A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves.

Abstract:

:A monoclonal antibody produced to abscisic acid (ABA) has been characterised and the development of a radioimmunoassay (RIA) for ABA using the antibody is described. The antibody had a high selectivity for the free acid of (S)-cis, trans-ABA. Using the antibody, ABA could be assayed reliably in the RIA over a range from 100 to 4000 pg (0.4 to 15 pmol) ABA per assay vial. As methanol and acetone affected ABA-antibody binding, water was used to extract ABA from leaves. Water was as effective as aqueous methanol and acetone in extracting the ABA present. Crude aqueous extracts of wheat, maize and lupin leaves could be analysed without serious interference from other immunoreactive material. This was shown by measuring the distribution of immunoreactivity in crude extracts separated by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), or by comparing the assay with physicochemical methods of analysis. Analysis of crude extracts by RIA and either, after TLC purification, by gas chromatography using an electron-capture detector or, after HPLC purification, by combined gas chromatography-mass spectrometry (GC-MS) gave very similar ABA concentrations in the initial leaf samples. However, RIA analysis of crude aqueous extracts of pea seeds resulted in considerable overestimation of the amount of ABA present. Determinations of ABA content by GC-MS and RIA were similar after pea seed extracts had been purified by HPLC. Although the RIA could not be used to analyse ABA in crude extracts of pea seeds, it is likely that crude extracts of leaves of several other species may be assayed successfully.

journal_name

Planta

journal_title

Planta

authors

Quarrie SA,Whitford PN,Appleford NE,Wang TL,Cook SK,Henson IE,Loveys BR

doi

10.1007/BF00401020

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

330-9

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

173

pub_type

杂志文章

相关文献

PLANTA文献大全
  • [Flower initiation by cycloheximide in the long-day plant Hyoscyamus niger during short-days].

    abstract::Cycloheximide (CH) was applied selectively either to the shoot apex or by infiltration to the leaves of the long-day plant Hyoscyamus niger in order to investigate whether this inhibitor has an effect on the synthesis of a floral stimulus in the leaves. Treatment of the shoot apex with CH caused inhibition of the phot...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386028

    authors: Klautke S,Rau W

    更新日期:1973-03-01 00:00:00

  • Transcriptomic changes in wind-exposed poplar leaves are dependent on developmental stage.

    abstract::Responses of plant tissue to environmental challenges can vary among different plant parts and among plants of different ages. Investment into defense has been proposed to be influenced by fitness value and/or allocation of available resources. Here we show at first time at transcriptome level that plant defense is no...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-008-0777-2

    authors: Fluch S,Olmo CC,Tauber S,Stierschneider M,Kopecky D,Reichenauer TG,Matusíková I

    更新日期:2008-10-01 00:00:00

  • Analysis of storage proteins in normal and aborted seeds from embryo-lethal mutants of Arabidopsis thaliana.

    abstract::The major storage proteins isolated from wild-type seeds of Arabidopsis thaliana (L.) Heynh., strain "Columbia", were studied by sucrose gradient centrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Both the hypocotyl and cotyledons of mature embryos contained abundant 12 S (crucife...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392124

    authors: Heath JD,Weldon R,Monnot C,Meinke DW

    更新日期:1986-11-01 00:00:00

  • Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots.

    abstract::Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthet...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0461-3

    authors: Innocenti G,Pucciariello C,Le Gleuher M,Hopkins J,de Stefano M,Delledonne M,Puppo A,Baudouin E,Frendo P

    更新日期:2007-05-01 00:00:00

  • The role of gibberellin in hypocotyl extension of dark-growing Lupinus albus seedlings.

    abstract::Six authentic gibberellins marginally promoted hypocotyl lengthening of etiolated lupin seedlings whereas extracted lupin "gibberellin-like" compounds were ineffective. However, dwarfing by AMO-1618 [2'-isopropyl-4'-(trimethyl-ammonium chloride)-5'-methylphenyl piperidine carboxylate] was totally counteracted by co-ap...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388394

    authors: Murray PB,Acton GJ

    更新日期:1974-09-01 00:00:00

  • In-vivo study of cutin synthesis in leaves of Clivia miniata Reg.

