Abstract:
:Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC 3.2.1.78) participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase from the endosperm of germinated tomato (Lycopersicon esculentum Mill.) seeds has been isolated and characterized. The amino acid sequence deduced from the 5'-region of the cDNA exactly matches the sequence of the 65 NH2-terminal amino acids determined directly from the purified enzyme. The mature enzyme consists of 346 amino acid residues, it has a calculated M(r) of 38,950 and an isoelectric point of 5.3. Overall, the enzyme exhibits only 28-30% sequence identity with fungal (1-->4)-beta-mannanases, but more highly conserved regions, which may represent catalytic and substrate-binding domains, can be identified. Based on classification of the tomato (1-->4)-beta-mannanase as a member of the family 5 group of glycosyl hydrolases, Glu-148 and Glu-265 would be expected to be the catalytic acid and the catalytic nucleophile, respectively. Southern hybridization analyses indicate that the enzyme is derived from a family of about four genes. Expression of the genes, as determined by the presence of mRNA transcripts in Northern hybridization analyses, occurs in the endosperm of germinated seeds; no transcripts are detected in hypocotyls, cotyledons, roots or leaves.
journal_name
Plantajournal_title
Plantaauthors
Bewley JD,Burton RA,Morohashi Y,Fincher GBdoi
10.1007/s004250050214subject
Has Abstractpub_date
1997-12-01 00:00:00pages
454-9issue
4eissn
0032-0935issn
1432-2048journal_volume
203pub_type
杂志文章相关文献
PLANTA文献大全abstract::Plants have efficient water-transporting vascular networks with a self-recovery function from embolism, which causes fatal discontinuity in sap flow. However, the embolism-refilling process in xylem vessel is still unclear. The water-refilling processes in the individual xylem vessels of excised Arabidopsis roots were...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1889-x
更新日期:2013-08-01 00:00:00
abstract::The cuticle is a complex structure of soluble lipids, lipid polymers and polysaccharides. In addition to its functions to reduce water loss and provide a protective barrier, its mechanical properties may be significant to plant growth and development. We investigated the cuticle of Cirsium horridulum Michx. because of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100576
更新日期:2001-10-01 00:00:00
abstract:MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03435-w
更新日期:2020-08-01 00:00:00
abstract::Sexual interaction between gametes of opposite mating type (mt) of the unicellular green alga Chlamydomonas eugametos starts with agglutination of the cells via particular glycoproteins on the flagellar surface. Purification of these socalled agglutinins was achieved by a three-step procedure consisting of, successive...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395022
更新日期:1987-03-01 00:00:00
abstract::Here we report on the isolation and characterization of a somatic embryogenesis receptor-like kinase (OsSERK1) gene in rice (Oryza sativa). The OsSERK1 gene belongs to a small subfamily of receptor-like kinase genes in rice and shares a highly conserved gene structure and extensive sequence homology with previously re...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-1534-4
更新日期:2005-09-01 00:00:00
abstract::Aseptically cultured lateral buds of Salix viminalis L. collected from field-grown trees exhibited a clear periodicity in their ability to respond to exogenous abscisic acid (ABA). Buds were kept unopened by ABA only when the plants were dormant or entering dormancy. Short days alone did not induce bud dormancy in pot...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392273
更新日期:1986-09-01 00:00:00
abstract::Cotton fibers are single elongated cells that develop from epidermal cells of the ovule. The chronology of fiber differentiation was investigated using cultured ovules. Epidermal cells differentiate into fiber cells approx. 3 d before anthesis. When ovules were cultured on a defined medium, fiber growth could be initi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392435
更新日期:1988-08-01 00:00:00
abstract:MAIN CONCLUSION:Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-021-03570-y
更新日期:2021-01-18 00:00:00
abstract::Leaves ofNerium oleander L. plants, which had been previously kept in a shaded glasshouse for at least two months, were fed 1 mM dithiothreitol (DTT) through their petioles, either for 12h in darkness (overnight) or for 2h in low light (28 μmol photons·m(-2)·s(-1)), in each case followed by a 3-h exposure to high ligh...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF02411406
更新日期:1989-12-01 00:00:00
abstract::De-etiolation involves a number of phenotypic changes as the plants shift from a dark-grown (etiolated) to a light-grown (de-etiolated) morphology. Whilst these light-induced, morphological changes are thought to be mediated by plant hormones, the precise mechanism/s are not yet fully understood. Here we provide furth...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0685-x
更新日期:2008-04-01 00:00:00
abstract:MAIN CONCLUSION:The aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsRNA with sequence identity to the nematode gene in tobacco roots under the influence of strong Arabidopsis ubiquitin (UBQ1) promoter. Root-knot nematodes (RKN...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2211-2
更新日期:2015-03-01 00:00:00
abstract::A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1442-z
更新日期:2005-07-01 00:00:00
abstract::Chloroplasts are the endosymbiotic descendants of cyanobacterium-like prokaryotes. Present genomes of plant and green algae chloroplasts (plastomes) contain ~100 genes mainly encoding for their transcription-/translation-machinery, subunits of the thylakoid membrane complexes (photosystems II and I, cytochrome b ...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-012-1752-5
更新日期:2013-02-01 00:00:00
abstract::A study has been made of the RNA and protein synthesising systems of wheat embryos isolated from seed lots having high viability but differing in vigour. The rate of RNA and protein synthesis in wheat embryos during the early hours of germination is related to the vigour of the seed lot. The imposition of a stress fac...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385609
