Non-zygotic embryos of Brassica napus L. contain embryo-specific storage proteins.

Abstract:

:The storage-protein content of non-zygotic and zygotic embryos of B. napus was compared, using antibodies to guantitate 12S storage protein in extracts by rocket immunoelectrophoresis. Non-zygotic embryos were induced from microspores in anther culture and on the hypocotyls of zygotic embryos in culture. All embryo-like structures were found to contain 12S storage protein, whereas preculture anthers, anthers from which embryos had been removed, and regenerated shoots did not have detectable 12S storage protein. In zygotic embryos, 12S storage protein was first detected at the cotyledon stage, but microsporic embryos contained storage protein at the globular and heart stages. Storage protein levels in microsporic and hypocotyl embryos were low relative to those in zygotic embryos. The largest microsporic embryo had a storage protein concentration of 13 μg mg(-1) fresh weight, almost 10 times lower than a mature zygotic embryo. Thus, although storage proteins are present in both zygotic and non-zygotic embryos, the timing and extent of accumulation differ.

journal_name

Planta

journal_title

Planta

authors

Crouch ML

doi

10.1007/BF00392774

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

520-4

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

156

pub_type

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