::Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in ...


    pub_type: 杂志文章


    authors: Gleba YY,Hoffmann F

    更新日期:1980-07-01 00:00:00

  • Extraction of hydroxyproline-containing proteins and pectic substances from cell walls of growing and non-growing mung bean hypocotyl segments.

    abstract::Elongating upper and non-elongating lower segments of mung bean hypocotyls (Phaseolus aureus Roxb.) were compared in their ability to incorporate labelled polymers into their cell walls when fed with [(14)C]proline. The non-growing segments incorporated more amino acids into the walls and showed a higher degree of hyd...


    pub_type: 杂志文章


    authors: Bailey RW,Kauss H

    更新日期:1974-09-01 00:00:00

  • Phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase: Characterisation of the concomitant changes in enzyme activities in illuminated potato tuber discs.

    abstract::Phenylalanine ammonia-lyase (PAL, EC and cinnamic acid 4-hydroxylase (CA4H, EC undergo concomitant increases in activity after a 2 h lag following disc preparation and illumination. The increases in PAL and CA4H activity can be inhibited by actinomycin-D, cordycepin and cycloheximide. It is demons...


    pub_type: 杂志文章


    authors: Lamb CJ

    更新日期:1977-01-01 00:00:00

  • The evolutionary landscape of PRC1 core components in green lineage.

    abstract:MAIN CONCLUSION:The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. It is ...


    pub_type: 杂志文章,评审


    authors: Chen DH,Huang Y,Ruan Y,Shen WH

    更新日期:2016-04-01 00:00:00

  • The microtubular cytoskeleton during development of the zygote, proembryo and free-nuclear endosperm inArabidopsis thaliana (L.) Heynh.

    abstract::The microtubular cytoskeleton has been studied during development of the zygote, proembryo and free-nuclear endosperm inA. thaliana using immunofluorescence localization of tubulin in enzymatically isolated material. Abundant micro tubules (MTs) are found throughout proembryogenesis. Microtubules in the coenocytic end...


    pub_type: 杂志文章


    authors: Webb MC,Gunning BE

    更新日期:1991-06-01 00:00:00

  • MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana.

    abstract::Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacumL.) and banana [( Musaspp. cv. ...


    pub_type: 杂志文章


    authors: Chakrabarti A,Ganapathi TR,Mukherjee PK,Bapat VA

    更新日期:2003-02-01 00:00:00

  • Inhibition of cell division in a moss protonema by some cytokinin isomers.

    abstract::The cytokinin isomers, 1- and 9-isopentenyladenine, are very active inhibitors of cell division in the protonema of the moss Ceratodon purpureus. They also abolish the N(6)-isopentenyladenine-induced stimulation of cell divisions in this material whereas they are inactive on the induction of gametophore buds in moss p...


    pub_type: 杂志文章


    authors: Szweykowska A,Korcz I

    更新日期:1972-09-01 00:00:00

  • Volatiles modulate the development of plant pathogenic rust fungi.

    abstract::Rust fungi are obligate biotrophic pathogens that differentiate a series of specialized cells to establish infection. One of these cells, the haustorium, which serves to absorb nutrients from living host cells, normally develops only in planta. Here, we show that the rust fungus Uromyces fabae (Pers.) Schroet. stimula...


    pub_type: 杂志文章


    authors: Mendgen K,Wirsel SG,Jux A,Hoffmann J,Boland W

    更新日期:2006-11-01 00:00:00

  • Scanning electron-microscopy of abscission-zone surfaces of Valencia-orange fruit.

    abstract::The scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of Valencia orange. Separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. The most noticeable differences are the lessened amount of tearing in pr...


    pub_type: 杂志文章


    authors: Rogers BJ

    更新日期:1971-12-01 00:00:00

  • Molecular basis for multiple resistance to acetolactate synthase-inhibiting herbicides and atrazine in Amaranthus blitoides (prostrate pigweed).

    abstract::Amaranthus blitoides S. Watson (prostrate pigweed) populations resistant to acetolactate synthase (ALS; EC herbicides and triazines (SuR/TR) were found in Israel. The Ganot population was 6- to 790-fold more resistant to ALS inhibitors than the wild type due to an altered target site. Molecular an...


    pub_type: 杂志文章


    authors: Sibony M,Rubin B

    更新日期:2003-04-01 00:00:00

  • Non-hydraulic signals from maize roots in drying soil: inhibition of leaf elongation but not stomatal conductance.

    abstract::Conditions of soil drying and plant growth that lead to non-hydraulic inhibition of leaf elongation and stomatal conductance in maize (Zea mays L.) were investigated using plants grown with their root systems divided between two containers. The soil in one container was allowed to dry while the other container was kep...


    pub_type: 杂志文章


    authors: Saab IN,Sharp RE

    更新日期:1989-11-01 00:00:00

  • Absence of turnover and futile cycling of sucrose in leaves of Lolium temulentum L.: implications for metabolic compartmentation.

    abstract::To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC, in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, ea...


    pub_type: 杂志文章


    authors: Cairns AJ,Gallagher JA

    更新日期:2004-09-01 00:00:00

  • Biochemical analysis of elastic and rigid cuticles of Cirsium horridulum.

    abstract::The cuticle is a complex structure of soluble lipids, lipid polymers and polysaccharides. In addition to its functions to reduce water loss and provide a protective barrier, its mechanical properties may be significant to plant growth and development. We investigated the cuticle of Cirsium horridulum Michx. because of...


    pub_type: 杂志文章


    authors: Marga F,Pesacreta TC,Hasenstein KH

    更新日期:2001-10-01 00:00:00

  • Glycollate formation during the photoassimilation of acetate by Chlorella.

