Lipases in the storage tissues of peanut and other oil seeds during germination.

abstract：:Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica napus L. AAPT1 gene (designated BnAAPT1) was identified from cDNA libraries of seedlings and developing se...

journal_title：Planta

authors： Qi Q,Huang YF,Cutler AJ,Abrams SR,Taylor DC

更新日期：2003-08-01 00:00:00

abstract：:Calli were initiated from carrot (Daucus carota L. subsp. sativus Hoffm.) root expiants and grown on media supplemented with a combination of carbenicillin at 0.3 mg/ml and vancomycin at 0.1 mg/ml in the absence of plant hormones or hormone analogs. The growth rate was about half of that obtained with a combination of...

journal_title：Planta

authors： Chang CC,Schmidt DR

更新日期：1991-11-01 00:00:00

abstract：:Determination of appropriate reference genes is crucial to normalization of gene expression data and prevention of biased results in qRT-PCR studies. This study is the first attempt to systematically compare potential reference genes to detect the most constitutively expressed reference genes for accurate normalizatio...

journal_title：Planta

authors： Mehdi Khanlou K,Van Bockstaele E

更新日期：2012-11-01 00:00:00

abstract：:The recent introduction of (31)P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870-872; 1981,...

journal_title：Planta

authors： Aducci P,Federico R,Carpinelli G,Podo F

更新日期：1982-01-01 00:00:00

abstract：:The green alga Chlorella zofingiensis can accumulate high level of oleic acid (OA, C18:1△(9)) rich oils in response to stress conditions. To understand the regulation of biosynthesis of fatty acid in particular OA at the molecular level, we cloned and characterized the stearoyl acyl carrier protein (ACP) desaturase (S...

journal_title：Planta

authors： Liu J,Sun Z,Zhong Y,Huang J,Hu Q,Chen F

更新日期：2012-12-01 00:00:00

abstract：:A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8...

journal_title：Planta

authors： Scheer JM,Pearce G,Ryan CA

更新日期：2005-07-01 00:00:00

abstract：:The endosperm of Euphorbia lagascae Spreng. seeds contains high levels of the epoxidated fatty acid vernolic acid ( cis-12-epoxyoctadeca-cis-9-enoic acid). To obtain transgenic oilcrops producing high levels of vernolic acid, better knowledge of its endogenous metabolism is needed. In this paper we study the gene acti...

journal_title：Planta

authors： Edqvist J,Farbos I

更新日期：2002-05-01 00:00:00

abstract：:Fatty acid synthesis was studied in successive leaf sections from the base to the tip of developing barley (Hordeum vulgare L.), maize (Zea mays L.), rye grass (Lolium perenne L.) and wheat (Triticum aestivium L.) leaves. The basal regions of the leaves had the lowest rates of fatty acid synthesis and accumulated smal...

journal_title：Planta

authors： Bolton P,Harwood JL

更新日期：1978-01-01 00:00:00

abstract：:Seven endochitinases (EC 3.2.1.14) (relative molecular masses 23,000-28,000 and isoelectric points 10.3-10.4) were purified from nonembryogenic Citrus sinensis L. Osbeck cv. Valencia callus tissue. The basic chitinase/lysozyme from this tissue (BCLVC) exhibited lysozyme, chitinase and chitosanase activities and was de...

journal_title：Planta

authors： Mayer RT,McCollum TG,Niedz RP,Hearn CJ,McDonald RE,Berdis E,Doostdar H

更新日期：1996-01-01 00:00:00

abstract：MAIN CONCLUSION:The aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsRNA with sequence identity to the nematode gene in tobacco roots under the influence of strong Arabidopsis ubiquitin (UBQ1) promoter. Root-knot nematodes (RKN...

journal_title：Planta

authors： Ajjappala H,Chung HY,Sim JS,Choi I,Hahn BS

更新日期：2015-03-01 00:00:00

abstract：:Aspartate kinase (EC 2.7.2.4.) has been purified from 7 day etiolated wheat (Triticum aestivum L. var. Maris Freeman) seedlings and from embryos imbibed for 8 h. The enzyme was 50% inhibited by 0.25 mM lysine. In this study wheat aspartate kinase was not inhibited by threonine alone or cooperatively with lysine; these...

