Abstract:
MAIN CONCLUSION:The aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsRNA with sequence identity to the nematode gene in tobacco roots under the influence of strong Arabidopsis ubiquitin (UBQ1) promoter. Root-knot nematodes (RKNs) are sedentary endoparasites infecting a wide range of plant species. They parasitise the root system, thereby disrupting water and nutrient uptake and causing major reductions in crop yields. The most reliable means of controlling RKNs is via the use of soil fumigants such as methyl bromide. With the emergence of RNA interference (RNAi) technology, which permits host-mediated nematode gene silencing, a new strategy to control plant pathogens has become available. In the present study, we investigated host-induced RNAi gene silencing of prefoldin-2 in transgenic Nicotiana benthamiana. Reductions in prefoldin-2 mRNA transcript levels were observed when nematodes were soaked in a dsRNA solution in vitro. Furthermore, nematode reproduction was suppressed in RNAi transgenic lines, as evident by reductions in the numbers of root knots (by 34-60 % in independent RNAi lines) and egg masses (by 33-58 %). Endogenous expression of prefoldin-2, analysed via real-time polymerase chain reaction and Western blotting, revealed that the gene was strongly expressed in the pre-parasitic J2 stage. Our observations demonstrate the relevance and potential importance of targeting the prefoldin gene during the nematode life cycle. The work also suggests that further improvements in silencing efficiency in economically important crops can be accomplished using RNAi directed against plant-parasitic nematodes.
journal_name
Plantajournal_title
Plantaauthors
Ajjappala H,Chung HY,Sim JS,Choi I,Hahn BSdoi
10.1007/s00425-014-2211-2subject
Has Abstractpub_date
2015-03-01 00:00:00pages
773-87issue
3eissn
0032-0935issn
1432-2048journal_volume
241pub_type
杂志文章相关文献
PLANTA文献大全abstract::The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1264-z
更新日期:2004-08-01 00:00:00
abstract::Microautoradiography was used to follow the translocation pathways of (14)C-labeled photosynthate from mature source leaves, through the stem, to immature sink leaves three nodes above. Translocation occurred in specific bundles of the midveins and petioles of both the source and sink leaves and in the interjacent int...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397473
更新日期:1982-12-01 00:00:00
abstract:MAIN CONCLUSION:The interaction between enzymatic and non-enzymatic antioxidants, endogenous levels of ABA and ABA-GE, the rapid recuperation of photosynthetic proteins under re-watering as well the high level of antioxidant proteins in previously drought-stressed plants under re-watering conditions, will contribute to...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2221-0
更新日期:2015-04-01 00:00:00
abstract::In order to survive sunlight in the absence of water, desiccation-tolerant green plants need to be protected against photooxidation. During drying of the chlorolichen Cladonia rangiformis and the cyanolichen Peltigera neckeri, chlorophyll fluorescence decreased and stable light-dependent charge separation in reaction ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0766-5
更新日期:2008-09-01 00:00:00
abstract::The purpose of these studies was to find which steps in the biosynthetic pathway of carotenoids in Fusarium are under photoregulation. After separation by column chromatography ζ-carotene, neurosporene, γ-carotene, torulene, neurosporaxanthin and lycopene were identified from their absorption spectra in visible light ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387760
更新日期:1970-06-01 00:00:00
abstract::During a search for genes with induced or enhanced expression in the early stages of development of stem-borne nodules on Sesbania rostrata, a cDNA with homology to chalcone reductase (CHR) genes was isolated. Here, we describe the characterization of a full-length CHR cDNA (Srchr1) and the pattern of CHR transcript a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050605
更新日期:1999-07-01 00:00:00
abstract::Phototropic stimulation induces a spatial memory. This was inferred from experiments with maize (Zea mays L.) coleoptiles involving opposing blue-light pulses, separated by variable time intervals, and rotation on a horizontal clinostat (Nick and Schafer, 1988b, Planta 175, 380-388). In those experiments, individual s...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201066
更新日期:1991-10-01 00:00:00
abstract:MAIN CONCLUSIONS:One hundred DDB1 (damaged DNA binding protein-1)-binding WD40-repeat domain (DWD) family genes were identified in the S. lycopersicum genome. The DWD genes encode proteins presumably functioning as the substrate recognition subunits of the cullin4-ring ubiquitin E3 ligase complex. These findings provid...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2258-8
更新日期:2015-06-01 00:00:00
abstract::In a previous paper (HOCK, KüHNERT and MOHR, 1965) we have shown that fat degradation in the cotyledons of the mustard seedling (Sinapis alba L). is under the control of active phytochrome (=P730), at least to a certain extent. One could imagine that P730 might exert this control over fat consumption through isocitrit...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387481
更新日期:1966-06-01 00:00:00
abstract:MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2431-0
更新日期:2016-03-01 00:00:00
abstract::Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC 3.2.1.78) participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase fr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050214
更新日期:1997-12-01 00:00:00
abstract::Wild-type (Ph1) and adenine-requiring (Ad1) cell lines of Datura innoxia Mill. were used in experiments to evaluate arsenate as a growth-lethal compound and its use as a counterselection agent. These experiments led to the devising of methods for the recovery of Ph1 and Ad1 cells after arsenate treatment when plated a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392866
更新日期:1984-02-01 00:00:00
