The role of beta-ketoacyl-acyl carrier protein synthase III in the condensation steps of fatty acid biosynthesis in sunflower.

Abstract:

:The beta-ketoacyl-acyl carrier protein synthase III (KAS III; EC 2.3.1.180) is a condensing enzyme catalyzing the initial step of fatty acid biosynthesis using acetyl-CoA as primer. To determine the mechanisms involved in the biosynthesis of fatty acids in sunflower (Helianthus annuus L.) developing seeds, a cDNA coding for HaKAS III (EF514400) was isolated, cloned and sequenced. Its protein sequence is as much as 72% identical to other KAS III-like ones such as those from Perilla frutescens, Jatropha curcas, Ricinus communis or Cuphea hookeriana. Phylogenetic study of the HaKAS III homologous proteins infers its origin from cyanobacterial ancestors. A genomic DNA gel blot analysis revealed that HaKAS III is a single copy gene. Expression levels of this gene, examined by Q-PCR, revealed higher levels in developing seeds storing oil than in leaves, stems, roots or seedling cotyledons. Heterologous expression of HaKAS III in Escherichia coli altered their fatty acid content and composition implying an interaction of HaKAS III with the bacterial FAS complex. Testing purified HaKAS III recombinant protein by adding to a reconstituted E. coli FAS system lacking condensation activity revealed a novel substrate specificity. In contrast to all hitherto characterized plant KAS IIIs, the activities of which are limited to the first cycles of intraplastidial fatty acid biosynthesis yielding C6 chains, HaKAS III participates in at least four cycles resulting in C10 chains.

journal_name

Planta

journal_title

Planta

authors

González-Mellado D,von Wettstein-Knowles P,Garcés R,Martínez-Force E

doi

10.1007/s00425-010-1131-z

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

1277-89

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

231

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Towards the development of an oral vaccine against porcine cysticercosis: expression of the protective HP6/TSOL18 antigen in transgenic carrots cells.

    abstract:MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-015-2431-0

    authors: Monreal-Escalante E,Govea-Alonso DO,Hernández M,Cervantes J,Salazar-González JA,Romero-Maldonado A,Rosas G,Garate T,Fragoso G,Sciutto E,Rosales-Mendoza S

    更新日期:2016-03-01 00:00:00

  • A barley gene (rsh1) encoding a ribonuclease S-like homologue specifically expressed in young light-grown leaves.

    abstract::A group of frequent cDNA clones from a young-leaf cDNA library was found to code for a homologue of S-ribonucleases (S-RNases) involved in gametophytic incompatibility and the so-called S-like RNases active in flowers and in vegetative tissues. The derived amino acid sequence starts with a signal peptide and has a 27-...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050046

    authors: Gausing K

    更新日期:2000-03-01 00:00:00

  • Hormone treatment of roots causes not only a reversible loss of starch but also of structural polarity in statocytes.

    abstract::Treatment of cress (Lepidium sativum L.) roots with phytohormones (4.3 x 10(-5) M gibberellic acid plus 4.3 x 10(-5) M kinetin, 30 h; T.H. Iversen, 1969, Physiol. Plant. 22, 1251-1262) caused not only complete destarching of amyloplasts but also destruction of the polar arrangement of cell organelles in statocytes. Th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00195888

    authors: Busch MB,Sievers A

    更新日期:1990-06-01 00:00:00

  • Effects of 2,4-dinitrophenol and anoxia on the inorganic-pyrophosphate content of the spadix of Arum maculatum and the root apices of Pisum sativum.

    abstract::This work was done to determine whether the inorganic-pyrophosphate (PPi) content of plant tissues changes when the rate of glycolysis is altered. Treatment of excised clubs of the spadix of Arum maculatum L. and root apices of Pisum sativum L. with 2,4-dinitrophenol increased the rates of respiration but had no detec...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391871

    authors: Dancer JE,Rees TA

    更新日期:1989-06-01 00:00:00

  • Involvement of indole-3-acetic acid in the circadian growth of the first internode of Arabidopsis.

    abstract::The extension rate of the first inflorescence node of Arabidopsis was measured during light/dark or continuous light exposure and was found to exhibit oscillations which showed a circadian rhythmicity. Decapitation induced a strong inhibition of stem extension. Subsequent application of IAA restored growth and the ass...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050615

    authors: Jouve L,Gaspar T,Kevers C,Greppin H,Degli Agosti R

    更新日期:1999-07-01 00:00:00

  • Isolation of novel types of Arabidopsis mutants with altered reactions to cadmium: cadmium-gradient agar plates are an effective screen for the heavy metal-related mutants.