更新日期:1980-11-01 00:00:00
abstract::Incorporation of [(14)C]uridine into ribosomal RNA and ribosomal RNA precursor fractions was studied in isolated wheat embryos and in embryos remaining within the intact grains during germination. In isolated embryos, radioactive 25S and 18S as well as 31S RNA species appeared already within the first 3 h of germinati...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384191
更新日期:1977-01-01 00:00:00
abstract::The new type blue light (BL) receptor aureochrome (AUREO) was recently discovered in a stramenopile alga, Vaucheria (Takahashi et al. Proc Natl Acad Sci USA 104(49):19625-19630, 2007). AUREO has a bZIP (basic region/leucine zipper) and BL-sensing light-oxygen-voltage (LOV) domain and functions as a BL-activated transc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0967-6
更新日期:2009-08-01 00:00:00
abstract::The dynamics of actin-filament organization in pollen-tube subprotoplasts ofNicotiana tabacum L. cv. Samsun during regeneration and outgrowth was examined using phalloidin probes and a non-fixation method. A succession of actin arrays was examined during subprotoplast regeneration that strongly resembled the actin dyn...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF02411443
更新日期:1990-03-01 00:00:00
abstract::Glycosylation of sterols, catalysed by sterol glycosyltransferases (SGTs), improves the sterol solubility, chemical stability and compartmentalization, and helps plants to adapt to environmental changes. The SGTs in medicinal plants are of particular interest for their role in the biosynthesis of pharmacologically act...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2046-x
更新日期:2014-06-01 00:00:00
abstract::The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1264-z
更新日期:2004-08-01 00:00:00
abstract::Temperature and light are important environmental factors that affect flavonoid biosynthesis in grape berry skin. However, the interrelationships between temperature and light effects on flavonoid biosynthesis have not been fully elucidated at the molecular level. Here, we investigated the effects of temperature and l...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1650-x
更新日期:2012-10-01 00:00:00
abstract::Freshly isolated protoplasts or separated cells prepared from leaves of Nicotiana tabacum L. cvs. Xanthincor Turkish Samsun exhibit substantially greater incorporation of [(3)H] leucine and [(3)H] uridine into protoplast protein and RNA, respectively, when dilute rather than concentrated suspensions are incubated. Thi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387350
更新日期:1976-01-01 00:00:00
abstract::In the epidermal cells of onion (Allium cepa L.) bulb scales the endoplasmic reticulum (ER) can be subdivided into three domains: a peripheral tubular network, cisternae, and long tubular strands. The latter are the form in which the ER is moved in onion cells. During cold treatment the arrangement of the three domain...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392816
更新日期:1989-02-01 00:00:00
abstract::In order to elucidate the involvement of brassinosteroids in the cell elongation process leading to normal plant morphology, indirect immunofluorescence and molecular techniques were use to study the expression of tubulin genes in the bul1-1 dwarf mutant of Arabidopsis thaliana (L.) Heynh., the characteristics of whic...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000467
更新日期:2001-04-01 00:00:00
abstract::Physiological data from extreme habitat organisms during stresses are vital information for comprehending their survival. The intertidal seaweeds are exposed to a combination of environmental stresses, the most influential one being regular dehydration and re-hydration. Porphyra katadai var. hemiphylla is a unique int...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0874-2
更新日期:2009-03-01 00:00:00
abstract::Chlamydomonas reinhardtii Dangeard generates photoreceptor currents (PCs) upon light excitation. These currents play a key role in the signal transduction chain for photomotility responses. We have previously found that inhibition of PCs by tryptone occurs only in gametes that display chemotaxis toward this agent, and...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0357-2
更新日期:2007-01-01 00:00:00
abstract::Through the use of gas chromatography-mass spectrometry techniques two simple brominated phenols, 2,3-dibromo-4,5-dihydroxybenzyl alcohol (lanosol) and 3,5-dibromo-p-hydroxybenzyl alcohol, were identified in an axenically cultured bluegreen alga, Calothrix brevissima. The alga was grown in a mineral medium supplemente...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388608
更新日期:1973-03-01 00:00:00
abstract::The occurrence of heterotrophic nitrification in nitrogen-starved cells of Ankistrodesmus braunii was confirmed. The levels of nitrate and nitrite were measured over a period of four weeks. The validity of quantitative determinations in the presence of highly active nitrate and nitrite reductases is discussed. Whereas...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390025
更新日期:1976-01-01 00:00:00
abstract::Particulate enzymic preparations obtained from homogenates of differentiated xylem cells isolated from sycamore trees, catalyzed the formation of a radioactive xylan in the presence of UDP-D-[U-(14)C]xylose as substrate. The synthesized xylan was not dialyzable through Visking cellophane tubing. Successive extraction ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384237
更新日期:1981-01-01 00:00:00
abstract:MAIN CONCLUSION:A mixture of resins based on aliphatic esters and carboxylic acids occurs in distantly related genera Peperomia and Roridula , serving different functions as adhesion in seed dispersal and prey capture. According to mechanical characteristics, adhesive secretions on both leaves of the carnivorous flypap...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2496-4
更新日期:2016-07-01 00:00:00
abstract::Mineral nutrients are distributed in a non-uniform manner in the soil. Plasticity in root responses to the availability of mineral nutrients is believed to be important for optimizing nutrient acquisition. The response of root architecture to heterogeneous nutrient availability has been documented in various plant spe...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-014-2150-y
更新日期:2014-10-01 00:00:00