Crystal structure analysis of NP24-I: a thaumatin-like protein.

Abstract:

:The crystal structure of NP24-I, an isoform of the thaumatin-like protein (TLP) NP24 from tomato, has been reported. A prominent acidic cleft is observed between domains I and II of the three-domain structure of this antifungal protein, a feature common to other antifungal TLPs. The defensive role of the TLPs has also been attributed to their beta-1,3-glucanase activity and here too the acidic cleft is reported to play a vital role. NP24 is known to bind beta-glucans and so a linear beta-1,3-glucan molecule has been docked in the interdomain cleft of NP24-I. From the docked complex it is observed that the beta-glucan chain is so positioned in the cleft that a Glu and Asp residue on either side of it may form a catalytic pair to cause the cleavage of a glycosidic bond. NP24 has been reported to be an allergenic protein and an allergenic motif could be identified on the surface of the helical domain II of NP24-I. In addition, some allergenic motifs bearing high similarity/identity with some predicted Ig-E binding motifs of closely related allergenic TLPs like Jun a 3 (Juniperus ashei, from mountain cedar pollen) and banana-TLP have been identified on the molecular surface of NP24-I.

journal_name

Planta

journal_title

Planta

authors

Ghosh R,Chakrabarti C

doi

10.1007/s00425-008-0790-5

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

883-90

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

228

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings.

    abstract:MAIN CONCLUSION:This study highlights dehydration-mediated temporal changes in physicochemical, transcriptome and metabolome profiles indicating altered gene expression and metabolic shifts, underlying endurance and adaptation to stress tolerance in the marginalized crop, grasspea. Grasspea, often regarded as an orphan...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-03082-2

    authors: Rathi D,Gayali S,Pareek A,Chakraborty S,Chakraborty N

    更新日期:2019-09-01 00:00:00

  • Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation.

    abstract::The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to alpha-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. P...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1129-x

    authors: Ebmeier A,Allison L,Cerutti H,Clemente T

    更新日期:2004-03-01 00:00:00

  • Nucleic acid and protein synthesis and loss of vigour in germinating wheat embryos.

    abstract::A study has been made of the RNA and protein synthesising systems of wheat embryos isolated from seed lots having high viability but differing in vigour. The rate of RNA and protein synthesis in wheat embryos during the early hours of germination is related to the vigour of the seed lot. The imposition of a stress fac...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385609

    authors: Blowers LE,Stormonth DA,Bray CM

    更新日期:1980-11-01 00:00:00

  • The plasma membrane of the Funaria caulonema tip cell: morphology and distribution of particle rosettes, and the kinetics of cellulose synthesis.

    abstract::Freeze-fracturing of Funaria hygrometrica caulonema cells leads to a cleavage within the plasma membrane. The extraplasmatic and the plasmatic fracture faces differ in their particle density. The plasmatic fracture face in caulonema tip cells or in tip cells of side branches, but never in other caulonema cells, is fur...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00429759

    authors: Reiss HD,Schnepf E,Herth W

    更新日期:1984-04-01 00:00:00

  • Phytochrome quantitation in crude extracts of Avena by enzyme-linked immunosorbent assay with monoclonal antibodies.

    abstract::An enzyme-linked immunosorbent assay (ELISA), which uses both rabbit polyclonal and mouse monoclonal antibodies to phytochrome, has been adapted for quantitation of phytochrome in crude plant extracts. The assay has a detection limit of about 100 pg phytochrome (<1 fmol of monomer) and can be completed within 10 h. No...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00409143

    authors: Shimazaki Y,Cordonnier MM,Pratt LH

    更新日期:1983-12-01 00:00:00

  • Respiration induced by blue light.

    abstract::The high rate of respiration in Chlorella which is found after feeding with glucose or after a longer period of photosynthesis, declines in the dark in the course of hours to about a quarter of its original value. Blue-green light (λ<550 mμ) specifically counteracts this decline and maintains the high rate. This blue ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00380214

    authors: Kowallik W,Gaffron H

    更新日期:1966-03-01 00:00:00

  • Characterization of a plant (rice) translin and its comparative analysis with human translin.

