Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum).

Abstract:

:Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC 3.2.1.78) participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase from the endosperm of germinated tomato (Lycopersicon esculentum Mill.) seeds has been isolated and characterized. The amino acid sequence deduced from the 5'-region of the cDNA exactly matches the sequence of the 65 NH2-terminal amino acids determined directly from the purified enzyme. The mature enzyme consists of 346 amino acid residues, it has a calculated M(r) of 38,950 and an isoelectric point of 5.3. Overall, the enzyme exhibits only 28-30% sequence identity with fungal (1-->4)-beta-mannanases, but more highly conserved regions, which may represent catalytic and substrate-binding domains, can be identified. Based on classification of the tomato (1-->4)-beta-mannanase as a member of the family 5 group of glycosyl hydrolases, Glu-148 and Glu-265 would be expected to be the catalytic acid and the catalytic nucleophile, respectively. Southern hybridization analyses indicate that the enzyme is derived from a family of about four genes. Expression of the genes, as determined by the presence of mRNA transcripts in Northern hybridization analyses, occurs in the endosperm of germinated seeds; no transcripts are detected in hypocotyls, cotyledons, roots or leaves.

journal_name

Planta

journal_title

Planta

authors

Bewley JD,Burton RA,Morohashi Y,Fincher GB

doi

10.1007/s004250050214

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

454-9

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

203

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Hydraulic characteristics of water-refilling process in excised roots of Arabidopsis.

    abstract::Plants have efficient water-transporting vascular networks with a self-recovery function from embolism, which causes fatal discontinuity in sap flow. However, the embolism-refilling process in xylem vessel is still unclear. The water-refilling processes in the individual xylem vessels of excised Arabidopsis roots were...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1889-x

    authors: Lee SJ,Hwang BG,Kim HK

    更新日期:2013-08-01 00:00:00

  • Biochemical analysis of elastic and rigid cuticles of Cirsium horridulum.

    abstract::The cuticle is a complex structure of soluble lipids, lipid polymers and polysaccharides. In addition to its functions to reduce water loss and provide a protective barrier, its mechanical properties may be significant to plant growth and development. We investigated the cuticle of Cirsium horridulum Michx. because of...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100576

    authors: Marga F,Pesacreta TC,Hasenstein KH

    更新日期:2001-10-01 00:00:00

  • Comprehensive analysis of polygalacturonase gene family highlights candidate genes related to pollen development and male fertility in wheat (Triticum aestivum L.).

    abstract:MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03435-w

    authors: Ye J,Yang X,Yang Z,Niu F,Chen Y,Zhang L,Song X

    更新日期:2020-08-01 00:00:00

  • Composition and properties of the sexual agglutinins of the flagellated green alga Chlamydomonas eugametos.

    abstract::Sexual interaction between gametes of opposite mating type (mt) of the unicellular green alga Chlamydomonas eugametos starts with agglutination of the cells via particular glycoproteins on the flagellar surface. Purification of these socalled agglutinins was achieved by a three-step procedure consisting of, successive...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00395022

    authors: Samson MR,Klis FM,Homan WL,van Egmond P,Musgrave A,van den Ende H

    更新日期:1987-03-01 00:00:00

  • Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection.

    abstract::Here we report on the isolation and characterization of a somatic embryogenesis receptor-like kinase (OsSERK1) gene in rice (Oryza sativa). The OsSERK1 gene belongs to a small subfamily of receptor-like kinase genes in rice and shares a highly conserved gene structure and extensive sequence homology with previously re...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-005-1534-4

    authors: Hu H,Xiong L,Yang Y

    更新日期:2005-09-01 00:00:00

  • Periodicity of response to abscisic acid in lateral buds of willow (Salix viminalis L.).

    abstract::Aseptically cultured lateral buds of Salix viminalis L. collected from field-grown trees exhibited a clear periodicity in their ability to respond to exogenous abscisic acid (ABA). Buds were kept unopened by ABA only when the plants were dormant or entering dormancy. Short days alone did not induce bud dormancy in pot...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392273

    authors: Barros RS,Neill SJ

    更新日期:1986-09-01 00:00:00

  • Chronology of the differentiation of cotton (Gossypium hirsutum L.) fiber cells.

