Pinoresinol-lariciresinol reductase gene expression and secoisolariciresinol diglucoside accumulation in developing flax (Linum usitatissimum) seeds.

Abstract:

:The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax seed development. This fitted well with the tissue localization monitored by semi-quantitative RT-PCR of LuPLR expression. Accumulation of the main flax lignan secoisolariciresinol diglucoside was coherent with LuPLR expression during seed development. This three-way approach demonstrated that the LuPLR gene is expressed in the seed coat of flax seeds, and that the synthesis of PLR enzyme occurs where flax main lignan is found stored in mature seeds, confirming its involvement in SDG synthesis.

journal_name

Planta

journal_title

Planta

authors

Hano C,Martin I,Fliniaux O,Legrand B,Gutierrez L,Arroo RR,Mesnard F,Lamblin F,Lainé E

doi

10.1007/s00425-006-0308-y

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

1291-301

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

224

pub_type

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