    abstract:MAIN CONCLUSION:For the first time, a plant (rice) translin was characterized. The rice translin protein, which was octameric in native state, bound efficiently to single-stranded DNA and RNA. Translin, a DNA-/RNA-binding protein, is expressed in brain, testis and in certain malignancies. It is involved in chromosomal ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2092-4

    authors: Chittela RK,Gupta GD,Ballal A

    更新日期:2014-08-01 00:00:00

  • Photosynthetic electron transport adjustments in overwintering Scots pine (Pinus sylvestris L.).

    abstract::As shown before [C. Ottander et al. (1995) Planta 197:176-183], there is a severe inhibition of the photosystem (PS) II photochemical efficiency of Scots pine (Pinus sylvestris L.) during the winter. In contrast, the in vivo PSI photochemistry is less inhibited during winter as shown by in vivo measurements of deltaA8...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100522

    authors: Ivanov AG,Sane PV,Zeinalov Y,Malmberg G,Gardeström P,Huner NP,Oquist G

    更新日期:2001-08-01 00:00:00

  • ABA signaling rather than ABA metabolism is involved in trehalose-induced drought tolerance in tomato plants.

    abstract:MAIN CONCLUSION:Trehalose increased drought tolerance of tomato plants, accompanied by reduced water loss and closed stomata, which was associated with the upregulated ABA signaling-related genes expression, but not in ABA accumulation. Drought is one of the principal abiotic stresses that negatively influence the grow...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-019-03195-2

    authors: Yu W,Zhao R,Wang L,Zhang S,Li R,Sheng J,Shen L

    更新日期:2019-08-01 00:00:00

  • 30-year progress of membrane transport in plants.

    abstract::In the past 30 years enormous progress was made in plant membrane biology and transport physiology, a fact reflected in the appearance of textbooks. The first book dedicated to 'Membrane Transport in Plants' was published on the occasion of the 'International Workshop on Membrane Transport in Plants' held at the Nucle...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-006-0341-x

    authors: Hedrich R,Marten I

    更新日期:2006-09-01 00:00:00

  • Sugar concentrations along and across the Ricinus communis L. hypocotyl measured by single cell sampling analysis.

    abstract::Single cell sap sampling and analysis were used to measure the longitudinal and radial distribution of sucrose, glucose and fructose in the apical cell division zone and in the basal, elongated zone of the Ricinus hypocotyl. Sucrose and hexose increased in concentration from the apex to the base of the seedling axis. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0309-x

    authors: Verscht J,Tomos D,Komor E

    更新日期:2006-11-01 00:00:00

  • The effect of Cl(-) upon the sensitivity of starch-containing and starch-deficient stomata and guard cell protoplasts towards potassium ions, fusicoccin and abscisic acid.

    abstract::Chloride ions are necessary to compensate for the positively charged potassium ions imported into guard cells of Allium cepa L. during stomatal opening. Therefore an external Cl(-) supply of intact Allium plants is important. But high levels of chloride have been found to reduce the sensitivity of the starch-lacking s...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385013

    authors: Schnabl H

    更新日期:1978-01-01 00:00:00

  • Dipeptide transport in barley mesophyll vacuoles.

    abstract::The transport of [14C]glycyl-glycine (Gly-Gly) across the tonoplast of isolated barley vacuoles has been characterized. Uptake of the dipeptide Gly-Gly into barley mesophyll vacuoles was strongly increased by the addition of ATP, while Mg2+ inhibited the ATP-dependent fluxes. Inhibition of the vacuolar proton pump by ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00203640

    authors: Jamaï A,Gaillard C,Delrot S,Martinoia E

    更新日期:1995-01-01 00:00:00

  • Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion.