    abstract::Cotton fibers are single elongated cells that develop from epidermal cells of the ovule. The chronology of fiber differentiation was investigated using cultured ovules. Epidermal cells differentiate into fiber cells approx. 3 d before anthesis. When ovules were cultured on a defined medium, fiber growth could be initi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392435

    authors: Graves DA,Stewart JM

    更新日期:1988-08-01 00:00:00

  • Linking jasmonates with vitamin E accumulation in plants: a case study in the Mediterranean shrub Cistus albidus L.

    abstract:MAIN CONCLUSION:Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-021-03570-y

    authors: Casadesús A,Bouchikh R,Pérez-Llorca M,Munné-Bosch S

    更新日期:2021-01-18 00:00:00

  • Dithiothreitol, an inhibitor of violaxanthin de-epoxidation, increases the susceptibility of leaves ofNerium oleander L. to photoinhibition of photosynthesis.

    abstract::Leaves ofNerium oleander L. plants, which had been previously kept in a shaded glasshouse for at least two months, were fed 1 mM dithiothreitol (DTT) through their petioles, either for 12h in darkness (overnight) or for 2h in low light (28 μmol photons·m(-2)·s(-1)), in each case followed by a 3-h exposure to high ligh...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02411406

    authors: Winter K,Königer M

    更新日期:1989-12-01 00:00:00

  • The hormonal regulation of de-etiolation.

    abstract::De-etiolation involves a number of phenotypic changes as the plants shift from a dark-grown (etiolated) to a light-grown (de-etiolated) morphology. Whilst these light-induced, morphological changes are thought to be mediated by plant hormones, the precise mechanism/s are not yet fully understood. Here we provide furth...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-007-0685-x

    authors: Symons GM,Smith JJ,Nomura T,Davies NW,Yokota T,Reid JB

    更新日期:2008-04-01 00:00:00

  • Disruption of prefoldin-2 protein synthesis in root-knot nematodes via host-mediated gene silencing efficiently reduces nematode numbers and thus protects plants.

    abstract:MAIN CONCLUSION:The aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsRNA with sequence identity to the nematode gene in tobacco roots under the influence of strong Arabidopsis ubiquitin (UBQ1) promoter. Root-knot nematodes (RKN...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2211-2

    authors: Ajjappala H,Chung HY,Sim JS,Choi I,Hahn BS

    更新日期:2015-03-01 00:00:00

  • LeRALF, a plant peptide that regulates root growth and development, specifically binds to 25 and 120 kDa cell surface membrane proteins of Lycopersicon peruvianum.

    abstract::A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1442-z

    authors: Scheer JM,Pearce G,Ryan CA

    更新日期:2005-07-01 00:00:00

  • How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.

    abstract::Chloroplasts are the endosymbiotic descendants of cyanobacterium-like prokaryotes. Present genomes of plant and green algae chloroplasts (plastomes) contain ~100 genes mainly encoding for their transcription-/translation-machinery, subunits of the thylakoid membrane complexes (photosystems II and I, cytochrome b ...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-012-1752-5

    authors: Lyska D,Meierhoff K,Westhoff P

    更新日期:2013-02-01 00:00:00

  • Nucleic acid and protein synthesis and loss of vigour in germinating wheat embryos.

    abstract::A study has been made of the RNA and protein synthesising systems of wheat embryos isolated from seed lots having high viability but differing in vigour. The rate of RNA and protein synthesis in wheat embryos during the early hours of germination is related to the vigour of the seed lot. The imposition of a stress fac...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385609

    authors: Blowers LE,Stormonth DA,Bray CM

    更新日期:1980-11-01 00:00:00

  • A comparison of the activation of ribosomal RNA synthesis during germination of isolated and non-isolated embryos of Triticum aestivum L.

    abstract::Incorporation of [(14)C]uridine into ribosomal RNA and ribosomal RNA precursor fractions was studied in isolated wheat embryos and in embryos remaining within the intact grains during germination. In isolated embryos, radioactive 25S and 18S as well as 31S RNA species appeared already within the first 3 h of germinati...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384191

    authors: Grzelczak Z,Buchowicz J

    更新日期:1977-01-01 00:00:00

  • Distribution and phylogeny of the blue light receptors aureochromes in eukaryotes.