    abstract::We are interested in elucidating the molecular mechanisms underlying plant reactions to the toxic heavy metal cadmium (Cd). To this end, we devised a new screening strategy using agar plates with a gradient of Cd concentrations, termed Cd-gradient agar plates (CGAPs), to isolate Arabidopsis mutants that displayed alte...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1217-7

    authors: Watanabe A,Ito H,Chiba M,Ito A,Shimizu H,Fuji S,Nakamura S,Hattori H,Chino M,Satoh-Nagasawa N,Takahashi H,Sakurai K,Akagi H

    更新日期:2010-09-01 00:00:00

  • Nitrate inhibits the remobilization of cell wall phosphorus under phosphorus-starvation conditions in rice (Oryza sativa).

    abstract:MAIN CONCLUSION:NO3- not only inhibited the reutilization of cell wall P via decreasing root cell wall pectin content and PME activity, but also hampered the P translocation from root to shoot. The rice cultivars 'Kasalath' (Kas) and 'Nipponbare' (Nip) were used to demonstrate that the nitrogen source NO3- inhibits int...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2892-z

    authors: Zhu CQ,Zhu XF,Wang C,Dong XY,Shen RF

    更新日期:2018-07-01 00:00:00

  • Role of ethylene in phytochrome-induced anthocyanin synthesis.

    abstract::Synthesis of anthocyanin pigments in etiolated cabbage seedlings is influenced by ethylene at concentrations higher than 10 ppb, and etiolated seedlings produce sufficient ethylene to influence their anthocyanin synthesis. When escape of endogenous ethylene from this tissue is enhanced by means of hypobaric treatment,...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387635

    authors: Kang BG,Burg SP

    更新日期:1973-09-01 00:00:00

  • Brassinosteroids, microtubules and cell elongation in Arabidopsis thaliana. II. Effects of brassinosteroids on microtubules and cell elongation in the bul1 mutant.

    abstract::In order to elucidate the involvement of brassinosteroids in the cell elongation process leading to normal plant morphology, indirect immunofluorescence and molecular techniques were use to study the expression of tubulin genes in the bul1-1 dwarf mutant of Arabidopsis thaliana (L.) Heynh., the characteristics of whic...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000467

    authors: Catterou M,Dubois F,Schaller H,Aubanelle L,Vilcot B,Sangwan-Norreel BS,Sangwan RS

    更新日期:2001-04-01 00:00:00

  • Further studies on the relationship between the rates of nitrate uptake, growth and conductivity changes in the medium of plant cell suspension cultures.

    abstract::The changes in packed cell volume and in nitrate content and conductivity of the medium during the growth cycle of cell suspension cultures from Petroselinum hortense Hoffm., Glycine max Merr., and Haplopappus gracilis A. Grey in a chemically defined medium were compared. In all three cases sigmoidal curves obtained f...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388694

    authors: Hahlbrock K

    更新日期:1975-01-01 00:00:00

  • Fatty-acid metabolism in senescing and regreening soybean cotyledons.

    abstract::Changes in fatty-acid metabolism were studied in soybean (Glycine max Merr.) cotyledons during senescence as well as in cotyledons which had been caused to regreen by removal of the epicotyl from the seedling. The activities of the enzymes acetyl-CoA synthetase (EC 6.2.1.1) and fatty-acid synthetase in plastids isolat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388746

    authors: Place MA,Morgan MS,Rutkoski A,Newman DW,Jaworski JG

    更新日期:1979-12-01 00:00:00

  • Measurement of internal pH in the coccolithophoreEmiliania huxleyi using 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein acetoxymethylester and digital imaging microscopy.

    abstract::Internal pH (pHi) was determined inEmiliania huxleyi (Lohmann) using the probe 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluoresceinacetoxymethylester (BCEF-AM) and digital imaging microscopy. The probe BCECF-AM was taken up and hydrolysed to the free acid by the cells. A linear relationship was established between pH...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00963813

    authors: Dixon GK,Brownlee C,Merrett MJ

    更新日期:1989-12-01 00:00:00

  • Influence of certain environmental factors on photosynthesis and photorespiration in Simmondsia chinensis.