    abstract:MAIN CONCLUSION:A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion. Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-019-03106-5

    authors: Pierce ET,Graham BP,Stiff MR,Osborne JA,Haigler CH

    更新日期:2019-05-01 00:00:00

  • Properties of α-galactosidase II(2) from Vicia faba seeds.

    abstract::α-Galactosidase II(2) (MW ∼ 43 390) from resting Vicia faba L. seeds had been shown to possess D-glucose/D-mannose-specific lectin activity. Inhibition studies with monosaccharides and an examination of the effects of heat and pH on the catalytic and lectin activities of the enzyme indicate that the enzyme substrate a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00446377

    authors: Dey PM,Naik S,Pridham JB

    更新日期:1986-01-01 00:00:00

  • Correct glycosylation, Golgi-processing, and targeting to protein bodies of the vacuolar protein phytohemagglutinin in transgenic tobacco.

    abstract::We used a heterologous system (transgenic Nicotiana tabacum L.) to investigate the processing, assembly and targeting of phytohemagglutinin (PHA), the lectin of the common bean, Phaseolus vulgaris L. In the bean, this glycoprotein accumulates in the protein bodies of the storage parenchyma cells in the cotyledons, and...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392425

    authors: Sturm A,Voelker TA,Herman EM,Chrispeels MJ

    更新日期:1988-08-01 00:00:00

  • Purification and characterization of a soluble β-1,4-glucan from bean (Phaseolus vulgaris L.)-cultured cells dehabituated to dichlobenil.

    abstract::Bean cells habituated to grow in the presence of dichlobenil exhibited reduced cellulose and hemicellulose content and an increase in pectic polysaccharides. Furthermore, following the extraction of pectins and hemicelluloses, a large amount of neutral sugars was released. These sugars were found to be part of a solub...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1861-9

    authors: Alonso-Simón A,Encina AE,Seyama T,Kondo T,García-Angulo P,Álvarez JM,Acebes JL,Hayashi T

    更新日期:2013-06-01 00:00:00

  • Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots.

    abstract::Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthet...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0461-3

    authors: Innocenti G,Pucciariello C,Le Gleuher M,Hopkins J,de Stefano M,Delledonne M,Puppo A,Baudouin E,Frendo P

    更新日期:2007-05-01 00:00:00

  • Identification of a transcription factor specifically expressed at the onset of leaf senescence.

    abstract::The differential expression of genes was analyzed during leaf senescence in Arabidopsis thaliana (L.) Heynh., using suppression subtractive hybridization (SSH). In order to characterize the differential expression of regulatory genes, the analysis was performed at a very early time point when leaves first differed in ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000512

    authors: Hinderhofer K,Zentgraf U

    更新日期:2001-07-01 00:00:00

  • Expression of the β-subunit of β-conglycinin in seeds of transgenic plants.

    abstract::Soybean (Glycine max (L.) Merr.) seeds contain the storage protein β-conglycinin, encoded by a multigene family. β-Conglycinin consists of three subunits; α', α, and β. A genomic clone for a β-subunit of β-conglycinin has been characterized by restriction-enzyme mapping and hybrid selected in-vitro translation followe...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00398665

    authors: Bray EA,Naito S,Pan NS,Anderson E,Dubé P,Beachy RN

    更新日期:1987-11-01 00:00:00

  • Inhibition of plant cell membrane transport phenomena induced by zearalenone (F-2).

    abstract::Zearalenone (F-2), an estrogenic factor produced by a number of Fusarium spp., stimulates a leakage of electrolytes, β-cyanin and aminoacids from three plant tissues. F-2 inhibits rubidium uptake in roots of Zea mays L. and Beta vulgaris L. var. rubra. However the effect in the latter tissue is evident after long-term...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00389051

    authors: Vianello A,Macri F

    更新日期:1978-01-01 00:00:00

  • Cytoplasmic fibres associated with streaming and saltatory-particle movement in Heracleum mantegazzianum.

