Abstract：
:A three-step chromatographic procedure was developed for purification of cardenolide 16'-O-glucohydrolase (CGH) from Digitalis lanata Ehrh. leaves, including Phenyl-Sepharose hydrophobic interaction chromatography followed by SP-Sepharose cation exchange and Q-Sepharose anion-exchange chromatography. Starting with acetone dry powder the purification resulted in an 760-fold enrichment of CGH. Molecular weight, substrate specificity, pH optimum and temperature stability of CGH were determined. Antibodies against CGH were prepared in rabbits. The SDS gel electrophoresis of protein extracts from leaves of D. lanata and other D. species showed bands at 70 kDa and 36 kDa reacting with the antibodies. The 70-kDa protein is the main protein stained with CGH antibodies in freshly prepared extracts of D. lanata. It may represent undegraded CGH. The 36-kDa protein is enriched in aged CGH preparations. It is probably a degradation product. Proteins related to 70-kDa and 36-kDa bands also occur in crude protein preparations from leaves of D. heywoodii P. et M. Silva, D. mariana Boiss., D. purpurea L., and D. thapsi L. indicating that CGH is also present in these species. Purified CGH was digested with proteases V8 and Lys-C and the resulting fragments obtained were sequenced. One fragment had the typical amino-acid sequence of the catalytic center of family-1 glycosyl hydrolases (EC 3.2.1.x). Cardenolide 16'-O-glucohydrolase, like the other members of this enzyme family, appeared to have a glutamic acid residue directly involved in glycosidic bond cleavage as a nucleophile.
journal_name
Plantajournal_title
Plantaauthors
Schöniger R,Lindemann P,Grimm R,Eckerskorn C,Luckner Mdoi
10.1007/s004250050346subject
Has Abstractpub_date
1998-07-01 00:00:00pages
477-82issue
3eissn
0032-0935issn
1432-2048pii
10.1007/s004250050346journal_volume
205pub_type
杂志文章相关文献
PLANTA文献大全abstract：:Three cDNA clones encoding lipid transfer proteins (LTPs) were isolated by applying the rapid amplification of cDNA ends (RACE) protocol to imbibed seeds and germinating seedlings Brassica napus. The deduced amino-acid sequences show a great degree of homology and they exhibit the common features shared by all LTPs. T...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00196563
更新日期：1996-01-01 00:00:00
abstract：:Helminthosporol, helminthosporic acid, dihydrohelminthosporic acid, and steviol have been tested for their ability to induce antheridium formation in Anemia phyllitidis. All helminthosporol derivatives posses a limited but real activity. Steviol proved to be inactive.All the substance tested have a significant effect ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388328
更新日期：1967-06-01 00:00:00
abstract：:A novel plant lectin was isolated from salt-stressed rice (Oryza sativa L.) plants and partially characterized. The lectin occurs as a natural mixture of two closely related isoforms consisting of two identical non-covalently linked subunits of 15 kDa. Both isoforms are best inhibited by mannose and exhibit potent mit...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050705
更新日期：2000-05-01 00:00:00
abstract：:Tubular evaginations of the plasmalemma of the scutellar epithelial cells of barley are described. The evaginations are similar to those present at other sites where solute flux occurs for a limited period only and wall development of the transfer-cell form has not occured. Differential uptake of the fluorescent dyes ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00393318
更新日期：1982-12-01 00:00:00
abstract：MAIN CONCLUSION:The analysis of early generations of triticale showed numerous rearrangements of the genome. Complexed transformation included loss of chromosomes, t-heterochromatin content changes and the emergence of retrotransposons in new locations. This study investigated certain aspects of genomic transformations...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-017-2827-0
更新日期：2018-04-01 00:00:00
abstract：:Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00380870
更新日期：1980-07-01 00:00:00
abstract：:Elongating upper and non-elongating lower segments of mung bean hypocotyls (Phaseolus aureus Roxb.) were compared in their ability to incorporate labelled polymers into their cell walls when fed with [(14)C]proline. The non-growing segments incorporated more amino acids into the walls and showed a higher degree of hyd...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00429047
更新日期：1974-09-01 00:00:00
abstract：:Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and cinnamic acid 4-hydroxylase (CA4H, EC 1.14.13.11) undergo concomitant increases in activity after a 2 h lag following disc preparation and illumination. The increases in PAL and CA4H activity can be inhibited by actinomycin-D, cordycepin and cycloheximide. It is demons...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387167
更新日期：1977-01-01 00:00:00
abstract：MAIN CONCLUSION:The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. It is ...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-015-2451-9
更新日期：2016-04-01 00:00:00
abstract：:The microtubular cytoskeleton has been studied during development of the zygote, proembryo and free-nuclear endosperm inA. thaliana using immunofluorescence localization of tubulin in enzymatically isolated material. Abundant micro tubules (MTs) are found throughout proembryogenesis. Microtubules in the coenocytic end...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF01102418
更新日期：1991-06-01 00:00:00
abstract：:Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacumL.) and banana [( Musaspp. cv. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0918-y
更新日期：2003-02-01 00:00:00
abstract：:The cytokinin isomers, 1- and 9-isopentenyladenine, are very active inhibitors of cell division in the protonema of the moss Ceratodon purpureus. They also abolish the N(6)-isopentenyladenine-induced stimulation of cell divisions in this material whereas they are inactive on the induction of gametophore buds in moss p...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384115
更新日期：1972-09-01 00:00:00