    abstract::Cutin synthesis of Clivia miniata Reg. was studied by using intact leaves. Tritium-labelled hexadecanoic acid was used as precursor and was administered as droplets of micellar solutions to the upper surface of expanding leaves. Radiolabel was incorporated rapidly. Within 2 h, up to 10% of the label administered had b...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00395405

    authors: Lendzian KJ,Schönherr J

    更新日期:1983-06-01 00:00:00

  • An auxin-responsive 1-aminocyclopropane-1-carboxylate synthase is responsible for differential ethylene production in gravistimulated Antirrhinum majus L. flower stems.

    abstract::The regulation of gravistimulation-induced ethylene production and its role in gravitropic bending was studied in Antirrhinum majus L. cut flower stems. Gravistimulation increased ethylene production in both lower and upper halves of the stems with much higher levels observed in the lower half. Expression patterns of ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1359-6

    authors: Woltering EJ,Balk PA,Nijenhuis-Devries MA,Faivre M,Ruys G,Somhorst D,Philosoph-Hadas S,Friedman H

    更新日期:2005-01-01 00:00:00

  • Storage-protein regulation and lipid accumulation in microspore embryos of Brassica napus L.

    abstract::Embryos derived in vitro from isolated microspores of Brassica napus L. were compared with their zygotic counterparts. Parameters investigated included storage-protein accumulation and gene expression, fattyacid composition, storage-lipid biosynthesis, and the appearance of oil-body proteins. The microspore embryos ac...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00202320

    authors: Taylor DC,Weber N,Underhill EW,Pomeroy MK,Keller WA,Scowcroft WR,Wilen RW,Moloney MM,Holbrook LA

    更新日期:1990-04-01 00:00:00

  • "Arabidobrassica": Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells.

    abstract::A somatic hybrid cell line, cloned from an individual protoplast-fusion product between Arabidopsis thaliana and Brassica campestris, gave rise to formation of numerous plants differing drastically in morphology. Analysis of these various regenerants, all of which originated from one and the same heterokaryon derived ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385546

    authors: Hoffmann F,Adachi T

    更新日期:1981-12-01 00:00:00

  • Distinct non-target site mechanisms endow resistance to glyphosate, ACCase and ALS-inhibiting herbicides in multiple herbicide-resistant Lolium rigidum.

    abstract::This study investigates mechanisms of multiple resistance to glyphosate, acetyl-coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS)-inhibiting herbicides in two Lolium rigidum populations from Australia. When treated with glyphosate, susceptible (S) plants accumulated 4- to 6-fold more shikimic acid than r...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0981-8

    authors: Yu Q,Abdallah I,Han H,Owen M,Powles S

    更新日期:2009-09-01 00:00:00

  • "Arabidobrassica": A novel plant obtained by protoplast fusion.

    abstract::Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00380870

    authors: Gleba YY,Hoffmann F

    更新日期:1980-07-01 00:00:00

  • Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments.

    abstract::The effects of O2, growth-regulators and desiccation on callus growth and somatic embryo (embryoid) development were investigated in cultures of immature embryos of two lines of Triticum aestivum L. Callus and embryoid formation were induced on media that contained N(6)-furfurylamin-opurine (kinetin) and either 2,4-di...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00396349

    authors: Carman JG

    更新日期:1988-09-01 00:00:00

  • Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation.

    abstract::Tea seed is believed to be recalcitrant based on its sensitivity to chilling or drying stress. Reactive oxygen species (ROS) and alterations in cytosolic redox status have been implicated in intolerance to desiccation by recalcitrant seed, but there is little information available regarding how ROS are regulated in se...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1322-7

    authors: Chen Q,Yang L,Ahmad P,Wan X,Hu X

    更新日期:2011-03-01 00:00:00

  • Brassinolide may control aquaporin activities in Arabidopsis thaliana.

