Abstract:
:In several plant species, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase consists of two isoforms that are produced by alternative splicing of a pre-mRNA. Two forms of activase corresponding to the longer, redox-regulated alpha and the shorter, beta forms were detected immunologically in cotton (Gossypium hirsutum L.) leaves, but their N-termini differed in 4 of 14 residues. The cDNAs for the alpha and beta forms of cotton activase diverged throughout the translated and 3'-untranslated regions, including variations that accounted for the differences in N-terminal amino acid sequence. Analysis of genomic DNA confirmed that separate genes encoded the alpha and beta forms of cotton activase. Separate activase genes were also detected in diploid species of cotton containing the different progenitor genomes of the cultivated allotetraploid, indicating that the occurrence of separate alpha- and beta-form genes in cotton predates the merger of the diploid genomes. The deduced amino acid sequences of the two forms of cotton activase exhibited 84% identity and both forms were active after expression in Escherichia coli. The recombinant alpha and beta forms exhibited similar affinities for ATP and only minor differences in thermotolerance, but their ATPase specific activities differed. The results show for the first time a plant species with two forms of activase that are structurally and functionally equivalent to the alternatively spliced alpha and beta forms in other plants, but that are encoded by separate genes. That cotton still expresses both forms of activase, even without alternative splicing, suggests that each form has a required function in photosynthesis.
journal_name
Plantajournal_title
Plantaauthors
Salvucci ME,van de Loo FJ,Stecher Ddoi
10.1007/s00425-002-0923-1subject
Has Abstractpub_date
2003-03-01 00:00:00pages
736-44issue
5eissn
0032-0935issn
1432-2048journal_volume
216pub_type
杂志文章相关文献
PLANTA文献大全abstract::We studied changes induced by cold on electron transfer pathways (linked to NADH or NADPH oxidation) in endoplasmic reticulum of rapeseed hypocotyls (Brassica napus L.) from a freezing-sensitive variety (ISL) and freezing-tolerant variety (Tradition). Plantlets were grown at 22 degrees C then submitted to a cold shock...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0793-2
更新日期:2008-10-01 00:00:00
abstract::The apparent photosynthetic affinity of A. variabilis to CO2 is greatly affected by the CO2 concentration in the medium during growth. Halfmaximal rate of photosynthetic O2 evolution is achieved at 10 μM and 100 μM inorganic carbon (Cinorg) in cells grown at low-CO2 (air) and high CO2 (5% v/v CO2 in air), respectively...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384557
更新日期:1980-08-01 00:00:00
abstract::Light-grown dwarf peas were disbudded except for a single lateral bud, then transferred to darkness at 24° C. During the dark period the seedlings were irradiated daily for 5 or 7 min with R or FR. The buds exposed to R developed into shoots faster than those irradiated with FR. The R effect was FR reversible, and the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387631
更新日期:1971-06-01 00:00:00
abstract::Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) rep...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1679-x
更新日期:2012-11-01 00:00:00
abstract::Hydrogen sulphide is an essential electron donor in the photosynthesis of sulphur bacteria. In addition, it has been known to be an inhibitor of photosynthesis in green plants. More specifically, it is assumed that sulphide interacts with the oxygen-evolving system. If the view is accepted that there is a more or less...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00539911
更新日期:1966-03-01 00:00:00
abstract::The qualitative and quantitative composition of the carotenoids of young cells of Euglena gracilis strain 1224-5/9 corresponded to that of the "Z"-strain.The frequently observed yellow-reddish discolouration of ageing cells was found to be caused by a heavy breakdown of the chlorophylls and not by an increased synthes...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387621
更新日期:1971-06-01 00:00:00
abstract::Glycosyltransferase complexes are known to be involved in plant cell wall biosynthesis, as for example in cellulose. It is not known to what extent such complexes are involved in biosynthesis of pectin as well. To address this question, work was initiated on ARAD1 (ARABINAN DEFICIENT 1) and its close homolog ARAD2 of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1592-3
更新日期:2012-07-01 00:00:00
abstract::The microtubular cytoskeleton has been studied during development of the zygote, proembryo and free-nuclear endosperm inA. thaliana using immunofluorescence localization of tubulin in enzymatically isolated material. Abundant micro tubules (MTs) are found throughout proembryogenesis. Microtubules in the coenocytic end...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF01102418
更新日期:1991-06-01 00:00:00
abstract::Rapid mobilisation of storage products, including xyloglucan, in cotyledons of germinating nasturtium (Tropaeolum majus L.) normally starts about 7-8 d after imbibition and growth of the seedling at 20-25° C. Levels of activity of endo-1,4-β-glucanase (EC 3.2.1.4) in cotyledons, as assayed viscometrically with xyloglu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197728
更新日期:1991-02-01 00:00:00
abstract:MAIN CONCLUSION:ATR-FTIR spectroscopy in combination with uni- and multivariate analysis was used to quantify the spectral-chemical composition of the leaf cuticle of pea, investigating the effects of variety and heat stress. Field pea (Pisum sativum L.) is sensitive to heat stress and our goal was to improve canopy co...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-3025-4
更新日期:2019-02-01 00:00:00
abstract::Nod factors are lipo-chito-oligosaccharides secreted by rhizobia that initiate many responses in the root hairs of the legume hosts, culminating in deformed hairs. The heterotrimeric G-protein agonists mastoparan, Mas7, melittin, compound 48/80 and cholera toxin provoke root hair deformation, whereas the heterotrimeri...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0900-8
更新日期:2003-02-01 00:00:00
abstract::Shampoo ginger (Zingiber zerumbet Smith) has a high content and large variety of terpenoids in the essential oil of its rhizome. Here, we report on the isolation of a cDNA clone (ZSS1) encoding alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis. This clone contains an open reading frame of 1,644 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0700-x