abstract：:Rust fungi are obligate biotrophic pathogens that differentiate a series of specialized cells to establish infection. One of these cells, the haustorium, which serves to absorb nutrients from living host cells, normally develops only in planta. Here, we show that the rust fungus Uromyces fabae (Pers.) Schroet. stimula...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0320-2
更新日期：2006-11-01 00:00:00
abstract：:The scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of Valencia orange. Separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. The most noticeable differences are the lessened amount of tearing in pr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390215
更新日期：1971-12-01 00:00:00
abstract：:Amaranthus blitoides S. Watson (prostrate pigweed) populations resistant to acetolactate synthase (ALS; EC 4.1.3.18)-inhibiting herbicides and triazines (SuR/TR) were found in Israel. The Ganot population was 6- to 790-fold more resistant to ALS inhibitors than the wild type due to an altered target site. Molecular an...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0955-6
更新日期：2003-04-01 00:00:00
abstract：:Conditions of soil drying and plant growth that lead to non-hydraulic inhibition of leaf elongation and stomatal conductance in maize (Zea mays L.) were investigated using plants grown with their root systems divided between two containers. The soil in one container was allowed to dry while the other container was kep...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00397586
更新日期：1989-11-01 00:00:00
abstract：:To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, ea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1281-y
更新日期：2004-09-01 00:00:00
abstract：:The cuticle is a complex structure of soluble lipids, lipid polymers and polysaccharides. In addition to its functions to reduce water loss and provide a protective barrier, its mechanical properties may be significant to plant growth and development. We investigated the cuticle of Cirsium horridulum Michx. because of...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250100576
更新日期：2001-10-01 00:00:00
abstract：:When (3)H-(14)C-acetate was supplied to Chlorella pyrenoidosa in the light, glycollic acid became rapidly labelled with tritium and (14)C. The [Formula: see text]ratio of glycollate was 10, whilst the ratio was 4 in the acetate added. Both (3)H and (14)C from acetate were present in glycollate before they were present...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386326
更新日期：1967-09-01 00:00:00
abstract：MAIN CONCLUSION:Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-2879-9
更新日期：2018-06-01 00:00:00
abstract：:For phototropic curvature of a green sunflower seedling, only the hypocotyl has to be illuminated; the tip and cotyledons are not involved in stimulus perception. The etiolated seedling is phototropically insensitive, illumination of only the hypocotyl renders it sensitive. It is concluded that the photoreceptor is lo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00393292
更新日期：1981-04-01 00:00:00
abstract：:It is shown that simple, unbranched, plasmodesmata between young xylem ray cells of willow have no direct intercellular continuity apart from the plasmalemma which limits the cytoplasm and lines the plasmodesmatal canal. Each plasmodesma is traversed by a 200 Å diameter tubule (the desmotubule) which has a wall with p...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00398199
更新日期：1968-09-01 00:00:00
abstract：:The control by phytochrome of hypocotyl elongation of light-grown Cucumis sativus L. after a white-light period was examined. The farred-absorbing form of phytochrome inhibits hypocotyl elongation. The response to phytochrome photostationary state (ϕ) is not linear; all values of ϕ from 0.004 to 0.13 promote growth ma...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00396091
更新日期：1985-05-01 00:00:00
abstract：:The qualitative and quantitative composition of the carotenoids of young cells of Euglena gracilis strain 1224-5/9 corresponded to that of the "Z"-strain.The frequently observed yellow-reddish discolouration of ageing cells was found to be caused by a heavy breakdown of the chlorophylls and not by an increased synthes...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387621
更新日期：1971-06-01 00:00:00
abstract：:The effect of chemical stress on the polypeptide composition of the intercellular fluid of barley (Hordeum vulgare L.) and tomato (Lycopersicon esculentum Mill.) leaves has been studied. In some dicotyledonous plant species, including tomato, exposure to chemical stress leads to the denovo synthesis of intercellular p...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392527
更新日期：1989-05-01 00:00:00
abstract：:Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Ra...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-008-0699-z
更新日期：2008-05-01 00:00:00
abstract：:Arabinogalactan proteins (AGPs) are abundant plant cell surface proteoglycans widely distributed in plant species. Since high concentrations of beta-glucosyl Yariv reagent (betaglcY), which binds selectively to AGPs, inhibited cell division of protoplast-regenerated cells of the liverwort Marchantia polymorpha L. (Shi...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-0954-y
更新日期：2009-08-01 00:00:00
abstract：:The salt glands of Aegiceras corniculatum have been examined by light- and electron-microscope techniques. A gland consists of a large number of abutting secretory cells and a single, large basal cell. The secretory cells and basal cell are joined by well defined plasmodesmata. The glandular cuticle shows differences ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386836
更新日期：1971-09-01 00:00:00
abstract：:Acid phosphatase has been localized by cytochemical techniques in aleurone layers of dry barley (Hordeum vulgare L.) grains, in imbibed half-grains and in isolated layers treated with and without gibberellic acid (GA3). A major fraction of the enzyme activity is located in the cell walls. During imbibition and incubat...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388273
更新日期：1974-01-01 00:00:00
abstract：MAIN CONCLUSION:Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-021-03570-y
更新日期：2021-01-18 00:00:00