journal_title：Planta

authors： Bright SW,Shewry PR,Miflin BJ

更新日期：1978-01-01 00:00:00

abstract：:To explore the feasibility of immunolocalisation of endogenous abscisic acid (ABA), model systems were developed for testing quantitatively the sensitivity of the second antibody peroxidase/antiperoxidse (PAP) method for immunolocalisation of ABA on plant tissues. Exogenous (±)ABA was fixed to carrot sections on glass...

journal_title：Planta

authors： Skene DS,Browning G,Jones HG

更新日期：1987-10-01 00:00:00

abstract：:Oxygen and CO2 exchange were measured concurrently in leaves of shade-grownAlocasia macrorrhiza (L.) G. Don during lightflecks consisting of short periods of high photon flux density (PFD) superimposed on a low-PFD background illumination. Oxygen exchange was measured with a zirconium-oxide ceramic cell in an atmosphe...

journal_title：Planta

authors： Kirschbaum MU,Pearcy RW

更新日期：1988-12-01 00:00:00

abstract：MAIN CONCLUSION:Our work strongly suggests that microRNA858 regulates anthocyanin biosynthesis in tomato by modulating the expression of two R2R3 MYB transcription factors, underscoring the importance of microRNAs in the gene regulatory network controlling specialized metabolism in plants. The biological functions of m...

journal_title：Planta

authors： Jia X,Shen J,Liu H,Li F,Ding N,Gao C,Pattanaik S,Patra B,Li R,Yuan L

更新日期：2015-07-01 00:00:00

abstract：:Photosynthesis of Helianthus annuus L. leaves was transiently inhibited and respiration was stimulated when a leaf was detached from the plant by cutting the petiole under water. These effects were caused by a solute which was released by cutting and was transported by the transpiration stream to the leaf blade. This ...

journal_title：Planta

authors： Gsell W,Kiirats O,Hartung W,Heber U

更新日期：1989-03-01 00:00:00

abstract：:Measurements of net CO2 exchange, malate accumulation, properties and capacity of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in leaves of different ages of two short-day dependent Crassulacean acid metabolism (CAM) plants (Kalanchoe blossfeldiana v. Poelln. Tom thumb and K. velutina Welw.) show that, in both ...

journal_title：Planta

authors： Brulfert J,Guerrier D,Queiroz O

更新日期：1982-05-01 00:00:00

abstract：:Water and solute relations of excised seminal roots of young maize (Zea mays L) plants, have been measured using the root pressure probe. Upon addition of osmotic solutes to the root medium, biphasic root pressure relaxations were obtained as theoretically expected. The relaxations yielded the hydraulic conductivity L...

journal_title：Planta

authors： Steudle E,Frensch J

更新日期：1989-03-01 00:00:00

abstract：:Stable-isotope discrimination factors (D) for the uptake of oxygen during respiration by a variety of plant materials were determined by measuring (18)O enrichment in a closed system. Baker's yeast (Saccharomyces cerevisiae Meyer) and mitochondrial preparations from baker's yeast and from castor bean (Ricinus communis...

journal_title：Planta

authors： Guy RD,Berry JA,Fogel ML,Hoering TC

更新日期：1989-04-01 00:00:00

abstract：:Changes in fatty-acid metabolism were studied in soybean (Glycine max Merr.) cotyledons during senescence as well as in cotyledons which had been caused to regreen by removal of the epicotyl from the seedling. The activities of the enzymes acetyl-CoA synthetase (EC 6.2.1.1) and fatty-acid synthetase in plastids isolat...

journal_title：Planta

authors： Place MA,Morgan MS,Rutkoski A,Newman DW,Jaworski JG

更新日期：1979-12-01 00:00:00

abstract：:The occurrence of heterotrophic nitrification in nitrogen-starved cells of Ankistrodesmus braunii was confirmed. The levels of nitrate and nitrite were measured over a period of four weeks. The validity of quantitative determinations in the presence of highly active nitrate and nitrite reductases is discussed. Whereas...