abstract::In order to analyze the genetic programme expressed during the early stages of embryogenesis a cDNA clone bank was constructed from desiccation-tolerant excised barley embryos 18 d after pollination (D. Bartels et al., 1988, Planta 175, 485-492). One of the selected cDNA clones pG31 encodes a transcript of 1300 nucleo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00203590
更新日期:1994-01-01 00:00:00
abstract::When plant storage tissue is cut into thin disks it rapidly develops a high capacity to absorb phosphate from dilute solutions. The recent trend has been to attribute the development of this ability to the increased metabolic activity of bacteria known to be containing the tissue. Data presented in this paper shows th...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387651
更新日期:1970-03-01 00:00:00
abstract::Pea plants grown under different conditions of cultivation, and eight different plant species with variegated leaves were used to study the intracellular localization of shikimate oxidoreductase (EC 1.1.1.25), the marker enzyme of the pre-chorismate pathway. The two series of experiments indicated an intra-and an extr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397408
更新日期:1983-07-01 00:00:00
abstract::Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00454448
更新日期:1979-01-01 00:00:00
abstract::A sensitive new fluorescent method is outlined for the study of peptide and amino acid transport processes. Studies on dipeptide transport in germinating barley (Hordeum vulgare.) embryos have provided evidence for accumulation of substrate against a concentration gradient, saturation kinetics, a pH optimum at pH 3.8,...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387928
更新日期:1977-01-01 00:00:00
abstract::Two lectins were purified by affinity chromatography from mature peanut (Arachis hypogaea L.) nodules, and compared with the previously characterised seed lectin of this plant. One of the nodule lectins was similar to the seed lectin in its molecular weight and amino-acid composition and ability to bind derivatives of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392475
更新日期:1988-11-01 00:00:00
abstract::Cycloheximide (CH) was applied selectively either to the shoot apex or by infiltration to the leaves of the long-day plant Hyoscyamus niger in order to investigate whether this inhibitor has an effect on the synthesis of a floral stimulus in the leaves. Treatment of the shoot apex with CH caused inhibition of the phot...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386028
更新日期:1973-03-01 00:00:00
abstract::A re-examination has been made of the supposed regulation by the axis of cotyledonary isocitrate lyase (EC 4.1.3.1) activity in Cucumis sativas L. and Cucurbita maxima Duch. and greening in cucumis. When precautions are taken to ensure that isolated cotyledons and cotyledons attached to the axis have equal access to o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388904
更新日期:1976-01-01 00:00:00
abstract::Light-grown dwarf peas were disbudded except for a single lateral bud, then transferred to darkness at 24° C. During the dark period the seedlings were irradiated daily for 5 or 7 min with R or FR. The buds exposed to R developed into shoots faster than those irradiated with FR. The R effect was FR reversible, and the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387631
更新日期:1971-06-01 00:00:00
abstract::Furfural uptake was studied with regard to possible mechanisms of inducing germination in ascospores. Uptake was found to involve a large, weakly bound reversible component and a small tightly bound irreversible component. Localization experiments indicate that almost all of the furfural removed from the media is boun...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385758
更新日期:1970-12-01 00:00:00
abstract::Some botanical aspects of cell-free protein synthesizing systems from cereal embryos have been investigated. The composition of the starting material determines both the stability and translation fidelity of the cell-free extracts. The active components of the extracts originate exclusively from the primary axes. Cont...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00379838
更新日期:1980-01-01 00:00:00
abstract::The role of oxygen in the photoinactivation of the photosynthetic apparatus of Spinacia oleracea L. was investigated. Moderate irradiation (1200 μmol photons m(-2)s(-1)) of spinach leaves in an atmosphere of pure nitrogen caused strong inhibition of subsequently measured net CO2 assimilation, whereas considerably less...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392242
更新日期:1985-08-01 00:00:00
abstract::Soluble proteins from haploid gametophytes and diploid sporophytes of the marine green alga Ulva mutabilis Føyn have been reexamined, using polyacrylamide gel electrophoresis and isoelectric focusing. A two-dimensional system resolved about 150 protein spots. In contrast to an earlier report (Hoxmark (1976) Planta 130...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393447
更新日期:1982-11-01 00:00:00
abstract::The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0308-y
更新日期:2006-11-01 00:00:00
abstract::The possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing bacteria on ethylene production in canola (Brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. The effects of auxin (indol...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0532-0
更新日期:2007-09-01 00:00:00
abstract::Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394488
更新日期:1988-01-01 00:00:00
abstract:MAIN CONCLUSION:A large-scale bioinformatics analysis revealed the origin and evolution of GT47 gene family, and identified two clades of intron-poor genes with putative functions in drought stress responses and seed development in maize. Glycosyltransferase family 47 (GT47) genes encode β-galactosyltransferases and β-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2821-6
更新日期:2018-03-01 00:00:00
abstract::A delay of 20 to 30 min precedes a rapid increase in the production of ethylene when segments are excised from the petioles of tomato plants. Measurements made during this early phase may be better estimates of production by petioles on the intact plant than those made at later times. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398271
更新日期:1976-01-01 00:00:00