    abstract::The new type blue light (BL) receptor aureochrome (AUREO) was recently discovered in a stramenopile alga, Vaucheria (Takahashi et al. Proc Natl Acad Sci USA 104(49):19625-19630, 2007). AUREO has a bZIP (basic region/leucine zipper) and BL-sensing light-oxygen-voltage (LOV) domain and functions as a BL-activated transc...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0967-6

    authors: Ishikawa M,Takahashi F,Nozaki H,Nagasato C,Motomura T,Kataoka H

    更新日期:2009-08-01 00:00:00

  • Organization of actin filaments in regenerating and outgrowing subprotoplasts from pollen tubes ofNicotiana tabacum L.

    abstract::The dynamics of actin-filament organization in pollen-tube subprotoplasts ofNicotiana tabacum L. cv. Samsun during regeneration and outgrowth was examined using phalloidin probes and a non-fixation method. A succession of actin arrays was examined during subprotoplast regeneration that strongly resembled the actin dyn...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02411443

    authors: Rutten TL,Derksen J

    更新日期:1990-03-01 00:00:00

  • WsSGTL1 gene from Withania somnifera, modulates glycosylation profile, antioxidant system and confers biotic and salt stress tolerance in transgenic tobacco.

    abstract::Glycosylation of sterols, catalysed by sterol glycosyltransferases (SGTs), improves the sterol solubility, chemical stability and compartmentalization, and helps plants to adapt to environmental changes. The SGTs in medicinal plants are of particular interest for their role in the biosynthesis of pharmacologically act...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2046-x

    authors: Pandey V,Niranjan A,Atri N,Chandrashekhar K,Mishra MK,Trivedi PK,Misra P

    更新日期:2014-06-01 00:00:00

  • The novel gene CpEdi-9 from the resurrection plant C. plantagineum encodes a hydrophilic protein and is expressed in mature seeds as well as in response to dehydration in leaf phloem tissues.

    abstract::The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1264-z

    authors: Rodrigo MJ,Bockel C,Blervacq AS,Bartels D

    更新日期:2004-08-01 00:00:00

  • Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions.

    abstract::Temperature and light are important environmental factors that affect flavonoid biosynthesis in grape berry skin. However, the interrelationships between temperature and light effects on flavonoid biosynthesis have not been fully elucidated at the molecular level. Here, we investigated the effects of temperature and l...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1650-x

    authors: Azuma A,Yakushiji H,Koshita Y,Kobayashi S

    更新日期:2012-10-01 00:00:00

  • Cell concentration as a factor in precursor incorporation by tobacco leaf protoplasts or separated cells.

    abstract::Freshly isolated protoplasts or separated cells prepared from leaves of Nicotiana tabacum L. cvs. Xanthincor Turkish Samsun exhibit substantially greater incorporation of [(3)H] leucine and [(3)H] uridine into protoplast protein and RNA, respectively, when dilute rather than concentrated suspensions are incubated. Thi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387350

    authors: Rubin G,Zaitlin M

    更新日期:1976-01-01 00:00:00

  • Reorganization of the endoplasmic reticulum in epidermal cells of onion bulb scales after cold stress: Involvement of cytoskeletal elements.

    abstract::In the epidermal cells of onion (Allium cepa L.) bulb scales the endoplasmic reticulum (ER) can be subdivided into three domains: a peripheral tubular network, cisternae, and long tubular strands. The latter are the form in which the ER is moved in onion cells. During cold treatment the arrangement of the three domain...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392816

    authors: Quader H,Hofmann A,Schnepf E

    更新日期:1989-02-01 00:00:00

  • Brassinosteroids, microtubules and cell elongation in Arabidopsis thaliana. II. Effects of brassinosteroids on microtubules and cell elongation in the bul1 mutant.

