Cardenolide 16'-O-glucohydrolase from Digitalis lanata. Purification and characterization.


:A three-step chromatographic procedure was developed for purification of cardenolide 16'-O-glucohydrolase (CGH) from Digitalis lanata Ehrh. leaves, including Phenyl-Sepharose hydrophobic interaction chromatography followed by SP-Sepharose cation exchange and Q-Sepharose anion-exchange chromatography. Starting with acetone dry powder the purification resulted in an 760-fold enrichment of CGH. Molecular weight, substrate specificity, pH optimum and temperature stability of CGH were determined. Antibodies against CGH were prepared in rabbits. The SDS gel electrophoresis of protein extracts from leaves of D. lanata and other D. species showed bands at 70 kDa and 36 kDa reacting with the antibodies. The 70-kDa protein is the main protein stained with CGH antibodies in freshly prepared extracts of D. lanata. It may represent undegraded CGH. The 36-kDa protein is enriched in aged CGH preparations. It is probably a degradation product. Proteins related to 70-kDa and 36-kDa bands also occur in crude protein preparations from leaves of D. heywoodii P. et M. Silva, D. mariana Boiss., D. purpurea L., and D. thapsi L. indicating that CGH is also present in these species. Purified CGH was digested with proteases V8 and Lys-C and the resulting fragments obtained were sequenced. One fragment had the typical amino-acid sequence of the catalytic center of family-1 glycosyl hydrolases (EC 3.2.1.x). Cardenolide 16'-O-glucohydrolase, like the other members of this enzyme family, appeared to have a glutamic acid residue directly involved in glycosidic bond cleavage as a nucleophile.






Schöniger R,Lindemann P,Grimm R,Eckerskorn C,Luckner M




Has Abstract


1998-07-01 00:00:00
















  • Periodicity of response to abscisic acid in lateral buds of willow (Salix viminalis L.).

    abstract::Aseptically cultured lateral buds of Salix viminalis L. collected from field-grown trees exhibited a clear periodicity in their ability to respond to exogenous abscisic acid (ABA). Buds were kept unopened by ABA only when the plants were dormant or entering dormancy. Short days alone did not induce bud dormancy in pot...


    pub_type: 杂志文章


    authors: Barros RS,Neill SJ

    更新日期:1986-09-01 00:00:00

  • Ovary-drip transformation: a simple method for directly generating vector- and marker-free transgenic maize (Zea mays L.) with a linear GFP cassette transformation.

    abstract::The presence of selectable marker genes and vector backbone sequences has affected the safe assessment of transgenic plants. In this study, the ovary-drip method for directly generating vector- and selectable marker-free transgenic plants was described, by which maize was transformed with a linear GFP cassette (Ubi-GF...


    pub_type: 杂志文章


    authors: Yang A,Su Q,An L

    更新日期:2009-03-01 00:00:00

  • Expression of the ethylene response factor gene TSRF1 enhances abscisic acid responses during seedling development in tobacco.

    abstract::Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-...


    pub_type: 杂志文章


    authors: Zhang H,Yang Y,Zhang Z,Chen J,Wang XC,Huang R

    更新日期:2008-10-01 00:00:00

  • Detection and preliminary identification of endogenous antitranspirants in water-stressed Sorghum plants.

    abstract::Sorghum plants that had been subjected to different degrees of water stress were examined for the occurrence of endogenous compounds capable of inducing stomatal closure, i.e. "antitranspirants". Acidic extracts contained increased amounts of abscisic acid (ABA) as the amount of stress increased, but another highly ac...


    pub_type: 杂志文章


    authors: Ogunkanmi AB,Wellbern AR,Mansfield TA

    更新日期:1974-12-01 00:00:00

  • Onset of desiccation tolerance during development of the barley embryo.

    abstract::We have investigated events which take place in the developing barley (Hordeum vulgare L.) embryo during its acquisition of desiccation tolerance. Excised embryos are capable of precocious germination as early as 8 d after pollination (DAP). At this age, however, they are not capable of resisting a desiccation treatme...


    pub_type: 杂志文章


    authors: Bartels D,Singh M,Salamini F

    更新日期:1988-10-01 00:00:00

  • Regulation and activation of phytoene synthase, a key enzyme in carotenoid biosynthesis, during photomorphogenesis.

