Abstract:
MAIN CONCLUSION:The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-cysticercosis vaccine candidate. Cysticercosis is a zoonosis caused by Taenia solium that can be prevented by interrupting the parasite life cycle through pig vaccination. Several injectable vaccine candidates have been reported, but the logistic difficulties and costs for its application limited its use in nationwide control programs. Oral plant-based vaccines can deal with this limitation, because of their easy administration and low cost. A stable expression of the HP6/TSOL18 anti-T. solium cysticercosis protective antigen in carrot calli transformed with an optimized transgene is herein reported. An antigen accumulation up to 14 µg g(-1) of dry-weight biomass was achieved in the generated carrot lines. Mouse immunization with one of the transformed calli induced both specific IgG and IgA anti-HP6/TSOL18 antibodies. A statistically significant reduction in the expected number of T. crassiceps cysticerci was observed in mice orally immunized with carrot-made HP6/TSOL18, in a similar extent to that obtained by subcutaneous immunization with recombinant HP6/TSOL18 protein. In this study, a new oral plant-made version of the HP6/TSOL18 anti-cysticercosis vaccine is reported. The vaccine candidate should be further tested against porcine cysticercosis.
journal_name
Plantajournal_title
Plantaauthors
Monreal-Escalante E,Govea-Alonso DO,Hernández M,Cervantes J,Salazar-González JA,Romero-Maldonado A,Rosas G,Garate T,Fragoso G,Sciutto E,Rosales-Mendoza Sdoi
10.1007/s00425-015-2431-0subject
Has Abstractpub_date
2016-03-01 00:00:00pages
675-85issue
3eissn
0032-0935issn
1432-2048pii
10.1007/s00425-015-2431-0journal_volume
243pub_type
杂志文章相关文献
PLANTA文献大全abstract::Ethylene responsive factors (ERFs) are important plant-specific transcription factors, some of which have been demonstrated to interact with the ethylene-responsive GCC box and the dehydration-responsive element (DRE); however, data on the roles of ERF proteins in connection with various signaling pathways are limited...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1347-x
更新日期:2004-12-01 00:00:00
abstract::Two genomic clones ( OsMET1-1, AF 462029 and OsMET1-2, TPA BK001405), each encoding a cytosine-5 DNA methyltransferase (MTase), were isolated from rice ( Oryza sativa L.) BAC libraries. OsMET1-1 has an open reading frame of 4,566 nucleotides with 12 exons and 11 introns while OsMET1-2 has an open reading frame of 4,49...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-1112-6
更新日期:2004-01-01 00:00:00
abstract:MAIN CONCLUSION:Expression of bHLH104 - GFP driven by the MYB72 promoter improves plants' tolerance to Fe deficiency and increases seed Fe concentrations. Iron (Fe) deficiency causes reduced crop yield and quality. In humans, Fe deficiency is directly associated with Fe-deficiency anemia. Therefore, breeding Fe-deficie...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2703-y
更新日期:2017-09-01 00:00:00
abstract::The effects of O2, growth-regulators and desiccation on callus growth and somatic embryo (embryoid) development were investigated in cultures of immature embryos of two lines of Triticum aestivum L. Callus and embryoid formation were induced on media that contained N(6)-furfurylamin-opurine (kinetin) and either 2,4-di...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00396349
更新日期:1988-09-01 00:00:00
abstract::In Arabidopsis the in vitro culture of immature zygotic embryos (IZEs) at a late stage of development, on the solid medium containing synthetic auxin, leads to formation of somatic embryos via direct somatic embryogenesis (DSE). The presented results provide evidence that in IZE cells competent for DSE are located in ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0510-6
更新日期:2007-08-01 00:00:00
abstract::In roots of tomato ( Lycopersicon esculentum Mill.), extranumerary root hairs and transfer cell-like wall ingrowth depositions in the rhizodermis were developed in response to P and Fe deficiency. Immunocytolocalization of the plasma membrane H(+)-ATPase in roots of P-deficient plants revealed no appreciable increase ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0738-0
更新日期:2002-06-01 00:00:00
abstract::The roots of most extant plants are able to become engaged in an interaction with a small group of fungi of the fungal order Glomales (Glomeromycota). This interaction-arbuscular mycorrhizal (AM) symbiosis-is the evolutionary precursor of most other mutualistic root-microbe associations. The molecular analysis of this...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-004-1436-x