更新日期:2008-05-01 00:00:00
abstract::Rapid changes in gene expression were studied during incubation of cucumber (Cucumis sativus L.) cotyledons with cytokinins in darkness. Complementary-DNA clones for mRNAs whose levels decreased within 4 h of treatment with N6-benzyladenine (BA) were isolated by differential hybridization. One of them (CR9) was sequen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF02411564
更新日期:1994-01-01 00:00:00
abstract::Centrifugal accelerations of 50-250 g were applied to rhizoids of Chara globularis Thuill. at stimulation angles (alpha) of 5-90 degrees between the acceleration vector and the rhizoid axis. After the start of centrifugation, the statoliths were pressed asymmetrically onto the centrifugal flank of the apical cell wall...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195738
更新日期:1996-07-01 00:00:00
abstract::Scenedesmus obliquus, strain 633, which synthesizes ketocarotenoids and sporopollenin, also forms pink-red-colored cell walls. Both the cell walls left over after autospore liberation and those from homogenates of disrupted green cells have similar carotenoid pigmentation. Canthaxanthin, astaxanthin, an unidentified k...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395176
更新日期:1981-03-01 00:00:00
abstract:MAIN CONCLUSION:The analysis of early generations of triticale showed numerous rearrangements of the genome. Complexed transformation included loss of chromosomes, t-heterochromatin content changes and the emergence of retrotransposons in new locations. This study investigated certain aspects of genomic transformations...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-017-2827-0
更新日期:2018-04-01 00:00:00
abstract::Differentiation of shoots and roots or unorganized proliferation could be induced in tissue cultures of Solanum xanthocarpum when callus tissues habituated to a medium containing 2.4-D and meso-inositol were transferred to an auxinfree medium or to a medium in which the ratio of auxin and meso-inositol had been altere...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398022
更新日期:1968-12-01 00:00:00
abstract::Sucrose uptake into tonoplast vesicles, which were prepared from red beet (Beta vulgaris L.) vacuoles isolated by two different methods, was stimulated by MgATP. Using the same medium as for osmotic disruption of vacuoles, membrane vesicles were prepared from tissue homogenates of dormant red beet roots and separated ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194069
更新日期:1991-09-01 00:00:00
abstract::Redlight is known to increase the growth potential in the embryo of photodormant lettuce seed, enabling it to overcome the resistance offered by the seed coats (particularly the endosperm) or by an osmotically active medium. Determinations of the water potential of lettuce embryos germinating in osmoticum. carried out...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387687
更新日期:1971-03-01 00:00:00
abstract::Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0779-0
更新日期:2008-10-01 00:00:00
abstract::Quinolizidine alkaloids formed in the leaves of Lupinus albus L. are translocated via the phloem to the other plant organs, especially the maturing fruits. Compared with amino-acid transport in the phloem, the alkaloids contribute about 8% to the overall nitrogen being exported from the leaf. Since it is likely that t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00407083
更新日期:1984-11-01 00:00:00
abstract::Ribulose diphosphate (RuDP) and (PEP) phosphoenolpyruvate carboxylase enzyme activities were studied in young, mature, and senescent Portulaca oleracea leaves. While the absolute amount of both the C3 (RuDP) and C4 (PEP) carboxylase is less in senescent leaves than in mature leaves, RuDP carboxylase activity is reduce...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390316
更新日期:1976-01-01 00:00:00
abstract::Free porphyrins and their magnesium complexes, including chlorophylls, are potent photo-sensitizers. Plants usually accumulate these compounds bound to proteins together with protective compounds like carotenoids. Besides their protective role, carotenoids can play a structural role in these complexes. To analyze the ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0409-7
更新日期:2007-03-01 00:00:00
abstract::It is shown that simple, unbranched, plasmodesmata between young xylem ray cells of willow have no direct intercellular continuity apart from the plasmalemma which limits the cytoplasm and lines the plasmodesmatal canal. Each plasmodesma is traversed by a 200 Å diameter tubule (the desmotubule) which has a wall with p...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398199
更新日期:1968-09-01 00:00:00
abstract::To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, ea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1281-y
更新日期:2004-09-01 00:00:00
abstract::It is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1773-0
更新日期:2013-01-01 00:00:00
abstract::The permeability of biological membranes to boric acid was investigated using the giant internodal cells of the charophyte alga Chara corallina (Klein ex Will. Esk. R.D. Wood). The advantage of this system is that it is possible to distinguish between membrane transport of boron (B) and complexing of B by plant cell w...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000484
更新日期:2001-05-01 00:00:00
abstract::The endosperm of Euphorbia lagascae Spreng. seeds contains high levels of the epoxidated fatty acid vernolic acid ( cis-12-epoxyoctadeca-cis-9-enoic acid). To obtain transgenic oilcrops producing high levels of vernolic acid, better knowledge of its endogenous metabolism is needed. In this paper we study the gene acti...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-001-0717-x
更新日期:2002-05-01 00:00:00
abstract::A 78-amino acid insertion (L78) is found in the low-affinity type (L-form) of starch phosphorylase (L-SP, EC 2.4.1.1). This insertion blocks the starch-binding site on the L-SP molecule, and it decreases the binding affinity of L-SP toward starch. The computational analysis of the amino acid sequence on L78 predicts s...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0103-1
更新日期:2006-02-01 00:00:00
abstract::Germinating barley grains contain at least eight different peptidases: three carboxypeptidase (pH optima 4.8, 5.2, and 5.7), three aminopeptidases which act on aminoacyl-β-naphthylamides (pH opitima in the hydrolysis of di- and tripeptides at pH 5.8-6.5), and two peptidases which hydrolyse Ala-Gly and Leu-Tyr optimall...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386993
更新日期:1972-06-01 00:00:00