    abstract::During photomorphogenesis in higher plants, a coordinated increase occurs in the chlorophyll and carotenoid contents. The carotenoid level is under phytochrome control, as reflected by the light regulation of the mRNA level of phytoene synthase (PSY), the first enzyme in the carotenoid biosynthetic pathway. We investi...


    pub_type: 杂志文章


    authors: Welsch R,Beyer P,Hugueney P,Kleinig H,von Lintig J

    更新日期:2000-11-01 00:00:00

  • An auxin-responsive 1-aminocyclopropane-1-carboxylate synthase is responsible for differential ethylene production in gravistimulated Antirrhinum majus L. flower stems.

    abstract::The regulation of gravistimulation-induced ethylene production and its role in gravitropic bending was studied in Antirrhinum majus L. cut flower stems. Gravistimulation increased ethylene production in both lower and upper halves of the stems with much higher levels observed in the lower half. Expression patterns of ...


    pub_type: 杂志文章


    authors: Woltering EJ,Balk PA,Nijenhuis-Devries MA,Faivre M,Ruys G,Somhorst D,Philosoph-Hadas S,Friedman H

    更新日期:2005-01-01 00:00:00

  • Chloroplast actin filaments organize meshwork on the photorelocated chloroplasts in the moss Physcomitrella patens.

    abstract::Cytoskeleton dynamics during phototropin-dependent chloroplast photorelocation movement was analyzed in protonemal cells of actin- and microtubule-visualized lines of Physcomitrella patens expressing GFP- or tdTomato-talin and GFP-tubulin. Using newly developed epi- and trans-microbeam irradiation systems that permit ...


    pub_type: 杂志文章


    authors: Yamashita H,Sato Y,Kanegae T,Kagawa T,Wada M,Kadota A

    更新日期:2011-02-01 00:00:00

  • Variability of chromosomal DNA contents in maize (Zea mays L.) inbred and hybrid lines.

    abstract::The flow karyotypes of different maize (Zea mays L.) inbred and hybrid lines were analyzed. The accumulation and isolation of large quantities of high-quality metaphase chromosomes from root tips was achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synch...


    pub_type: 杂志文章


    authors: Lee JH,Arumuganathan K,Kaeppler SM,Park SW,Kim KY,Chung YS,Kim DH,Fukui K

    更新日期:2002-08-01 00:00:00

  • Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots.

    abstract:MAIN CONCLUSION:The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated fro...


    pub_type: 杂志文章


    authors: Hu Y,Na X,Li J,Yang L,You J,Liang X,Wang J,Peng L,Bi Y

    更新日期:2015-12-01 00:00:00

  • High-temperature damage and acclimation of the photosynthetic apparatus : I. Temperature sensitivity of some photosynthetic parameters of chloroplasts isolated from acclimated and non-acclimated bean leaves.

    abstract::The thermosensitivity of delayed fluorescence, the relative values of variable chlorophyll fluorescence and the degree of quenching of 9-aminoacridine fluorescene were studied in the chloroplasts from heat-acclimated and non-acclimated (treated 6 h at 52,5°C) young bean plants. The temperature sensitivity of each para...


    pub_type: 杂志文章


    authors: Yordanov I,Goltsev V,Stoyanova T,Venediktov P

    更新日期:1987-04-01 00:00:00

  • Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line.

    abstract::A heat-tolerant maize (Zea mays L.) line, ZPBL 1304, synthesizes a unique set of five heat-shock polypeptides of 45 kDa. Previous studies suggested that these polypeptides might play a role in the development of thermotolerance in maize (Ristic et al., 1996, J. Plant Physiol. 149:424-432; Ristic et al., 1998, J. Plant...


    pub_type: 杂志文章


    authors: Bhadula SK,Elthon TE,Habben JE,Helentjaris TG,Jiao S,Ristic Z

    更新日期:2001-02-01 00:00:00

  • Cytoplasmic free calcium in Riccia fluitans L. and Zea mays L.: Interaction of Ca(2+) and pH?

    abstract::In cells of Zea mays (root hairs, coleoptiles) and Riccia fluitans (rhizoids, thalli) intracellular Ca(2+) and pH have been measured with double-barrelled microelectrodes. Free Ca(2+) activities of 109-187 nM (Riccia rhizoids), 94-160 nM (Riccia thalli), 145-231 nM (Zea root hairs), 84-143 nM (Zea coleoptiles) were fo...