更新日期:2005-05-01 00:00:00
abstract:MAIN CONCLUSION:The plant LIMs comprise two sub-families with one (DA1/DAR) and two (2LIM) LIM domains. This review comprehensively discussed the structure and potential role of this protein family in diverse area of plant biology. The description of first eukaryote lineage-specific plant LIM domain (LIN11, ISL1, and M...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-017-2715-7
更新日期:2017-09-01 00:00:00
abstract::Spores of the ferns, Dryopteris filix-mas, D. paleacea and Polystichum minutum, sown on plain agar in quartz-distilled water, required several hours of red light in order to germinate. When, however, water agar was replaced by agar made up with a mineral nutrition medium, a single pulse of red light (about 1 min) was ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391026
更新日期:1985-05-01 00:00:00
abstract::Non-lignified fibre cells (named gelatinous fibres) are present in tension wood and the stems of fibre crops (such as flax and hemp). These cells develop a very thick S2 layer within the secondary cell wall, which is characterised by (1) cellulose microfibrils largely parallel to the longitudinal axis of the cell, and...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-005-0118-7
更新日期:2006-01-01 00:00:00
abstract::Plant root development is highly responsive both to changes in nitrate availability and beneficial microorganisms in the rhizosphere. We previously showed that Phyllobacterium brassicacearum STM196, a plant growth-promoting rhizobacteria strain isolated from rapeseed roots, alleviates the inhibition exerted by high ni...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-010-1264-0
更新日期:2010-11-01 00:00:00
abstract::Leaf tissue of the panicoid grass Pennisetum purpureum (Schum) and of the chloridoid grass Muhlenbergia montana (Hitchcock) were fractionated to produce preparations enriched in the contents of mesophyll and bundle sheath cells. Sucrose phosphate synthetase and sucrose synthetase were found predominantly in the mesoph...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385403
更新日期:1975-01-01 00:00:00
abstract::The moss Fontinalis antipyretica, an aquatic bryophyte previously described as desiccation-intolerant, is known to survive intermittent desiccation events in Mediterranean rivers. To better understand the mechanisms of desiccation tolerance in this species and to reconcile the apparently conflicting evidence between d...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1388-x
更新日期:2011-07-01 00:00:00
abstract::Two lectins were purified by affinity chromatography from mature peanut (Arachis hypogaea L.) nodules, and compared with the previously characterised seed lectin of this plant. One of the nodule lectins was similar to the seed lectin in its molecular weight and amino-acid composition and ability to bind derivatives of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392475
更新日期:1988-11-01 00:00:00
abstract::Uptake of silicic acid in the diatoms Navicula pelliculosa and Nitzschia alba was studied, using the natural isotope, (28)Si, or a radioisotope, (31)Si. The rate of uptake of silicic acid was also determined by using [(68)Ge]germanic acid as a tracer of silicic acid. At a given silicicacid concentration in the growth ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00389321
更新日期:1974-01-01 00:00:00
abstract::The pathway of sucrose metabolism in cyanobacteria is just starting to be elucidated. The present study describes the first isolation and biochemical characterization of a prokaryotic sucrose synthase (SS, EC 2.4.1.13). Two SS forms (SS-I and SS-II) were detected in Anabaena sp. strain PCC 7119. The isoform SS-II was ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050651
更新日期:1999-11-01 00:00:00
abstract::The crystal structure of NP24-I, an isoform of the thaumatin-like protein (TLP) NP24 from tomato, has been reported. A prominent acidic cleft is observed between domains I and II of the three-domain structure of this antifungal protein, a feature common to other antifungal TLPs. The defensive role of the TLPs has also...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0790-5
更新日期:2008-10-01 00:00:00
abstract::Water deficits consistently promote higher concentrations of anthocyanins in red winegrapes and their wines. However, controversy remains as to whether there is any direct effect on berry metabolism other than inhibition of growth. Early (ED) and late (LD) season water deficits, applied before or after the onset of ri...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0598-8