    abstract::The response of net photosynthesis and apparent light respiration to changes in [O2], light intensity, and drought stress was determined by analysis of net photosynthetic CO2 response curves. Low [O2] treatment resulted in a large reduction in the rate of photorespiratory CO2 evolution. Lightintensity levels influence...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384961

    authors: Collatz GJ

    更新日期:1977-01-01 00:00:00

  • Chitinase production in pine callus (Pinus sylvestris L.): a defense reaction against endophytes?

    abstract::In shoot tip-derived tissue cultures of Scots pine (Pinus sylvestris L.), browning and subsequent degeneration of the culture is accompanied by lipid peroxidation and lignification of cells, which are characteristic features of a plant defense reaction. Since chitinases are enzymes acting primarily in plant defense, t...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-001-0709-x

    authors: Pirttilä AM,Laukkanen H,Hohtola A

    更新日期:2002-04-01 00:00:00

  • Photosynthetic activity of isolated chloroplasts from Euglena gracilis.

    abstract::Functional chloroplasts from photoheterotrophic Euglena gracilis can be isolated in isoosmotic gradients of 10-80% Percoll. The chloroplasts display rates of CO2 dependent O2 evolution and CO2 fixation of 30-50 μmol mg(-1) chlorophyll h(-1) or 25-35% of the net O2 evolution by the whole cells and appear to be striking...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385499

    authors: Schürmann P,Ortiz W

    更新日期:1982-03-01 00:00:00

  • Manganese superoxide dismutase from a higher plant : Purification of a new Mn-containing enzyme.

    abstract::A manganese-containing superoxide dismutase (EC 1.15.1.1) was purified to homogeneity from a higher plant for the first time. The enzyme was isolated fromPisum sativum leaf extracts by thermal fractionation, ammonium sulfate salting out, ion-exchange and gel-filtration column chromatography, and preparative polyacryla...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00582359

    authors: Sevilla F,López-Gorgé J,Gómez M,Del Río LA

    更新日期:1980-01-01 00:00:00

  • Genome-wide identification, classification, and expression of phytocyanins in Populus trichocarpa.

    abstract:MAIN CONCLUSION:74 phytocyanin genes were identified in the Populus trichocarpa genome. Phylogenetic analysis grouped the PC proteins into four subfamilies (UCs, PLCs, SCs, and ENODLs). Closely related PC proteins share similar motifs, implying similar functions. Expression profiles of PtPC genes were analyzed in respo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2849-2

    authors: Luo S,Hu W,Wang Y,Liu B,Yan H,Xiang Y

    更新日期:2018-05-01 00:00:00

  • Accumulation of acidic SK₃ dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae.

    abstract::The role of acidic SK(n) dehydrins in stress tolerance of important crop and model species of the Solanaceae remains unknown. We have previously shown that the acidic SK₃ dehydrin DHN24 from Solanum sogarandinum is constitutively expressed and its expression is associated with cold acclimation. Here we found that DHN2...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-2018-6

    authors: Szabala BM,Fudali S,Rorat T

    更新日期:2014-04-01 00:00:00

  • Movement and metabolism of oligogalacturonide elicitors in tomato shoots.

    abstract::We have studied the movement and metabolism of oligogalacturonides through shoots of tomato (Lycopersicon esculentum L. cv Rutgers). Oligomers of polygalacturonic acid were prepared by enzyme digestion and gel filtration. These were end-reduced with [(3)H]NaBH4, using an improved reaction method, to yield oligoalditol...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197050

    authors: Macdougall AJ,Rigby NM,Needs PW,Selvendran RR

    更新日期:1992-11-01 00:00:00

  • Absence of major differences between soluble proteins from haploid gametophytes and diploid sporophytes in the green alga Ulva mutabilis Føyn.

    abstract::Soluble proteins from haploid gametophytes and diploid sporophytes of the marine green alga Ulva mutabilis Føyn have been reexamined, using polyacrylamide gel electrophoresis and isoelectric focusing. A two-dimensional system resolved about 150 protein spots. In contrast to an earlier report (Hoxmark (1976) Planta 130...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00393447

    authors: Hushovd OT,Gulliksen OM,Nordby O

    更新日期:1982-11-01 00:00:00

  • Kinetic properties of a micronutrient transporter from Pisum sativum indicate a primary function in Fe uptake from the soil.

    abstract::Fe uptake in dicotyledonous plants is mediated by a root plasma membrane-bound ferric reductase that reduces extracellular Fe(III)-chelates, releasing Fe(2+) ions, which are then absorbed via a metal ion transporter. We previously showed that Fe deficiency induces an increased capacity to absorb Fe and other micronutr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1156-7

    authors: Cohen CK,Garvin DF,Kochian LV

    更新日期:2004-03-01 00:00:00

  • Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis.