    abstract::It is usually assumed that aquaporins present in the cellular membranes could be an important route in the control of water flux in plants, but evidence for this hypothesis is scarce. In this paper, we report measurements of the osmotic permeability (P(os) of protoplasts isolated from hypocotyls of wild-type and mutan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000379

    authors: Morillon R,Catterou M,Sangwan RS,Sangwan BS,Lassalles JP

    更新日期:2001-01-01 00:00:00

  • Fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides.

    abstract:MAIN CONCLUSION:Physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. Changes in the physical properties of plant cell walls, a viscoelastic structure, are th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2176-1

    authors: Takahashi M,Yamamoto R,Sakurai N,Nakano Y,Takeda T

    更新日期:2015-02-01 00:00:00

  • Purification and properties of an endo-beta-1,4-glucanase from strawberry and down-regulation of the corresponding gene, cel1.

    abstract::An endo-beta-1,4-glucanase (EG) was purified from ripe strawberry (Fragaria x ananassa Duch.) fruit using cellulose affinity chromatography. The purified enzyme gave a single protein band of 54 kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of this protein showed ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100577

    authors: Woolley LC,James DJ,Manning K

    更新日期:2001-11-01 00:00:00

  • Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

    abstract:MAIN CONCLUSION:Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spre...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-018-03084-0

    authors: Lambein F,Travella S,Kuo YH,Van Montagu M,Heijde M

    更新日期:2019-09-01 00:00:00

  • Calmodulin and wound healing in the coenocytic green alga Ernodesmis verticillata (Kützing) Børgesen: Ultrastructure of the cortical cytoskeleton and immunogold labeling.

    abstract::Ultrastructural changes in the cortical cytoskeleton during wound-induced cytoplasmic contraction were examined in the coenocytic green alga Ernodesmis verticillata. Both calmodulin (CaM) and actin were localized in intact and contracting cells by immunogold labeling. Within 5 min after wounding, compact microfilament...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201493

    authors: Goddard RH,La Claire JW 2nd

    更新日期:1991-12-01 00:00:00

  • The evolutionary landscape of PRC1 core components in green lineage.

    abstract:MAIN CONCLUSION:The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. It is ...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-015-2451-9

    authors: Chen DH,Huang Y,Ruan Y,Shen WH

    更新日期:2016-04-01 00:00:00

  • Involvement of indole-3-acetic acid in the circadian growth of the first internode of Arabidopsis.

    abstract::The extension rate of the first inflorescence node of Arabidopsis was measured during light/dark or continuous light exposure and was found to exhibit oscillations which showed a circadian rhythmicity. Decapitation induced a strong inhibition of stem extension. Subsequent application of IAA restored growth and the ass...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050615

    authors: Jouve L,Gaspar T,Kevers C,Greppin H,Degli Agosti R

    更新日期:1999-07-01 00:00:00

  • Comparative transcriptomic analysis between 'Summer Black' and its bud sport 'Nantaihutezao' during developmental stages.

    abstract:MAIN CONCLUSION:4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played essential roles in contributing to the total soluble solid and total anthocyanin variations induced by bud sport in grape berries. Taste and color, which are important organoleptic qualities of grape berry, un...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03543-7

    authors: Leng F,Ye Y,Zhu X,Zhang Y,Zhang Z,Shi J,Shen N,Jia H,Wang L

    更新日期:2021-01-05 00:00:00

  • The role of cysteine proteinase and carboxypeptidase in the breakdown of storage proteins in buckwheat seeds.

    abstract::Two proteolytic enzymes, a cysteine proteinase and a carboxypeptidase, responsible for breakdown of the main storage protein, 13S globulin, were purified from buckwheat seedlings (Fagopyrum esculentum Moench) by (NH4)2SO4 fractionation, gel-filtration on Sephadex G-150, ionexchange chromatography on DEAE-Toyopearl 650...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391076

    authors: Dunaevsky YE,Belozersky MA

    更新日期:1989-10-01 00:00:00

  • Cloning and expression of an arginine decarboxylase cDNA from Vitis vinifera L. cell-suspension cultures.