    abstract::When (3)H-(14)C-acetate was supplied to Chlorella pyrenoidosa in the light, glycollic acid became rapidly labelled with tritium and (14)C. The [Formula: see text]ratio of glycollate was 10, whilst the ratio was 4 in the acetate added. Both (3)H and (14)C from acetate were present in glycollate before they were present...


    pub_type: 杂志文章


    authors: Merrett MJ,Goulding KH

    更新日期:1967-09-01 00:00:00

  • VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis.

    abstract:MAIN CONCLUSION:Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate...


    pub_type: 杂志文章


    authors: Song C,Lee J,Kim T,Hong JC,Lim CO

    更新日期:2018-06-01 00:00:00

  • Relationships between xanthoxin, phototropism, and elongation growth in the sunflower seedling Helianthus annuus L.

    abstract::For phototropic curvature of a green sunflower seedling, only the hypocotyl has to be illuminated; the tip and cotyledons are not involved in stimulus perception. The etiolated seedling is phototropically insensitive, illumination of only the hypocotyl renders it sensitive. It is concluded that the photoreceptor is lo...


    pub_type: 杂志文章


    authors: Franssen JM,Bruinsma J

    更新日期:1981-04-01 00:00:00

  • A new interpretation of plasmodesmatal ultrastructure.

    abstract::It is shown that simple, unbranched, plasmodesmata between young xylem ray cells of willow have no direct intercellular continuity apart from the plasmalemma which limits the cytoplasm and lines the plasmodesmatal canal. Each plasmodesma is traversed by a 200 Å diameter tubule (the desmotubule) which has a wall with p...


    pub_type: 杂志文章


    authors: Robards AW

    更新日期:1968-09-01 00:00:00

  • Photocontrol of hypocotyl elongation in light-grown Cucumis sativus L. : The end-of-day response to phytochrome.

    abstract::The control by phytochrome of hypocotyl elongation of light-grown Cucumis sativus L. after a white-light period was examined. The farred-absorbing form of phytochrome inhibits hypocotyl elongation. The response to phytochrome photostationary state (ϕ) is not linear; all values of ϕ from 0.004 to 0.13 promote growth ma...


    pub_type: 杂志文章


    authors: Gaba V,Black M

    更新日期:1985-05-01 00:00:00

  • [Influence of the cell metabolism on the pigment composition in ageing cultures of Euglena gracilis].

    abstract::The qualitative and quantitative composition of the carotenoids of young cells of Euglena gracilis strain 1224-5/9 corresponded to that of the "Z"-strain.The frequently observed yellow-reddish discolouration of ageing cells was found to be caused by a heavy breakdown of the chlorophylls and not by an increased synthes...


    pub_type: 杂志文章


    authors: Schwenker U

    更新日期:1971-06-01 00:00:00

  • The effect of chemical stress on the polypeptide composition of the intercellular fluid of barley leaves.

    abstract::The effect of chemical stress on the polypeptide composition of the intercellular fluid of barley (Hordeum vulgare L.) and tomato (Lycopersicon esculentum Mill.) leaves has been studied. In some dicotyledonous plant species, including tomato, exposure to chemical stress leads to the denovo synthesis of intercellular p...


    pub_type: 杂志文章


    authors: Fischer R,Behnke S,Apel K

    更新日期:1989-05-01 00:00:00

  • Spatial and temporal changes in endogenous cytokinins in developing pea roots.

    abstract::Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Ra...


    pub_type: 杂志文章


    authors: Stirk WA,Novák O,Václavíková K,Tarkowski P,Strnad M,van Staden J

    更新日期:2008-05-01 00:00:00

  • Induction of multinucleation by beta-glucosyl Yariv reagent in regenerated cells from Marchantia polymorpha protoplasts and involvement of arabinogalactan proteins in cell plate formation.

    abstract::Arabinogalactan proteins (AGPs) are abundant plant cell surface proteoglycans widely distributed in plant species. Since high concentrations of beta-glucosyl Yariv reagent (betaglcY), which binds selectively to AGPs, inhibited cell division of protoplast-regenerated cells of the liverwort Marchantia polymorpha L. (Shi...


    pub_type: 杂志文章


    authors: Shibaya T,Sugawara Y

    更新日期:2009-08-01 00:00:00

  • The structure of the salt gland of Aegiceras corniculatum.

    abstract::The salt glands of Aegiceras corniculatum have been examined by light- and electron-microscope techniques. A gland consists of a large number of abutting secretory cells and a single, large basal cell. The secretory cells and basal cell are joined by well defined plasmodesmata. The glandular cuticle shows differences ...


    pub_type: 杂志文章


    authors: Cardale S,Field CD

    更新日期:1971-09-01 00:00:00

  • Cytochemical localization of phosphatase in barley aleurone cells: The pathway of gibberellic-acid-induced enzyme release.

    abstract::Acid phosphatase has been localized by cytochemical techniques in aleurone layers of dry barley (Hordeum vulgare L.) grains, in imbibed half-grains and in isolated layers treated with and without gibberellic acid (GA3). A major fraction of the enzyme activity is located in the cell walls. During imbibition and incubat...


    pub_type: 杂志文章


    authors: Ashford AE,Jacobsen JV

    更新日期:1974-01-01 00:00:00

  • Linking jasmonates with vitamin E accumulation in plants: a case study in the Mediterranean shrub Cistus albidus L.

    abstract:MAIN CONCLUSION:Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional ...


    pub_type: 杂志文章


    authors: Casadesús A,Bouchikh R,Pérez-Llorca M,Munné-Bosch S

    更新日期:2021-01-18 00:00:00