    pub_type: 杂志文章


    authors: Felle H

    更新日期:1988-11-01 00:00:00

  • The light-induced reduction of the gravitropic growth-orientation of seedlings of Arabidopsis thaliana (L.) Heynh. is a photomorphogenic response mediated synergistically by the far-red-absorbing forms of phytochromes A and B.

    abstract::Hypocotyls of dark-grown seedlings of Arabidosis thaliana exhibit a strong negative gravitropism, which is reduced by red and also by long-wavelength, far-red light treatments. Light treatments using phytochrome A (phyA)- and phytochrome B (phyB)-deficient mutants showed that this response is controlled by phyB in a r...


    pub_type: 杂志文章


    authors: Poppe C,Hangarter RP,Sharrock RA,Nagy F,Schäfer E

    更新日期:1996-01-01 00:00:00

  • Variation in endoplasmic-reticulum-associated glycoproteins of carrot cells cultured in vitro.

    abstract::Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ab...


    pub_type: 杂志文章


    authors: Dong JG,Satoh S,Fujii T

    更新日期:1988-03-01 00:00:00

  • A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation.

    abstract::Plastoglobules are lipid droplets present in all plastid types. In chloroplasts, they are connected to the thylakoid membrane by the outer lipid half-bilayer. The plastoglobule core is composed of neutral lipids most prominently the prenylquinones, triacylglycerols, fatty acid phytyl esters but likely also unknown com...


    pub_type: 杂志文章,评审


    authors: Besagni C,Kessler F

    更新日期:2013-02-01 00:00:00

  • A method for high-frequency intergeneric fusion of plant protoplasts.

    abstract::Protoplasts of Vicia hajastana Grossh. obtained from suspension-culture cells and Pisum sativum L. obtained from leaves adhered tightly to each other in concentrated solutions of high-molecular-weight polyethylene glycol (PEG). The adhesion occurred non-specifically between the free protoplasts from the same species a...


    pub_type: 杂志文章


    authors: Kao KN,Michayluk MR

    更新日期:1974-12-01 00:00:00

  • Brassinolide may control aquaporin activities in Arabidopsis thaliana.

    abstract::It is usually assumed that aquaporins present in the cellular membranes could be an important route in the control of water flux in plants, but evidence for this hypothesis is scarce. In this paper, we report measurements of the osmotic permeability (P(os) of protoplasts isolated from hypocotyls of wild-type and mutan...


    pub_type: 杂志文章


    authors: Morillon R,Catterou M,Sangwan RS,Sangwan BS,Lassalles JP

    更新日期:2001-01-01 00:00:00

  • The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco expiants at a large range of concentrations.

    abstract::Short-term applications of very high concentrations of 1-naphthaleneacetic acid (NAA) to expiants from flower stalks of tobacco (Nicotiana tabacum L. cv. Samsun) induced flower-bud regeneration to the same extent as longer or continuous incubation on lower concentrations. The maximum number of flower buds per explant ...


    pub_type: 杂志文章


    authors: Smulders MJ,Visser EJ,Croes AF,Wullems GJ

    更新日期:1990-02-01 00:00:00

  • Identification of genes differentially expressed in ectomycorrhizal roots during the Pinus pinaster-Laccaria bicolor interaction.

    abstract::Ectomycorrhizal associations are of major ecological importance in temperate and boreal forests. The development of a functional ectomycorrhiza requires many genetic and biochemical changes. In this study, suppressive subtraction hybridization was used to identify differentially expressed genes in the roots of maritim...


    pub_type: 杂志文章


    authors: Flores-Monterroso A,Canales J,de la Torre F,Ávila C,Cánovas FM

    更新日期:2013-06-01 00:00:00

  • Zebra chip disease enhances respiration and oxidative stress of potato tubers (Solanum tuberosum L.).

    abstract:MAIN CONCLUSION:The physiological phenotype of potato tubers afflicted by zebra chip disease is characterized by increased oxidative stress metabolism and upregulation of systems for its mitigation. Starch catabolism and extensive buildup of reducing sugars render potatoes infected with zebra chip (ZC) pathogen (Candid...


    pub_type: 杂志文章


    authors: Kumar GNM,Knowles LO,Knowles NR

    更新日期:2017-10-01 00:00:00

  • The effect of Cl(-) upon the sensitivity of starch-containing and starch-deficient stomata and guard cell protoplasts towards potassium ions, fusicoccin and abscisic acid.