    abstract::MicroRNAs (miRNAs) are emerging as essential regulators of biological processes. Somatic embryogenesis is one of the most important techniques for gymnosperm-breeding programs, but there is little understanding of its underlying mechanism. To investigate the roles of miRNAs during somatic embryogenesis in larch, we co...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1643-9

    authors: Zhang J,Zhang S,Han S,Wu T,Li X,Li W,Qi L

    更新日期:2012-08-01 00:00:00

  • The plant LIM proteins: unlocking the hidden attractions.

    abstract:MAIN CONCLUSION:The plant LIMs comprise two sub-families with one (DA1/DAR) and two (2LIM) LIM domains. This review comprehensively discussed the structure and potential role of this protein family in diverse area of plant biology. The description of first eukaryote lineage-specific plant LIM domain (LIN11, ISL1, and M...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-017-2715-7

    authors: Srivastava V,Verma PK

    更新日期:2017-09-01 00:00:00

  • Towards understanding peroxisomal phosphoregulation in Arabidopsis thaliana.

    abstract:MAIN CONCLUSION:This work identifies new protein phosphatases and phosphatase-related proteins targeting peroxisomes, and raises the question of a novel protein import pathway from ER to peroxisomes involving peroxisomal targeting signal type 1 (PTS1) Plant peroxisomes are essential for several processes, for example l...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-015-2439-5

    authors: Kataya AR,Schei E,Lillo C

    更新日期:2016-03-01 00:00:00

  • Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.).

    abstract::Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00454448

    authors: Khavkin EE,Misharin SI,Polikarpochkina RT

    更新日期:1979-01-01 00:00:00

  • The role of bacterial attachment in the transformation of cell-wall-regenerating tobacco protoplasts by Agrobacterium tumefaciens.

    abstract::The presence of a newly formed primary cell wall was shown to be required for attachment and subsequent transformation of tobacco leaf protoplasts by Agrobacterium tumefaciens in cocultivation experiments. In these experiments both protoplasts at different stages after their isolation and cell-wall inhibitors were use...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00401165

    authors: Krens FA,Molendijk L,Wullems GJ,Schilperoort RA

    更新日期:1985-11-01 00:00:00

  • Gravistimulated asymmetries in the outer epidermal cell walls of graviresponding coleoptiles.

    abstract::Gravitropic plant growth is due to gravistimulated asymmetric extension rates of the affected flanks of the graviresponding organ. Differential growth of the upper and lower flanks (UFs, LFs) of graviresponding plant organs may in principle be achieved by various biochemical and/or biophysical asymmetries. The gravist...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/pl00008100

    authors: Edelmann HG

    更新日期:1997-09-01 00:00:00

  • Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings.

    abstract:MAIN CONCLUSION:This study highlights dehydration-mediated temporal changes in physicochemical, transcriptome and metabolome profiles indicating altered gene expression and metabolic shifts, underlying endurance and adaptation to stress tolerance in the marginalized crop, grasspea. Grasspea, often regarded as an orphan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-03082-2

    authors: Rathi D,Gayali S,Pareek A,Chakraborty S,Chakraborty N

    更新日期:2019-09-01 00:00:00

  • Metabolic energy for stomatal opening. Roles of photophosphorylation and oxidative phosphorylation.

    abstract::The supply of energy for stomatal opening was investigated with epidermal peels of Commelina communis L. and Vicia faba L., under white, blue and red irradiation or in darkness. Fluencerate response curves of stomatal opening under blue and red light were consistent with the operation of two photosystems, one dependen...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00395472

    authors: Schwartz A,Zeiger E

    更新日期:1984-05-01 00:00:00

  • Changes to the galactose/mannose ratio in galactomannans during coffee bean ( Coffea arabica L.) development: implications for in vivo modification of galactomannan synthesis.

    abstract::Endosperm was isolated from Arabica Caturra coffee beans 11, 15, 21, 26, 31 and 37 weeks after flowering, and the chemical composition and relative solubility of its component polysaccharides determined at each growth stage. Chemical analysis of the total mannan content of the cell wall material was done after solubil...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1003-x

    authors: Redgwell RJ,Curti D,Rogers J,Nicolas P,Fischer M

    更新日期:2003-06-01 00:00:00