    abstract::The giant cells of the petiolar hairs of Heracleum mantegazzianum contain cytoplasmic fibres that are readily visible with phase contrast or interference contrast optics. These fibres are associated with saltatory particle movement and protoplasmic streaming in this cell and are believed to be the living image of simi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386612

    authors: O'Brien TP,McCully ME

    更新日期:1970-03-01 00:00:00

  • Two isoforms of Rubisco activase in cotton, the products of separate genes not alternative splicing.

    abstract::In several plant species, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase consists of two isoforms that are produced by alternative splicing of a pre-mRNA. Two forms of activase corresponding to the longer, redox-regulated alpha and the shorter, beta forms were detected immunologically in cotton (Go...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-002-0923-1

    authors: Salvucci ME,van de Loo FJ,Stecher D

    更新日期:2003-03-01 00:00:00

  • Identification of genes differentially expressed in ectomycorrhizal roots during the Pinus pinaster-Laccaria bicolor interaction.

    abstract::Ectomycorrhizal associations are of major ecological importance in temperate and boreal forests. The development of a functional ectomycorrhiza requires many genetic and biochemical changes. In this study, suppressive subtraction hybridization was used to identify differentially expressed genes in the roots of maritim...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1874-4

    authors: Flores-Monterroso A,Canales J,de la Torre F,Ávila C,Cánovas FM

    更新日期:2013-06-01 00:00:00

  • Involvement of nitrite in the nitrate-mediated modulation of fermentative metabolism and nitric oxide production of soybean roots during hypoxia.

    abstract::It is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1773-0

    authors: Oliveira HC,Salgado I,Sodek L

    更新日期:2013-01-01 00:00:00

  • The amount and integrity of mtDNA in maize decline with development.

    abstract::In maize and other grasses there is a developmental gradient from the meristematic cells at the base of the stalk to the differentiated cells at the leaf tip. This gradient presents an opportunity to investigate changes in mitochondrial DNA (mtDNA) that accompany growth under light and dark conditions, as done previou...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1802-z

    authors: Oldenburg DJ,Kumar RA,Bendich AJ

    更新日期:2013-02-01 00:00:00

  • Spatial and temporal changes in endogenous cytokinins in developing pea roots.

    abstract::Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Ra...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-008-0699-z

    authors: Stirk WA,Novák O,Václavíková K,Tarkowski P,Strnad M,van Staden J

    更新日期:2008-05-01 00:00:00

  • A critique of widely used normalization software tools and an alternative method to identify reliable reference genes in red clover (Trifolium pratense L.).

    abstract::Determination of appropriate reference genes is crucial to normalization of gene expression data and prevention of biased results in qRT-PCR studies. This study is the first attempt to systematically compare potential reference genes to detect the most constitutively expressed reference genes for accurate normalizatio...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1682-2

    authors: Mehdi Khanlou K,Van Bockstaele E

    更新日期:2012-11-01 00:00:00

  • [Studies on the association of 5-dehydroquinate hydro-lyase and shikimate: NADP(+)-oxidoreductase in higher plants].

    abstract::The properties of two enzymes involved in the shikimic acid pathway, dehydroquinate hydro-lyase and shikimate: NADP(+) oxidoreductase were studied in different species of higher plants (pteridophytes, gymnosperms, angiosperms) using chromatography on Sephadex G 100, DEAE cellulose, hydroxylapatite and isoelectric focu...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390823

    authors: Boudet AM,Lécussan R

    更新日期:1974-03-01 00:00:00

  • [The process of regeneration in a moss caulonema, studied by means of autoradiographic determination of DNA-synthesis].

    abstract::Ten-cell-long filaments of the caulonema of Funaria hygrometrica were isolated and labeled with (3)H-thymidine. During the process of regeneration this precursor is incorporated into the nucleus and the chloroplasts. The nuclei of aged cells are preferentially labeled, even the nuclei of such cells which probably will...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385072

    authors: Knoop B,Hahn H,Bopp M

    更新日期:1969-09-01 00:00:00