    abstract::Chloride ions are necessary to compensate for the positively charged potassium ions imported into guard cells of Allium cepa L. during stomatal opening. Therefore an external Cl(-) supply of intact Allium plants is important. But high levels of chloride have been found to reduce the sensitivity of the starch-lacking s...


    pub_type: 杂志文章


    authors: Schnabl H

    更新日期:1978-01-01 00:00:00

  • Correlative aging and transport of P(32) in corn leaves under the influence of kinetin.

    abstract::1. The course of senescence in leaves of corn seedlings, as measured by the loss of chlorophyll, is shown to be controlled by two correlative influences: a) In the isolated leaf, the base tends to enhance yellowing of the tip region. b) In the leaf attached to the intact plant, correlative aging is delayed by interact...


    pub_type: 杂志文章


    authors: Müller K,Leopold AC

    更新日期:1966-06-01 00:00:00

  • Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs.

    abstract::Plant defence strategies usually involve the action of several gene products. Transgenic resistance strategies are likely to have enhanced efficacy when they involve more than one transgene. Here we explore possible mechanisms for the co-delivery of multiple effectors via a single transgene. As an example we report th...


    pub_type: 杂志文章


    authors: Urwin PE,McPherson MJ,Atkinson HJ

    更新日期:1998-04-01 00:00:00

  • Inhibition of lycopene cyclase results in accumulation of chlorophyll precursors.

    abstract::Free porphyrins and their magnesium complexes, including chlorophylls, are potent photo-sensitizers. Plants usually accumulate these compounds bound to proteins together with protective compounds like carotenoids. Besides their protective role, carotenoids can play a structural role in these complexes. To analyze the ...


    pub_type: 杂志文章


    authors: La Rocca N,Rascio N,Oster U,Rüdiger W

    更新日期:2007-03-01 00:00:00

  • Transcriptomic variation of the flower-fruit transition in Physalis and Solanum.

    abstract:MAIN CONCLUSION:Gene expression variations in response to fertilization between Physalis and Solanum might play essential roles in species divergence and fruit evolution. Fertilization triggers variation in fruit development and morphology. The Chinese lantern, a morphological novelty derived from the calyx, is formed ...


    pub_type: 杂志文章


    authors: Gao H,Li J,Wang L,Zhang J,He C

    更新日期:2020-07-27 00:00:00

  • Diversity in subcellular targeting of the PP2A B'eta subfamily members.

    abstract::Protein phosphatase 2A (PP2A) is a serine/threonine-specific phosphatase comprising a catalytic subunit (C), a scaffolding subunit (A), and a regulatory subunit (B). The B subunits are believed to be responsible for substrate specificity and localization of the PP2A complex. In plants, three families of B subunits exi...


    pub_type: 杂志文章


    authors: Matre P,Meyer C,Lillo C

    更新日期:2009-10-01 00:00:00

  • Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae).

    abstract:MAIN CONCLUSION:Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell d...


    pub_type: 杂志文章


    authors: Wróbel-Marek J,Kurczyńska E,Płachno BJ,Kozieradzka-Kiszkurno M

    更新日期:2017-03-01 00:00:00

  • Action spectra for chromatic adaptation in the blue-green alga Fremyella diplosiphon.

    abstract::Action spectra for chromatic adaptation in Fremyella diplosiphon Drouet have been determined using techniques previously described. Action maxima are at 540 nm, with a half-band width of 80 nm, for induction of phycoerythrin synthesis (green action) and at 650 nm, with a half-band width of 90 nm, for reversal of induc...


    pub_type: 杂志文章


    authors: Vogelmann TC,Scheibe J

    更新日期:1978-01-01 00:00:00

  • Fast track to the trichome: induction of N-acyl nornicotines precedes nicotine induction in Nicotiana repanda.

    abstract::Nicotiana repanda Wildenow ex Lehmann acylates nornicotine in its trichomes to produce N-acyl-nornicotine (NacNN) alkaloids which are dramatically more toxic than nicotine is to the nicotine-adapted herbivore, Manduca sexta. These NacNNs, like nicotine, were induced by methyl jasmonate (MeJA) and wounding, but the 2-f...


    pub_type: 杂志文章


    authors: Laue G,Preston CA,Baldwin IT

    更新日期:2000-02-01 00:00:00