Abstract:
:The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax seed development. This fitted well with the tissue localization monitored by semi-quantitative RT-PCR of LuPLR expression. Accumulation of the main flax lignan secoisolariciresinol diglucoside was coherent with LuPLR expression during seed development. This three-way approach demonstrated that the LuPLR gene is expressed in the seed coat of flax seeds, and that the synthesis of PLR enzyme occurs where flax main lignan is found stored in mature seeds, confirming its involvement in SDG synthesis.
journal_name
Plantajournal_title
Plantaauthors
Hano C,Martin I,Fliniaux O,Legrand B,Gutierrez L,Arroo RR,Mesnard F,Lamblin F,Lainé Edoi
10.1007/s00425-006-0308-ysubject
Has Abstractpub_date
2006-11-01 00:00:00pages
1291-301issue
6eissn
0032-0935issn
1432-2048journal_volume
224pub_type
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