    abstract::In order to elucidate the involvement of brassinosteroids in the cell elongation process leading to normal plant morphology, indirect immunofluorescence and molecular techniques were use to study the expression of tubulin genes in the bul1-1 dwarf mutant of Arabidopsis thaliana (L.) Heynh., the characteristics of whic...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000467

    authors: Catterou M,Dubois F,Schaller H,Aubanelle L,Vilcot B,Sangwan-Norreel BS,Sangwan RS

    更新日期:2001-04-01 00:00:00

  • Photosynthetic parameters of sexually different parts of Porphyra katadai var. hemiphylla (Bangiales, Rhodophyta) during dehydration and re-hydration.

    abstract::Physiological data from extreme habitat organisms during stresses are vital information for comprehending their survival. The intertidal seaweeds are exposed to a combination of environmental stresses, the most influential one being regular dehydration and re-hydration. Porphyra katadai var. hemiphylla is a unique int...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-008-0874-2

    authors: Lin AP,Wang GC,Yang F,Pan GH

    更新日期:2009-03-01 00:00:00

  • Changes in photoreceptor currents and their sensitivity to the chemoeffector tryptone during gamete mating in Chlamydomonas reinhardtii.

    abstract::Chlamydomonas reinhardtii Dangeard generates photoreceptor currents (PCs) upon light excitation. These currents play a key role in the signal transduction chain for photomotility responses. We have previously found that inhibition of PCs by tryptone occurs only in gametes that display chemotaxis toward this agent, and...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0357-2

    authors: Govorunova EG,Voytsekh OO,Sineshchekov OA

    更新日期:2007-01-01 00:00:00

  • Simple brominated phenols in the bluegreen alga Calothrix brevissima West.

    abstract::Through the use of gas chromatography-mass spectrometry techniques two simple brominated phenols, 2,3-dibromo-4,5-dihydroxybenzyl alcohol (lanosol) and 3,5-dibromo-p-hydroxybenzyl alcohol, were identified in an axenically cultured bluegreen alga, Calothrix brevissima. The alga was grown in a mineral medium supplemente...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388608

    authors: Pedersén M,Dasilva EJ

    更新日期:1973-03-01 00:00:00

  • Intracellular appearance of nitrite and nitrate in nitrogen-starved cells of Ankistrodesmus braunii.

    abstract::The occurrence of heterotrophic nitrification in nitrogen-starved cells of Ankistrodesmus braunii was confirmed. The levels of nitrate and nitrite were measured over a period of four weeks. The validity of quantitative determinations in the presence of highly active nitrate and nitrite reductases is discussed. Whereas...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390025

    authors: Spiller H,Dietsch E,Kessler E

    更新日期:1976-01-01 00:00:00

  • Xylan synthetase activity in differentiated xylem cells of sycamore trees (Acer pseudoplatanus).

    abstract::Particulate enzymic preparations obtained from homogenates of differentiated xylem cells isolated from sycamore trees, catalyzed the formation of a radioactive xylan in the presence of UDP-D-[U-(14)C]xylose as substrate. The synthesized xylan was not dialyzable through Visking cellophane tubing. Successive extraction ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384237

    authors: Dalessandro G,Northcote DH

    更新日期:1981-01-01 00:00:00

  • Plant pressure sensitive adhesives: similar chemical properties in distantly related plant lineages.

    abstract:MAIN CONCLUSION:A mixture of resins based on aliphatic esters and carboxylic acids occurs in distantly related genera Peperomia and Roridula , serving different functions as adhesion in seed dispersal and prey capture. According to mechanical characteristics, adhesive secretions on both leaves of the carnivorous flypap...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-016-2496-4

    authors: Frenzke L,Lederer A,Malanin M,Eichhorn KJ,Neinhuis C,Voigt D

    更新日期:2016-07-01 00:00:00

  • Phenotypic plasticity of the maize root system in response to heterogeneous nitrogen availability.

    abstract::Mineral nutrients are distributed in a non-uniform manner in the soil. Plasticity in root responses to the availability of mineral nutrients is believed to be important for optimizing nutrient acquisition. The response of root architecture to heterogeneous nutrient availability has been documented in various plant spe...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-014-2150-y

    authors: Yu P,White PJ,Hochholdinger F,Li C

    更新日期:2014-10-01 00:00:00