Nitrate inhibits the remobilization of cell wall phosphorus under phosphorus-starvation conditions in rice (Oryza sativa).

Abstract:

MAIN CONCLUSION:NO3- not only inhibited the reutilization of cell wall P via decreasing root cell wall pectin content and PME activity, but also hampered the P translocation from root to shoot. The rice cultivars 'Kasalath' (Kas) and 'Nipponbare' (Nip) were used to demonstrate that the nitrogen source NO3- inhibits internal phosphorus (P) reutilization in rice under P-absence conditions. Analysis using Kas showed that the expression of - P-induced marker genes OsIPS1/2 and OsSPX1/2/3/5 are significantly higher under 1 mM NO 3-  - P (1N - P) treatment than 0 mM NO 3-  - P (0N - P) treatment. The absence of NO3- from the nutrient solution significantly increased cell wall P release by increasing pectin synthesis and increasing the activity of pectin methylesterase (PME), and also significantly improved the translocation of soluble P from the root to the shoot by increasing xylem sap P content under P-absence conditions. The rice seedlings grown in 0 mM NO3- accumulated significantly higher nitric oxide (NO) in the roots than those grown in 1 mM NO3-. Exogenously applying the NO donor sodium nitroprusside (SNP) revealed that NO is a major contributor to differential cell wall P remobilization in rice by mediating pectin synthesis and demethylation under different NO3- concentrations (0 and 1 mM) under P-deprived conditions.

journal_name

Planta

journal_title

Planta

authors

Zhu CQ,Zhu XF,Wang C,Dong XY,Shen RF

doi

10.1007/s00425-018-2892-z

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

185-196

issue

1

eissn

0032-0935

issn

1432-2048

pii

10.1007/s00425-018-2892-z

journal_volume

248

pub_type

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