journal_title：Planta

authors： Spiller H,Dietsch E,Kessler E

更新日期：1976-01-01 00:00:00

abstract：:The vacuolar pH of individual cells of the epidermis of intact leaves of Commelina communis L. has been determined using pH sensitive microelectrodes. The results show that the pH of the guard cells increases by approximately 0.5 units during stomatal opening. The changes in vacuolar pH mirror the changes in vacuolar ...

journal_title：Planta

authors： Penny MG,Bowling DJ

更新日期：1975-01-01 00:00:00

abstract：:During anaerobiosis in darkness the main route for ATP production in plants is through glycolysis in combination with fermentation. We compared the organ-specific anaerobic fermentation of flooding-tolerant rice (Oryza sativa) and sensitive wheat (Triticum aestivum) seedlings. A sensitive laser-based photoacoustic tra...

journal_title：Planta

authors： Mustroph A,Boamfa EI,Laarhoven LJ,Harren FJ,Albrecht G,Grimm B

更新日期：2006-12-01 00:00:00

abstract：:The differential growth causing second positive phototropic curvature in intact, black-capped and decapitated Avena coleoptiles has been measured. In all cases the curvature is brought about by a cessation in growth of the illuminated side. The fact that shading the apex does not significantly alter the initial steps ...

journal_title：Planta

authors： Franssen JM,Firn RD,Digby J

更新日期：1982-08-01 00:00:00

abstract：:In plants of Silene coeli-rosa, induced to flower by 7 LD, synchronisation of cell division in 20 per cent or more of the cells in the shoot apical dome was found on the 8th and 9th days after the beginning of induction, during the plastochron before sepal initiation. Synchronisation was inferred from the changes in t...

journal_title：Planta

authors： Francis D,Lyndon RF

更新日期：1979-01-01 00:00:00

abstract：:Spores of the ferns, Dryopteris filix-mas, D. paleacea and Polystichum minutum, sown on plain agar in quartz-distilled water, required several hours of red light in order to germinate. When, however, water agar was replaced by agar made up with a mineral nutrition medium, a single pulse of red light (about 1 min) was ...

journal_title：Planta

authors： Haupt W

更新日期：1985-05-01 00:00:00

abstract：:Mitochondria were isolated from tomato (Lycopersicon esculentum L.) fruit at the mature green, orange-green and red stages and from fruit artificially suspended in their ripening stage. The specific activities of citrate synthase (EC 4.1.3.7), malate dehydrogenase (EC 1.1.1.37), NAD-linked isocitrate dehydrogenase (EC...

journal_title：Planta

authors： Jeffery D,Goodenough PW,Weitzman PD

更新日期：1986-09-01 00:00:00

abstract：:The storage-protein content of non-zygotic and zygotic embryos of B. napus was compared, using antibodies to guantitate 12S storage protein in extracts by rocket immunoelectrophoresis. Non-zygotic embryos were induced from microspores in anther culture and on the hypocotyls of zygotic embryos in culture. All embryo-li...

journal_title：Planta

authors： Crouch ML

更新日期：1982-01-01 00:00:00

abstract：:The relationship between growth, change in cell osmotic pressure and accumulation of osmotic solutes was investigated in hypocotyls of sunflower (Helianthus annum L.) seedlings. During growth in darkness the osmotic pressure decreased by 50% between days 2 and 6 after sowing. After irradiation of dark-grown seedlings ...

journal_title：Planta

authors： Kutschera U

更新日期：1991-04-01 00:00:00

abstract：:The profile of (14)C, that is the plot of log (radioactivity) against distance, down the stem of Helianthus seedlings was studied over the course of the first hour after feeding (14)C to a leaf. These seedlings are shown to accumulate a large proportion of the total (14)C present in the stem in an ethanol insoluble fo...

journal_title：Planta

authors： Whittle CM

更新日期：1970-09-01 00:00:00

abstract：:Transpiration from excised leaves of Anthephra pubescens Nees was enhanced by 1 and 10 mmol m(-3) kinetin. Stomatal opening in isolated epidermal strips of A. pubescens under CO2-free air and in the absence of K(+) was enhanced by 10 mmol m(-3) kinetin. ...

journal_title：Planta

authors： Incoll LD,Whitelam GC

更新日期：1977-01-01 00:00:00