    abstract::Arginine decarboxylase (ADC; EC 4.1.1.19) is a key enzyme in one of the two pathways to putrescine. We present the first ADC cDNA from a woody perennial plant species, the grapevine (Vitis vinifera L.), which exhibits 70-80% homology with other dicot ADCs. The effects of ammonium, nitrate, and putrescine on ADC specif...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050595

    authors: Primikirios NI,Roubelakis-Angelakis KA

    更新日期:1999-06-01 00:00:00

  • Reorganization of the endoplasmic reticulum in epidermal cells of onion bulb scales after cold stress: Involvement of cytoskeletal elements.

    abstract::In the epidermal cells of onion (Allium cepa L.) bulb scales the endoplasmic reticulum (ER) can be subdivided into three domains: a peripheral tubular network, cisternae, and long tubular strands. The latter are the form in which the ER is moved in onion cells. During cold treatment the arrangement of the three domain...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392816

    authors: Quader H,Hofmann A,Schnepf E

    更新日期:1989-02-01 00:00:00

  • Functional testing of a PF02458 homologue of putative rice arabinoxylan feruloyl transferase genes in Brachypodium distachyon.

    abstract:MAIN CONCLUSION:We show that changing the expression of a putative feruloyl transferase gene belonging to the BAHD acyl-transferase family alters the levels of cell wall esterified ferulates and diferulates in Brachypodium distachyon cell walls. While the potential of grass cell walls for biofuel production has been re...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-015-2430-1

    authors: Buanafina MM,Fescemyer HW,Sharma M,Shearer EA

    更新日期:2016-03-01 00:00:00

  • A single gene inherited trait confers metabolic resistance to chlorsulfuron in grain sorghum (Sorghum bicolor).

    abstract:MAIN CONCLUSION:This study confirms a high level of metabolic resistance to the herbicide chlorsulfuron, inherited by a single dominant gene in a sorghum genotype (GL-1). Chlorsulfuron, an acetolactate synthase (ALS)-inhibitor, effectively controls post-emergence grass and broadleaf weeds but is not registered for use ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03563-3

    authors: Pandian BA,Sathishraj R,Prasad PVV,Jugulam M

    更新日期:2021-01-23 00:00:00

  • Lipases in the storage tissues of peanut and other oil seeds during germination.

    abstract::The castor-bean endosperm-the best-studied material of reserve lipid hydrolysis in seed germination-was previously shown to have an acid lipase and an alkaline lipase having reciprocal patterns of development during germination. We studied oil seeds from 7 species, namely castor bean (Ricinus communis L.), peanut (Ara...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387752

    authors: Huang AH,Moreau RA

    更新日期:1978-01-01 00:00:00

  • The effect of vomifoliol on stomatal aperture.

    abstract::Vomifoliol, a compound related to abscisic acid (ABA) but lacking the 2,4-pentadiene sidechain and so far found in four different plant families, caused as much stomatal closure in epidermal strips of Eichhornia crassipes (Mart.) Solms as ABA at identical concentrations. It appears worthwhile to investigate whether vo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385281

    authors: Stuart KL,Coke LB

    更新日期:1975-01-01 00:00:00

  • Further observations on cell-wall formation around isolated protoplasts of tobacco and tomato.

    abstract::Freeze-etch observations of protoplasts isolated from tobacco (Nicotiana tabacum L.) mesophyll tissue and tomato (Lycopersicum esculentum Mill.) fruit locule tissue are described which clarify earlier observations (Burgess, J., Fleming, E.N., Planta 131, 173-178, 1976; Planta 133, 267-273, 1977), obtained using scanni...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00389379

    authors: Willison JH,Grout BW

    更新日期:1978-01-01 00:00:00

  • Heterogeneous inhibition of photosynthesis over the leaf surface of rosa rubiginosa L. during water stress and abscisic acid treatment: induction of a metabolic component by limitation of CO(2) diffusion

    abstract::The contribution of changes in stomatal conductance and metabolism in determining heterogeneous photosynthesis inhibition during dehydration and abscisic acid (ABA) feeding was investigated using detached leaves of Rosa rubiginosa L. The steady-state and maximal rates of electron transport under a transient high CO(2)...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050661

    authors: Meyer S,Genty B

    更新日期:1999-11-01 00:00:00