In-vivo study of cutin synthesis in leaves of Clivia miniata Reg.

Abstract:

:Cutin synthesis of Clivia miniata Reg. was studied by using intact leaves. Tritium-labelled hexadecanoic acid was used as precursor and was administered as droplets of micellar solutions to the upper surface of expanding leaves. Radiolabel was incorporated rapidly. Within 2 h, up to 10% of the label administered had been incorporated into cutin. Rates of (3)H-cutin synthesis depended on the position of the site of precursor donation to the leaf. Highest rates were observed between 3 to 4 cm from the leaf base. From zero to 3 cm, rates increased by about one order of magnitude every centimeter. Above 4 cm, the decrease in rates of (3)H-cutin synthesis was again logarithmic, such that at 10 cm from leaf base only 1%, and at 15 cm from leaf base only 0.1% of the maximum rates were observed. Rates of cutin synthesis depended on the hexadecanoic acid concentration of the droplets, according to the Michaelis-Menten equation. The maximum rate was 0.71 μg cm(-2) h(-1). The half-maximum rate was observed at a hexadecanoic acid concentration of 42.4 mg l(-1). Maximal cutin synthesis coincided with maximal cell elongation. Microautoradiography indicated that most of the label was incorporated into the internal cuticular layer.

journal_name

Planta

journal_title

Planta

authors

Lendzian KJ,Schönherr J

doi

10.1007/BF00395405

subject

Has Abstract

pub_date

1983-06-01 00:00:00

pages

70-5

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

158

pub_type

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