更新日期:2007-12-01 00:00:00
abstract::The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized (14)C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00402999
更新日期:1982-06-01 00:00:00
abstract::Fungal mycelium of the fly agaric (Amanita muscaria [L. ex Fr.] Hooker), and inoculated or noninoculated seedlings of Norway spruce (Picea abies [L.] Karst.) were grown aseptically under controlled conditions. In order to detect symbiosis-specific polypeptides ('ectomycorrhizins', see Hubert and Martin, 1988, New Phyt...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF01160722
更新日期:1992-03-01 00:00:00
abstract::The role of oxygen in the photoinactivation of the photosynthetic apparatus of Spinacia oleracea L. was investigated. Moderate irradiation (1200 μmol photons m(-2)s(-1)) of spinach leaves in an atmosphere of pure nitrogen caused strong inhibition of subsequently measured net CO2 assimilation, whereas considerably less...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392242
更新日期:1985-08-01 00:00:00
abstract::Seedlings of Amaranthus caudatus L. var. Pendula were used to study the influence of several treatment: white light, 3,4-dihydroxyphenylalanine (DOPA), kinetin, gibberellic acid (GA3) on betalains biosynthesis. The pigments, betacyanins and betaxanthins, were separated using a Sephadex G-15 column chromatography. Qual...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00380880
更新日期:1980-07-01 00:00:00
abstract::Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) rep...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1679-x
更新日期:2012-11-01 00:00:00
abstract::Short-term applications of very high concentrations of 1-naphthaleneacetic acid (NAA) to expiants from flower stalks of tobacco (Nicotiana tabacum L. cv. Samsun) induced flower-bud regeneration to the same extent as longer or continuous incubation on lower concentrations. The maximum number of flower buds per explant ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00198793
更新日期:1990-02-01 00:00:00
abstract::Conditions of soil drying and plant growth that lead to non-hydraulic inhibition of leaf elongation and stomatal conductance in maize (Zea mays L.) were investigated using plants grown with their root systems divided between two containers. The soil in one container was allowed to dry while the other container was kep...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397586
更新日期:1989-11-01 00:00:00
abstract:MAIN CONCLUSION:Expression of PAP genes is strongly coordinated and represents a highly selective cell-specific marker associated with the development of chloroplasts in photosynthetically active organs of Arabidopsis seedlings and adult plants. Transcription in plastids of plants depends on the activity of phage-type ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-2924-8
更新日期:2018-09-01 00:00:00
abstract::Phytochrome per fresh weight in the apical 0.5 cm of the 1.5-cm coleoptile was twice that in the basal 0.5 cm; phytochrome per dry weight and phytochrome per DNA did not vary significantly from the base to the apex. Ratios of phytochrome to fresh or dry weight in the 6-cm coleoptile were nearly constant in the basal 5...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386911
更新日期:1968-12-01 00:00:00
abstract::Developing wheat (Triticum aestivum L.) endosperm was examined using ultrathin sections prepared from tissues harvested at 5, 9, 16 and 25 d after flowering. Protein bodies were evident by 9 d and displayed a variety of membranous structures and inclusions. The Golgi apparatus was a prominent organelle at all stages, ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392442
更新日期:1988-11-01 00:00:00
abstract::The carpel- and stamen-specific AtAGIP promoter derived from the Arabidopsis AGAMOUS (AG) second intron/enhancer is ideal for engineering complete sterility but it is highly host-specific. To ascertain whether a chimeric promoter with similar tissue specificity can be created for species other than Arabidopsis, we iso...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-010-1120-2
更新日期:2010-04-01 00:00:00
abstract:MAIN CONCLUSION:The HRA2pl peptide expressed by transient transformation in N. tabacum plants is capable of inhibiting the binding of the human metapneumovirus to HEp-2 cells at the fusion stage. Human metapneumovirus (hMPV) is an agent responsible for acute respiratory infections that mainly affects children under 3 y...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2277-5
更新日期:2015-07-01 00:00:00